• Journal of Microbiology and Biotechnology
• /
• 제5권3호
• /
• pp.117-124
• /
• 1995

A gene (xynA) encoding the endo-xylanase (E.C.3.2.1.8) from Bacillus stearothermophilus was cloned in E. coli, and its complete nucleotide sequence was determined. The xynA gene consists of a 636 base pairs open reading frame coding for a protein of 212 amino acids with a deduced molecular weight of 23, 283 Da. A putative signal sequence of 27 amino acid residues shows the features comparable with the Bacillus signal sequences; namely, the signal contains a positively charged region close to the N-terminus followed by a long hydrophobic string. The coding sequence is preceded by a possible ribosome binding site with a free energy value of -16.6 kcal/mol and the transcription initiation signals are located further upstream. The translation termination codon (TAA) at the 3 end of the coding sequence is followed by two palindrome sequences, one of which is thought to act as a terminator. The xynA gene has a high GC content, especially in the wobble position of codons (64%). Comparison of the primary protein sequence with those of other xylanases shows a high homology to the xylanases belonging to family G.

• Preventive Nutrition and Food Science
• /
• 제15권4호
• /
• pp.360-363
• /
• 2010

To enhance the expression and secretion of pediocin PA-1 from heterologous bacterial hosts, the promoter and deduced signal sequence (PS) of an $\alpha$-amylase gene from a Bifidobacterium adolescentis strain was fused with pediocin PA-1 structural and immunity genes (AB) and the resulting functions were evaluated in Escherichia coli. Two recombinant PCR products were created-one with just the deduced signal sequence and one with the sequence plus the Ser and Thr sequences that are the next two amino acids of the signal sequence. These two products, the PSAB (---AQA::KYY---) and PSABST (---AQA$\underline{ST}$::KYY---), respectively, were inserted into a TA cloning vector (yT&A) and named pPSAB, which was previously reported, and pPSABST. The two recombinant plasmid DNAs were transferred into E. coli JM109 and the transformants displayed antimicrobial activity, where the activity of E. coli JM109 (pPSAB) was stronger than that of E. coli JM109 (pPSABST), indicating that the ST amino acid residues were not necessary for secretion and might have even decreased the antimicrobial activity of recombinant pediocin PA-1.

• 전자공학회논문지SC
• /
• 제37권3호
• /
• pp.1-8
• /
• 2000

이 논문에서는 NN 필터를 이용한 표적추적을 위한 최적의 신호 강도 및 표적 검출 문턱값을 구하였다. 이를 위하여 먼저 HYCA 방식을 이용하여 NN 필터의 추적성능을 예측할 수 있도록 하고, 이것에 기초하여 예측된 추적성능과 신호 강도 및 표적 검출 문턱값 사이의 관계를 나타내었다. 그리고 이러한 관계를 이용하여 다음과 같은 다양한 비용에 대한 최적 파라미터를 얻었다： (1)위치 추정 오차 분산 합을 최소화하는 최적의 표적 검출 문턱값 순열(sequence)； (2)유효 게이트 면적 합을 최소화하는 최적의 표적 검출 문턱값 순열； (3)표적 신호 강도 합을 최소화하는 최적 표적 신호 강도 및 표적 검출 문턱값 순열.

• Journal of Microbiology and Biotechnology
• /
• 제24권3호
• /
• pp.337-345
• /
• 2014

Pichia pastoris is one of the most widely used expression systems for the secretory expression of recombinant proteins. The secretory expression in P. pastoris usually makes use of the prepro $MAT{\alpha}$ sequence from Saccharomyces cerevisiae, which has a dibasic amino acid cleavage site at the end of the signal sequence. This is efficiently processed by Kex2 protease, resulting in the secretion of high levels of proteins to the medium. However, the proteins that are having the internal accessible dibasic amino acids such as KR and RR in the coding region cannot be expressed using this signal sequence, as the protein will be fragmented. We have identified a new signal sequence of 18 amino acids from a P. pastoris protein that can secrete proteins to the medium efficiently. The PMT1-gene-inactivated P. pastoris strain secretes a ~30 kDa protein into the extracellular medium. We have identified this protein by determining its N-terminal amino acid sequence. The protein secreted has four DDDK concatameric internal repeats. This protein was not secreted in the wild-type P. pastoris under normal culture conditions. We show that the 18-amino-acid signal peptide at the N-terminal of this protein is useful for secretion of heterologous proteins in Pichia.

• 생명과학회지
• /
• 제20권11호
• /
• pp.1577-1581
• /
• 2010

조혈촉진 cytokine인 Granulocyte colony stimulating factor (G-CSF)는 골수세포를 자극하여 granulocyte로 증식, 분화시키는 기능을 가지며, 현재 아주 고가의 치료제로 사용되고 있다. 인간 G-CSF (hG-CSF)를 아직 시도되지 않은 누에 유래 세포주인 BM5 세포에서 발현시키고 생산 효율을 높이기 위해 hG-CSF cDNA를 변형하였다. hG-CSF의 cDNA의 endoplasmic reticulum (ER) signal sequence 부분을 누에의 소포체에서 분비되는 단백질인 prophenoloxidase (PPAE), protein disulfide isomerase (PDI)와 bombyxin (BX)에서 유래한 누에특이 ER signal sequence로 대체한 hG-CSF의 cDNA 함유 벡터를 구축하였다. 이들 벡터를 사용하여 형질전환한 BM5 세포의 배양액에 분비된 G-CSF 단백질을 western blot으로 분석하여 발현을 확인하였다. 누에특이 ER signal sequence들로 대체된 hG-CSF cDNA를 포함하는 벡터에 의한 hG-CSF 단백질 생산이 인간 G-CSF cDNA가 든 벡터에 의한 hG-CSF의 생산보다 월등히 효율적이었다. 또한, PPAE-signal sequence를 포함하는 hG-CSF 단백질은 배양배지에서 형질전환 4일 후에 최고에 달하였고, 7 일째까지 비슷한 양이 배지 내에서 검출되었다. 이상의 결과는 인간유래 유전자가 곤충세포 내에서 발현 될 때 인간유래 유전자 보다는 곤충 유전자발현 시스템에 맞게 변형했을 경우 더 효율적인 단백질 발현을 얻을 수 있음을 보여 준다.

• Journal of Communications and Networks
• /
• 제16권1호
• /
• pp.92-97
• /
• 2014

A novel method for extracting frequency slippage signal from radar full pulse sequence is presented. For the radar full pulse sequence received by radar interception receiver, radio frequency (RF) and time of arrival (TOA) of all pulses constitute a two-dimensional information sequence. In a complex and intensive electromagnetic environment, the TOA of pulses is distributed unevenly, randomly, and in a nonstationary manner, preventing existing methods from directly analyzing such time series and effectively extracting certain signal features. This work applies Gaussian noise insertion and structure function to the TOA-RF information sequence respectively such that the equalization of time intervals and correlation processing are accomplished. The components with different frequencies in structure function series are separated using empirical mode decomposition. Additionally, a chaos detection model based on the Duffing equation is introduced to determine the useful component and extract the changing features of RF. Experimental results indicate that the proposed methodology can successfully extract the slippage signal effectively in the case that multiple radar pulse sequences overlap.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 1998년도 하계종합학술대회논문집
• /
• pp.847-850
• /
• 1998

One of the most difficult problem of designing VLSI is a mixed-circuit design, that is to design circuit containing both analog parts and digital parts. Digital to analog converter and analog to digital converter is a typical case. Especially it can be a serious problem when mixed circuit are put into a large digital circuit like microcontroller. However nowadays this problem is settled by separating analog circuit parts outside the IC. This technique is based on converting a digital signal into a pulse sequence. Then an analog signal is obtained by averaging this pulse sequence at the external low-pass filter. An anlog to digital converter is designed using a stochastic logic instead of a traditional PWM (pulse-width modulation) signal and ins implemente dusing FPGa. Stochastic pulse sequence can be made as a simple circuits and moreover can be mathematically processed by simple circuits -AND gates. The spectral property of stochastic pulse sequence method is better than that of PWM method. So it make easy to design a external low-pass filter. This technique has important advantages, especially the reduction of the ADC cost.

• Journal of Microbiology and Biotechnology
• /
• 제9권5호
• /
• pp.576-581
• /
• 1999

Effects of signal sequences, protein sizes and dissolved oxygen on the secretion of human lysozyme from a recombinant yeast were experimentally characterized. The systems consisted of Saccharomyces cerevisiae host SEY2102 that was transformed with two different plasmids. These plasmids were identical with an exception to the plasmid pMC614, which contained the native yeast MFα1 sequence and the plasmid pMC632 with the non-native rat α-amylase signal sequence. The expression of human lysozyme was controlled by the ADHI promoter. The native yeast MFαl signal sequence was more efficient than the non-native rat α-amylase signal sequence in directing the secretion of human lysozyme. Lysozyme secreted with the α-amylase signal was retained inside the cells and released to the medium very slowly, thereby causing a lower cell growth rate and a decreased product secretion rate. Lysozyme was secreted more efficiently than invertase, which is an order of magnitude bigger in molecular size compared to lysozyme, which was under the direction of the MFαl signal sequence, suggesting that protein sizes may affect the secretion efficiency. When expressed in anaerobic conditions in the medium where the ADHI promoter was derepressed, the amount of lysozyme secreted was about twice higher than that of the aerobic culture. However, the secretion rates were identical. This result showed that the dissolved oxygen level may affect the efficiency of protein secretion only, and not the secretion rate of the product protein.

• 한국통신학회논문지
• /
• 제24권9A호
• /
• pp.1339-1345
• /
• 1999

본 논문에서는 랜덤시퀀스와 Hadamard 행렬을 이용하여 다중 정보를 은폐시킬 수 있는 새로운 디지털 제안하였다. 기존의 디지털 정보은폐에 사용된 기법은 하나의 랜덤시퀀스를 정보신호에 직접 곱하여 정보신호에 에너지 레벨을 매우 낮춤으로서 불법 이용자로 하여금 은폐된 정보의 검출이나 교란을 어렵게 하였다. 그러나 여러 개의 정보를 동일한 디지털 영상에 은폐시킬 경우에는 직교성이 보장되는 은폐코드를 사용해야 하기 때문에 어느 정도 상관성을 갖는 랜덤시퀀스만으로는 정보은폐가 어렵다. 따라서, 본 논문에서는 랜덤시퀀스의 장점과 더불어 직교성을 갖을 수 있도록 같은 행렬을 곱할 때만 정확한 신호로 추출되는 Hadamard 행렬을 랜덤시퀀스와 함께 사용하여 다수의 은폐정보에 대해서도 정확하게 은폐된 신호를 추출할 수 있는 새로운 디지털 정보은폐 방법을 제안하였다.

• 전자공학회논문지
• /
• 제53권7호
• /
• pp.85-91
• /
• 2016

레이더 시스템은 방사 신호의 탐지를 회피하기 위해 펄스 반복주기(PRI, Pulse Repetition Interval)와 PRI 패턴을 변조하고 있으며, 반대로 레이더 신호 탐지 시스템은 다양한 노력을 기울여 PRI와 PRI 패턴을 감지하려고 한다. 일반적으로 레이더 신호의 PRI 패턴을 검출하기 위해 펄스열의 도착시각에 대한 히스토그램 또는 자기 상관관계 기법으로 펄스 변조를 검출하고 있다. 본 논문에서는 유한 상태 머신 개념을 도입하여 펄스 반복주기를 검출하는 알고리즘을 제안한다. 이 알고리즘은 PRI 순서와 PRI 패턴을 찾을 수 있는 특징이 있다.