• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.63-65
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• 2002

Identification of spermatogonial stem cell-specific surface molecules is important in understanding the molecular mechanisms underlying the maintenance and differentiation of these cells. We have found that spermatogonia from busulfan treated mice expressed an autoantigen that distinguishes between undifferentiated and differentiated spermatogonia. Four to six weeks after busulfan treatment, germ cells located in the basal compartment of seminiferous epithelium show isotype-specific IgG deposits that form due to autoimmunity. Before busulfan treatment, the level of testicular IgG was very low but IgG levels began to increase after week 4 and peaked at week 6. When cells from the busulfan treated testis were analyzed using laser scanning cytomeoy (LSC), the frequency of cells positive for IgG deposits, 6-integrin, and 1-integrin were 16.5${\pm}$3.8%, 11.8${\pm}$2.6%, and 9.0${\pm}$ 1.4%, respectively. Immunofluorescent staining suggested that most, if not all of the cells with IgG-deposits isolated from a laminin-coated dish, were also positive for a spermatogonial stem cell marker \ulcorner6-integrins as well as for a germ cell-specific marker TRA 98. We determined serum and intratesticular IgG levels and the soundness of seminiferous tubule basement membrane from busulfan treated mice using electron microscopy, in order to study the mechanism responsible for IgG deposits in spermatogonia. We found that the basement membranes of seminiferous tubules from busulfan treated mice were severely impaired when compared to those of normal adult, neonates and w/wv mice. Furthermore, new blood cells were observed in the surface of the damaged basement membrane along the seminiferous tubules. These results suggest that the IgG in spermatogonial stem cells accumulates from circulating blood through the impaired basement membranes induced by busulfan treatment. Taken together, our study suggests that IgG can be used as a new marker for undifferentiated spermatogonia cells.

• 식물병연구
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• 제16권1호
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• pp.1-9
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• 2010

Among the plant specimens requested from agricultural actual places of farmers, Agency of agricultural extension services and so forth for the diagnosis of plant virus diseases in 2009, the rate of crop types was 87.5% for vegetables, 4.0% for upland crops and 3.5% for orchids. In vegetables, the crops damaged severely by viral diseases were red pepper and tomato by the infection rate of 51.6% and 26.5%, orderly. Virus species occurring vegetables were 19 and the economically important viruses were Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV), Tomato yellow leaf curl virus (TYLCV), Pepper mild mottle virus (PMMoV) with the infection rate of 33.2%, 16.9%, 16.1% and 7.4%, respectively. Rice stripe virus (RSV) occurred at the whole areas of west coast in Korea in 2009, and its incidence was 14.2% mainly on the susceptible cultivars and yield loss was estimated up to 50%. TYLCV was spread at 34 areas of Si and/or Gun, 22 areas in 2009 and 12 in 2008. Distribution of TSWV was expanded newly in 6 areas of Si and/or Gun including Gangryung, Gangwondo in 2009, and its occurrence areas were 23 Si and/or Gun after first incidence at Anyang area in 2004. Tomato bushy stunt virus (TBSV) was incited newly at Gimcheon area in 2009 with the infection rate of 65.2%, and its soil transmission rate was 55.0% in average.

• 한국임상수의학회지
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• 제11권1호
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• pp.369-376
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• 1994

The purpose of this report Is to discribe the radiological findings of traumatic elbow luxation and subluxation of carpal joint which were caused by being hit by a wild animal and forced hyperextension of carpus in two hunting dogs. In case 1, carniocaudal and mediolateral radiographic examination of the elbow joint revealed that the radius that the radius and ulna were completely luxated laterally. The lateral projection showed the humeral condyles overlying the radial head with an absent joint space, but displacement is apparent on the craniocaudal projection. Incidence of collateral ligament rupture in conjunction with elbow luxation was not recognized on physical examination of the limb after closed reduction. Radiographic examination of the elbow made 5 months after closed reduction revealed only mild degenerative joint disease. Radiographic examination of the carpus in case 2 demonstrated palmar subluxation of most carpal joints including antebrachiocarpal, mediocarpal carpometacarpal joints, and some intercarpal joints, and avulsion fracture of the palmar surface of the accessory carpal bone. However, the mediocarpal and carpometacarpal joints were most severely affected by tensile forces of hyperextension, and it was concluded that the ligaments of the middle ca,pal joints and carpometacarpal joints as well as the palmar fibrocartilage were ruptured based on clinical and radiographic findings. In elbow luxation and subluxation of carpal Joints of the dog, differentiation of the joint or joints involved, and ligamentous structures damaged is important in determining whether surgery Is necessary and which procedure is most appropriate. And the radiographic findings of these conditions would be of help to practitioners.

• 한국수의병리학회지
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• 제3권1호
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• pp.35-44
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• 1999

The morphologic changes of small intestinal epithelium in pigs diagnosed as porcine epidemic diarrhea(PED} by virus isolation and immunohistochemistry were studied through light microscope and transmissible electron microscope. On semi-thin section, the histologic findings showed severe villous atrophy and fusion with hyperplasia of cuboidal epithelium in the villi, inflammatory cell infiltration in lamina propria, and increased mitotic figures in the crypt. The structural changes were mostly restricted to the cytoplasm of affected absorptive epithelium of villi. 3 types of epithelial changes were found; degenerated virus-affected cells, undifferentiated cuboidal cells, and normal columnar cells. On electron microscopy, round to spherical viral particles of 50∼l00nm in diameter were found within the dilated vesicles and endoplasmic reticulums of degenerated cells, which had decreased their cytoplasmic electron density due to dilated and missing organelles(e.g. mitochondria, ERs, etc.). Microvilli were shortened and sparse, leaving denuded terminal web of the villous epithelial cells. Fat globules were often found within slightly degenerated enterocytes. On the tip of villi, severely damaged cells were exfoliated and replaced by undifferentiated cuboidal cells We found distinct ultrastructural changes in the jejunal epithelium confirming PED virus infection is involved in malabsorptive diarrhea.

• 대한수의학회지
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• 제34권4호
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• pp.665-678
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• 1994

This study was undertaken to investigate histological changes according to the radiosensitivity in the spermatogenic cells in Korean native pheasants. During spermatogenetic period, testes wete collected from male adult Korean native pheasant and they were used as experimental and control birds. The experimental group was divided into a single-dose whole body irradiation group(400, 600, 800 and 1000 rads) and a split-dose whole body irradiation groups(400/2, 600/2, 800/2 and 1000/2 rads). A Henseky's $^{60}Co$ ${\gamma}$-radiotherapy machine was used for this experiment and the dose rate of $^{60}Co$ ${\gamma}$-ray was 104 rads/min. The experimental birds were sacrificed at 24 and 72 hrs after irradiation and the control pheasants were sacrificed at the same time. General histological changes of seminiferous epithelial cells were observed by hematoxylin-eosin stain with light microscope. The results obtained are summarized as follows ; 1. In the single-dose and the split-dose irradiation groups, the average diameter of the seminiferous tubule was decreased compared with control group. 2. Seminiferous epithelial cells were more severely damaged after 72 hrs than after 24 hrs of single-dose irradiation of 400, 600 and 800 rads but the difference of cell injury was almost not observable with the elapsed time in the group of the single-dose irradiation of 1000 rads. 3. The damage of spermatogenic cells were more severe after 24 hrs than after 72 hrs of the split-dose irradiation of 400 rads but the split-dose irradiation of 600, 800 and 1000 rads were more severe after 72 hrs than after 24 hrs.

• 환경생물
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• 제38권3호
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• pp.486-495
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• 2020

토마토와 박과류채소의 유기농산물을 생산하기 위하여 무농약으로 재배하는 시설하우스에서 최근 문제되는 병해를 조사한 결과 토마토 잎곰팡이병과 토마토반점위조바이러스병, 토마토퇴록바이러스병, 박과류 쥬키니황화모자이크바이러스병, 박과류 흰가루병 등의 피해가 심한 것으로 조사되었다. 농약을 사용하여 일반재배를 하는 시설하우스에 비해 무농약재배 하우스에서 재배되는 작물에서 병 발생 시기가 더 빠르고 병 발생도 심한 것으로 나타났으며, 총채벌레와 진딧물 등에 의해 매개되는 바이러스병 피해가 심한 것으로 조사되었다. 따라서 우수한 유기농산물을 생산하기 위해서는 병해충방제를 효율적으로 하는 것이 중요하며, 병 발생 피해를 최소화기 위해서는 포장위생과 물리적 차단, 유기농업자재나 미생물제 등을 종합적으로 활용하여 예방적으로 방제하는 것이 가장 중요하다.

• Earthquakes and Structures
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• 제1권4호
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• pp.371-390
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• 2010

Historical buildings in seismically active regions are severely damaged by earthquakes, since they certainly were not designed by the original builders to withstand seismic effects. In particular the reports after major ground motions indicate that earthquake-induced pounding between buildings may lead to substantial damage or even collapse of colliding structures. The research on structural pounding during earthquakes has been recently much advanced, although most of the studies are conducted on simplified single degree of freedom systems. In this paper a detailed pounding-involved response analysis of three adjacent structures is performed, concerning the main bodies of the "Quinto Orazio Flacco" school. The construction includes a main masonry building, with an M-shaped plan, and a reinforced concrete building, separated from the masonry one and realized along its free perimeter. By the analysis of the capacity curves obtained by suitable pushover procedures performed separately for each building, it emerges that masonry and reinforced concrete buildings are vulnerable to earthquake-induced structural pounding in the longitudinal direction. In particular, due to the geometric configuration of the school, a special case of impact between the reinforced concrete structure and two parts of the masonry building occurs. In order to evaluate the pounding-involved response of three adjacent structures, in this paper a numerical procedure is proposed, programmed using MATLAB software. Both a non-linear viscoelastic model to simulate impact and an elastic-perfectly plastic approximation of the storey shear force-drift relation are assumed, differently from many commercial softwares which admit just one non-linearity.

• 한국응용곤충학회지
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• 제29권2호
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• pp.104-112
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• 1990

남부지방에서 뽕나무깍지벌레의 기주식물, 생활사 및 천적에 관하여 조사, 연구한 결과는 다음과 같다. 뽕나무깍지별레의 기주식물은 總 22科 74種이 조사되었으며 특히 Prunus層의 核果類에 피해가 많았다. 또한 本蟲은 연 3세대를 경과하며 유충 발생시기는 1세대가 5월 중순~6월 하순, 2세대 7월 중순~8월 중순, 3세대 9월 초순~10월 하순이었으며 최성기는 각각 6월 상순, 7월 하순, 9월 중순이었다. 난, 유충 및 雌成蟲의 개체군 밀도에 있어서는 최초 발생일로부터 각각 10일, 25일, 7일경에 가장 높았으며 뽕나무깍지벌레 천적은 Aphytis diaspidis, Archenomus orientalis, Apterencyrtus morophagus 등 기생봉 3種과 홍점박이무당벌레, 애홍점박이무당벌레, 무당벌레1種 등 3種의 포식충이 조사되었다. 뽕나무깍지벌레와 고약병균 감염은 신초신장과 및점한 관계가 있고 가해받지 않은 가지에 비해서 신초신장이 1/2, 1/4정도 밖에 이루어 지지 않았다.

• 한국응용곤충학회지
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• 제55권4호
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• pp.431-437
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• 2016

시설딸기에 발생하는 해충 종류와 피해를 구명하기 위하여 2013년부터 2016년까지 제주지역의 농가포장에서 조사한 결과 8목 31종이 발생되고 있었다. 그중에서 발생과 피해가 큰 해충은 점박이응애(Tetranychus urticae), 꽃노랑총채벌레(Frankliniella occidentalis), 목화진딧물(Aphis gossypii) 3종이었다. 그 외에 딸기가루이와 괴테애매미충은 일부 조사포장에서 피해가 나타났으나 발생과 피해가 점차 증가하고 있었다. 나방류 중에서는 담배거세미나방(Spodoptera litura)의 발생빈도와 피해가 가장 컸으며, 왕담배나방(Helicoverpa armigera)은 조사기간 중 발생빈도는 적었으나 피해는 심했다. 조사 해충 중 황색끈끈이트랩으로 발생밀도를 예찰할 수 있는 꽃노랑총채벌레와 목화진딧물 유시충, 딸기가루이, 괴테애매미충에 대한 시기별 발생특성에 대하여 고찰하였다.

• 대한치과보철학회지
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• 제24권1호
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• pp.125-138
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• 1986

It is considered that etching solution or material itself of phosphoric ester cement will induce not a little pulpal irritation, if applied directly onto unsealed dentinal tubules. This study was designed to confirm above consideration by comparing two different conditions between $Ca(OH)_2$-based and non-$Ca(OH)_2$-based group. Posterior teeth of 15 male dogs were selected for this experiment. One experimental group was filled with cement after $Ca(OH)_2$-basing and enamel-etching, the other experimental group after enamel etching without $Ca(OH)_2$-basing. And both of two experimental groups were observed at 2 hours, 15 hours, 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks after filling. The findings reached to the following conclusions histologically. 1. In both groups, the damaged odontoblasts were atrophied and eventually disappeared. 2. In non-based group at early stage, odontoblasts were severely atrophied and defective areas were appeared between odontoblast cell layers. However, in based group, the odontoblasts were arranged slight irregularly. 3. In non-based group, a small number of undifferentiated cells below the odontoblast cell layers started to appear at 1 week after filling. However, in based group, the undifferentiated cells were appeared at 15 hours after filling. 4. In non-based group, formation of reparative dentin was not begun until late stage of experiment. However, in based group, formation of reparative dentin matrix was begun at 2 weeks after filling and very thickened reparative dentin was formed at 6 weeks after filling. 5. In odontoblast cell layers of both groups, dilated capillaries were observed. 6. Argyrophilic fibers were reticularly condensed in zone of Weil, and they were connected to the pulp tissue and dentin.