• BMB Reports
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• v.34 no.3
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• pp.201-205
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• 2001

Thionins are a family of low molecular weight cysteine-rich antimicrobial peptides. We isolated a cDNA encoding thionin gene from a flower bud cDNA library of Chinese cabbage (CFT). The gene contains 611 by nucleotides with 60 bp, and 150 by untranslated regions at its N- and C-terminal, respectively. The deduced amino acid sequence encoded 133 amino acids containing precursor polypeptide. The protein reveals that the precursor has a tripartite structure: a putative signal sequence at the N-terminus, followed by a mature thionin peptide, and a C-terminal acidic domain, which facilitates transport of the mature thionin through membrane. Genomic Southern blot analysis suggests that the CFT gene may be present as a single or two copy gene in the Chinese cabbage genome. Northern blot analysis shows that the gene is specifically expressed in flowers, but not in leaves, stems, or roots. When we analyzed the antifungal activity of the recombinant CFT protein, which was expressed in E. coli using the truncated cDNA region corresponding to the mature protein part, it was not active on fungal growth inhibition.

• Proceedings of the KIEE Conference
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• 1997.07a
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• pp.86-88
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• 1997

The detection of the normal zones in the coil winding and the initiation of the proper dump sequence have been one of the most important areas in the superconducting magnet technology. In this paper, the process to derive optimal dump sequence has been investigated through quench simulation and analysis of magnetically coupled superconducting magnet system. The magnet terminal voltage and maximum temperature rise in the quench initiated point are calculated with respect to various input variables such as operation current, dump resistance, etc. The experimental system is comprised of sc solenoidal coil, data aquisition device, external circuit breakers and dump resistor. The quench behavior of the magnet(e.g., temperature profile and the voltage signal) was measured. From this results, theoretical predictions were found to coincide with the experimental observations.

• The Plant Pathology Journal
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• v.25 no.4
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• pp.400-407
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• 2009

A pathogenesis-related protein (PgPR5) gene that isolated from the leaf of Panax ginseng was characterized. The ORF is 756 bp with a deduced amino acid sequence of 251 residues. The calculated molecular mass of the matured protein is approximately 27.5 kDa with a predicated isoelectric point of 7.80. A GenBank BlastX search revealed that the deduced amino acid of PgPR5 shares highest sequence similarity to PR5 of Actinidia deliciosa (80% identity, 87% similarity). PgPR5 has a C-terminal and N-terminal signal peptide, suggesting that it is a vacuolar secreted protein. The expression of PgPR5 under various environmental stresses was analyzed at different time points using real-time PCR. Our results reveal that PgPR5 is induced by salt stress, chilling stress, heavy metal, UV, and pathogen infection. These results suggest that the PgPR5 could play a role in the molecular defence response of ginseng to abiotic and pathogen attack. This is the first report of the isolation of PR5 gene from the P. ginseng.

• Journal of Microbiology and Biotechnology
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• v.13 no.6
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• pp.859-865
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• 2003

Surfactin is a mixture of cyclic lipopeptides built from variants of a heptapeptide and a ${\beta}-hydroxy$ fatty acid produced by several strains of Bacillus sp. Surfactin isoforms produced by endophytic Bacillus sp. CY22 from a balloon flower were isolated and characterized. It was found that the purified surfactin had three isoforms with protonated masses of m/z 1,008, 1,022, and 1,036, and different structures in combination with Na, K, Ca ions using MALDI-TOF MS, ESI-MS/MS, and ICP MS, respectively. In the MS/MS analysis, the isolated surfactin had the identical amino acid sequence (LLVDLL) and hydroxy fatty acids (with 13 to 15 carbons in length), even though isolated from different Bacillus strains. The sfp22 gene, required for producing the surfactin, consisted of an open reading frame (ORF) of 675 bp encoding 224 amino acid residues with a signal peptide of 20 amino acids. The predicted amino acid sequence of sfp22 was very similar to that of Ipa-8.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.157-167
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• 2000

A thermostable chitosanase gene from the isolated strain, Bacillus sp. CK4, was cloned, and its complete DNA sequence was determined. The thermostable chitosanase gene was composed of an 822-bp open reading frame which encodes a protein of 242 amino acids and a signal peptide corresponding to a 30 kDa enzyme in size. The deduced amino acid sequence of the chitosanase from Bacillus sp. CK4 exhibits 76.6%, 15.3%, and 14.2% similarities to those from Bacillus subtilis, Bacillus ehemensis, and Bacillus circulans, respectively. C-terminal homology analysis shows that Bacillus sp. CK4 belongs to the Cluster III group with Bacillus subtilis. The size of the gene was similar to that of a mesophile, Bacillus subtilis showing a higher preference for codons ending in G or C. The functional importance of a conserved region in a novel chitosanase from Bacillus sp. CK4 was investigated. Each of the three carboxylic amino acid residues were changed to E50D/Q, E62D/Q, and D66N/E by site-directed mutagenesis. The D66N/E mutants enzymes had remarkably decreased kinetic parameters such as $V_{max}$ and k$\sub$cat/, indicating that the Asp-66 residue was essential for catalysis. The thermostable chitosanase contains three cysteine residues at position 49, 72, and 211. Titration of the Cys residues with DTNB showed that none of them were involved in disulfide bond. The C49S and C72S mutant enzymes were as stable to thermal inactivation and denaturating agents as the wild-type enzyme. However the half-life of the C211S mutant enzyme was less than 60 min at 80$^{\circ}C$, while that of the wild type enzyme was about 90 min. Moreover, the residual activity of C211S was substantially decreased by 8 M urea, and fully lost catalytic activity by 40% ethanol. These results show that the substitution of Cys with Ser at position 211 seems to affect the conformational stability of the chitosanase.

• Journal of Communications and Networks
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• v.11 no.1
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• pp.11-19
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• 2009

Multicarrier direct sequence code division multiple access (MC DS-CDMA) is an attractive technique for achieving high data rate transmission. This is valid regardless of whether or not the potentially large peak-to-average power ratio (PAPR) is an important factor for its application. On the other hand, code select CDMA (CS-CDMA) is an attractive technique with constant amplitude transmission of multicode signal regardless of subchannels. This is achieved by introducing a code select method. In this paper, we propose a new multiple access scheme based on the combination of MC DS-CDMA and CS-CDMA. The proposed scheme, which we call MC CS-CDMA, includes as special cases the subclasses of MC DS-CDMA and CS-CDMA. This paper investigates the performance of these systems over a multipath frequency selective fading channel using a RAKE receiver with maximal ratio combiner. In addition, the PAPR of the proposed system is compared with that of both MC DS-CDMA and CS-CDMA. Simulation results demonstrate that the proposed system provides better PAPR reduction than MC DS-CDMA, at the expense of the complexity of the receiver and the number of available users. The numerical result demonstrates that the proposed system has better performance than MC DS-CDMA due to the increased processing gain and time diversity gain.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.6C
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• pp.565-571
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• 2007

In order to improve PAPR (Peak-to-Average Power Ratio) reduction performance of the conventional SLM (Selective Mapping) for OFDM (Orthogonal Frequency Division Multiplexing) signals, we propose an effective SLM-PRSC (PAPR Reduction Sub-Carrier) hybrid scheme. In the proposed scheme, after performing the SLM for the frequency domain OFDM symbol excluding pre-determined PRSC positions, the SLM-PRSC hybrid sequence with the lowest PAPR generated by adding the time domain PRSC sequence to the results of the SLM, is selected as the transmitted OFDM signal. Since the identical PRSC sequences generated a priori are repeatedly used for every OFDM symbol, excessive IFFT (inverse Fast Fourier Transform) calculation is avoided. Simulation results show that the proposed scheme significantly improves the PAPR reduction performance of the conventional SLM, while avoiding excessive increase of IFFT calculation and the overhead for the SLM.

• Journal of Veterinary Clinics
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• v.21 no.3
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• pp.253-258
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• 2004

DNA double-strand break (DSB) is a serious treat for the cells including mutations, chromosome rearrangements, and even cell death if not repaired or misrepaired. Ku heterodimer regulatory DNA binding subunits (Ku70/Ku80) bound to double strand DNA breaks are able to interact with 470-kDa DNA-dependent protein kinase catalytic subunit (DNA-PKcs), and the interaction is essential for DNA-dependent protein kinase (DNA-PK) activity. The Ku80 mutants were designed to bind Ku70 but not DNA end binding activity and the peptides were treated in breast cancer cells for co-therapy strategy to see whether the targeted inhibition of DNA-dependent protein kinase (DNA-PK) activity sensitized breast cancer cells to ionizing irradiation or chemotherapy drug to develop a treatment of breast tumors by targeting proteins involved in damage-signaling pathway and/or DNA repair. We designed domains of Ku80 mutants, 26 residues of amino acids (HN-26) as a control peptide or 38 (HNI-38) residues of amino acids which contain domains of the membrane-translocation hydrophobic signal sequence and the nuclear localization sequence, but HNI-38 has additional twelve residues of peptide inhibitor region. We observed that the synthesized peptide (HNI-38) prevented DNA-PKcs from binding to Ku70/Ku80, resulting in inactivation of DNA-PK complex activity in breast cancer cells (MDA-465 and MDA-468). Consequently, the peptide treated cells exhibited poor to no DNA repair, and became highly sensitive to irradiation or chemotherapy drugs. The growth of breast cancer cells was also inhibited. These results demonstrate the possibility of synthetic peptide to apply breast cancer therapy to induce apoptosis of cancer cells.

• Journal of IKEEE
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• v.11 no.1 s.20
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• pp.30-37
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• 2007

This paper proposes a detection method of position of OFF-switch. Each switch has the parallel path with a serial combination of passive element, its parallel path has each different frequency characteristics. Frequency characteristic of ON-switch reveals a flat spectrum irrelevant to frequency characteristic of passive element connected in parallel to its each terminal and frequency characteristic of OFF-switch reveals the same characteristic as one of passive element connected in parallel. Detection of position of OFF-switch is done by measuring the similarity of each spectrum corresponding to frequency characteristic of passive element connected in parallel to OFF-switch. The measure of their similarity is to calculate Euclidean distance between their test spectrum and reference spectrum. The spectrum with the smallest distance among reference spectrum is recognized as the spectrum of OFF-switch. The real time digital signal processing system is implemented to detect the position of OFF-switch by using spectrum matching.

• 전자공학회논문지 IE
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• v.43 no.2
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• pp.70-74
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• 2006

The performance of conventional direct sequence code division multiple access (DS-CDMA) systems is decreased under environments such as additive white Gaussian noise (AWGN), channel distortion and interference noise due to multiple access user. By means of this parameter, auto correlation value of pseudo noise spreading sequence is decreased at receiver. This techniques which are based on correlation of between signature waveform signal. In this paper, to improve correlation property, we proposed the binary chirp DS-CDMA techniques which combine the DS-CDMA and chirp modulation. The proposed system which is based on binary chirp symbol has a good correlation value. Thus, we called BC DS-CDMA. To evaluate the system's performance, we compare the performance of the proposed systems with DS-CDMA systems under AWGN channel and halogen noise which exists on the powerline. The simulation results show that the proposed method has better performance than conventional technique.