• KSBB Journal
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• v.30 no.6
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• pp.307-312
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• 2015

Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and $k_La$. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.

• International Journal of Fluid Machinery and Systems
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• v.9 no.3
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• pp.229-236
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• 2016

Exergetic analysis was introduced in optimization of a rotating equilateral triangular internal cooling channel with staggered square ribs to maximize the net exergy gain. The objective function was defined as the net exergy gain considering the exergy gain by heat transfer and exergy losses by friction and heat transfer process. The flow field and heat transfer in the channel were analysed using three-dimensional Reynolds-averaged Navier-Stokes equations under the uniform temperature condition. Shear stress transport turbulence model has been selected as a turbulence closure through the turbulence model test. Computational results for the area-averaged Nusselt number were validated compared to the experimental data. Three design variables, i.e., the angle of rib, the rib pitch-to-hydraulic diameter ratio and the rib width-to-hydraulic diameter ratio, were selected for the optimization. The optimization was performed at Reynolds number, 20,000. Twenty-two design points were selected by Latin hypercube sampling, and the values of the objective function were evaluated by the RANS analysis at these points. Through optimization, the objective function value was improved by 22.6% compared to that of the reference geometry. Effects of the Reynolds number, rotation number, and buoyancy parameter on the heat transfer performance of the optimum design were also discussed.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.28 no.7
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• pp.879-886
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• 2004

A numerical optimization has been carried out to determine the shape of the three-dimensional channel with oblique ribs attached on both walls to enhance turbulent heat transfer. The response surface based optimization is used as an optimization technique with Reynolds-averaged Navier-Stokes analysis of fluid flow and heat transfer. Shear stress transport (SST) turbulence model is used as a turbulence closure. Numerical results fur heat transfer rate show good agreements with experimental data. four dimensionless variables such as, rib pitch-to-rib height ratio, rib height-to-channel height ratio, streamwise rib distance on opposite wall to rib pitch ratio, and the attack angle of the rib are chosen as design variables. The objective function is defined as a linear combination of heat-transfer and friction-loss related coefficients with a weighting factor. D-optimal method is used to determine the training points as a means of design of experiment. Sensitivity of the objective parameters to each design variable has been analyzed. And, optimal values of the design variables have been obtained in a range of the weighting factor.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2448-2453
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• 2008

The present study numerically investigates the flow and heat transfer characteristics of rib-induced secondary flow in a cooling channel with staggered V-shaped ribs, extruded on both walls. The rib-height-to-hydraulic diameter ration (h/$D_h$) is 0.17; the rib pitch-to-height ratio (p/h) equals 2.8; the Reynolds number is 50,000. Shear stress transport (SST) turbulence model is used as a turbulence closure. The present results are compared with those for a continuous V-shaped rib. Computational results show that, for average heat transfer rate the staggered V-shaped rib gives about 2.5 times higher values than the continuous V-shaped rib, while, for the streamwise pressure drop the former gives about 5 times higher values than the latter. Consequently, for the thermal performances, based on the equal pumping power condition, the staggered one gives about 2 times higher values than the continuous one. Also, for the staggered V-shaped rib, complex secondary flow patterns are generated in the duct due to the snaking flow in the streamwise direction, and more uniform heat transfer distributions are obtained.

• Structural Engineering and Mechanics
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• v.16 no.4
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• pp.493-517
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• 2003

Attempts at improving beam-column joint performance has resulted in non-conventional ways of reinforcement such as the use of the crossed inclined bars in the joint area. Despite the wide accumulation of test data, the influence of the crossed inclined bars on the shear strength of the cyclically loaded exterior beam-column joints has not yet been quantified and incorporated into code recommendations. In this study, the investigation of joints has been pursued on two different fronts. In the first approach, the parameters that influence the behaviour of the cyclically loaded beam-column joints are investigated. Several parametric studies are carried out to explore the shear resisting mechanisms of cyclically loaded beam-column joints using an experimental database consisting of a large number of joint tests. In the second approach, the mechanical behaviour of joints is investigated and the equations for the principal tensile strain and the average shear stress are derived from joint mechanics. It is apparent that the predictions of these two approaches agree well with each other. A design equation that predicts the shear strength of the cyclically loaded exterior beam-column joints is proposed. The design equation proposed has three major differences from the previously suggested design equations. First, the influence of the bond conditions on the joint shear strength is considered. Second, the equation takes the influence of the shear transfer mechanisms of the crossed inclined bars into account and, third, the equation is applicable on joints with high concrete cylinder strength. The proposed equation is compared with the predictions of the other design equations. It is apparent that the proposed design equation predicts the joint shear strength accurately and is an improvement on the existing code recommendations.

• Journal of the Korean Society for Precision Engineering
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• v.28 no.6
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• pp.745-750
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• 2011

In a hope to better understand the flow and convective heat transfer characteristics inside a ball bearing, air flow between the rolling elements and raceways at high speed bearing rotation is numerically investigated using a simplified inner geometry of bearing and a CFD technique. Flow simulation results reveal the pressure distribution of airflow and the shear stress distribution on the ball surface, of which nonuniformity becomes significant with the increasing rotational speed. Also, the local point of maximum shear stress coincides with the stagnation flow area on the surface of rolling elements. A complex pattern of three-dimensional vortex structures is found in the air flow due to the relative motion of bearing elements and three different types of vortex pairs exist around the rotating and orbiting rolling elements.

• Geomechanics and Engineering
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• v.31 no.1
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• pp.53-69
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• 2022

In densely built areas, the development of underground transportation systems often involves twin excavations, which are sometimes unavoidably constructed adjacent to existing piled foundations. Because soil stiffness degrades with induced stress release and shear strain during excavation, it is vital to investigate the piled raft responses to subsequent excavation after the first tunnel in a twin-excavation system. The effects of deep excavations on existing piled foundations have been extensively investigated, but the influence of twin excavations on a piled raft is seldom reported in the literature. In this study, three-dimensional numerical analyses were carried out to investigate the influence of sand density on an existing piled raft (with a working load on top of the raft) due to twin excavations. A wide range of relative density (D_r) from loosest (30%), loose to medium (50% and 70%), and densest (90%) were selected to investigate the effects on settlement and load transfer mechanism of the piled raft during twin excavations. An advanced hypoplastic sand model (which can capture small-strain stiffness and stress-state dependent dilatancy of sand) was adopted. The model parameters are calibrated against centrifuge test results in sand reported in the literature. From the computed results, it is found that twin excavations in loose sand (D_r=30%) caused the most significant settlement. This is because of the higher stiffness of denser sand (D_r=90%) than that of loose sand. In contrast, a much larger tilting (maximum magnitude=0.18%) was computed in dense sand than in loose sand after the completion of the first excavation. As far as the load transfer mechanism along the piles is concerned, an upward load transfer to mobilize shaft resistance is observed in loose sand. On the contrary, a downward load transfer is observed in dense sand.

• Journal of the Korean Society for Precision Engineering
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• v.12 no.3
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• pp.110-118
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• 1995

The filling phase analysis of the injection molding process for thermoplastics was applied to predict pressure, themperature and shear stress in the test mold, and the results were compared with the experiment using 30% glass fiber added ABS resin. The finite difference method was used in the analysis considering the effects of heat transfer between molten polymer and mold wall, and also frictional heating by shear flow. The analysis results were considered as a method to improve the quality and the productivity of injection molding process. Using the analysis results, the molding factors such as mold-ability of polymers, performance of injection molding machine, positioning of gate and dimendsioning of runner in the injection molding process can be estimated at the design stage of mold for good quality and productivity.

• Journal of Convergence for Information Technology
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• v.9 no.8
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• pp.155-163
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• 2019

Laminar mixed convection of a nanofluid consists of water and $Al_2O_3$ in a horizontal circular tube has been studied numerically. Two-phase mixture model has been used to investigate hydrodynamic and thermal behaviors of the nanofluid with variables physical properties. Three dimensional Navier-Stokes, energy and volume fraction equations have been discretized using the finite volume method. The Brownian motions of nanoparticles have been considered to determine the thermal conductivity and dynamic viscosity of $Al_2O_3$-Water nanofluid, which depend on temperature. The calculated results show good agreement with the previous numerical data. Results show that in a given Reynolds number (Re), increasing solid nanoparticles volume fraction and Richardson number (Ri) increases the convective heat transfer coefficient and wall shear stress.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.4
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• pp.576-582
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• 2015

The efficiencies of thermal power system using fossil fuel depend on heat exchangers which extract energy from the exhaust gas before it is expelled to the atmosphere. To increase heat transfer efficiency it is very important to maintain the surface of heat exchanger as clean condition. The accepted skill of cleaning of fouled surface of heat exchanger is soot blowing. A high pressure jet of air is forced through the flat surface of plate to remove the deposit of fouling. There is, however, little knowledge of the fundamental principles of how the jet behave on the surface and how the jet actually removes the deposit. Therefore, the study focuses on the measuring of cleaning area and cleaning dwell time after accumulating the simulated deposit on the flat surface. The deposit test rig was built for the study and simulated deposit material is used after measuring the physical property of the each material by shearing stress test. Much data was obtained for the analysis by the parameters change such as the different jet speed, different inner pressure and variable distance of the jet from the test rig surface. The experimental data was compared with the theoretical equation and most of the data matches well except some extreme cases.