• Journal of East-Asian Urban History
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• v.2 no.1
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• pp.27-63
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• 2020

The emergence of coffee shops and other public spaces in modern Shanghai shows the appearance of the "mass" centered on the middle class. Furthermore, we can further explore the different development paths of the publicity of modern Chinese urban society. The emergence of new public leisure spaces, such as cafes, provides a model of modern life style and a stage of daily publicity for the middle class in Shanghai. With the convenience provided by this kind of public space, people are able to clean up their old opinions and be better at accepting new ideas. A more sensitive and flexible public opinion of Enlightenment has gradually formed in urban life.

• Journal of Microbiology and Biotechnology
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• v.20 no.4
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• pp.678-682
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• 2010

The deletion of sti from the Streptomyces plasmid pIJ101 made its derivative pHZ1358 an efficient vector for gene disruption and replacement. Here, pHZ1358 was further optimized by the construction of a derivative plasmid pJTU1278, in which a cassette carrying multiple cloning sites and a lacZ selection marker were introduced for convenient plasmid construction in E. coli. In addition, the oriT region of pJTU1278 was also deleted, generating a vector (pJTU1289) that can be used specifically for PCR-targeting. The efficient usage of these vectors was demonstrated by the deletion of the gene involved in avermectin biosynthesis in S. avermitilis.

• Journal of Navigation and Port Research
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• v.34 no.7
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• pp.567-575
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• 2010

Ningbo Port develops quickly in recent years and with the combination of Ningbo Port and Zhoushan Port, it becomes another important port in the Shanghai International Shipping center. Competition between the two biggest ports in this area can't be avoided. The goal of this paper is investigating the competence of port from two angles, which are spatial structure of the ports cluster and neighboring ports' attraction to cargoes at conjunct hinterlands. The paper firstly uses the HHI index model and shift-share method to qualitatively and quantitatively analyze the data of container throughputs of ports in the shipping center, in order that investigates the spatial structure of ports cluster. Basing on above researches, the paper employs the location quotient to study Shanghai port and Ningbo port's hinterland distributions at Zhejiang province. The conclusion of this study:(I)the ports cluster of the Shanghai International Shipping Center is highly centralized, and undergoes a porcess of first centralization then decentralization since the mid of 90s, last century. (2)Hinterlands of Shanghai port includes: Hangzhou, Jiaxing, Huzhou and Quzhou districts; Hinterlands of Ningbo port includes: Ningbo, Taizhou and Zhoushan districts.

• Proceedings of the KOR-KST Conference
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• 1995.05a
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• pp.51-59
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• 1995

It is an objective of Shanghai urban development to quickly build Shanghai into one of the international centres of economy, finance and trade. To fulfil the objective, a rational urban general layout and a satisfactory traffic network are needed. As a metropolis with complete urban functions and specified geographical position, Shanghai must develop a perfect citytown system that best suits its composite urban distribution. In planning the central city, the principle of "open and multi-centred" and "optimization of land use" should be taken into consideration. To build a satisfactory urban traffic network, emphasis should be laid upon the construction of deefwater wharf, air-field and inforation centre. In addition, determination should be made to build a high-speed traffic means including high speed railroad and express highway so that a public traffic network is realised on and above ground, and underground. A solution of traffic preblem in Shanghai lies in good understanding of traffic policy and strenthening of strategic management combined with a rational layout of traffic circulation.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.10
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• pp.4245-4250
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• 2014

Emerging evidence has suggested that glycolysis is enhanced in cancer-associated fibroblasts (CAF), and miR-186 is downregulated during the CAF formation. However, it is not clear whether miR-186 is involved in the regulation of glycolysis and what the role of miR-186 plays during the CAF formation. In this study, quantitative PCR analysises show miR-186 is downregulated during the CAF formation. Moreover, miR-186 targets the 3' UTR of Glut1, and its overexpression results in the degradation of Glut1 mRNA, which eventually reduces the level of Glut1 protein. On the other hand, knockdown of miR-186 increased the expression of Glut1. Both time course and dose response experiments also demonstrated that the protein and mRNA levels of Glut1 increase during CAF formation, according to Western blot and quantitative PCR analyses, respectively. Most importantly, besides the regulation on cell cycle progression, miR-186 regulates glucose uptake and lactate production which is mediated by Glut1. These observations suggest that miR-186 plays important roles in glycolysis regulation as well as cell cycle checkpoint activation.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.1
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• pp.127-131
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• 2013

Trastuzumab is the first molecular targeting drug to increase the overall survival rate in advanced gastric cancer. However, it has also been found that a high intrinsic or primary trastuzumab resistance exists in some proportion of gastric cancer patients. In order to explore the mechanism of resistance to trastuzumab, firstly we investigated the expression of MUC1 (membrane-type mucin 1) in gastric cancer cells and its relationship with drug-resistance. Then using gene-silencing, we transfected a siRNA of MUC1 into drug-resistant cells. The results showed the MKN45 gastric cell line to be resistant to trastuzumab, mRNA and protein expression of MUC1 being significantly upregulated. After transfection of MUC1 siRNA, protein expression of MUC1 in MKN45cells was significantly reduced. Compared with the junk transfection and blank control groups, the sensitivity to trastuzumab under MUC1 siRNA conditions was significantly increased. These results imply that HER2-positive gastric cancer cell MKN45 is resistant to trastuzumab and this resistance can be cancelled by silencing expression of the MUC1 gene.

• Journal of the Korean Society of Clothing and Textiles
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• v.29 no.1 s.139
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• pp.124-135
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• 2005

This study was intended to compare and analyze the clothing purchasing behavior of Chinese career women in Benjing, Shanghai, Shenzhen, and Changchun, and to provide useful information for establishment of marketing strategies for China. The subjects were 662 career women in their 20's or 30's living in Benjing, Shanghai, Shenzhen, and Changchun. Data was collected through the questionnaire based on the theoretical and previous studies from April 9, 2003 to April 31, 2003. A total of 662 questionnaires was analyzed by frequency, percentage, ANOVA, Duncan's multiple range test, and ${\chi}^2-test$. The results of this study were as follows: 1. Demographic variables such as an age, marriage, academic back ground, residential city, monthly income, and monthly expenditure on clothing were significantly different among the career women in Benjing, Shanghai, Shenzhen, and Changchun. 2. Clothing purchasing behavior variables such as clothing purchasing motive, using informants, clothing selection standards, store selection standards, and satisfaction after purchasing clothes were significantly different among the career women in Benjing, Shanghai, Shenzhen, and Changchun.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.3
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• pp.1363-1376
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• 2020

An improved clustering scheme based on user group is proposed. Every two users are grouped among N-users in the allowed system according to their link gain from large to small. Each user group is numbered sequentially. Two user clusters are obtained according to the principle of maximizing link gain difference for the users in the first and last user groups. The remaining user groups are added to the two existing user clusters according to the parity of the group number. The clustering should be clustered again among the users in either user cluster if the throughput summation of a user cluster in NOMA is less than that of these users in orthogonal multiple access. The simulation results show that the proposed clustering scheme can increase the system throughput by about 8% compared with the hybrid clustering scheme when the number of users requiring service is 12.

• Food Science of Animal Resources
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• v.37 no.6
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• pp.847-854
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• 2017

A rapid, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of four barbiturates (phenobarbital, pentobarbital, amobarbital and secobarbital) in raw milk. The barbiturates were extracted using liquid-liquid extraction, ultrasonication and centrifugation, and purified on an SPE column. Analytes were separated by HPLC on a CSH C18 column eluted using an acetonitrile-water system with a linear gradient dilution programme, and detected by MS/MS. The recoveries of the barbiturates were 85.0-113.5%, and the intra- and inter-assay RSDs were less than 9.8% and 7.3%, respectively. The limit of detection was 5 ng/mL for all four of the barbiturates. The analytical method exhibited good linearity from 10 to 1000 ng/mL; the correlation coefficient ($r^2$) was greater than 0.9950 for each barbiturate. This method was also applied to the determination of barbiturates in real milk samples and was found to be suitable for the determination of veterinary drug residues in raw milk.

• Steel and Composite Structures
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• v.22 no.4
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• pp.821-835
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• 2016

The paper presents an experimental study of the structural behavior of circular flyash-concrete-filled steel tubular stub columns under axial compressive loads. In this study, 90% and 100% by weight of the cement in the concrete core was replaced with flyash. Twenty-seven specimens were tested to study the influence of flyash content, wall thickness of the steel tube, and curing age on the ultimate capacity and confinement effect. The experimental results were compared with the design values calculated using AISC-LRFD (1999), ACI (1999), AIJ (1997) and Eurocode 4 (1994). From the experimental study, it was determined that the confinement effect of circular steel tubes filled with high content flyash concrete was better than that of specimens filled with ordinary Portland cement concrete. The 5.88-mm-thick steel tube filled with 100% flyash concrete was equivalent in strength to a steel tube filled with C30 concrete at 28 days.