• 품질경영학회지
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• 제34권4호
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• pp.22-32
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• 2006

In these days, most of Korean industries advocates and utilizes Six Sigma as an integrated innovation tool for all their business and manufacturing processes. Six Sigma is known to bring more financial and managerial achievements than other innovation methods and tools. However, such successes are not always guaranteed. To adopt and implement Six Sigma successfully, it is necessary to grasp and understand essential characteristics of Six Sigma and then identify suitable elements and complements in consideration of vision, strategies, capability, circumstance and environment of the company. Step by step implementation plan may be built based on those ingredients. This study is to find out key ingredients (KIs) of Six Sigma. Potential ingredients selected from previous studies of success factors and ingredients of Six Sigma and TQM, and criteria of quality awards like MBNQA, EFQM, Deming prize and Korean National Quality Awards (KNQA) are classified into 11 categories based on experts' opinion and affinity analysis: management leadership, belt system, training, six sigma system, compensation, organization, corporate culture, customer focused, project selection, management of project results, and managerial achievements. Irrelevant or ineffective potential ingredients are discarded using factor analysis for the questionnaires answered by champions, MBB's and BB's of 90 Korean companies that have more than 3 years experience of Six Sigma. Also from the factor analysis, 3 factors are derived for each categories respectively except the managerial achievement category. Those 11 categories and 30 factors are defined as KIs of Six Sigma. This study also reveals that there is difference according to enterprise size, type of business, and implementation time in terms of KI's, and prioritizes KI's that an enterprise to pursue Six Sigma successfully should consider according to its characteristics.

• 미생물학회지
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• 제32권4호
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• pp.264-270
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• 1994

세균의 RNA 중합효소에서 여러 ${\sigma}$ 인자들 간에 보존된 아미노산 서열중 2.3 부위와 4.2 부위의 아미노산 서열로부터 유 n하여 두가지의 PCR primer를 제작하였다. 이들을 이용하여 PCR을 수행하였을 때, E. coli와 Streptomyces coelicolor의 DNA로부터 예상되었던 480 bp 정도의 DNA가 증폭되는 것을 관찰하였다. E. coli DNA에서 증폭된 DNA를 클로닝하여 염기서열을 결정한 결과 E. coli의 rpoS 유전자로부터 유래하였음을 알았다. 이를 탐침으로 S. coelicolor에서 genomic DNA hybridization을 수행하였을 때, PvuII 절편 두가지 (3.5 kb, 2.0 kb) 와 SalI 절편 두가지(3.4kb, 1.5 kb)에 탐침이 결합하는 것을 관찰하였다. 3.5 kb의 pvuII 절편을 sublibrary로부터 클로닝하고, 탐침이 결합하는 1.0kb의 BamHI/HincII 절편의 염기서열을 분석하였다. 부분적으로 결정된 염기서열을 BLAST 프로그램을 이용하여 GenBank와 EMBL, PDB 등의 data library의 유전자들과 비교하여 본 결과Streptomyces속의 ${\sigma}$인자들을 비롯한 Synechococcus종, Anabaena종, Pseudomonas aeruginosa, Stigmatella aurantica 등의 주된 ${\sigma}$ 인자와 높은 유사성을 보였다. 현재까지 1.2 부위와 4 부위에 해당하는 부분의 염기서열을 결정하였는데, 이 부분은 S. coelicolor에서 알려진 다섯가지의 ${\sigma}$ 인자 유전자 중 hrdA와 가장 높은 유사성을 보이며, 아미노산의 유사성이 1.2부위에서는 88%, 4 부위에서는 75%인 것으로 나타났다.

• 대한산업공학회지
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• 제32권4호
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• pp.279-290
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• 2006

This paper proposes a model to assess the maturity level of Six Sigma activities. We classify the maturity level into 5 stages: initial, forming, storming, performing and mature stage. To evaluate the maturity level, 10 categories of Six Sigma with 3 factors each are identified: management leadership, belt system, expert training, establishing execution system, compensation, organization, corporate culture, customer focus, project selection, and management of project results. Scoring 277 items in total, the value of each factor is evaluated by weighted average of those items. Maturity level is appraised by rating the sum of scores of 10 categories that are obtained by summing up the values of its 3 factors. Values of weights and criteria of rating maturity levels are determined by analyzing 90 companies and Six Sigma exper's opinion. This study also shows the actual appraisal results of some companies.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2001년도 학술 발표회 진행표 및 논문초록
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• pp.63-63
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• 2001

SigR ($\sigma$$\^$R/) is a sigma factor responsible for inducing the thioredoxin system in response to oxidative stress in Streptomyces coelicolor. RsrA specifically binds to $\sigma$$\^$R/ and inhibits $\sigma$$\^$R/-directed transcription under reducing conditions. Exposure to H$_2$O$_2$ or thiol-specific oxidant diamide dissociates $\sigma$$\^$R/-RsrA complex. RsrA contains 7 cysteine residues in 105 total amino acid residues.(omitted)

• Journal of Microbiology
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• 제45권6호
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• pp.534-540
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• 2007

The Streptomyces coelicolor A3(2) genome contains six operons (rrnA to F) for ribosomal RNA synthesis. Transcription from rrnD occurs from four promoters (p1 to p4). We found that transcripts from the p1 and p3 promoters were most abundant in vivo in the early exponential phase. However, at later phases of exponential and stationary growth, transcripts from the p1 promoter decreased drastically, with the p3 and p4 transcripts constituting the major forms. Partially purified RNA polymerase supported transcription from the p3 and p4 promoters, whereas pure reconstituted RNA polymerase with core enzyme (E) and the major vegetative sigma factor ${\sigma}^{HrdB}$ ($E{\cdot}{\sigma}^{HrdB}$) did not. In order to assess any potential requirement for additional factor(s) that allow transcription from the p3 and p4 promoters, we fractionated a partially purified RNA polymerase preparation by denaturing gel filtration chromatography. We found that transcription from the p3 and p4 promoters required factor(s) of about 30-35 kDa in addition to RNAP holoenzyme ($E{\cdot}{\sigma}^{HrdB}$). Therefore, transcription from the p3 and p4 promoters, which contain a consensus -10 region but no -35 for ${\sigma}^{HrdB}$ recognition, are likely to be regulated by transcription factor(s) that modulate RNA polymerase holoenzyme activity in S. coelicolor.

• 한국품질경영학회:학술대회논문집
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• 한국품질경영학회 2004년도 품질경영모델을 통한 가치 창출
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• pp.217-228
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• 2004

In this thesis, we suggest questionnaire which is designed to understand of what employees include executive think about 6 sigma management movement and to find important factor for strategic of success 6 sigma management movement of K. Corporation. This thesis is composed of 2 parts, one is questionnaire design and the other is questionnaire analysis. We find important factor for strategic of success 6 sigma management movement of K. Corporation by using correlation and regression analysis, which results very useful and great help for K's Co. In questionnaire analysis, we use by minitab package version 13.2.

• 한국식품과학회지
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• 제36권3호
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• pp.456-460
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• 2004

L. monocytogenes가 biofilm을 생성하는데 ${\sigma}^{B}$가 어떤 영향을 미치는가를 구명하기 위해 L monocytogenes wild type인 10403S와 ${\sigma}^{B}$를 제거한 sigB null mutant의 biofilm 생성능을 고삼투압 및 저온 조건에서 비교하였다. 고삼투압 조건인 6%의 NaCl이 첨가된 BHI 배지에서 배양된 L. monocytogenes 10403S는 배양 72시간 후 $6.83{\pm}0.38\;log\;cfu/cm^{2}$의 biofilm을 생성하였으며, sigB null mutant의 경우는 $5.33{\pm}0.45log\;cfu/cm^{2}$ 의 biofilm을 생성하였는데, L. monocytogenes 10403S가 sigB null mutant보다 31.8배나 많은 biofilm을 생성하였다. 또한 L. monocytogenes 10403S를 6%의 NaCl이 첨가된 BHI 배지에서 배양했을 시 NaCl을 첨가하지 않은 배지에서 배양한 경우보다 4.7배나 많은 biofilm을 생성하였는데, L. monocytogenes 10403S와 같이 ${\sigma}^{B}$가 존재하는 경우 고삼투압 조건에서 biofilm을 더욱 많이 생성하였으며, ${\sigma}^{B}$가 biofilm의 생성에 영향을 미친다고 할 수 있었다. 또한 저온 조건($4^{\circ}C$ 배양)에서 ${\sigma}^{B}$가 biofilm 생성에 영향을 미치는지를 조사하였는데, ${\sigma}^{B}$는 저온 스트레스 시 biofilm 생성에 영향을 미치지 않는 것으로 나타났다.

• 품질경영학회지
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• 제31권1호
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• pp.123-131
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• 2003

This paper considers a six sigma project for reducing the defects rate of the welding process in manufacturing firms. The project follows a disciplined process of five macro phases. define, measure, analyze, improve and control(DMAIC). The need of customers is used to identify critical to quality(CTQ) of project. And a process map is used to identify process input factors of CTQ. Four key process input factors are selected by using an input factor evaluation of teams; an interval of welding, an abrasion, an electric current and a moving freely. DOE is utilized for finding the optimal process conditions of the three key process input factors. Another one key input factor improved to welding machine. The six sigma level of defects rate becomes a 2.01 from a 1.61 at the beginning of the project.

• 한국품질경영학회:학술대회논문집
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• 한국품질경영학회 2006년도 추계 학술대회
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• pp.174-180
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• 2006

This study to find out key ingredients(KIs) of Six Sigma. Potential ingredients are selected from previous studies of success factors and ingredients of Six Sigma and TQM, and criteria of quality awards like MBNQA, EFQM, Deming and Korean National Quality Award(KNQA) are classified into 11 categories based on experts' opinion and affinity analysis. Irrelevant or ineffective potential ingredients are discarded by using factor analysis for the questionnaires answered by champions, MBBs and BBs of 90 Korean companies that have more than 3 years of experience in implementing Six Sigma. In addition, from the factor analysis, 3 factors are derived for each respectively except the managerial achievement category. Those 11 categories and 30 factors are defined as Six Sigma KIs.

• 산업경영시스템학회지
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• 제30권4호
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• pp.78-84
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• 2007

After 6 Sigma administration is introduced in domestic in 1997, many corporations are utilizing by survival strategy and method of administration reform. This is applied in all field that this led to R&D from service industry by the next. Success factors by each field was studied and announced applying in various types of business. The Korail and Korea Post, the Ministry of Environment and various public corporation and governmen and municipal office are introduce and utilizing 6 Sigma administration by innovation method present. But, research about success factor that special quality of public sector is considered up to now was almost no. This treatise analyzed 6 Sigma' success factors and effect relation to public corporation.