• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.209-213
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• 1998

The sesaminol glucosides in 80% EtOH extract from sesame seeds were separated by high performance liquid chromatography (HPLC). A HPLC system using a Develosil ODS-5 column and gradient elution system from 30% to 80% methanol was selected for separation and quantitative determination of sesaminol triglucoside, sesaminol diglucoside, and sesaminol monoglucoside. Quantitative analyses for these sesaminol glucosides, sesaminol triglucoside, sesaminol diglucoside, and sesaminol monoglucoside were determined on the basis of standard curve of sesaminol glucosides. Sesaminol triglucoside, sesaminol diglucoside and sesaminol monoglucoside contents of the seed of one Korean sesame cultivar, Danbaekggae, were 56.4 mg/100g, 9.6 mg/100g, and 7.5 mg/100g, respectively. The most abundant aglycon of lignan glucosides in sesame seed was sesaminol triglucoside

• Journal of Crop Science and Biotechnology
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• v.10 no.3
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• pp.185-192
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• 2007

Near-infrared spectroscopy(NIRS) was used to develop a rapid and efficient method to determine lignan glucosides in intact seeds of sesame(Sesamum indicum L.) germplasm accessions in Korea. A total of 93 samples(about 2 g of intact seeds) were scanned in the reflectance mode of a scanning monochromator, and the reference values for lignan glucosides contents were measured by high performance liquid chromatography. Calibration equations for sesaminol triglucoside, sesaminol($1{\rightarrow}2$) diglucoside, sesamolinol diglucoside, sesaminol($1{\rightarrow}6$) diglucoside, and total amount of lignan glucosides were developed using modified partial least square regression with internal cross validation(n=63), which exhibited lower SECV(standard errors of cross-validation), higher $R^2$(coefficient of determination in calibration), and higher 1-VR(ratio of unexplained variance divided by variance) values. Prediction of an external validation set(n=30) showed a significant correlation between reference values and NIRS estimated values based on the SEP(standard error of prediction), $r^2$(coefficient of determination in prediction), and the ratio of standard deviation(SD) of reference data to SEP, as factors used to evaluate the accuracy of equations. The models for each glucoside content had relatively higher values of SD/SEP(C) and $r^2$(more than 2.0 and 0.80, respectively), thereby characterizing those equations as having good quantitative information, while those of sesaminol($1{\rightarrow}2$) diglucoside showing a minor quantity had the lowest SD/SEP(C) and $r^2$ values(1.7 and 0.74, respectively), indicating a poor correlation between reference values and NIRS estimated values. The results indicated that NIRS could be used to rapidly determine lignan glucosides content in sesame seeds in the breeding programs for high quality sesame varieties.

• Journal of the East Asian Society of Dietary Life
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• v.14 no.4
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• pp.348-354
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• 2004

중국, 수단, 인도 및 파키스탄에서 생산된 참깨(각 10종)의 조지방 함량, 지방산 조성, 세사민, 세사몰린 및 세사미놀 배당체 함량을 측정하여 품질 특성을 평가하였다. 참깨의 조지방 함량은 파키스탄산 42.47 %, 중국산 38.95%, 인도산 34.96% 및 수단산 33.93%로 파키스탄산이 가장 높았으나 지역간에는 유의적인 차이가 나타나지 않았다. 세사민 함량은 중국산 634.75 mg/100 g seed, 수단산 630.66 mg/100 g seed, 인도산 381.55 mg/100 g seed 및 파키스탄산 401.00 mg/100 g seed으로 중국산이 유의적으로 높게 나타났다. 세사몰린 함량은 중국산 218.99 mg/100 g seed, 수단산 234.49 mg/100 g seed, 인도산 (185.64 mg/100 g seed) 및 파키스탄산 (127.04 mg/100 g seed)으로 지역간에 유의적인 차이가 있는 것으로 나타났다. 세사미놀 배당체 함량은 중국산 321.40 mg/100 g seed, 수단산 218.99 mg/100 g seed, 인도산 505.04 mg/100 g seed 및 파키스탄산 342.87 mg/100 g seed로 인도산이 가장 높은 함량이었다. 참깨 핵산 추출물의 전자공여능을 측정한 결과 인도산의 전자공여능이 유의적으로 높았고 파키스탄산 및 중국산순 이었다. 이상의 결과 지역별 참깨 품질 특성에는 유의적인 차이가 있었고 특히 최근에 기능성 성분으로 관심이 집중되고 있는 세사민과 세사몰린 및 세사미놀 배당체는 지역에 따라 현저한 차이가 있는 것으로 나타났다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.443-447
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• 2002

This study was carried out to determine the differences in the growth, grain yield, and seed quality of sesame plant according to seeding date between P,E. vinyl-house and outdoor cultures. Reproductive growth period in vinyl-house culture was shorter than in outdoor culture. Stem length and capsule setting length of sesame were much longer in vinyl-house culture than in outdoor culture. Also, number of capsules per plant and 1,000 grain weight in vinyl-house culture were higher, specially the grain yield was approximately 57% more than in outdoor culture. In vinyl house culture, sesame plants sown on June 8 had longer capsule setting length, more capsules per plant, higher 1,000 grain weight, and higher percent ripened grain at the upper part of the capsule settings than those sown in May 9. They also had higher 1,000 grain weight at the middle and lower part of the capsule settings compared to May 9 seeding. However, no difference in grain yield of in seeding dates was observed. In outdoor culture, sesame plants, which was sown on May 9, had more effective branch number and capsule number and plant compared to those sown on June 8. Though sesame plants sown on May 9 had lower percent ripened grain at the upper and middle part of the capsule settings and lower 1,000 grain weight, the seed yield was similar to those sown on June 8. No difference in chromaticity value $L^*$ of sesame seeds between two culture conditions was observed. The $a^*$ value was higher in vinyl-house culture than outdoor culture while $b^*$ value was higher in outdoor culture. Sesaminol triglucoside content of sesame seeds was higher in vinyl-house culture than in outdoor culture. On the other hand, the content of sesamin and sesamolin from sesame seeds in vinyl-house culture were lower than in outdoor culture.