• BMB Reports
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• v.40 no.4
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• pp.554-561
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• 2007

Shen and colleagues (Lin et al., 2004) have recently shown that overexpression of the Drosophila DNA methyltransferase 2 isoform C, dDnmt2c, extended life span of fruit flies, probably due to increased expression of small heat shock proteins such as Hsp22 or Hsp26. Here, I demonstrate with immunoprecipitations that overexpressed dDnmt2c interacts with endogenous Hsp70 protein in vivo in S2 cells. However, its C-terminal half, dDnmt2c(178-345) forms approximately 10-fold more Hsp70-containing protein complexe than wild-type dDnmt2c. Overexpressed dDnmt2c(178-345) but not the full length dDnmt2c is able to increase endogenous mRNA levels of the small heat shock proteins, Hsp26 and Hsp22. I provide evidence that dDnmt2c(178-345) increases Hsp26 promoter activity via two heat shock elements, HSE6 and HSE7. Simultaneously overexpressed Hsp40 or a dominant negative form of heat shock factor abrogates the dDnmt2c(178-345)-dependent increase in Hsp26 transcription. The data support a model in which the activation of heat shock factor normally found as an inactive monomer bound to chaperones is linked to the overexpressed C-terminus of dDnmt2c. Despite the differences observed in flies and S2 cells, these findings provide a possible explanation for the extended lifespan in dDnmt2c-overexpressing flies with increased levels of small heat shock proteins.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.2
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• pp.122-129
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• 2004

There is an increasing need for large flat panel display devices. PDP (Plasma Display Panel) is one of the most promising candidates for this need. Thermal shock failure of PDP glass during manufacturing process is a critical issue in PDP industry since it is closely related to the product yield and the production speed. In this study, thermal shock resistance of PDP glass is measured by water quenching test and an analysis scheme is described for estimating transient temperature and stress distributions during thermal shock. Based on the experimental data and the analysis results, a simple procedure for predicting the thermal shock failure of PDP glass is proposed. The fast cooling process for heated glass plates can accelerate the speed of PDP production, but often leads to thermal shock failure of the glass plates. Therefore, a design guideline for preventing the failure is presented from a viewpoint of high speed PDP manufacturing process. This design guideline can be used for PDP process design and thermal -shock failure prevention.

• 한국전산유체공학회:학술대회논문집
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• 2000.05a
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• pp.39-44
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• 2000

Two-dimensional Navier-Stokes codes are used to simulate the shock tunnel in Konkuk university. In order to design experiments in impulse facilities properly and to interpret data from such facilities, it is necessary to understand how the flow approaches steady state. This is done by determining the transient flow field and flow establishment time around a given model. This will be accomplished by developing appropriate CFD codes which solve the Navier-Stokes equations, and simulating the starting process and resulting unsteady viscous flow phenomena. The starting process in a shock tunnel consists of multiple shock interactions and contact discontinuities, which are difficult to solve with the classical shock capturing schemes. A recently developed high resolution scheme is adapted for resolving the unsteady phenomena of those multiple shock interactions and contact surfaces during the starting process. The bifurcation phenomenon due to the interactions of the reflected shock from the end of the shock tube with the boundary layer generated by the incident shock becomes of particular interest. By comparing with the experiment results, the accuracy of the numerical analysis is validated and it is demonstrated that the properties which can hardly be obtained through the experiment can be estimated.

• Journal of Oriental Neuropsychiatry
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• v.11 no.1
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• pp.37-46
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• 2000

We investigated the effects of lemon pure essential oils on the heat shock-induced apoptosis in human astrocyte cell line CCF-STTG1. In previous studies, hear shock has been reported to induce the apoptosis or programmed cell death through the activation of caspase-3. Treatment of CCF-STTG1 cells with heat shock markedly induced apoptotic cell death as determined by flow cytometry. Interestingly, pretreatment of CCF-STTG1 cells with lemon pure essential oils inhibited the heat shock-induced apoptosis. Lemon also inhibited the heat shock-induced apoptosis in primary cultured rat astrocytes. To determine whether lemon inhibits the heat shock-induced activation of these apoptotic proteases, activation of CPP32 was assessed by Western blotting. Consistent with flow cytometry, DNA fragmentation and giemsa staining, heat shock-induced activation of CPP32 was blocked by lemon pure essential oil. PARP, cysteine protease substrates were fragmented as a consequence of apoptosis by heat shock. Lemon oil inhibited the PARP fragmentation. This essential oil also inhibited the heat shock-induced activation of caspase-3. These results suggest that lemon pure essential oils may modulate the apoptosis through the activation of the ICE-like caspases.

• Journal of the Korean Society of Visualization
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• v.10 no.2
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• pp.32-38
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• 2012

In recent years a unique drug delivery system named as the transdermal drug delivery system has been developed which can deliver drug particles to the human skin without using any external needle. The solid drug particles are accelerated by means of high speed gas flow through a shock tube imparting enough momentum so that particles can penetrate through the outer layer of the skin. Different systems have been tried and tested in order to make it more convenient for clinical use. One of them is the contoured shock tube system (CST). The contoured shock tube consists of a classical shock tube connected with a correctly expanded supersonic nozzle. A set of bursting membrane are placed upstream of the nozzle section which retains the drug particle as well as initiates the gas flow (act as a diaphragm in a shock tube). The key feature of the CST system is it can deliver particles with a controllable velocity and spatial distribution. The flow dynamics of the contoured shock tube is analyzed numerically using computational fluid dynamics (CFD). To validate the numerical approach pressure histories in different sections on the CST are compared with the experimental results. The key features of the flow field have been studied and analyzed in details. To investigate the performance of the CST system flow behavior through the shock tube under different operating conditions are also observed.

• Proceedings of the KSME Conference
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• 2004.04a
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• pp.1643-1648
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• 2004

When a shock wave propagates into a Helmholtz resonator, very complicated wave phenomena are formed both inside and outside the resonator tube. Shock wave reflection, shock focusing phenomena and shock-vortex interactions cause strong pressure fluctuations inside the resonator, consequently leading to powerful sound emission. In the present study, the wave phenomena inside and outside the Helmholtz resonator are, in detail, investigated with a help of CFD. The Mach number of the incident shock wave is varied below 2.0 and several types of resonators are tested to investigate the influence of resonator geometry on the wave phenomena. A TVD scheme is employed to solve the axisymmetric, compressible, Euler equations. The results obtained show that the configuration of the Helmholtz resonator significantly affects the peak pressure of shock wave focusing, its location, the amplitude of the discharged wave and resonance frequency.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.22 no.9
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• pp.1229-1237
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• 1998

Experiments of shock-wave/turbulent boundary layer interaction were conducted by using a supersonic wind tunnel. Nominal Mach number was varied in the range of 1.6 to 3.0 by means of different nozzles. The objective of the present study is to investigate the effects of boundary layer suction on normal shock-wave oscillations caused by shock wave/boundary layer interaction in a straight duct. Two-dimensional slits were installed on the top and bottom walls of the duct to bleed turbulent boundary layer flows. The bleed flows were measured by an orifice. The ratio of the bleed mass flow to main mass flow was controlled below the range of 11 per cent. Time-mean and fluctuating wall pressures were measured, and Schlieren optical observations were made to investigate time-mean flow field. Time variations in the shock wave displacement were obtained by a high-speed camera system. The results show that boundary layer suction by slits considerably reduce shock-wave oscillations. For the design Mach number of 2.3, the maximum amplitude of the oscillating shock-wave reduces by about 75% compared with the case of no slit for boundary layer suction.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.20 no.6
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• pp.63-68
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• 2006

Recent statistical data regarding electric shock accidents have been analyzed to examine the electric shock accidents occurred at the voltage of 22,900[V], In order to demonstrate the mechanism of the 22,900[V] electric shock accident, a number of experiments to simulate electric shock accidents have been performed based on the analysis results. In the experiment, the current flowing through a human body model was measured to quantitatively analyze the hazards of the simulated electric shock accidents in various situations. As a result of the experiment, it was shown that once an electric shock accident occurred the accident proved fatal to the human body, regardless of electric shock situation.

• Journal of muscle and joint health
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• v.29 no.1
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• pp.60-68
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• 2022

Purpose: This study aimed to investigate the changes in reality shock among new nurses and their influencing factors to identify turnover reduction methods. Methods: A total of 146 new nurses from 6 general hospitals participated in the survey. The survey data were collected from August 5 to November 10, 2015. Results: The mean scores for factors related to turn over were 2.75 for nurse practice environment, 2.84 (4point scale) for reality shock, and 3.08 (5point scale) for turnover intention. A significant difference in the impact of reality shock was observed depending on whether the nurses selected their work unit (F=6.24, p=.003) and whether they could take the possibility of holiday on the desired day at will (t=-2.57, p=.013). Changes in reality shock correlated with turnover intention, with the 'increased reality shock' group demonstrating an odds ratio of 2.37 (CI: 1.41~3.98) for turnover intention. Conclusion: The current findings indicate the need for lowering reality shock and turnover intention by considering nurses' work-related characteristics and improving their practice environments. To further study the changes in reality shock new nurses experienced, additional research is warranted while homogenizing the participants in terms of preceptorship duration and timing of independence.

• Convergence Security Journal
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• v.12 no.5
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• pp.51-59
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• 2012

Purpose of this study analyzes deeply and examine closely effect that incident shock of private guards gets in job stress. Used participation observation and In-depth analytical method to 10 common people guards inhabiting in capital region in 2012 to achieve purpose of this study. Collected interview data encoded using computer and analyzed by incident shock, job effect, job stress, organizational life. Conclusion proved in this study is as following. First, private guards appeared that incident shock such as suicide, terror threat, irrationality of superior that is produced in business spot gives great psychological fear. Second, private guards appeared suffer important shock are influenced in fear about business from shock that suffered in the past, and sociophobia appeared by thing which exert important effect changing by other business territory. Third, most private guards appeared that oneself experienced job stress from experiencing incident shock in the past. That is, incident shock exerts direct influence on customer confrontation in the past. Fourth, private guards appeared by thing which individual's organization life reduces when do not overcome incident shock that occur in the past. That is, incident shock decreases trust development and teamwork between superior and subordinate in organizational culture of private security.