• Mycobiology
• /
• 제35권1호
• /
• pp.30-35
• /
• 2007

The fruits showing brown rot symptom on dwarf flowering almond were found in Gongju, Chungchungnam-Do in Korea in July 2005. Small water-soaked lesions on the fruits were initiated, and gradually developed to soft rot covered with gray conidia. Then the diseased fruits were shrunk and became grayish-black mummies. A fungus was isolated from the diseased fruit and its morphological, cultural and molecular genetic characteristics were investigated. Typical blastospores of Monilinia spp. were observed under a light microscope both from tissues of the diseased fruits and from PDA-grown cultures. The fungus grew well at $25^{\circ}C$ and on PDA. The ITS ribosomal DNA region (650 bp) of the fungus was amplified by PCR and analyzed. Comparative data on ITS sequence homology among Monilinia spp., ITS sequence-based phylogram and morphological characteristics showed that the fungus is Monilinia fructicola. This is the first report on Monilinia fructicola causing brown rot on fruits of dwarf flowering almond in Korea.

• 한국균학회지
• /
• 제51권1호
• /
• pp.51-56
• /
• 2023

황칠나무는 두릅나무과에 속하는 난대성 상록활엽수로 우리나라 제주도, 보길도, 거문도, 거제도, 완도, 해남 등에 분포한다. 2021년 6월 전라남도 나주에서 황칠나무 유묘의 지제부가 갈변하고 잘록해지는 뿌리썩음병을 국내 최초로 발견하였다. 황칠나무 뿌리썩음병균을 분리 및 동정하기 위해, 유묘의 병든 조직에서 3개의 균주를 분리하였고, PDA 배지에서 형태적, 균학적 특성 조사한 결과 Fusarium oxysporum으로 동정되었다. Internal transcribed spacer (ITS) 및 translation elongation factor 1-α (EF1-α) 염기서열을 통한 분자계통학적 분석을 수행하여 한국의 황칠나무 뿌리썩음병균은 F. oxysporum임을 확인하였다. 병원성 테스트를 위해 유묘의 뿌리를 분생포자 현탁액에 침지하여 정식하였고, 접종 20일 후 처리구에서 뿌리썩음 및 지제부의 갈변 증상을 확인하였다. 본 연구는 한국의 황칠나무 유묘에서 뿌리썩음병균 F. oxysporum의 첫 보고이다.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2003년도 춘계학술대회논문집
• /
• pp.319-324
• /
• 2003

In general industrial rotating machinery is operated under 3,600 rpm as rotating speed and designed to have critical speed that is above operating speed. So, there was no problem to operate rotating machine under critical speed. But nowadays, they should be operated more than the frist critical speed as usual with the trend of high speed, large scale and hish precision in industries. In case of the large rotor assembly as the trend of large scale, using fitting method of disk or cylinder on shaft is rising for the convenience of assembly and cutting down of manufacturing cost. The shrink fitting is used to assemble lamination part on shaft for manufacturing of rotor of motor or generator in many cases and also is widely used for other machinery. In rotating system, which is compose of rotor and bearing, the critical speed is determined from inertia and stiffness for the rotor and bearings. In case of fitting assembly, analysis and design of the rotor is not easy because the rotor stiffness is determined depend on a lot of factors such as shaft material/dimension, disk material/dimension and assembled interference etc. Therefore designer who makes a plan for hish-speed rotating machine should design that the critical speed is located out of operating range, as dangerous factors exist in it. In order to appropriate design, an accurate estimation of stiffness and damping is very important. The stiffness variation depend on fitting interference is a factor that changes critical speed and if it's possible to estimate it, that Is very useful to design rotor-bearing system. In this paper, the natural frequency variation of the rotor depends on fitting interference between basic shaft and cylinder is examined by experimentation. From the result, their correlation is evaluated quantitatively using numerical analysis that is introduced equivalent diameter end the calculation criteria is presented for designer who design fitting assembly to apply with ease for determination of appropriate interference.

• 한국버섯학회지
• /
• 제9권4호
• /
• pp.198-201
• /
• 2011

Cobweb disease symptoms were observed in a mushroom farm in Buye, Korea during a disease survey in 2008-2011. Five isolates of Cladobotryum sp. were obtained from the infected caps and stipes. These isolates of Cladobotryum sp. were identified as C. mycophilum based on their morphological, cultural characteristics and analysis of the ITS sequences. Early symptoms were noticed as round, fleshy, yellowish brown lesions on mushroom caps. Late symptoms progressed when the parasitic fungus formed white cobweb circular colonies on dead or damaged pinheads, spread on the surface of the casing, and covered entirely fruiting bodies. Optimal temperature and pH for mycelial growth on MEA is $23^{\circ}C$ and 6.0. Microscopically the spores of the fungus are large and most 2~3 celled produced on vertically branched conidiophores. Mushroom caps turned dark brown and shrunk due to soft rot. Testing of sensitivity to selected fungicides showed that isolate was highly resistance to Mancozeb and Thiophanate-methyl, moderately sensitivity to Iprodione, and highly sensitivity to Benomyl, Prochloraz-Mn and Carbendazim.