• Design & Manufacturing
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• v.13 no.2
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• pp.30-36
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• 2019

Traditional cell culture(2-dimensional) is the method that provide a nutrient and environment on a flat surface to cultivate cells into a single layer. Since the cell characteristics of 2D culture method is different from the characteristics of the cells cultured in the body, attempts to cultivate the cells in an environment similar to the body environment are actively proceeding in the industry, academy, and research institutes. In this study, we will develop a technology to fabricate micro-structures capable of culturing cells on surfaces with various curvatures, surface shapes, and characteristics. In order to fabricate the hemispheric plastic structure(thickness $50{\mu}m$), plastic preform mold (hereinafter as "preform mold") corresponding to the hemisphere was first prepared by injection molding in order to fabricate a two - layer structure to be combined with a flat plastic film. Then, thermoplastic polymer dissolved in an organic solvent was solidified on a preform mold. As a preliminary study, we proposed injection molding conditions that can minimize X/Y/Z axis deflection value. The effects of the following conditions on the preform mold were analyzed through injection molding CAE, [(1) coolant inlet temperature, (2) injection time, (3) packing pressure, (4) volume-pressure (V/P). As a result, the injection molding process conditions (cooling water inlet temperature, injection time, holding pressure condition (V / P conversion point and holding pressure size)) which can minimize the deformation amount of the preform mold were derived through CAE without applying the experimental design method. Also, the derived injection molding process conditions were applied during actual injection molding and the degree of deformation of the formed preform mold was compared with the analysis results. It is expected that plastic film having various shapes in addition to hemispherical shape using the preform mold produced through this study will be useful for the molding preform molding technology and cast molding technology.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.9
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• pp.517-524
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• 2019

The overdrive hub clutch is attached to a 6-speed automatic transmission to reduce fuel consumption by using the additional power of the engine. This paper proposes a means to minimize the load and roll-over ratio on the punch during the piercing process for the overdrive hub clutch product. Die clearance, shear angle, and friction coefficient, which can affect the load and roll-over ratio of the punch during processing, were set as the design variables. Sensitivity analysis was also conducted to determine the influence of each design variable on the punch load and roll-over ratio. As a result, shear angle, friction coefficient and die clearance were found to be sensitive to load and roll-over ratio. The punch load and roll-over ratio were set as the objective function and the equation of each design variable and objective function was derives using the Response Surface Method. Finally, the optimal value of the design variables was derived using the Response Surface Method. Application of this model to finite element analysis resulted in 22.14% improvement in the roll-over ratio of the punch load and material.

• Journal of Life Science
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• v.29 no.10
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• pp.1126-1135
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• 2019

From a sample of bamboo byproduct, the protease-producing yeast strain CO-1 was newly isolated. Strain CO-1 is spherical to ovoid in shape and measures $3.1-4.0{\times}3.8-4.4{\mu}m$. For the growth of strain CO-1, the optimal temperature and initial pH were $30^{\circ}C$ and 4.0, respectively. The strain was able to grow in 0.0-15.0%(w/v) NaCl and 0.0-9.0%(v/v) ethanol. Based on a phylogenetic analysis of its 18S rDNA sequences, strain CO-1 was identified as Pichia anomala. The extracellular protease produced by P. anomala CO-1 was partially purified by ammonium sulfate precipitation, which resulted in a 14.6-fold purification and a yield of 7.2%. The molecular mass of the protease was recorded as approximately 30 kDa via zymogram. The protease activity reached its maximum when 1.0%(w/v) CMC was used as the carbon source, 1.0%(w/v) yeast extract was used as the nitrogen source, and 0.3%(w/v) $MnSO_4$ was used as the mineral source. The protease revealed the highest activity at pH 7.0 and $30^{\circ}C$. This enzyme maintained more than 75% of its stability at a pH range of 4.0-10.0. After heating at $65^{\circ}C$ for 1 hr, the neutral protease registered at 60% of its original activity. The protease production coincided with growth and attained a maximal level during the post-exponential phase.

• Journal of Korean Tunnelling and Underground Space Association
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• v.21 no.3
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• pp.363-375
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• 2019

Since the double-deck tunnel is deeply constructed in the city, it is necessary to secure the installation space of air supply and exhaust, escape passage stairs, elevator, distribution facilities and connection tunnels in the vertical shaft for the double-deck tunnel. Also, in order to minimize the effect of construction on adjacent area, it is necessary to construct the concrete structures at high speed in vertical shaft after tunnel excavation. Therefore, the slabs and the stairs in vertical shaft are needed to be constructed using precast concrete, and the rapid construction techniques of bracket for supporting the inner precast structure are needed. The bracket installation methods include cast-in-place concrete, precast concrete and steel. In this study, the improvement of the steel brackets with good economical efficiency and good workability was carried out in consideration of the improvement of the construction speed. We have developed a new bracket that is optimized through bracket shape improvement, anchor bolt position adjustment and quantity optimization. As a result of the structural performance test, it was confirmed that the required load supporting capacity was secured. As a result of structural performance test for bar type anchor bolt and bent anchor anchor bolt, it was confirmed that the required load carrying capacity was secured and that the load bearing capacity of bent anchor bolt was large.

• The Journal of Korean Institute of Next Generation Computing
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• v.15 no.5
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• pp.64-74
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• 2019

Although a non-rigid registration has high demands in clinical practice, it has a high computational complexity and it is very difficult for ensuring the accuracy and robustness of registration. This study proposes a method of applying a non-rigid registration to 3D magnetic resonance images of brain in an unsupervised learning environment by using a deep-learning network. A feature vector between two images is produced through the network by receiving both images from two different patients as inputs and it transforms the target image to match the source image by creating a displacement vector field. The network is designed based on a U-Net shape so that feature vectors that consider all global and local differences between two images can be constructed when performing the registration. As a regularization term is added to a loss function, a transformation result similar to that of a real brain movement can be obtained after the application of trilinear interpolation. This method enables a non-rigid registration with a single-pass deformation by only receiving two arbitrary images as inputs through an unsupervised learning. Therefore, it can perform faster than other non-learning-based registration methods that require iterative optimization processes. Our experiment was performed with 3D magnetic resonance images of 50 human brains, and the measurement result of the dice similarity coefficient confirmed an approximately 16% similarity improvement by using our method after the registration. It also showed a similar performance compared with the non-learning-based method, with about 10,000 times speed increase. The proposed method can be used for non-rigid registration of various kinds of medical image data.

• Korean Journal of Food Science and Technology
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• v.53 no.2
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• pp.174-180
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• 2021

The optimal recipe for manufacturing composite honey-based herbal pills mainly comprising Rubus coreanus powder (RCP), black mulberry powder (BMP), and vitamin C was investigated. Honey-based herbal pills were prepared by mixing these powders, binding them with honey, and then forming a round shape. The experiment was designed based on the D-optimal mixture design, which included 12 experimental points with one replicate for three independent variables as follows: RCP (10~35%), BMP (10~35%), and vitamin C (5~10%). In addition, the dependent variables (total phenolic and flavonoid content and antioxidant activity) were measured and used to optimize the manufacturing conditions. The results showed that high amounts of RCP were correlated with high total flavonoid content, whereas the addition of high amounts of vitamin C resulted in higher antioxidant activity. In conclusion, an optimized formulation for the honey-based herbal pill was found to contain 35% RCP, 10% BMP, and 10% vitamin C.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.4
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• pp.371-382
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• 2020

There have been continuous attempts to quantify sensory attributes of cosmetic products by measuring relevant physical properties. The most representative method to evaluate stickiness is to measure axial force using texture analyzer. Stickiness is known to correlate with AUC which abbreviates area under curve in the obtained axial force curve as a function of time. Recently, Normandie University research group developed in vivo stickiness evaluation method considering the characteristics of skin along with established evaluation method[8]. Based on the study, we tried to optimize in vivo stickiness evaluation method especially for cosmetic creams. The experiment was carried out on 5 different facial creams products by changing the amount and the times of rolling of creams, and the shape and material of probes. Based on the results of the sensory evaluation, the most consistent conditions were established as the optimal evaluation method. As a result, applying 70 μL of cream and rubbing 10 times for 7 s inside the 3.4 cm circle were judged to be suitable. As for the probes, spherical metallic probe was more proper due to its reproducibility. We conducted the settled method on 10 subjects to check its validity. Although the absolute values of AUC differed depending on the individuals, the AUC values were all ranked the same. Finally, for the standardization of stickiness of AUC, polyvinylpyrrolidone (PVP) was set as a reference material and we measured AUC of its aqueous solution by changing concentration. Then, the degree of stickiness recognition for 5 different creams was surveyed to check the correlation between AUC and stickiness.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.36 no.4
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• pp.213-220
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• 2023

This paper presents a model simulating the folding process and collision dynamics of "ddakji", a traditional Korean game played using paper tiles (which are also referred to as ddakji). The model uses two A4 sheets as the base materials for ddakji. The folding process involves a series of boundary conditions that transform the wing part of the paper structure into a twisted configuration. A rigid plate boundary condition is also adopted for squeezing, establishing the shape and stress state of the game-ready ddakji through dynamic relaxation analysis. The gaming process analysis involves a forced displacement of the striking ddakji to a predetermined collision position. Collision analysis then follows at a given speed, with the objective of overturning the struck ddakji--a winning condition. A genetic algorithm-based optimization analysis identifies the optimal collision conditions that result in the overturning of the struck ddakji. For efficiency, the collision analysis is divided into two stages, with the second stage carried out only if the first stage predicts a possible overturn. The fitness function for the genetic algorithm during the first stage is the direction cosine of the struck ddakji, whereas in the second stage, it is the inverse of the speed, thus targeting the lowest overall collision speed. Consequently, this analysis provides optimal collision conditions for various compression thicknesses.

• Journal of the Korean Geosynthetics Society
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• v.22 no.1
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• pp.53-66
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• 2023

In this study, the results of the elasto-plastic beam analysis, finite element analysis and optimization design of the steel pipe sheet pile applied as an earth retaining wall under the deep excavation were presented. Through this study, it was found that the high-strength and sea resistant steel pipe has high allowable stress, excellent structural properties, favorable corrosion, and high utilization as an earth retaining wall, and the C-Y type joint has significantly improved the tensile strength and stiffness compared to the traditional P-P type. In addition, it was investigated that even if the leak or defect of the wall occurs during construction, it has the advantage of being able to be repaired reliably through welding and overlapping. In the case of steel pipe wall, they were evaluated as the best in views of the deep excavation due to the large allowable bending stress and deformation flexibility for the same horizontal displacement than CIP or slurry wall. Elasto-plastic and finite element analysis were conducted in consideration of ground excavation under large-scale earth pressure (uneven pressure), and the results were compared with each other. Quantitative maximum value were found to be similar between the two methods for each item, such as excavation behavior, wall displacement, or member force, and both analysis method were found to be applicable in design for steel pipe sheet pile wall. Finally, it was found that economical design was possible when determining the thinnest filling method with concrete rather than the thickest hollow shape in the same diameter, and the depth (the embedded length through normality evaluation) without rapidly change in displacement and member force.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.33 no.6
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• pp.288-293
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• 2023

To optimize the process parameters of laser powder bed fusion process to fabricate the high density STS316L alloy, the effect of laser power, scanning speed and hatching distance on the relative density was studied. Tensile properties of additively manufactured STS316L alloy using optimized parameters was also evaluated according to the build direction. As a result of additive manufacturing process under the energy density of 55.6 J/mm³, 83.3 J/mm³ and 111.1 J/mm³, high density STS316L specimens was suitably fabricated when the energy density, power and scan speed were 83.3 J/mm³, 225 W and 1000 mm/s, respectively. The yield strength, ultimate tensile strength, and elongation of STS316L specimens in direction perpendicular to the build direction, show the most competitive values. Anisotropic shape of the pores and the lack of fusion defects probably caused strain localization which result in deterioration of tensile properties.