• Title/Summary/Keyword: sigH

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Bacterial Traits Involved in Colonization of Arabidopsis thaliana Roots by Bacillus amyloliquefaciens FZB42

  • Dietel, Kristin;Beator, Barbara;Budiharjo, Anto;Fan, Ben;Borriss, Rainer
    • The Plant Pathology Journal
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    • v.29 no.1
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    • pp.59-66
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    • 2013
  • Colonization studies previously performed with a green-fluorescent-protein, GFP, labeled derivative of Bacillus amyloliquefaciens FZB42 revealed that the bacterium behaved different in colonizing surfaces of plant roots of different species (Fan et al., 2012). In order to extend these studies and to elucidate which genes are crucial for root colonization, we applied targeted mutant strains to Arabidopsis seedlings. The fates of root colonization in mutant strains impaired in synthesis of alternative sigma factors, non-ribosomal synthesis of lipopeptides and polyketides, biofilm formation, swarming motility, and plant growth promoting activity were analyzed by confocal laser scanning microscopy. Whilst the wild-type strain heavily colonized surfaces of root tips and lateral roots, the mutant strains were impaired in their ability to colonize root tips and most of them were unable to colonize lateral roots. Ability to colonize plant roots is not only dependent on the ability to form biofilms or swarming motility. Six mutants, deficient in abrB-, sigH-, sigD-, nrfA-, yusV and RBAM017410, but not affected in biofilm formation, displayed significantly reduced root colonization. The nrfA- and yusV-mutant strains colonized border cells and, partly, root surfaces but did not colonize root tips or lateral roots.

A VLSI Efficient Design and Implementation of Bit Plane Coding Algorithm for JPEG2000 (JPEG2000을 위한 Bit Plane Coding Algorithm의 효율적인 VLSI 설계 및 구현)

  • Yang, Sang-Hoon;Min, Byung-Jun;Park, Dong-Sun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.1
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    • pp.146-150
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    • 2009
  • Nowdays needs the new still image compression standard. JPEG2000 has been developed. JPEG2000 divide DWT and EBCOT. EBCOT is consisted of Bit Plane Coding and ARithmetic Coding algorithm. In this paper, we proposed BPC algorithm that is efficient context-based generation. Proposed BPC Algorithm forecasted coding pass using SigStage, column, mpass value. BPC designed using Verilog HDL. H/W implemenates using Xillinx FPGA technology.

Study of Methane Storage through Structure Transition of Gas Hydrate (가스하이드레이트 구조 변형을 통한 메탄 저장에 관한 연구)

  • Lee, Ju-Dong;Lee, Man-Sig;Kim, Young-Seok
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.11a
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    • pp.54-57
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    • 2006
  • Structure H formation experiments were conducted in a semi-batch stirred vessel using methane as the small guest substance and neohexane(NH), tert-butylmethylether(TBME) and methylcyclohexane(MCH) as the large molecule guest substance (LMGS). The results indicate that the rates of gas uptake and induction times are generally dependent on the magnitude of the driving force. When tert-butyl methyl ether is used as the LMGS rapid hydrate formation, much smaller induct ion time and rapid decomposition can be achieved. Liquid-liquid equilibrium (LLE) data for the above LMGS with water have been measured under atmospheric pressure at 275.5, 283.15K, and 298.15K. It was found that TBME is the most water soluble followed by NM and MCH. The solubility of water in the non-aqueous liquid was found to increase in the following order: MCH

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Effects of Asymmetric Airway Inertance on Mean Lung Volume During High Frequency Ventilation(HFV)

  • Cha, Eun-Jong
    • Journal of Biomedical Engineering Research
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    • v.11 no.2
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    • pp.203-208
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    • 1990
  • A possible asymmetry in airway inertance was modeled based on previously reported radiographic visualization data of the airway wall fluctuation in intact dogs. Effects of asymmetric Inertance on mean lung volume during high frequency ventilation (HFV) were evaluated in terms of mean inertive pressure drop across the airways. It was found that a negligible inertlve pressure drop was expected ($<1{\;}cmH_20$) in normal subjects, while a sig- nificant rise in mean alveolar pressure compared to mean mouth pressure by approximately $3{\;}cmH_20$ was resulted for about 40% airway fluctuation representing bronchoconstriction state by Histamine induction. These results demonstrate that asymmetric Inertance could lead patients with airway diseases to a significant lung hyperinflation (LHI), and bronchodilation treatment is recommended prior to applying HFV to prevent those patients from a possible barotrauma.

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The Crystal and Molecular Structure of Niclosamide monohydrate, $C_{13}H_8Cl_2N_2O_4\cdotH_2O$ (Niclosamide monohydrate, $C_{13}H_8Cl_2N_2O_4\cdotH_2O$의 결정 및 분자구조)

  • 김의성;신현소
    • Korean Journal of Crystallography
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    • v.4 no.1
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    • pp.1-5
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    • 1993
  • The crystal structure of N-(2'-chloro-4'-vitrophenyl)-5-chlorosalicylamide( Niclosamide ) monohydrate has been determined from 1976 sig- nificant independent reflections collected on an automated CAD4 diffractometer using graphitemonochromated Mo-Ka radiation. The crystal is monoclinic, space group P211c, with unit cell dimensions, a=11.331 (3), b=16.964(2), c=7.347(4)A , P =98.20(3)° and Z=4 at T=293k. The structure was solved by direct method using seminvariants of ggg Parity group and refined by the full-matrix least-square method, resulting model with reliability factor, R=0.046. The feature of the molecule show planar structure in parallel to the ab crystal plane.

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A Study of Hydroxyapatite Production from Waste Oyster Used Mechanochemical Treatment

  • Kim, Sun-Tae;Oh, Chi-Jung;Kim, Wan-Tae;Kim, Young-Sig;Kim, Myong-Jun
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.469-471
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    • 2001
  • Dry grinding of a mixture of CaCO$_3$ and Ca(H$_2$PO$_4$)$_2$.$H_2O$ was conducted using a planetary ball mill in order to investigate solid state reaction for a synthesis of hydroxyapatite(Ca$_{10}$(PO$_4$)$_{6}$(OH)$_2$, HAp) through mechanochemical treatment method. The raw materials, which are composed of waste oyster and calcium biphosphate Ca(H$_2$PO$_4$)$_2$.$H_2O$, were mixed and then treated mechanochemically. The synthesis of hydroxyapatite(Ca$_{10}$(PO$_4$)$_{6}$(OH)$_2$, HAp) from the mixture was almost completed by about 60 minute grinding. The formation of HAp monophase in the ground mixture was characterized through X-ray diffraction (XRD) analysis. Moreover, the formation of HAp monophase depending on the grinding time was improved by increasing the grinding time.ime.ime.

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Detect of Hypericin (HyH) gene in Hypericum erectum in Korea and Comparison of H. perforatum in Europe (한국내 고추나물의 하이퍼리신 유전자(HyH)의 탐색과 유럽의 서양고추나물과 비교)

  • Huh, Man-Kyu
    • Journal of Life Science
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    • v.17 no.8 s.88
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    • pp.1034-1038
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    • 2007
  • Hypericin (HyH) is a substance which is isolated a medicinal herb, Hypericum perforatum L., commonly known as St. John's Wort. Hypericum erectum is a long-lived herb that is distributed in Korea. Cloned HyH genes H. erectum of were conformed by sequencing. The cDNA Hyp-1 sequence has 732 bp with an open reading frame of 567. Thus coding for a protein of 152 amino acid residues. A BLAST re-search using the deduced nucleotide sequences in HyH gene produced significant alignments with the H. perforatum. Sequences in HyH gene showed significant homology with Rubus idaeus putative allergen Rub-i-1 mRNA, Protein sequence comparisons revealed significant homology between Hyp-1 and the phenolic oxidative coupling protein hyp-1 of H. perforatum (98%). Additionally, Hyp-1 showed sig-nificant homology with various other classes of allergens, including Pru-av-1 (62%) from Prunus avium and allergen Bet-vl-Sc3 from Betula pendula (60%). Thus, the result of this study may offer an important information to establish an assay system for chemicals of the herbal medicines for H. erectum as well as H. perforatum.

Influence of solvent on the nano porous silica aerogels prepared by ambient drying process (상압건조 나노다공성 실리카 에어로젤에 대한 용매의 영향)

  • Ryu, Sung-Wuk;Kim, Sang-Sig;Oh, Young-Jei
    • Journal of Sensor Science and Technology
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    • v.15 no.5
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    • pp.371-377
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    • 2006
  • Nano porous, transparent silica aerogels monoliths were prepared under ambient drying (1 atm, $270^{\circ}C$) condition by the combination of sol-gel process and surface modification with subsequent heat treatment. Three kinds of solvent, n-hexane, n-heptane and xylene, were selected in the point view of low surface tension and vapor pressure in order to restrain a formation of cracks during drying. Crack-free silica aerogels with over 93 % of porosity and below $0.14g/cm^3$ of density were obtained by solvent exchange and surface modification under atmosphere condition. Optimum solvent was confirmed n-heptane among these solvents through estimation of FT-IR, TGA, BET and SEM. Modified silica aerogel exhibited a higher porosity and pore size compare to unmodified aerogels. Hydrophobicity was also controled by C-H and H-OH bonding state in the gel structure and heat treatment over $400^{\circ}C$ effects to the hydrophobicity due to oxidation of C-H radicals.

Hematological and Serum Chemical Findings following Repeated Medication of Diminazene Aceturate in Canine Babesiosis (만성 Babesia 감염견에서의 Diminazene Aceturate의 반복투여에 따른 혈액학 및 혈액화학적 변화)

  • 황미정;이희석;이근우
    • Journal of Veterinary Clinics
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    • v.17 no.2
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    • pp.349-358
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    • 2000
  • Effects of repeated administration of diminazene aceturate (Hoechst Veterin r GmbH. Ger- many) that has been introduced as effective compound against Babesiosis and trppanosomiasis were investigated in dogs experimentally infacted with Babesia gibsoni. Adull mongrel dogs of both sexes were inoculated will nonpreserved infected blood and then maintained chronic anemia. A single dose of diminazene aceturate of 7mg/kg b.w. was administrated intramnuscularIy on day 7.1 arid ,7. Clin- ical and hcmatological findings following inoculation and medication were observed and 7enlm bio chemical analysis also was monitored. Parasitemia was detected between 3 and 6 days after inoculation. The rate of parasitized erythrocytes,1 in peripheral blood reached the peak on the 13th day and was maintained the percentage of 0.1 to 1.0 until the medication of diminazene aceturate. RBC was significantly (p<0.01) decreased on the 3rd day and then kept on decreasing. The lowest value was observed on the 16th day. WBC remained generally within normal ranges. PCV revea1ed the sig-nificant (p<0.01) decrease within the range of 24-27% and platelet was significantly (p<0.05) decreased during the period. Senum chemical values (ALT, AST. total bilirubin. LDH BUN, area- tinine, total protein. albumin and glucose) were within normal ranges during the experimental period. Serum CPK values were significantly (p<0.01) increased on the 3rd day. There was no clinically, sig-nificant difference in a single dose of diminazene aceturate of 7 mg/kg b.w. But the administration of diminazene aceturate of 14 mg/kg b.w. revealed vomiting and anorexia and one dog died in 30 hours after administration. The administration of 14mg/kg b.w. resulted in vomiting, salivation, actor- exia, tremor of head and involuntary movement and one dog died in 27 hours after administration. WBC, RBC, PCV and Platelet values were no significant difference and hematological findings revealed persistent anemia and thrombocytopenia during chronic anemia after inoculation. AST activity its was significantly (p<0.01) increased 11\\`from 3 days after medication and AST activity was on the same trend. Serum CPK activity revealed significant (p<0.01) increase within 6 hors)\\`s after every administration and decreased in 48 howl·s after administration.

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Isolation and Characterization of an Alkaline Cellulase Produced by Alkalophilic Bacillus sp. HSH-810 (알칼리성 Cellulase를 생산하는 호알칼리성 Bacillus sp. HSH-810의 분리 및 효소 특성)

  • 김지연;허성호;홍정화
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.139-146
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    • 2004
  • A bacterium producing alkaline cellulase was isolated from soil, leaf mold and compost, and was identified as alkalophilic Bacillus sp. HSH-810 by morphological, cultural and biochemical determination. The optimum cul-ture condition of Bacillus sp. HSH-810 for the growth and alkaline cellulase production was $30^{\circ}C$ and pH 10.0. The maximum alkaline cellulase production was obtained when 1.0%(w/v) CMC, 0.5%(w/v) peptone, 0.02%(w/v) $CaCl_2$ and 0.02(w/v) $CoCl_2$ were used as carbon source, nitrogen source and mineral source, respectively. The optimum pH and temperature of the enzyme activity were pH 10.5 and $50^{\circ}C$, respectively. This enzyme was fairly stable in the pH range of 6.0-13.0 and at $50^{\circ}C$. For the effect of surfactants, the activity of alkaline cellulase was stable in the presence of sodium-$\alpha$-olefin sulfonate (AOS), sodium dodecyl sulfonate (SDS), Tween 20 and Tween 80, but inhibited by the presence of 0.1 linear alkyl-benzene sulfonate (LAS) sig-nificantly.