• Asian-Australasian Journal of Animal Sciences
• /
• 제33권3호
• /
• pp.515-524
• /
• 2020

Objective: Human mesenchymal stromal cells (MSCs) exhibit variable differentiation potential and can be divided accordingly into distinct subpopulations whose ratios vary with donor age. However, it is unknown whether the same is true in pigs. This study investigated MSC subpopulations in miniature pig and compared their characteristics in young (2 to 3 months) and adult (27 to 35 months) pigs. Methods: Osteogenic, chondrogenic, and adipogenic capacity of isolated MSCs was evaluated by von Kossa, Alcian blue, and oil red O staining, respectively. Cell surface antigen expression was determined by flow cytometry. Proliferative capacity was assessed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Expression of marker genes was detected by quantitative real-time polymerase chain reaction. Results: Porcine MSCs comprised cells with trilineage and bilineage differentiation potential (tMSCs and bMSCs, respectively) and non-differentiating stromal cells (NDSCs). The tMSC and bMSC fractions were smaller in adult than in young pigs (63.0% vs 71.2% and 11.6% vs 24.0%, respectively, p<0.05); NDSCs showed the opposite trend (25.4% vs 4.8%; p<0.05). Subpopulations showed no differences in morphology, cell surface antigen expression, or proliferative capacity, but octamer-binding transcription factor 4 (OCT4) expression was higher in tMSCs than in bMSCs and NDSCs (p<0.05), whereas sex determining region Y-box 2 (SOX2) expression was higher in tMSCs and bMSCs than in NDSCs (p<0.05). Aging had no effect on these trends. Conclusion: Porcine MSCs comprise distinct subpopulations that differ in their differentiation potential and OCT4 and SOX2 expression. Aging does not affect the characteristics of each subpopulation but alters their ratios.

• 한국동물학회:뉴스레터
• /
• 제12권2호
• /
• pp.96.2-96
• /
• 1995

No Abstract, See Full Text

• 한국양식학회지
• /
• 제1권1호
• /
• pp.53-66
• /
• 1988

틸라피아는 암수간에 성장차가 커 수컷 종묘만을 생산하여 양식함은 산업적으로 매우 중요시된다. 본 연구는 나일틸라피아의 암컷을 수컷으로 성전환시키기 위한 연구로써 본 종의 초기생식소 분화 및 여러 농도의 호르몬 처리에 의한 성전환율을 구한 후 호르몬이 성장에 미치는 효과를 조사하여 다음과 같은 결과를 얻었다. 나일틸라피아의 초기 생식소는 원생식세포의 점진적인 분열에 의해 부화후 9일째 형성되었으며, 난소와 정소로의 성 분화는 부화후 약 20일부터 조직학적으로 식별되었다. 17$\alpha$-mrthyltestosterone (MT)을 15, 30 및 60ppm의 농도로 10$\~$40일 동안 처리한 후 성전환율을 조사한 결과 모든 실험군에서 $95\%$ 이상의 수컷이 생산되었다. 특히 15ppm-40일, 30ppm-30일과 30ppm-40일 처리군에서는 $100\%$ 수컷이 유도되었다. 호르몬 처리에 의한 성장의 증가는 대조군에 비해 비교적 뚜렷하게 나타났으며, condition factor 역시 크게 나타나 MT는 틸라피아의 성장을 증가시킴은 물론 비만하게 함을 알 수 있었다.

• 한국가금학회지
• /
• 제20권4호
• /
• pp.177-188
• /
• 1993

닭에서 적절한 성감별 방법을 개발하고 닭의 성분화 기작의 기초자료를 얻기 위하여 배아의 섬유아세포의 염색체를 분석하고, W 염색체 특이적인 반복염기서열과 50∼60％의 유사성을 보이는 random primer로 PCR 증폭을 실시하여 성을 판별하는 방법이 이용되었으며, 닭에서 성분화에 관련된 유전자를 분리하기 위해 W 염색체 특이적인 반복염기서열을 클로닝하였고, PCR을 이용하여 ZFY와 SRY 염기서열을 증폭하였다. 닭의 배아섬유세포의 염색체 분석 결과 Z 염색체와 W 염색체를 구분함으로써 배아의 성을 직접적으로 판별하는 것이 가능하였으며 , 암닭의 DNA를 Xho Ⅰ와 Eco RI로 절단하여 생성되는 band를 이용하여 성을 판별하는 것이 가능하였다. Xho Ⅰ와 Eco RI family를 클로닝하고, colony hybridization을 통해 Xho Ⅰ과 염기서열이 유사한 80∼100개의 clone을 동정하여, 이들 두 그룹간 DNA homology는 매우 유사하였다. 150개의 random primer 중 W 염색체 특이적인 반복 염기서열과 유사성을 보이는 primer 7개를 screening하였으며, 이 중 3개의 primer는 닭에서 자성과 웅성간의 차이를 나타내었다. 닭에서 성분화에 관련된 유전자를 동정하기 위하여 포유류의 ZFY와 SRY유전자의 PCR증폭을 실시하였다. ZFY를 증폭한 결과, 자성과 웅성간의 차이를 발견할 수 없었으며, 이는 닭에서 ZFY는 상염색체 또는 Z 염색체에 존재함을 시사한다. SRY의 증폭에서는 성간의 차이가 확인되었으나, 이 유전자가 Z 염색체에 존재하는지 W 염색체에 존재하는지 혹은 상염색체 존재하는지 여부는 연구가 필요하리라 사료된다.

• 가정과삶의질연구
• /
• 제26권2호
• /
• pp.155-168
• /
• 2008

The purpose of this study was to investigate the relationships of parental attachment and self-differentiation with fear of intimacy perceived by college students. The data of the study were collected through self-administered questionnaire method with 187 male and 159 female college students. The major results of this research were as follows. First, there were significant differences in the level of attachment with parents according to parent's marital statue, living standard, and father's education level. Second, there were significant differences in the level of self-differentiation across sex, age, parent's marital statue, living standard, and parental's education level. Third, the level of attachment with parents was positively correlated with self-differentiation, indicating that the greater the level of attachment with parents, the higher the degree of self-differentiation. Fourth, fear of intimacy was negatively associated with parental attachment and self-differentiation, showing that the greater the degree of fear of intimacy, the lower the lever of attachment with parents and self-differentiation. Finally, multiple regression analysis result reported that emotional cutoff among the four self-differentiation subscales was the most powerful variable predicting the fear of intimacy. The results were shortly discussed in terms of some implications for education and counseling with college students.

• 대한가정학회지
• /
• 제43권11호
• /
• pp.31-48
• /
• 2005

The purpose of this study was to explore the direct and indirect effects of premarital adult child's perceived self-differentiation and parent-child dynamics of emotional system on trait anxiety. The subjects of the study numbered 277, and correlation and path analysis was used with SPSS 10.0. The major findings were as follows: 1. It was shown that self-differentiation, over involvement and perceived criticism of premarital adult children were correlated with trait anxiety. And, among demographic variables, education, income, and marital status of the subjects' parents were related with trait anxiety. 2. Parent-child differentiation, over involvement, perceived criticism, and sex had direct effects on premarital adult child's self-differentiation. The variables that had direct effects on trait anxiety, which was the final dependent variable were self-differentiation, parent-child differentiation, perceived criticism, and income while over involvement an indirect effect on it.

• 지역사회간호학회지
• /
• 제18권4호
• /
• pp.673-680
• /
• 2007

Purpose: The purpose of this study was to investigate the relation between self differentiation and school adjustment among adolescents. Methods: The subjects were 356 adolescents. The following research questions were established. The adolescents' self differentiation level was measured based on Bowen's family system theory modified for Korean adolescents(Je, 1989) and their school adjustment level was measured with developed for Korean adolescents(Park, 1998). Collected data were analyzed by Mean${\pm}$SD, t-test, ANOVA and Schefff and Pearson correlation coefficient (SPSS 12.0). Results: 1. The mean score for the level of self differentiation was $99.99{\pm}11.58$ and school adjustment was $63.52{\pm}8.80$. 2. The self differentiation level was different according to father's education and monthly income. The school adjustment level was different according to glade, sex father's education and father's occupation. 3. Self differentiation had a significant positive correlation with school adjustment. Conclusion: The self differentiation level was confirmed to have an influence on school adjustment. We need to develop a program that can enhance adolescents' self differentiation.

• 한국가정과학회지
• /
• 제3권2호
• /
• pp.13-26
• /
• 2000

The purpose of present study were to find the general trends of mate selecting factors and. marital satisfaction. concentrated on married women, to examine the difference among mate selection factors and marital satisfaction according to socio-demographic variables and Psychological variables and to analyze the effects of these variables influencing marital satisfaction. The subjects were 276 wives, living in Kwangju that having passed under S years after marriage without divorce experience. The major findings were as follows . 1. In mate selection, factor of high-degree was personality. view of value. personal relations, achievement, emotional mature. self-differentiation. degree of affection's expression. sense of humor, charms and condition of health Respondents'marital satisfaction score showed 91.75 and this score was higher than median score(62.5) 2. The external factor of mate selection showed significant difference according to degree of education. career. order. and sex-role attitude. The internal factor of mate selection showed significant difference according to degree of education, career, order, self-differentiation, self-esteem, and sex-role attitude 3. As correlating mate selectional factors to marital satisfaction, the significance appears in the mate's personality. view of value, emotional mature. personal relations. self-differentiation, condition of health. achievement. charm, sense of humor and degree of affection's expression. 4. Married women's marital satisfaction was influenced by self-esteem, personality and child's number that were explained about 38% by these variables. In conclusion, to happy marital life must be loved her own self. and above all considered internal factors like personality than external factors in mate selection.

• 환경생물
• /
• 제39권2호
• /
• pp.160-168
• /
• 2021

Doublesex and mab-3 related transcription factor(dmrt) play crucial roles in sex determination and sex differentiation in vertebrates and invertebrates. Although dmrt genes have been identified in vertebrates, little is known about aquatic invertebrates. In this study, two dmrt genes, namely, Dc_dmrt93B and Dc_dmrt99B, were identified from brackish water flea, Diaphanosoma celebensis. Transcriptional changes were observed in the dmrt genes when the flea was exposed to bisphenol(BP), an endocrine disruptor. Sequence and phylogenetic analyses showed that both dmrt genes contained two conserved domains, namely, DM and DMA, closely clustered with those of Daphnia spp. Additionally, a significant increase in the Dc_dmrt99B mRNA expression level was observed upon exposure to intermediate concentrations of BP (bisphenol A>bisphenol S=bisphenol F, p<0.05), while the expression of Dc_dmrt93B mRNA was slightly modulated. These findings imply that the two dmrt genes may be involved in sex differentiation of D. celebensis. Furthermore, it was found that the ability of BP to modulate dmrt genes could affect development and reproduction. This study provides a basis for understanding the function of the dmrt genes and the molecular mode of action of BP in small crustaceans.

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
• /
• pp.224.2-224
• /
• 1995

No Abstract, See Full Text