• Title/Summary/Keyword: shape formation

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The Oogenesis of Chinese minnow, Leuciscinae, Teleostei (경골어류 황어아과 버들치의 난자형성과정)

  • Kim, Dong-Heui;Chang, Byung-Soo;Jung, Han-Suk;Teng, Yung-Chien;Kim, Seok;Lee, Kyu-Jae
    • Applied Microscopy
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    • v.39 no.3
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    • pp.237-243
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    • 2009
  • Chinese minnow, Rhynchocypris oxycephalus is a teleost belonging to Leuciscinae, Cyprinidae. The oogenesis and ultrastructure of egg envelope in Chinese minnow were investigated by light and electron microscopes. The ovary was of white yellowish and ellipsoidal shape with the major axis 30 mm and the minor axis 7mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. The outer surface of egg envelope was covered by microvilli-structures, and had a micropyle on the area of animal pole. Egg envelope consisted with 2 layers, an adhesive outer layer with microvilli-structures and fibrillar inner layer. In conclusion, the oogenesis of Chinese minnow was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilic substance in the cytoplasm. The oogenesis of Chinese minnow seems to share common patterns in Cyprinidae, but these ultrastructural unique characters of egg envelope can be utilized in taxonomy of teleost.

ELECTRON MICROSCOPIC STUDY OF RESIN-DENTIN HYBRID ZONE PRODUCED BY THE MOISTENING OF ACID CONDITIONED DENTIN SURFACE (산 표면처리 후 상아질 표면의 습윤이 하이브리드층 형성에 관한 전자현미경적 연구)

  • Lee, Kwang-Won;Lee, Myung-Jong
    • Restorative Dentistry and Endodontics
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    • v.20 no.2
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    • pp.463-486
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    • 1995
  • The effect of moistening and air-drying of acid-conditioned dentin before priming on the formation of resin-dentin hybrid zone was investigated, Freshly extracted human molars were used and divided at random into 5 groups, Groups 1 - 3 consisted of specimens conditioned with 10 % phosphoric acid for 20 seconds; Group 1 served as a control in which the conditioned dentin was simply blot-dried with a damp facial tissue; Group 2 was air dried for 30 seconds ; Group 3 was air dried for 30 seconds and immediately remoistened for 10 seconds with air-water syringe. and then the specimen was blot-dried with a damp facial tissue. Groups 4-5 were not acid conditioned ; In group 4, the smear layer on the dentin was blot dried before primer placement; Group 5 was air dried only for 30 seconds, The acetone-based primer and bonding agent of All Bond 2 (Bisco. Inc., USA) and composite resin (Z-100, 3M Dental products, USA) were applied for acid conditioned dentin and non-conditioned dentin. The morphologic ultrastructure of resin-dentin hybrid zone was examined by the use of SEM and TEM. and the existence of inorganic material and analysis of Ca/P weight-percent ratio in the resin-dentin hybrid zone were revealed by the EDAX, The results were as follows : 1. In the moistened specimens from acid-conditioned groups, the resin penetrated about 3-$4{\mu}m$ into dentin and the denatured collagen smear layer was not present at the surface. The resin tag was formed to a thickeness of 3-$4{\mu}m$ at the upper part of dentinal tubule and compactively connected to each other by means of many lateral branching. 2. In the air-dried specimens from acid-conditioned groups, the resin penetrated about 2.0-$2.5\;{\mu}m$ into dentin and an upper thin black layer to a thickness of 30-35nm was identified between adhesive resin and demineralized collagen layer. The resin tag to have a diameter of $2.5{\mu}m$ was formed at the upper part of dentinal tubule. However the funnel shape of the tag was not notable compared to the moistened specimens. 3. In the remoistened specimens from acid conditioned groups, the resin penetrated about 2.0-$2.5{\mu}m$ into dentin and an upper black layer was not present. The resin tag at the upper part of dentinal tubule was formed less than $2{\mu}m$ and was weakly connected to each other by means of few lateral branching. 4. In the non-conditioned groups, the smear layer was formed to a thickness of $0.5{\mu}m$ at dentin surface. However, the resin-dentin hybrid zone was not identified by TEM. The evidence of resin penetration into intertubular and intratubular dentin did not show. 5. All the acid-conditioned groups showed that the detected calcium and phosphorus weight percent ratios at the $2{\mu}m$ upper portion from the resin-dentin interface into the resin were much higher than that at the $2{\mu}m$ lower portion from the resin-dentin interface to dentin. (P<0.01).

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The Effect of Pore Sizes on Poly(L-lactide-co-glycolide) Scaffolds for Annulus Fibrosus Tissue Regeneration (조직공학적 섬유륜재생을 위한 PLGA 지지체 제조시 다공크기의 영향에 관한 연구)

  • So, Jeong-Won;Jang, Ji-Wook;Kim, Soon-Hee;Choi, Jin-Hee;Rhee, John-M.;Min, Byung-Hyun;Khang, Gil-Son
    • Polymer(Korea)
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    • v.32 no.6
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    • pp.516-522
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    • 2008
  • Biodegradable polymers have been used extensively as scaffolding materials to regenerate new tissues and the ingrowth of tissue have been reported to be dependent directly of the porosity, pore diameter, pore shape, and porous structure of the scaffold. In this study, porous poly (L-lactide-co-glycolide) (PLGA) scaffolds with five different pore sizes were fabricated to investigate the effect of pore sizes for AF tissue regeneration. Cellular viability and proliferation were assayed by MTT test. Hydroxyproline/DNA content of AF cells on each scaffold was measured. sGAG analyses were performed at each time point of 2 and 6 weeks. Scaffold seeded AF cells were implanted into the back of athymic nude mouse to observe the difference of formation of disc-like tissue depending on pore size in vivo. We confirmed that scaffold with $180{\sim}250{\mu}m$ pores displayed high cell viability in vitro and produced higher ECM than scaffold with other pore sizes in vivo.

GIS spatial D/B formation of geothermal data and Distribution of Heat Flow of Korea (한국의 지열자료 GIS 공간 D/B 구축과 지열류량 분포)

  • Kim, Hyoung-Chan;Lee, Young-Min;Park, Jeong-Min
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.06a
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    • pp.459-460
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    • 2006
  • 현재 남한의 지열류량 측정값으로는 총 363개 지점의 자료가 측정 및 수집되어 있다. 이것은 Mizutani et at. (1970), 장정진 외(1970), 그리고 서정희(1976) 등의 자료, 총 35개의 자료도 추가된 것이다. 1989년 이후부터 측정된 지열류량 자료는 217개 자료이며(임정웅 외, 1989; 임정웅 외, 1996; Lim and Kim, 1997; 염병우 외, 1997), 모두 직접 측정한 것이나, 1989년 이후 보고된 지열류량 자료에 약간의 오류가 있어 이번 연구에서 수정 보완하였다. 또한 과거의 자료 35개 자료는 이미 지열류량 측정치가 논문화 되어 있는 것으로 암석시료는 없다 1989년 이후 2004년까지 자료 217개 2005년도 추가 자료 111개의 지열류량 자료는 암석시료도 있으며, 측정기기가 서로 달라 오차가 있을 수 있어 서로 보정을 해야 할 필요가 있어 시추공 주변 암석을 새로 수집해서 신장비로 다시 측정 보정하였다. 지열류량 D/B 구축은 각 자료의 일련번호, 고유번호 (Sn.), 위경도 좌표 (longitude, lattitude), 암석의 열전도도(thermal conductivity), 지온경사 (thermal gradient), 지열류량 (heat flow)등으로 구성되어 있다. 지열류량 자료 공간 데이터베이스는 점 속성을 가지며 자료형태는 각종 소프트웨어와 호환성이 좋은 shape 파일 형태로 작성하였다. 또만 최근 천부 토양 및 암석 열물성을 이용한 냉난방시스템 즉, Heat Pump System 설계를 위하여 반드시 들어가야 하는 요소인 열확산율, 공극율, 밀도, 비열 등 열물성 특성을 추가하여 GIS 공간 D/B구축하였다. 대륙붕 자료 4개 자료를 제외하고 359개의 지열류량 자료를 이용하여 한반도 남부, 즉 남한의 지열류량 분포도를 작성 분석해 본 결과(그림 1), 우리나라의 지열류량 이상대는 아산만 주변, 보령, 유성, 진안, 울진, 포항, 부산 지역과 포천, 속초, 충주, 수안보 등 지역에서 나타난다 이러한 이상대 주변에는 대개 온천이 발달되어 있었거나 새로 개발되어 있는 곳이다. 온천에 이용하고 있는 시추공의 자료는 배제하였으나 온천이응으로 직접적으로 영향을 받지 않은 시추공의 자료는 사용하였다 이러한 온천 주변 지역이라 하더라도 실제는 온천의 pumping 으로 인한 대류현상으로 주변 일대의 온도를 올려놓았기 때문에 비교적 높은 지열류량 값을 보인다. 한편 한반도 남동부 일대는 이번 추가된 자료에 의해 새로운 지열류량 분포 변화가 나타났다 강원 북부 오색온천지역 부근에서 높은 지열류량 분포를 보이며 또한 우리나라 대단층 중의 하나인 양산단층과 같은 방향으로 발달한 밀양단층, 모량단층, 동래단층 등 주변부로 NNE-SSW 방향의 지열류량 이상대가 발달한다. 이것으로 볼 때 지열류량은 지질구조와 무관하지 않음을 파악할 수 있다. 특히 이러한 단층대 주변은 지열수의 순환이 깊은 심도까지 가능하므로 이러한 대류현상으로 지표부근까지 높은 지온 전달이 되어 나타나는 것으로 판단된다.

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Breeding of Phalaenopsis 'SM 333' with Mini Multiple Flower Formation (소형 다화 분지성 호접란 'SM 333' 육성)

  • Park, No Eun;Son, Beung Gu;Kim, Hong Yul;Lim, Ki-Byung
    • Horticultural Science & Technology
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    • v.33 no.1
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    • pp.149-154
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    • 2015
  • A new Phalaenopsis cultivar 'SM 333' was bred by Sangmiwon Orchid, Korea, which produces young plants through tissue culture techniques. The new cultivar 'SM 333', showing the phenotype of multiflora with pink color and small, multibranching-type characteristics, was derived from crossing between Phalaenopsis 'Odoriko' and 'Be Tris'. An elite individual number '02-03-33' later termed 'SM 333' was selected among about 300 individual progenies, based on an intensive selection process covering vegetative and flowering distinctiveness over more than 2 years. In year 2004-2005, the 1st and 2nd characteristic analyses were carried out through performance and uniformity tests. 'SM 333' shows flower color that is bright clean pink (RHS # RP69D) and flower shape that is formal type with 5.0 and 5.8 cm in flower height and width, respectively. 'SM 333' is regarded as raceme flower type suitable for the small casual flower market. The leaves of 'SM 333' grow horizontally and about 20.8 cm in length and 6.5 cm in width. This cultivar also possesses no genetic variation, and is amenable to fast in vitro propagation and easy growth due to its vigorous growth habit. This 'SM333' was registered (Reg. # 2916) with Korea Seed & Variety Service (KSVS) on 1st December, 2009, and the plant breeder's right is currently controlled by Sangmiwon Orchid Company, Korea.

Fractal Analysis of Tidal Channel using High Resolution Satellite Image (고해상도 위성 영상을 이용한 조류로의 프랙털 분석)

  • Eom, Jin-Ah;Lee, Yoon-Kyung;Ryu, Joo-Hyung;Won, Joong-Sun
    • Korean Journal of Remote Sensing
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    • v.23 no.6
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    • pp.567-573
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    • 2007
  • Tidal channel development is influenced by sediment type, grain size, composition and tidal current. Tidal channels are usually characterized by channel formation, density and shape. Quantitative analysis of tidal channels using remotely sensed data have rarely been studied. The objective of this study is to quantify tidal channels in terms of fractal dimension and compare different inter-tidal channel patterns and compare with DEM (Digital Elevation Model). For the fractal analysis, we used box counting method which had been successfully applied to streams, coastlines and others linear features. For a study, the southern part of Ganghwado tidal flats was selected which know for high dynamics of tidal currents and vast tidal flats. This area has different widths and lengths of tidal channels. IKONOS was used for extracting tidal channels, and the box counting method was applied to obtain fractal dimensions (D) for each tidal channel. Yeochari area where channels showed less dense development and low DEM had low fractal dimenwion near $1.00{\sim}1.20$. Area (near Donggumdo and Yeongjongdo) of dendritic channel pattern and high DEM resulted in high fractal dimension near $1.20{\sim}1.35$. The difference of fractal dimensions according to channel development in tidal flats is relatively large enough to use as an index for tidal channel classification. Therefore we could conclude that fractal dimension, channel development and DEM in tidal channel has high correlation. Using fractal dimension, channel development and DEM, it would be possible to quantify the tidal channel development in association with surface characteristics.

Analysis of Rock Slope Behavior Utilizing the Maximum Dip Vector of Discontinuity Plane (불연속면의 최대경사벡터를 활용한 사면거동해석)

  • Cho, Taechin
    • Tunnel and Underground Space
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    • v.29 no.5
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    • pp.332-345
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    • 2019
  • Maximum dip vector of individual joint plane, which can be uniquely defined on the hemispherical projection plane, has been established by considering its dip and dip direction. A new stereographic projection method for the rock slope analysis which employs the maximum dip vector can intuitively predict the failure modes of rock slope. Since the maximum dip vector is uniquely projected on the maximum dip point of the great circle, the sliding direction of discontinuity plane can be recognized directly. By utilizing the maximum dip vector of discontinuity both the plane sliding and toppling directions of corresponding blocks can be discerned intuitively. Especially, by allocating the area of high dip maximum dip vector which can form the flanks of sliding block the potentiality for the formation of virtual sliding block has been estimated. Also, the potentiality of forming the triangular-sectioned sliding block has been determined by considering the dip angle of joint plane the dip direction of which is nearly opposite to that of the slope face. Safety factors of the different-shaped blocks of triangular section has been estimated and compared to the safety factor of the most hazardous block of rectangular section. For the wedge analysis the direction of crossline of two intersecting joint planes, which has same attribute of the maximum dip vector, is used so that wedge failures zone can be superimposed on the stereographic projection surface in which plane and toppling failure areas are already lineated. In addition the maximum dip vector zone of wedge top face has been delineated to extract the wedge top face-forming joint planes the orientation of which provides the vital information for the analysis of mechanical behavior of wedge block.

Thermotropic Liquid Crystalline Behavior of Poly[1-{4-(4'-nitrophenylazo)phenoxycarbonylalkanoyloxy}ethylene]s (폴리[1-{4-(4'-니트로페닐아조)페녹시카보닐알카노일옥시}에틸렌]들의 열방성 액정 거동)

  • Jeong, Seung-Yong;Ma, Yung-Dae
    • Polymer(Korea)
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    • v.32 no.5
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    • pp.489-496
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    • 2008
  • The thermotropic liquid crystalline behavior of a homologous series of poly[1-{4-(4' nitrophenylazo) phenoxycarbonylalkanoyloxy}ethylene]s (NAPEn, n = $2{\sim}8$,10, the number of methylene units in the spacer) have been investigated. All of the homologues formed monotropic nematic phases. The glass transition temperatures decreased with n. This is attributed to a plasticization of the backbone by the side chains. The isotropic-nematic phase transition temperatures decreased with increasing n up to 7 and showed the odd-even effect. However it became almost constant when n is more than 7. This behavior was rationalized in terms of the change in the average shape of the side chain on varing the parity of the spacer. This rationalization also accounts for the observed variation of the entropic gain for the clearing transition. The mesophase properties of NAPEn were entirely different from those reported for the polymers in which the azobenzene groups are attached to polyacrylate, polymathacrylate, and polystyrene backbones through polymethylene spacers. The results indicate that the mode of chemical linkage of the side group with the main chain plays an important role in the formation, stabilization, and type of mesophase.

Stabilization of Tocopheryl Acetate of Swollen Micelle by Poloxamer (Poloxamer를 이용한 Swollen Micelle의 Tocopheryl Acetate 안정화)

  • Kim, Mi-Seon;Yoon, Kyung-Sup
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.2
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    • pp.609-622
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    • 2019
  • When the surfactant is dissolved in an aqueous solution, it forms aggregate called micelles (<20 nm) in the solution, and micelles can form the solubilized formulation by supporting the active ingredient therein. Swollen micelles are formulations capable of carrying larger amounts of active ingredient than conventional solubilized formulations at 50~100 nm. Unlike liposomes or nanoemulsions, which require a separate process such as high pressure emulsification, Swollen micelle is a more efficient method of solubilization and particle formation from a productive point of view. In this study, stabilization experiments on swollen micelle formulations were carried out using poloxamer 407, and then optimized formulation experiments for tocopheryl acetate components were performed using Response Surface Methodology (RSM). Tocopheryl acetate, a surfactant that affects solubilization and an active substance, were set as a factor and the correlation between them was confirmed. As the evaluation method, stability and particle size distribution and size were confirmed by temperature and time, and the structure and shape of the swollen micelle carrying the active ingredient were confirmed by FIB. These results show that poloxamer 407 0.500%, octyldodeceth-16 0.387% and tocopheryl acetate 0.945% are the most optimized prescriptions for swollen micelle stabilized with tocopheryl acetate.

Carbon diffusion behavior and mechanical properties of carbon-doped TiZrN coatings by laser carburization (레이저 침탄된 TiZrN 코팅에서 탄소확산거동과 기계적 특성)

  • Yoo, Hyunjo;Kim, Taewoo;Kim, Seonghoon;Jo, Ilguk;Lee, Heesoo
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.31 no.1
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    • pp.32-36
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    • 2021
  • This study was investigated in carbon diffusion behavior of laser-carburized TiZrN coating layer and the changes of mechanical properties. The carbon paste was deposited on TiZrN coatings, and the laser was irradiated to carburize into the coatings. The XRD peak corresponding to the (111) plane shifted to a lower angle after the carburization, showing the lattice expansion by doped carbon. The decreased grain size implied the compression by the grain boundary diffusion of carbon. The XPS spectra for the bonding states of carbon was analyzed that carbon was substitute to nitrogen atoms in TiZrN, as carbide, through the thermal energy of laser. In addition, the combination of sp2 and sp3 hybridized bonds represented the formation of an amorphous carbon. The cross-sectional TEM image and the inverse FFT of the TiZrN coating after carburizing were observed as the wavy shape, confirming the amorphous phase located in grain boundaries. After the carburization, the hardness increased from 34.57 GPa to 38.24 GPa, and the friction coefficient decreased by 83 %. In particular, the ratio of hardness and elastic modulus (H/E) which is used as an index of the elastic recovery, increased from 0.11 to 0.15 and the wear rate improved by 65 %.