• Title/Summary/Keyword: sigma factor

Search Result 282, Processing Time 0.032 seconds

Sequence Analysis of $\beta$-Xylosidase Gene from Bacillus stearothermophilus (Bacillus stearothermophilus $\beta$-Xylosidase 유전자의 염기 서열 결정 및 분석)

  • 오현주;최용진
    • Microbiology and Biotechnology Letters
    • /
    • v.22 no.2
    • /
    • pp.134-142
    • /
    • 1994
  • The neucleotide sequences of the xylA gene encoding $\beta $-xylosidase of Bacillus stearothermophilus and is its flanking regions were datermined. Three open reading frame(ORFs) were found, one of which(ORF1) appeared to code for the $\beta $-xylosidase. The 1830 base pair ORF1 encoded 609 amino acids starting from a TTG initiation codon. The molecular weight deduced from the nucleotide sequence(68 KD) was in agreement with that estimated by SDS-polyacrylamide gel electrophoresis of the purified enzyme(66 KD). The Shine-Dalgarno sequence(5'-AGGAGG-3') was found 11 bp upstream of the initiation codon. Further 15 bp upstream, there observed a potential transcription initiation signals. The putative -10 sequence(CATAAT) and -35 sequence(TTGTTA) coresponded closely to the consensus sequences for Bacillus subtilis RNA polymerase with major sigma factor. The guanine-plus-cytosine content of the coding region of the xylA gene was 56mol% while that of the third position of the codons was 63 mol%. Based on the comparison with the amino acid sequences of several other carbohydrate degrading enzymes, two conserved regions, possibly participating in the catalytic mechamism of $\beta $-xylosidase xylA, were identified in 278-298 and 329-350 regions of the translated xylA gene. The nucleotide sequence of the xylA was found to exhibit no homology to any other genes so far reproted.

  • PDF

Surcharge Loading Effects on Slopes Using Finite Element Analysis (유한요소해석을 통한 사면 상재하중 영향 연구)

  • Jeon, Sang-Soo;Lee, Choong-Ho;Pham, Nguyen Quoc;Oh, Mi-Hee;Kim, Doo-Seop;Kang, Sang-Wook
    • Proceedings of the Korean Geotechical Society Conference
    • /
    • 2005.03a
    • /
    • pp.838-845
    • /
    • 2005
  • Slope stability analyses have been carried out to investigate surcharge loading effects. Finite Element Analysis (FEA) involves the stress-strain behaviour of soils achieving reasonably accurate and useful results of slope stability analysis. Therefore, in this study, one of well known FEA programs, SIGMA/W, has been used to do slope stability analyses with respect to various upper slope angles and surcharge loadings. Factor of Safety(FS) exponentially decreases and significantly good correlation with the increased slope angle for upper slopes. As the surcharge loading increases from 10 t/m to 90 t/m for nail-reinforced slopes, the FS in fully saturated condition decreases from 42% to 47% and from 17% to 25% for relatively low and high strength of soils, respectively, than in dry condition.

  • PDF

Initiation and Propagation Behaviors of Micro-Surface-Fatigue Cracks under In-Plane Tension Fatigue Tests (引張 軸荷重 疲勞 에 의한 微小表面 균열 의 發생 . 成長擧動)

  • 서창민
    • Transactions of the Korean Society of Mechanical Engineers
    • /
    • v.9 no.1
    • /
    • pp.1-9
    • /
    • 1985
  • In-plane tension fatigue tests (R = 0.05) were carried out to investigate the initiation and propagation behaviors of micro-surface-fatigue cracks on smooth surfaces of a mild steel. Also, the investigations of saturated cyclic strain which can be obtained by the fatigue tests have been made via the cyclic strain intensity factor, .DELTA. $K_{\epsilon}$/, for the purpose of unifying two approaches of the study of fatigue; the one approach is based on the fracture mechanics concept and the other on lowcycle fatigue concept. Some of the results are as follows; The growth rate, d(2a)/dN, of small cracks cannot be represented by one straight line as a function of .DELTA.K for various of the nominal stress range, .DELTA..sigma., and is higher than that of a larger through crack. The rearrangement of the d(2a)/dN by .DELTA..epsilon..root..pi.s( = .DELTA. $K_{\epsilon}$/) with the stress range .DELTA..epsilon. in .DELTA.K replaced by .DELTA..epsilon., strain range, gives one straight line of the .DELTA. $K_{\epsilon}$-d(2a)/dN relation for various values of stress range .DELTA.$_{\epsilon}$../.X>../.

On the mixed-mode crack propagation in FGMs plates: comparison of different criteria

  • Nabil, Benamara;Abdelkader, Boulenouar;Miloud, Aminallah;Noureddine, Benseddiq
    • Structural Engineering and Mechanics
    • /
    • v.61 no.3
    • /
    • pp.371-379
    • /
    • 2017
  • Modelling of a crack propagating through a finite element mesh under mixed mode conditions is of prime importance in fracture mechanics. In this paper, two crack growth criteria and the respective crack paths prediction in functionally graded materials (FGM) are compared. The maximum tangential stress criterion (${\sigma}_{\theta}-criterion$) and the minimum strain energy density criterion (S-criterion) are investigated using advanced finite element technique. Using Ansys Parametric Design Language (APDL), the variation continues in the material properties are incorporated into the model by specifying the material parameters at the centroid of each finite element. In this paper, the displacement extrapolation technique (DET) proposed for homogeneous materials is modified and investigated, to obtain the stress intensity factors (SIFs) at crack-tip in FGMs. Several examples are modeled to evaluate the accuracy and effectiveness of the combined procedure. The effect of the defects on the crack propagation in FGMs was highlighted.

A Study on Liquefaction Potential at Reclained Land by Hydraulic Hammer Compaction (유압햄머다짐 준설매립지반의 액상화평가에 관한 연구)

  • 김종국;김영웅;최인걸;최원호
    • Proceedings of the Korean Geotechical Society Conference
    • /
    • 2001.03a
    • /
    • pp.517-524
    • /
    • 2001
  • In this study, the effect of relative density and fine contents(Finer then # 0.08mm sieve) on liquefaction phenomenon in reclaimed land by hydraulic hammer compaction is analyzed. For more site-specific studies, reclaimed land in Inchon International Airport is selected and the cyclic triaxial tests are performed on disturbed samples. In cyclic triaxial tests, the characteristics of reclaimed land in Inchon International Airport are considered sufficiently. The liquefaction resistance stress ratio ($\tau$$\ell$/$\sigma$v') can be defined by relative density 40, 50, 60, and 70% and also by fine contents : 0, 10, 20, 30, and 40% under relative density (D$\_$r/) 50% used disturbed samples. From tile result of comparing tile cyclic triaxial tests, it is shown that the liquefaction strength of soil increases with increases of relative density and fine contents. Fspecially fine contents is the main factor affecting the liquefaction potential. In addition, the liquefaction resistance stress ratio is reduced by the increase of fine content and tile ratio of change is steep until fine contents 20% and that is flexible during the range of fine contents 20% to 40%. Through this study, it is proved that the soil characteristics (fine contents 5∼20%) of the reclaimed land in Inchon International Airport flays an important role in the reduction of liquefaction potential.

  • PDF

Fabrication and Characteristics of $n-CdS_{0.69}Se_{0.31}/p-Cu_{2-x}S_{0.69}Se_{0.31}$ Heterojunction Solar Cell ($n-CdS_{0.69}Se_{0.31}/p-Cu_{2-x}S_{0.69}Se_{0.31}$ Heterojunction 태양전지의 제작과 특성)

  • Baek, Seung-Nam;Hong, Kwang-Joon
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 2004.04b
    • /
    • pp.51-55
    • /
    • 2004
  • $CdS_{0.69}Se_{0.31}$ single crystal grown by sublimation method. Hall effect measurement were carried out by the Van der Pauw method. The measurement values under the temperature were found to be carrier density $n=1.95{\times}10^{23}m^{-3}$, Hall coeffcient $RH=3.21{\times}10^{-5}m^3/c$, conductivity ${\sigma}=362.41{\Omega}^{-1}m^{-1}$, and Hall mobility ${\mu}=1.16{\times}10^{-2}m^2/v.s.$ Heterojunction solar cells of $n-CdS_{0.69}Se_{0.31}/p-Cu_{2-x}S_{0.69}Se_{0.31}$ were fabricated by the substitution reaction. The open-circuit voltage, short-circuit currint density, fill factor and power conversion efficiency of $n-CdS_{0.69}Se_{0.31}/p-Cu_{2-x}S_{0.69}Se_{0.31}$ heterojunction solar cell under $80mW/cm^2$ illumination were found to be 0.41V, $19.5mA/cm^2$, 0.75 and 9.99%, respectivity.

  • PDF

Development of Dynamic Fiber Optic Gyrocompass (동적방식 광섬유자이로 콤파스의 제작)

  • Lee, Seok-Jeong;Choi, Woo-Jin;Bae, Jeong-Chul;Kim, Sung-Jin;Lee, Sang-Sik;Kwon, Yong-Soo;Hong, Tchang-Hee
    • Journal of the Korean Institute of Navigation
    • /
    • v.21 no.3
    • /
    • pp.67-74
    • /
    • 1997
  • This paper described the method and the result of making a dynamic fiber optic gyrocompass measuring the heading angles of ships by processing the output signal from a constant rotating fiber optic sensor and also showed the measurement to test the performance of our system. Considerig an economical view we designed and ordered a cheap medium grade fiber densors increased not fiber length but the diameter of a fiber sensing loop. The scale factor and noise was 267mV/deg/s and 2 deg/hr/$\sqrt{Hz}(1{\sigma})$, respectively. We made the dynamic fiber optic gyrocompass by this sensor. We measured the heading angles in an arbitrary direction to evaluate the accuracy of our system and the root mean square error was $0.4^\circ$. Moreover, we measured the angles ineach direction of $45^\circ$. successive rotation to know whether this system has distoritions in a specific direction or not and the root mean square error in this case was $0.5^\circ$.

  • PDF

Uncooled amorphous silicon 16x16 infrared focal plane arrays development (비정질 실리콘 기반의 비냉각형 16x16 적외선 초점면배열의 개발)

  • Cheon, Sang-Hoon;Cho, Seong-M.;Yang, Woo-Seok;Ryu, Ho-Jun;Yang, Ki-Dong;Yu, Byoung-Gon;Choi, Chang-Auck
    • Journal of Sensor Science and Technology
    • /
    • v.18 no.4
    • /
    • pp.301-306
    • /
    • 2009
  • This paper describes the design and fabrication of 16$\times$16 microbolometer infrared focal plane arrays based on iMEMS technology. Amorphous silicon was used for infrared-sensitive material, and it showed the resistance of 18 Mohm and the temperature coefficient of resistivity of -2.4 %. The fabricated sensors exhibited responsivity of 78 kV/W and thermal time constant of 8.0 msec at a bias voltage of 0.5 V. The array performances had satisfactory uniformity less than 5 % within one-sigma. Also, 1/f noise of pixel was measured and the noise factor of $6\times10^{-11}$ was extracted. Finally, we obtained detectivity of $1.27\times10^9cmHz^{0.5}/W$ and noise equivalent temperature difference of 200 mK at a frame rate of 30 Hz.

Regulation of the sufABCDSE Operon by Fur

  • Lee, Joon-Hee;Yeo, Won-Sik;Roe, Jung-Hye
    • Journal of Microbiology
    • /
    • v.41 no.2
    • /
    • pp.109-114
    • /
    • 2003
  • A promoter that is inducible by paraquat and menadione, the superoxide generators, independently of soxRS has been found in front of the sufABCDSE operon in Escherichia coli. Based on the observation that SufA is a holomog of IscA that functions in the assembly of iron sulfur cluster and the sufA promoter (sufAp) contains a putative Fur-binding consensus, we investigated whether this gene is regulated by Fur, a ferric uptake regulator, When examined in several sufAp-lacZ chromosomal fusion strains, sufAp was induced by EDTA, an iron chelator and a well-known Fur-inducer, The basal level of sufA expression increased dramatically in fur mutant, suggesting repression of sufAp by Fur. The derepression in fur mutant and EDTA-induction of sufA expression required nucleotides up to -61, where a putative Fur box is located. Purified Fur protein bound to the DNA fragment containing the putative Fur box between -35 and -10 promoter elements. The regulation by Fur and menadione induction of sufAp acted independently. The rpoS mutation increased sufA induction by menadione, suggesting that the stationary sigma factor RpoS acts negatively on sufA induction.

Enhancement of the Ultrasonic Image Using the Adaptive Window Log Filter for NDI of Aircraft Composite Materials (항공기 복합 재료의 비파괴 검사(NDI)를 위한 가변 창 필터를 이용한 초음파 영상 개선)

  • Hong, G.Y.;Hong, S.B.
    • Journal of the Korean Society for Aviation and Aeronautics
    • /
    • v.11 no.2
    • /
    • pp.33-42
    • /
    • 2003
  • In this paper, we propose an enhancement of the ultrasonic image for non-destructive inspection of aircraft composite materials. The ultrasonic images are corrupted by a speckle noise which has the characteristic of granular pattern noise and is in all types of coherent imaging systems, the signal independent and multiplicative noise. In this paper, we derive a filter, called the AWLF(Adaptive Window Log Filter), from the nature of the speckle. The filter is made of the MEAN Filter in the edge region and Log Filter in the flat or noise region. To make a distinction between edge and flat region, we calculate the inclination around the local window instead of computing the local statistics of pixels such as local mean ${\bar{M}}$ and standard deviation ${\sigma}_s$. According to the obtained region, edge region is performed by the mean filter and flat region by the Log filter. Performance of the proposed filter is evaluated by the Enhanced Factor$(F_e)$ and the Speckle Index(SI).

  • PDF