The Journal of the Convergence on Culture Technology (문화기술의 융합)

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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Establishment of propagation system for in vitro calla plants (Zantedeschia spp.) by treatment of taurine

타우린 처리를 통한 칼라 기내 식물체 대량증식체계 확립

  • 이상희 (건국대학교 생명공학과) ;
  • 김영진 (강원도농업기술원 원예연구과) ;
  • 양환래 (건국대학교 생명공학과) ;
  • 김종보 (건국대학교 생명공학과)
  • Received : 2018.08.04
  • Accepted : 2018.09.27
  • Published : 2018.11.30
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Abstract

Zantedeschia spp. calla is very popular as a cut flower. It is very important to establish a micro propagation system through plant tissue culture with the problem that colored calla with various colors are low in natural reproduction rate and vulnerable to high temperature. In this study, we conducted the experiment by adding taurine to improve the growth of calla plant. When 20 mg/L of taurine was added with plant growth effect, 54.0 % of the cases of multiple shoots and 17.2 times of fresh weight were the most effective. Taurine 20 mg/L treatment showed 16.0 % and 39.2 %, respectively, than the untreated control. Taurine may contribute to mass propagation of elite breeding lines as well as an improvement of farm income by positively influencing the overall growth of calla plants, thereby positively affecting the establishment of the micro propagation system of calla shoot tips.

천남성과에 속하는 Z antedeschia spp. 칼라는 절화로서 매우 인기가 높다. 다양한 색상을 지닌 유색칼라는 자연번식률이 낮고 고온에 취약하다는 문제로 식물 조직배양을 통한 대량 증식 체계를 확립하는 것이 매우 중요하다. 본 연구에서는 칼라 기내 식물체의 생육을 향상시키고자 타우린을 첨가하여 실험을 수행하였다. 식물 생장 효과로 타우린을 20 mg/L 첨가했을 때 다신초는 54.0 %의 발생을 보였고, 생체중은 17.2배가 증가하여 가장 높은 효율을 보였다. 식물 생장에 악영향을 주는 갈변화에서는 타우린 20 mg/L 처리구가 16.0 %로 무처리구의 39.2 %보다 약 2배 이상의 갈변이 억제된 것을 보였다. 타우린은 전반적인 칼라 식물 생장을 촉진시켜 칼라 경정의 대량 증식체계 확립에 긍정적인 영향을 줌으로써 우량품종의 효율적인 대량증식 및 농가 소득 향상에 도움이 될 것으로 판단된다.

Keywords

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그림 1. 타우린 첨가 배지에서 ‘각시’ 경정배양 8주 후 뿌리, 신초, 다신초 생장 비교 Figure 1. Comparison of growth for root, shoot, multiple shoots on various taurine medium after 8 weeks of culture in ‘Gagsi’ (A : MS medium; B : Taurine 2.5 mg/L; C : Taurine 5.0 mg/L; D : Taurine 10 mg/L; E : Taurine 15 mg/L; F : Taurine 20 mg/L)

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그림 2. 타우린 첨가 배지에서 ‘각시’ 경정배양 8주 후 생체중 증가 비교 Figure 2. Effect of different concentrations of taurine on fresh weight in scales of calla ‘Gagsi’ *Data were collected after 8 weeks of culture

표 1. ‘각시’에서 타우린 농도에 따른 다신초, 갈변율 형성 효과 Table 1. Effect of different concentrations of taurine on multiple shoots formation and browning in ‘Gagsi’

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