• Asian-Australasian Journal of Animal Sciences
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• v.26 no.8
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• pp.1144-1151
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• 2013

In this study, protein domains with cellulase activity in goat rumen microbes were investigated using metagenomic and bioinformatic analyses. After the complete genome of goat rumen microbes was obtained using a shotgun sequencing method, 217,892,109 pair reads were filtered, including only those with 70% identity, 100-bp matches, and thresholds below $E^{-10}$ using METAIDBA. These filtered contigs were assembled and annotated using blastN against the NCBI nucleotide database. As a result, a microbial community structure with 1431 species was analyzed, among which Prevotella ruminicola 23 bacteria and Butyrivibrio proteoclasticus B316 were the dominant groups. In parallel, 201 sequences related with cellulase activities (EC.3.2.1.4) were obtained through blast searches using the enzyme.dat file provided by the NCBI database. After translating the nucleotide sequence into a protein sequence using Interproscan, 28 protein domains with cellulase activity were identified using the HMMER package with threshold E values below $10^{-5}$. Cellulase activity protein domain profiling showed that the major protein domains such as lipase GDSL, cellulase, and Glyco hydro 10 were present in bacterial species with strong cellulase activities. Furthermore, correlation plots clearly displayed the strong positive correlation between some protein domain groups, which was indicative of microbial adaption in the goat rumen based on feeding habits. This is the first metagenomic analysis of cellulase activity protein domains using bioinformatics from the goat rumen.

• Clean Technology
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• v.4 no.2
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• pp.41-53
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• 1998

Most of flame furnace with uncontrolled atmosphere forms oxidation scales in the surface during heat treatment. In this case, shot blasting and pickling are commonly used for reduction of oxidation scales. These processes have many disadvantages, such as long process time, high operating cost and environmental problems due to polishing dust, etc. Hence, this study aimed to develop the advanced heat treating equipment which could make a controlled atmosphere using a heatresisting structure into the flame furnace. The analysis result with SEM, EPMA, and EDX revealed that the amount of scales formed is much less than that with the conventional flame furnace. A brightness of the sample treated with the advanced process is similar compared to the treatment with vacuum furnace. In the present work, it was recommended that the inside structure of the furnace and gas line system could made up of SBB410 and STS310, respectively. The operating cost with the advanced heat treatment system could be reduced annually by forty million won. As a result of this study, it may be possible to reduce the oxidation scales. The fundamental information obtained in this study will be useful not only for improving the heat treatment process(reduction of shot blast and cleaning process), but for promoting the manufacture of bright products.

• Journal of Korean Society of Steel Construction
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• v.12 no.2 s.45
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• pp.167-175
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• 2000

An experimental research to evaluate the slip coefficient of high-strength slip-critical-type bolt connection of weathering steel plate has been performed in this paper. Experiments were performed with several surface conditions such as clean mill scale with hand or power brush cleaning, shot blast cleaned, inorganic zinc primer coated, and weather coated surfaces. Also, the relaxation of bolt clamping force was estimated during 500 hours. It was ascertained from the experiments that slip coefficients are greater than 0.40 in all faying surface conditions except mill scale surface with power brushing. The quantify of relaxation depended on the surface conditions and was $3{\sim}8%$, i.e., less than 10%.

• Journal of Korean Society of Steel Construction
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• v.12 no.2 s.45
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• pp.177-185
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• 2000

An experimental research to evaluate the slip coefficients of high-strength friction-type bolt connection of weathering steel plate has been performed in this paper. The test specimens with mill scale or shot blast had been exposed in open air during 3 and 6 months and cleaning of rust surface by hand brushing, power tool brushing and no cleaning was considered. The relaxation of bolt clamping force had also been measured during 600 hours. It was found that slip coefficients increased to the value over 0.6 with exposure except mill scale surface by power tool brushing. The relaxation of bolt tension force in exposed specimens also increased and maximum value reached to about 10%.

• Journal of the Korean Society for Precision Engineering
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• v.12 no.5
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• pp.69-76
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• 1995

This paper describes characteristics of EDT(Electro Discharge Texturing) and that the strip surface has influence on the paintability. The pulse mode and the capacitor mode of recently developed EDT method were investigated about the peak shape and the roughness of strip surface. And EDT method compared the above-mentioned contents with the SBT(Shot Blast Texturing) method. The peak count of the pulse mode is more than that of the capacitor mode. But the lower roughness below the Ra 3.2 .mu. m of the strip surface, the texturing time of the pulse mode is longer than the capacitor mode. In SBT method, the peak shape is similar to capacitor mode. But the EDT roll has more uniformity than the SBT roll and has more peak count than that of SBT roll. Therefore, in the painted steel sheet, EDT roll gives better surface of clarity image than the SBT roll.

• Journal of the Korea Institute of Building Construction
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• v.16 no.4
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• pp.341-348
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• 2016

The aim of this study is to suggest design requirements on the military barriers which are installed to reduce critical damage on protective facilities against sudden pin-point attacks caused by North Korean artilleries. For this purpose, site investigation and review of design drawings associated with barriers built in the contact areas are conducted. With identified data concerning barriers, the geometric modeling, which is used in the structural analysis, is performed. And then, the possible threat of North Korea is determined based on intelligence preparation of battlefields. Once the structural modeling and threat analysis are completed, structural damage on barriers and protective facilities are assessed in terms of impact, penetration, scabbing, and blast pressure effects. According to the analysis results, the thickness of barriers should be 450mm at least and current established barriers need to be structurally reinforced via sectional enlargement.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.15 no.2
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• pp.90-103
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• 2005

Objective: This study was carried out to assess the status of working environments, to improve the working environments, and to be helpful to prevent occupational disease. Methods: The authors measured the noise level, concentration of dust, heavy metals, organic solvents, and other chemicals at 95 industries (22 working processes) by type of working process in manufacturing industry of parts and accessories for motor vehicles and its engines from January to December 2003. Samples were measured and analyzed by regulations of Korea Ministry of Labor, manuals of National Institute for Occupational Safety and Health (NIOSH) and Occupational Safety and Health Administration (OSHA). Results: Results are as follows. 1. Major processes exceeding mean noise levels provided the American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value (TLV) were shakeout [97.5 dB(A)]. shot-blast [94.2 dB(A)], pressing [92.9 dB(A)], crushing [91.2 dB(A)], and cleaning [90.6 dB(A)]. 2. Mean concentrations of dusts were not exceeded to the TLV. But concentration on some points of processes as like welding (6.50 mg/m3), foundry (5.24 mg/m3) were exceeded. 3. Mean concentrations of heavy metals were not exceeded to the TLV. 4. Mean concentrations of organic solvents and chemicals were not exceeded to the TLV. 5. Improving rate for working environment were significantly higher in industries with health manager than without (p<0.05), and by increasing the year of working environmental measurement (p<0.01). Conclusions: The results suggest that working processes of exceeding to the TLV will be needed rapid improvement of the working environment, and also the others will be needed positive management of the working environment. Health managers must be recommended to employ in the workplace, and further studies for relationship between working environment and health effects for the workers must be carried out.