• Title/Summary/Keyword: shark

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Hot Water Extraction of Crude Chondroitin Sulfate from Shark Cartilage Treated with Ozone Water (오존수로 세척한 상어연골로부터 Crude Chondroitin Sulfate의 열수추출)

  • Jung, Yoo-Kyung;Shin, Kyung-Ok;Shin, Jong-Wook;No, Hong-Kyoon;Kim, Soon-Dong
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.3
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    • pp.149-154
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    • 2007
  • This study was conducted to investigate the effect of ozone treatment on removal of brown color and ammonia-like odor produced from shark cartilage (SC) and to establish the optimal extraction conditions of chondroitin sulfate (CS) by NaOH and hot water. Treatment of SC with 0.3 ppm ozone water resulted in higher color $L^*$ value but lower $a^*$ and $b^*$ values compared with those of non-treated SC, without affecting its proximate compositions (moisture, protein, lipid, carbohydrate, and ash). Ozone treatment also increased the overall acceptability of SC by reducing ammonia-like odor. The optimum concentration and time for extraction of CS from SC using NaOH at $100^{\circ}C$ were 1 N and 120 min, respectively, with 10.02% of extraction yield. The total yield (10.01%) of CS obtained by 7 times repeated extraction with hot water at $100^{\circ}C$ was comparable to that of the 1 N NaOH extraction for 120 min at $100^{\circ}C$.

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Catch characteristics of the Korean tuna longline fishery in the Atlantic ocean (대서양 해역 한국 다랑어 연승어업의 어획특성)

  • Pakr, Hee-Won;Yoon, Sang-Chul;Kim, Zang-Geun;Lee, Sung-Il;Jeong, Yeon-Kyu;Lee, Dong-Woo
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.50 no.4
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    • pp.556-566
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    • 2014
  • Korean distant water tuna longline fishery commenced in 1966 in the Atlantic Ocean. Since then, it has become one of the most important fisheries of Korea. By early of 1970s, total amount of tuna caught in the Atlantic Ocean was the highest among other Korean distant water tuna fisheries, but has become minor since 1990s. The annual catch of tuna and tuna-like species by Korean tuna longline fishery in the Atlantic Ocean was about 1,900 mt in 2013. Bigeye tuna was the predominant species in species composition followed by yellowfin tuna, Albacore tuna, Blue shark and Swordfish. Korean distant water tuna longline fishery have mainly operated in the tropical area of the Atlantic Ocean ($20^{\circ}N{\sim}20^{\circ}S$, $20^{\circ}E{\sim}60^{\circ}W$), fishing ground was almost similar as in the previous years. The length frequency of major species (Bigeye tuna, Yellowfin tuna, Albacore tuna, Blue shark and Swordfish) were estimated. As the result of length (size) frequency data on main species caught by lonline fishery in the Atlantic Ocean, main length intervals of bigeye tuna caught in 2011, 2012 and 2013 were 120~125 cm, 160~165 cm and 130~135 cm, respectively. For yellowfin tuna, those were 125~130 cm, 150~155 cm, 145~150 cm and for albacore, 109 cm, 102 cm, 109 cm and 106 cm respectively. For swordfish caught in 2011, 2012 and 2013, main length intervals were 130~135 cm, 125~135 cm and 125~130 cm, respectively, and for blue shark, 195~200 cm in 2011 and 185~190 cm in 2012, 2013.

Lipoid Pneumonia After Aspiration of Squalene$^{(R)}$ in Rabbit (토끼에서 Squalene$^{(R)}$ 흡인에 의한 지방성 폐렴)

  • Lee, Seong-Eun;Baik, Jae-Joong;Chung, Yeon-Tae;Chang, Hee-Jin
    • Tuberculosis and Respiratory Diseases
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    • v.46 no.1
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    • pp.75-81
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    • 1999
  • Background : Recently, there are many patients with lipoid pneumonia by ingestion of shark liver oil in Korea, but only a few animal experimentations have been carried out. The purpose of this study is to evaluate sequential change of the lung after aspiration of shark liver oil and to provide the radiologic-pathologic correlation. Methods: A single intratracheal administration of shark liver oil was given to 14 white rabbits. They were then sacrificed sequentially from 1 week to 6 weeks after injection. We investigated the HRCT and pathologic findings Results: One was sudden death immediately after injection. Six of the 13 rabbits showed pneumonic infiltrations on the HRCT. There were air space consolidation with air-bronchogram on the HRCT of the first week. They were associated with the volume loss in the 4th week, and the traction bronchiectasis in the 6th week. The important pathologic findings were peribronchial alveolar inflammation with septal widening and cuboidal metaplasia of the alveolar wall. The number of macrophages in an alveoli was peaked in the second week and then gradually decreased. On the 6th week, we could find the proliferation of fibroblasts. Conclusion: We can prove the development of lipoid pneumonia after aspiration of squalene by animal experimentation, and the understanding of HRCT and pathologic findings may be helpful in proper evaluation of pneumonia due to aspiration of fish-extracted lipid.

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A Study on Mercury Exposure Levels among Schoolchildren and Related Factors in High Mercury Exposure Areas in Korea (수은 고노출 지역 초등학생의 수은노출수준과 노출요인 연구)

  • Kim, Dae Seon;Kwon, Young Min;Chung, Hee-Ung;Nam, Sang Hoon;Yu, Seung Do
    • Journal of Environmental Health Sciences
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    • v.41 no.4
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    • pp.231-240
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    • 2015
  • Objectives: Blood mercury levels among adults living in certain areas of the Gyeongsang Provinces have been shown to be very high (Kunwee County $29.6{\mu}g/L$, Yeongcheon-city $26.7{\mu}g/L$). The purpose of this project was to determine mercury exposure levels in schoolchildren and factors related with their mercury levels in high mercury exposure areas identifyed by the 2007 Korea National Environmental Health Survey. Methods: From June to September 2010, 1,097 students from grades 3 to 6 at 19 elementary schools participated in this study, including 294 students from 10 elementary schools in Kunwee County, 529 students from Yeongcheon City, 122 students from two elementary schools in Pohang City, North Gyeongsang Province, and 152 students from two elementary schools in Ulsan Metropolitan City. Biological samples from schoolchildren, including whole blood, urine and hair, were collected to measure total mercury at the time of a health check up. Information about children was collected by questionnaire. Total mercury concentrations in blood were measured using the Direct Mercury Analyzer 80 with the gold-amalgam collection method. Results: The mean mercury levels were $2.70{\mu}g/L$ in 1,091 blood samples, $2.25{\mu}g/g-creat.$ in 820 urine samples and $1.03{\mu}g/g$ in 1,064 hair samples. Blood mercury levels in the schoolchildren was slightly higher than the result of $2.4{\mu}g/L$ from a 2006 survey of elementary school children on exposure and health effects of mercury by the National Institute of Environmental Research. However, 0.3% and 4.5% of participants exceeded the reference level of blood mercury by CHBMII ($15{\mu}g/L$) and the US EPA ($5.8{\mu}g/L$), respectively. The reference level of urine by CHBMII ($20{\mu}g/L$) was exceeded by 0.4% of participants. As factors, residence period in the study areas, residence type, father's education level and income all showed significant associations with mercury level in the biological samples. The number of dental amalgam sides showed an association with urine mercury. Fish intake preference and fish intake frequency were important factors in mercury levels. In particular, intake of shark meat and recent intake of shark meat were associated with higher mercury levels. In this regard, participation in the performance of an ancestral rite showed a relation with higher mercury levels. Conclusion: The intake of shark meat was very important factor to high mercury exposure level. It is recommended to monitor and manage students with high mercury exposures who exceeded CHBM II and EPA guidelines, and include blood mercury testing in the Children's Health check up for this province.

Packet Analysis with Snort & Suricata (Snort와 Suricata를 활용한 내부망 공격 탐지 및 패킷 분석)

  • Kim, Sang-yoo;No, Woo-Suk;Lee, Ho-Jae
    • Proceedings of the Korea Information Processing Society Conference
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    • 2017.11a
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    • pp.259-262
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    • 2017
  • 오픈소스 기반의 패킷 탐지 도구인 WireShark를 활용하여, 패킷을 직접 캡쳐, 분석해보고 칼리 리눅스를 활용한 시나리오 기반의 모의해킹을 시도, Snort와 Suricata를 이용하여 공격적인 성향의 패킷을 탐지하여 로그를 데이터베이스화 시킨 후 사용자 편의에 맞도록 가시화(可視化)한다. 사용자는 가시화 된 로그를 통해 공격을 좀 더 빠르게 인지하여 대응하는 것이 가능하다.

Estimation of the Ratio of Fin Weight to Body Weight of Sharks for the Korean Tuna Longline Fishery in the Eastern Pacific Ocean (동부태평양 한국의 다랑어 연승어업에서 상어류 체중대비 지느러미 중량 비율 추정)

  • An, Doo-Hae;Kwon, You-Jung;Moon, Dae-Yeon;Hwang, Seon-Jae;Kim, Soon-Song
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.2
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    • pp.157-164
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    • 2009
  • The National Fisheries Research and Development Institute (NFRDI) conducted a circle hook experiment to compare the catch rates of target and bycatch species between J hook and circle hooks in the tuna longline fishery of the Eastern Pacific Ocean, which is located between $9^{\circ}13'S-1^{\circ}36'N$ and $126^{\circ}00'-138^{\circ}21'W$. Sampling was conducted from September 20 to October 23, in 2006. In this survey, we collected shark data to obtain the ratio of fin weight to body weight of sharks. Overall, 413 sharks belonging to 9 species were caught on 62,464 hooks. The total weight of the sharks caught was 11,093 kg. The ratio of wet fin weight to body round weight of sharks by species ranged from 3.7% to 7.7%, while the mean was 5.4%. The total ratio of wet fin weight to the dry fin weight of sharks ranged 0.38 to 0.70 and the mean value was 0.53. The ratio of dry fin weight to the body round weight of the sharks was estimated to be 2.86%.

Design of Compact Data Integration and Convergence Device Using Esp8266 Module (Esp8266모듈을 이용한 소형 데이터 통합 및 융합장치 설계)

  • Lee, Dong-Seok;Lim, Joong-Soo
    • Journal of the Korea Convergence Society
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    • v.8 no.2
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    • pp.15-20
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    • 2017
  • In this paper, Esp8266, Node.js, and TCP / IP socket communication are used to design a compact data integration device. This device is designed to configure server and client using Esp8266 module that supports Wifi connection and to support bidirectional data transmission using TCP / IP socket communication. The server is configured using the Node.js operating system, and the database is integrated using Mysql. The network is designed to have a separate IP address by assigning a private IP address to the router, such as a home network. This device can transmit data bidirectionally, store individual client data on the server side, and can check the flow of data transmitted bidirectionally through wire-shark, so that it can be used as a compact real-time data integration and convergence device.

Cloning of a matrix metalloproteinase cDNA from Scylliorhinus torazame (두툽상어 matrix metalloproteinase 유전자 cDNA의 클로닝)

  • Kim, Jon Won;Cho, Won Jin;Chun, Kwang Ho;Kim, Kyu-Won;Kim, Yung-Jin;Lee, Sang-Jun;Shin, Hae-Ja;Lim, Woon Ki
    • Journal of Life Science
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    • v.8 no.3
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    • pp.235-240
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    • 1998
  • Matrix metalloproteinases(MMPs) are a group of zinc enzymes responsible for degradation of the matrix components such as collagen and proteoglycans in normal embryogenesis and remodeling and in many disease processes such as arthritis, cancer, periodontitis, and osteprocess. Genetically distince MMPs have been characterized and their genes have been cloned thus far from a variaty of species but not from fishes. In this stydy, a mmp cDNA was cloned by using RT-PCR(reverse transcriptase dependent polymerase chain reaction) from Scylliorhinus toraxzame(shark), agroup of cartilaginous fish, abundant in the coast of Pusan, Korea. It has 74% base homologue with membrane type matrix matalloproteinase-3 genes(mt3-mmps) from human, rat and chick, and also shows more than 90% residue homologue with them. In addition, it has cysteine switch domain, zinc binding domain(HExGH motif), propeptide cleavage site, and RRKR motif, which are present in MMPs. This result indicates that cDNA fragment cloned here may be mt3-mmp or its analogous gejne cDNA fragment of Scylliorhinus torzame.

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Active Shark Antenna for the Vehicle AM/FM/TDMB/GPS Receiver (자동차용 AM/FM/TDMB/GPS 통합 능동형 샤크 안테나)

  • Kim, Joo-Man;Son, Tae-Ho
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.6
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    • pp.698-705
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    • 2010
  • A vehicle antenna for AM, FM, TDMB, GPS systems was designed and implemented. Omnidirectional AM antenna was designed by ferrite turn style antenna. For the FM and TDMB antenna, folded monopole antenna which helical is folded was applied. GPS antenna for the bandwidth achievement was designed by trapezoidal microstrip patch that has air substrate. Receiving signal strengths by the measurement were presented for the AM, FM and TDMB antenna. AM signal strength was -65.7 dBm, this strength is almost as same conventional pole antenna as -63.4 dBm. It can be replaced conventional pole or glass antenna by the studied antenna. Signal strengths for FM and TDMB were -55.66 and -43.50 dBm at center frequency, they are 5~10 dB higher than conventional antenna. Measurements of bandwidth and gain for the GPS antenna showed 135 MHz under VSWR 2 : 1 and 4.31 dBi, gains over GPS band were 3~5 dB higher than ceramic patch antenna.

Molecular Phylogenetic Analyses of Scyliorhinus torazame (Carcharhiniformes) Inferred from Cu,Zn Superoxide Dismutase (두툽상어(Scyliorhinus torazame) Cu,Zn-SOD의 분자 계통학적 분석)

  • Kim, Keun-Yong;Nam, Yoon Kwon
    • Korean Journal of Ichthyology
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    • v.18 no.4
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    • pp.293-299
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    • 2006
  • Copper,zinc superoxide dismutase (Cu,Zn-SOD) plays a key role to the first antioxidant defense system against oxidative stress in diverse aerobic organisms. Due to the housekeeping action of Cu,Zn-SOD, it was reported that the structure and function have been conserved during evolution. In this study Cu,Zn-SOD from cloudy catshark Scyliorhinus torazame was subjected to phylogenetic analyses to know its evolutionary relationship in the vertebrate lineage. Molecular phylogenetic trees inferred by NJ, MP, ML and/or Bayesian analyses showed two shark species, Prionace glauca and S. torazame grouped together with high statistical supports. In general, they placed at the separated position from bony vertebrates. Thereafter, bony vertebrates composed of teleosts and birds/mammals (amniotes) formed a monophyletic group. Each teleost and amniote clade was also supported by relatively high statistical values. These phylogenetic relationships are well congruent with the phylogenetic hypothesis of the ancestral position of cartilaginous fishes to bony vertebrates.