• Plant Resources
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• 제6권3호
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• pp.233-241
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• 2003

The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.

• Plant Biotechnology Reports
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• 제4권1호
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• pp.85-94
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• 2010

In vitro shoot regeneration of gladiolus in three different culture systems, viz., semi-solid agar (AS), membrane raft (MR), and duroplast foam liquid (DF) cultures was evaluated following the kinetics of shoot multiplication and hyperhydricity at optimized growth regulator combinations. Compared to the AS system, matrixsupported liquid cultures enhanced shoot multiplication. The peak of shoot multiplication rate was attained at 18 days of incubation in the MR and DF systems, whereas the maximum rate in the AS system was attained at 21 days. An early decline in acceleration trend was observed in liquid cultures than the AS culture. The hyperhydric status of the regenerated shoots in the different culture systems was assessed in terms of stomatal attributes and antioxidative status. Stomatal behavior appeared to be normal in the AS and MR systems. However, structural anomaly of stomata such as large, round shaped guard cells with damage in bordering regions of stomatal pores was pronounced in the DF system along with a relatively higher $K^+$ ion concentration than in the AS and MR systems. Antioxidative status of regenerated shoots was comparable in the AS and MR systems, while a higher incidence of oxidative damages of lipid membrane as evidenced from malondialdehyde and ascorbate content was observed in the DF system. Higher oxidative stress in the DF system was also apparent by elevated activities of superoxide dismutase, ascorbate peroxidase, and catalase. Among the three culture systems, liquid culture with MR resulted in maximum shoot multiplication with little or no symptoms of hyperhydricity. Shoots in the DF system were more prone to hyperhydricity than those in the AS and MR systems. The use of matrix support such as membrane raft as an interface between liquid medium and propagating tissue could be an effective means for rapid and efficient mass propagation with little or no symptoms of hyperhydricity.

• Journal of Plant Biotechnology
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• 제8권1호
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• pp.15-19
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• 2006

An efficient protocol for in vitro multiple shoot bud induction and plant regeneration from mature green cotyledon derived callus tissues of Acacia sinuata has been developed. Callus formation occurs at all the concentrations of thidiazuron (TDZ) in Murashige and Skoog's (MS) medium, but 0.6 ${\mu}M$ proved to be the best with maximum callus formation frequency. Supplementation of TDZ in combination with indole-acetic acid (IAA) in MS media accelerates shoot bud organogenesis in differentiating callus tissues with 60-70% conversion of shoot buds into shoot Most efficient shoot organogenesis was recorded when TDZ induced calli were subcultured at different concentrations of 6-benzyla-denine (BA). Optimum shoot bud induction and plant regeneration from callus was achieved when 0.6 ${\mu}M$ (TDZ) induced calli were subcultured at 3.0 ${\mu}M$ (BA) where $16.6{\pm}0.74$ shoots/unit callus on obtained. Rooting in in vitro differentiated shoots was achieved when transferred to medium containing different concentration of indole-3-butyric acid (IBA) in full & half strength MS medium. The well rooted plantlets were hardened and transferred to net house with 90% survival rate.

• 한국자원식물학회지
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• 제20권3호
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• pp.251-254
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• 2007

This study was conducted to investigate the optimal plant growth regulator level for the shoot formation of Lycium chinense Mill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants were cultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regenerated through in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shoot formation had developed from the leaf explants. The shoot formation rate of 'Jangmyeong' was highest followed by 'Myeongan', ;Cheondae', and 'Bullo'. The use of 0.2mg/L BA was critical for enhanced production of shoot formation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots were developed and successfully acclimatized to greenhouse.

• 식물조직배양학회지
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• 제24권1호
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• pp.43-48
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• 1997

고추냉이의 정단분열조직을 생장조절제 무처리, cytokinin류와 1.0 mg/L IAA를 혼용처리 및 cytokinin류를 단용 처리한 MS배지에 배양한 후 캘러스, shoot 및 뿌리발생률을 조사하였고 분화된 multiple shoot는 본엽 3~5매 정도에서 분할한 후 IAA와 IBA 단용 배지에 계대배양하여 뿌리형성에 적합한 생장조절제의 효과를 조사하였다. 생장조절제 무처리구의 경우 캘러스 유도없이 치상체에서 직접 shoot 및 뿌리발생이 이루어졌으나 배양 80일까지 shoot수는 2~3매로 생육이 저조하였다. Cytokinin류와 1.0 mg/L IAA 혼용처리의 경우 전 처리구가 100%의 캘러스가 유도되었으나 캘러스의 계속적인 증식은 없었고 대부분 캘러스로부터 다수의 뿌리가 형성된 후 배양 60일 후에 배양당시 부착된 엽원기가 shoot로 분화되는 경향이었는데 shoot수는 2~7개 정도였고 1개의 치상체당 multiple shoot수는 2~3개 정도였다. 1.0 mg/L zeatin과 1.0 mg/L IAA 혼용처리구의 경우 뿌리형성 후 shoot가 분화되어 본엽 4~5매의 완전한 식물체가 재분화되었다. Cytokinin류 단용 처리는 생장조절제의 종류와 무관하게 배양 5~10일 경부터 100%의 shoot가 분화된 후 증식되었고 배양 90일 후에는 다수의 multiple shoot로 증식되었는데 BA와 kinetin의 경우 1.0 mg/L에서 zeatin은 2.0 mg/L에서 multiple shoot분화율이 가장 높았다. 그러나 shoot로부터 뿌리분화는 극히 저조하여 완전한 식물체로의 재분화수는 IAA혼용처리에 비해 저조하였으나 shoot 분화에는 cytokinin류 단용 처리가 훨씬 효과적이었다. Multiple shoot를 본엽 3~4매에서 액아를 붙여 분할하여 뿌리분화율을 조사한 결과 IAA처리구보다 IBA처리구가 효과적이었는데 특히 0.01 mg/L IBA의 경우 계대배양 60일 후 93.3%의 뿌리 분화와 2~3개의 multiple shoot로 증식되어 shoot로부터 뿌리형성에 가장 좋은 결과였다.

• 식물조직배양학회지
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• 제25권1호
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• pp.63-68
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• 1998

정단 및 마디조직을 이용한 지황의 기내증식방안을 마련하기 위하여 일련의 시험을 실시하였다. 재생된 신초의 정단을 재배양하였을 경우 BA 5.0 mg/L에서 7.8개의 신초가 형성되었으며 투명화된 묘의 발생은 Gelrite 보다는 Bacto-agar 첨가구에서 감소하였다. 광도와 한천의 농도가 증가할수록 신초형성수 및 생장은 억제되었고 생체중 : 건물중의 비율도 감소하였다. 광도가 2000 lx일 때는 Bacto-agar 0.6-1.2%, 3,000 lx에서는 한천 0.4-0.6%에서 건전한 신초를 10개 이상 생산할 수 있었다. 활성탄소 0.1-0.3%첨가는 신초의 생장촉진과 뿌리형성에 촉진적으로 작용하였으며 투명화 발생률도 감소시켰으나 신초형성수는 현저히 감소시켰다. MS배지내 첨가되는 다량원소의 농도를 0.8-1.0배 하였을때 신초형성수 및 생장이 촉진되었다. 기관형성에 미치는 오옥신의 종류 및 농도별 효과를 조사한 결과 NAA나 IBA 보다는 IAA 0.3mg/L 와 BA 5.0mg/L를 혼합처리 하였을 때 증식률이 16배에 달하였다.

• 한국자원식물학회지
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• 제11권1호
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• pp.1-8
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• 1998

This study was conducted to establish mass propagation system from the axillary bud culture of chrysanthemum zawadskii H. which was used as material of medicinal plants. Shoot egeneration was better on MS medium with NAA and BA. The optimum concentraions of growth regulator for shoot regeneration differed depending on accessionsof C. Zawadskii. Shoot regeneration in Keungucheolcho was better on MS Medium with NAA 0.01mg/1 and BA 0.1mg/1 while Hyangrobonggucheocho was better with NAA 0.1mg/1and BA 0.3mg/1. Addition of NAA into medium was effective for induction of root from shoots regenerated. Shoot multiplcation was more effective when 10mg/1 spermine was added into medium than when other polyamines were treated ino medium . Randomly and specifically amplified polymorphic DAC banding patterns based on polymerase chain reaction (PCR) analysis were used to assess the genetic variation of plants regenerated from in vitro culture.

• Journal of Plant Biotechnology
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• 제50권
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• pp.176-182
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• 2023

사과 '후지'의 잎 절편체로부터 신초 기관형성을 통한 효율적인 식물체 재생 시스템을 확립하기 위하여 암처리 기간, 전처리 방법, 배양 용기당 치상 절편체 수, 배양 용기의 종류와 배지 위의 절편체 치상방향 등을 달리하여 실험을 실시하였다. 배양 초기 암조건에서의 배양은 신초 형성에 필수적이며, 4주 동안 배양한 후 명조건으로 옮겨 배양하는 것이 신초 재생에 가장 효과적이었다. 배양 전 전처리로는 sorbitol 40 g/L가 포함된 액체 재생배지에 2시간 동안 침지하였을 때 신초 형성율이 87.5%까지 증가하였으며, 신초 형성 시기가 빨라지는 것으로 나타났다. 배양 용기당 치상 절편체 수는 9개를 치상하는 것이 신초 재생에 효과적이었으며, 배양 용기는 100 ml 삼각플라스크를 사용하는 것이 petri dish를 사용하는 것에 비해 신초 형성율이 약 3배 정도 증가하였다. 잎절편체의 배지 위 치상방향은 배축면이 배지에 닿게 치상했을 때, 신초 형성율과 재생된 신초수가 다소 높게 나왔다. 암조건에서 4주간 배양한 후 명조건으로 옮겨총8주간 배양하여 재생된 신초는 1/4 MS에 0.2 mg/L의 IBA가 첨가된 배지에서 발근을 유도한 후 활착시켜 온실에서 재배하였을 때 정상적인 표현형을 보여주었다.

• 식물조직배양학회지
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• 제27권1호
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• pp.63-69
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• 2000

Buffalo gourd의 기내배양 체계를 확립하기 위하여, 기내 발아, 증식, 발근에 적합한 생장조절제와 배양환경 조건을 구명코자 본 실험을 수행하였다. Buffalo gourd의 종자는 암상태가 명상태 보다 더 높은 발아율을 얻을 수 있었다. BA 1.0 mg/L에 IAA 0.3-0.6 mg/L가 혼용처리된 MS 배지에서 신초증식 및 생육이 가장 양호했고 kinetin과 2iP는 신초증식에 큰 영향을 미치지 않았다. 증식을 위한 적정배양 온도는 22-$25^{\circ}C$ 였다. 기내발근은 오옥신 첨가로 쉽게 유도되었는데 특히 IBA 1.0-2.0mg/L 및 IAA 2.0mg/L 단용처리가 기내 발근에 가장 효과적이었으며, 지상부 생장도 양호하였다. 발근된 신초를 포장에 이식한 결과, 생존율이 90%이상에 달하였다. Common milkweed의 종자를 기내 파종하여 명상태에서 발아시킨 결과, 발아율이 71.2%로 비교적 높았다. 전반적으로 BA가 다른 사이토키닌 보다 기내생장에 더 효과적이었으나, 농도에 따라 효과는 달랐다. BA 3.0mg/L에 IAA 0.3 mg/L 혹은 0.6mg/L 혼용처리하는 것이 신초 생체중, 마디수의 증가에 효과적이었고 2iP는 신초신장에 효과적이였다. Com-mon milkweed의 기내생장은 온도에 아주 민감하였는데, 22 $^{\circ}C$에서 생장이 양호한 반면 $25^{\circ}C$ 이상에서는 생존율이 감소하였다. Common milkweed의 경우 기내 발근율이 매우 저조하였는데 배양전 신초를 IBA 10.0~100.0 mg/L용액에 30~120분간 처리 후 MS배지에 접종하였을 경우 발근율이 4~5%로 매우 낮았다.

• Journal of Plant Biotechnology
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• 제1권2호
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• pp.97-100
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• 1999

In order to establish in vitro propagation method of sundew, Drosera rotundifolia L., the effects of MS medium concentration, cytokinin type and concentration, pH, and auxin type and concentration on shoot proliferation and root formation were investigated using shoots at 3 month after seed germination. The highest shoot production was obtained with the half strength of MS ($\frac{1}{2}$ MS) medium than with any other strength of MS medium tested. Addition of kinetin or BA in $\frac{1}{2}$ MS medium was strongly suppressed shoot proliferation. The suppression of shoot proliferation was more effective in BA-supplemented $\frac{1}{2}$ MS medium than kinetin-supplemented. The optimum pH of the media for shoot proliferation was pH 5.7-6.7. Shoots were subcultured in $\frac{1}{2}$ MS medium supplemented with 0.5mg/L 2,4-D for rooting every 8 weeks. All subcultured shoots produced extensive root systems after 5 to 6 week culture. Plantlets after root development were planted in plastic pots filled with moss. The survival rate of plantlets was almost 100%. On subculturing every 8 weeks, hundreds of the plants were propagated from a single plant within a year.