• The Journal of the Convergence on Culture Technology
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• v.4 no.4
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• pp.331-335
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• 2018

Zantedeschia spp. calla is very popular as a cut flower. It is very important to establish a micro propagation system through plant tissue culture with the problem that colored calla with various colors are low in natural reproduction rate and vulnerable to high temperature. In this study, we conducted the experiment by adding taurine to improve the growth of calla plant. When 20 mg/L of taurine was added with plant growth effect, 54.0 % of the cases of multiple shoots and 17.2 times of fresh weight were the most effective. Taurine 20 mg/L treatment showed 16.0 % and 39.2 %, respectively, than the untreated control. Taurine may contribute to mass propagation of elite breeding lines as well as an improvement of farm income by positively influencing the overall growth of calla plants, thereby positively affecting the establishment of the micro propagation system of calla shoot tips.

• Korean Journal of Environmental Agriculture
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• v.24 no.1
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• pp.66-70
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• 2005

The experiment was carried out to investigate the effects of gamma radiation on the early growth performance and physiological traits of BT2 clone tea, the most promising cultivar released by Bangladesh Tea Research Institute. The fresh shoot cuttings were irradiated with seven different levels of gamma radiation such as 0, 10, 20, 30, 40, 50 and 60 Gy from Cobalt 60Co source (Dept. of PlantBreeding, Bangladesh Institute of Nuclear Agriculture). Thereafter, the irradiated shoot cuttings were planted in polythene bags and kept under natural conditions. It was observed that callusing was initiated from 8th weeks after placement of tea shoot cuttings in the polythene bags and completed by 12th weeks. The morphological growth of tea shoot cuttings were recorded under varying levels of gamma radiation and growth stages. It was observed that the number of leaves, number of primary branches, base diameter, root length and total leaf area per plant significantly increased with the progress of time and increasing levels of gamma radiation, however, the plant height showed decreasing trend with the increasing levels of gamma radiation, which could be due to the change in chromosomal structure and genetic makeup. After 56 weeks of planting, the plant height, the number of leaves and primary branches per plant, base diameter, root length and total leaf area per plant recorded were 65.70 cm, 30.67, 7.33, 1.48 cm, 23.50 cm, and 1250.67 cm2 per plant respectively under the radiation level 60 Gy, whereas the corresponding figures of the above parameters at the control treatment were 76.21 cm, 18.33, 3.67, 0.92 cm, 17.75 cm and 778.33 cm2 per plant, respectively. A significant relationship was observed among the physiological growth parameters with the increasing levels of gamma radiation. The total dry matter gain, leaf area index, absolute growth rate and relative growth rate were significantly influenced with the rising levels of gamma radiation (up to 60 Gy), whereas the net assimilation rate of individual tea plant non-significantly responded as compared to those of control treatment. Finally after 56 weeks of planting, the maximum total dry weight gain, leaf area index, absolute growth rate, relative growth rate and net assimilation rate recorded under 60 Gay radiation level were 40.25 g/plant/week, 4.25, 1.18 g/week, 0.0621g/g/week and 17.07 g/m2/week respectively.

• Journal of Plant Biotechnology
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• v.32 no.4
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• pp.287-292
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• 2005

Calli were induced by culturing the leaf segment of Actinidia deliciosa ${\times}$ A. arguta clone 118 on MS medium supplemented with 0.5 mg/L 2,4-D, 0.1 mg/L NAA and 0.05 mg/L BA for 8 weeks in light condition. The induced calli were inoculated in liquid MS medium containing 0.5 mg/L 2,4-D, 0.1 mg/L NAA, 0.05 mg/L BA and 3% sucrose to establish cell suspension culture. The cells at the exponential stage and the stationary stage could be observed between 5-11 days and after that 12 days in culture, respectively. The fresh weight of callus induced from the suspended cells did not vary much among the media containing eight different combinations of plant growth regulators tested. The highest frequency of shoot induction (88.3%) was observed in MS medium containing 2.0 mg/L zeatin. Either BA or zeatin mixed with thidiazuron (TDZ) seemed to be effective in shoot induction. The induced shoots were transferred to MS medium containing 0.2 mg/L zeatin for further shoot growth. And then the shoots were transferred to Standardi (ST) medium containing 1.0 mg/L indolebutyric acid (IBA) for rooting. Plantlets could be obtained through cell suspension culture of Actinidia deliciosa ${\times}$ A. arguta clone 118.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.613-621
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• 2011

Plastic house and laboratory experiments were conducted to determine the effects of substrate components and shade degrees on plant growth and physiologically-active substance content and antioxidant activity of Panax ginseng. No significant difference in growth characteristics among substrates was observed. However, shade treatment also significantly reduced leaf area, chlorophyll content, root length, and shoot and root fresh weight (P<0.05) with increasing of the degree. Total phenolics level [mg ferulic acid equivalents (FAE) $kg^{-1}$ DW] was higher in root parts (25.8 to 28.4 mg $kg^{-1}$) than shoot parts (20.7 to 23.8 mg $kg^{-1}$) of Panax ginseng, and with shade (23.7 to 28.4 mg $kg^{-1}$) than without shade (20.7 to 25.8 mg $kg^{-1}$). However, total flavonoids [mg naringin equivalents $kg^{-1}$ DW] was more detected in the shoot part (17.4 to 26.8 mg $kg^{-1}$) than in the root part (6.7 to 7.0 mg $kg^{-1}$) of Panax ginseng. Methanol extracts from the Panax ginseng plants dose-dependently increased DPPH free radical scavenging activity as a antioxidant potential, and the activity was higher in shoot parts (31.1 to 36.5%) than in root parts (24.9 to 28.7%), and without shade than with shade. In conclusion, shade affects plant growth as well as physiological activity of Panax ginseng under plastic house condition.

• Korean Journal of Weed Science
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• v.8 no.1
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• pp.64-70
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• 1988

An experiment was carried out to know the effects of paclobutrazol on the lodging related traits and yield of a japonia (Seomjinbyeo) and an indica (Samgangbyeo) rice variety grown at 3 N levels (10, 20, 30 kg/10a) and 3 N split rates [basal + top dressing 15 days after transplanting (DAT) : top dressing 25 days before heading (DBH) was 100 : 0, 80 : 20, and 60 : 40]. Paclobutrazol (3kg/10a of 0.6% G) was applied 15 DBH and lodging related traits were observed 3, 13, 23, and 33 days after heading (DAH). The results obtained summarized as follows. 1. Culm length was increased as N level increased, but decreased by application of paclobutrazol. Culm length was not affected by N split rates in both varieties. 2. Lodging was not occurred at all plots, but lodging index of Seomingbyeo was much greater than that of Samgangbyeo. In both varieties loding index increased up to 23 DAH due to an increase in fresh weight and a decrease in breaking strength and levelled off thereafter due to a decrease in both fresh weight and breaking strength. 3. Culm length of Seomjinbyeo and fresh weight of shoot and culm length of Samgangbyeo were positively correlated with lodging index, but breaking strength was not correlated with lodging index in both varieties during the most of ripening stages. Direct effect of culm length contributing to lodging index was positive and much greater than that of fresh weight and breaking strength in Seomjinbyeo, but in Samganbyeo direct effect of culm length and fresh weight was positive, but that of breaking strength was negative with similar magnitudes. 4. Yield in brown rice of Seomjinbyeo was not affected by N level, but split application of N increased yield. In contrast, yield of Samgangbyeo was increased as N level increased, but yield was not affected by N split rates. Paclobutrazol did not affect yield of rice in Seomjinbyeo, but decreased yield by 5.2% in Samgangbyeo due to a decrease in the number of spikelets per panicle.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.3
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• pp.195-200
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• 1990

These experiments were conducted to determine the effect of high temperature and water managements on growth and yield of rice. Shoot dry weight and percent of fresh roots were decreased when rice plant was exposed to high temperature, and also high temperature treatment decreased yield components of rice through spikelet number at meiotic, filled grain ratio and percent of fertility at heading, and 1,000 grain weight at ripening stage. Nitragen content of the rice varities was decreased by high temperature treatment regardless different growth stage, although Si content increased. Grain yield of rice varieties significantly decreased due to high temperature when rice plants were treated at heading stage followed by ripening stage. meiotic, young panicle initiation, maximum tillering and tillering stage in order. Effect of the continual submerging on temperature increased by 1.1$^{\circ}C$ at daytime and by 3.7$^{\circ}C$ at nighttime, but flowing water irrigation which can maintain optimum temperature reduced plant height and increased dry weight and percent of fresh roots. Flowing water irrigation showed higher yield by 4-8% compared to continual submerging method through increment of yield components such as spikelet number, filled grain ratio and 1,000-grain weight.

• Journal of Bio-Environment Control
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• v.16 no.4
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• pp.352-357
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• 2007

We investigated effects of concentration and duration were investigated in order to promote efficiency of $CO_2$ enrichment in winter. The treatments were conducted with two levels of $CO_2$ concentration, namely 400 ppm, 700 ppm, two levels of duration, 3 h (9:00-12:00), 6 h (9:00-15:00), and control (nonenrichment $CO_2$). Fresh weight of leaves increased under longer exposure to $CO_2$ and higher $CO_2$ concentration. Fresh weight of stem and root increased under longer exposure to $CO_2$, but decreased under higher $CO_2$ concentration. Total dry weight increased under longer exposure to $CO_2$ and higher $CO_2$ concentration. Combination treatment of longer exposure to $CO_2$ and higher $CO_2$ concentration showed the largest decrease of Root : Shoot dry weight ratio. The $700ppm{\times}6h$ treatment showed higher fruit number and yield than control. The results suggested that the growth under longer exposure to 400 ppm $CO_2$ was better than that under higher $CO_2$ concentration.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.229-233
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• 2010

This experiment was carried out to find optimum ratio of $NO_3^-$ to $NH_4^+$ in nutrient solution for the growth of Phalaenopsis hybrid and find the effect of the ratio of $NO_3^-$ to $NH_4^+$ on the flower stem (inflorescence) quality. The ratio of $NO_3^-$ to $NH_4^+$ of nutrient solution used in this experiment was 100% : 0%, 90% : 10%, 80% : 20% and 70% : 30%. Phalaenopsis showed better growth when $NH_4^+$ was supplied concurrently with $NO_3^-$ as nitrogen source than supplied with only $NO_3^-$. Especially, increasing the ratio of $NH_4^+$ from 0% to 10% the fresh weight and dry weight of Phalaenopsis hybrid was highest. But, the growth of shoot and root was diminished when the proportion of $NH_4^+$ in nutrient solution was increased from 10% to 30%. Inflorescence length, the number of inflorescence and flower per plant all increased as $NH_4^+$ increased from 0% to 10% but, decreased from 10% to 30%. These results suggest that the optimal ratio of $NO_3^-$ and $NH_4^+$ in nutrient solution for the growth of Phalaenopsis including inflorescence was founded to be 90%:10%.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.297-300
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• 2017

Plant factory can control artificially the environments for crop cultivation, so they can produce high quality agricultural products all year round. This study was carried to select suitable kohlrabi cultivar for hydroponics in a closed-type plant factory system. We used three cultivars of red kohlrabi, 'Asac kohl', 'Kolibri', and 'Purple king' as plant materials. The artificial light source was LED light, light intensity and photoperiod were $249{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and 12/12 hours (day/night period), respectively. Hydroponic cultivation type was used circulating deep flow technique. At 43 days after transplanting, fresh weight of whole plant and tuber and leaf area were not significantly different among cultivars. Shoot dry weight and tuber dry weight were highest in 'Asac kohl' cultivar, and number of leaves was highest in 'Purple king' cultivar. Sugar content and yield were highest in 'Asac kohl' cultivar. Considering the growth and marketable yields, 'Asac kohl' was the optimal kohlrabi cultivar for hydroponic cultivation in a closed-type plant factory system.

• Journal of People, Plants, and Environment
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• v.23 no.4
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• pp.445-453
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• 2020

Background and objective: Calcium chloride (CaCl₂) and sodium chloride (NaCl) are commonly used as a deicing agent in South Korea and penetrate the soil on the roadside, causing damage to plants. This study was conducted to investigate the salinity reduction effect of Pennisetum alopecuroides and the chemical characteristics of soil leachate. Methods: The plants were treated with five different concentrations of CaCl₂ (0, 1, 2, 5, and 10g·L^-1) and were grouped into the Cont., C1, C2, C5, and C10 groups. CaCl₂ of 200 m·L^-1 was sprayed to each plant once every two weeks. The growth of P. alopecuroides (plant height, leaf length, leaf width and the number of leaves) was measured. The level of EC and pH, and exchangeable cations (K⁺, Ca²⁺, Na⁺, and Mg²⁺) in the leachate of soil was monitored. Results: The pH of soil leachate decreased as the CaCl₂ concentration increased, and the EC increased significantly. The content of K⁺ did not change significantly until the concentration of CaCl₂ reached 5 g·L^-1, but the content of Ca²⁺, Na⁺, and Mg²⁺ significantly increased. The plant height, leaf length, and leaf width of P. alopecuroides showed the highest value in CaCl₂ 1 g·L^-1 followed by CaCl₂ 2 g·L^-1 and the control group. Root fresh weight was the highest in CaCl₂ 2 g·L^-1. On the other hand, there was no change in the shoot fresh weight, dry weight and root dry weight, and P. alopecuroides growth inhibition at the concentration of 5 g·L^-1 or higher in the plant height and leaf length. Conclusion: P. alopecuroides is relatively highly salt-tolerant and can improve the salt damaged soil by lowering the content of the salt-based exchangeable K⁺ ions.