• 한국식품과학회지
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• 제28권2호
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• pp.226-231
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• 1996

복숭아 펄프를 serum-pulp방법에 따라 serum과 불용성 펄프로 분리하고 serum만을 농축하면서 초기에 발생하는 증기를 회수하여 방향성분 획분으로 사용하였다. 농축 복숭아 펄프는 농축 serum, 불용성 펄프와 회수한 방향성분을 흔합제조하여 전체적으로 2배 농축하였다. 농축 복숭아 펄프의 향기성분은 DHC법으로 추출하여 GC와 GC/MS로 분석하고 관능검사한 결과 10%회수된 방향성분 획분을 첨가하는 것이 우수한 향기성분을 유지하는 고품질의 농축 펄프를 생산하는데 적합한 조건으로 나타났다.

• 한국식품영양학회지
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• 제6권4호
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• pp.281-286
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• 1993

Strawberry pulp was separated into serum and Insoluble pulp by centrifugation. The serum was concentrated in vacuo at 55~58$^{\circ}C$ and 10% aroma recovered. Two folds concentrated strawberry pulp was prepared by mixing concentrated serum, aroma fraction and Insoluble pulp. To develop aroma-rich jam, the strawberry jam was prepared by filling mixture of concentrated pulp, sucrose, citric acid and pectin into retortable pouches and by heating them after heat-seating. The color and flavor quality of the jam prepared by new method were better than the jam prepared by conventional method, as Indicated by GC, GC/MS data, surface color and anthocyanin contents. These results were confirmed by the sensory evaluations. Flavor off-flavor, color intensity and overall acceptance were superior to the conventional method.

• 한국식품과학회지
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• 제27권6호
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• pp.921-927
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• 1995

복숭아 펄프를 11,000 rpm에서 10분간 원심분리하여 serum과 불용성 펄프를 분리하였고, serum을 $50{\sim}55^{\circ}C$, $30{\sim}50\;mmHg$에서 농축하면서 초기에 발생하는 증기를 회수하여 방향성분 획분으로 하였다. 방향성분 획분은 serum의 양에 대하여 $0{\sim}5%$(AR-1), $5{\sim}10%$(AR-2), $10{\sim}15%$(AR-3), $15{\sim}20%$(AR-4)로 하였다. 방향성분 획분의 휘발성 향기성분은 DHC법에 의하여 포집하였고, 포집한 향기성분을 동정하기 위하여 GC/MS를 사용하여 분석하였다. 복숭아 펄프의 serum 분리율은 70.5%였으며, 대부분의 향기성분들은 serum에서 더 높게 나타났다. Ethylene, benzaldehyde, l-limonene, ${\gamma}-dodecalactone$ 등의 31종의 향기성분이 확인되었으며, 향기성분의 상대적인 총량은 AR-1에서 AR-4로 갈수록 감소하였으며 피크의 면적 및 갯수도 점차적으로 줄어들었다.

• Journal of Biosystems Engineering
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• 제38권2호
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• pp.113-120
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• 2013

Purpose: Stem cells provide new opportunities in the regenerative medicine for human or animal tissue regeneration. In this study, we report an efficient method for the modulating behaviors of electro-active stem cells by micro-electric current stimulation (mES) without using chemical agents, such as serum or induction chemicals. Methods: Dental pulp stem cells (DPSCs) were cultured on the tissue culture dish in the mES system. To find a suitable mES condition to promote the DPSC functions, the response surface analysis was used. Results: We found that a working micro-current of 38 ${\mu}A$ showed higher DPSC proliferation compared with other working conditions. The mES altered the expressions of intracellular and extracellular proteins compared to those in unstimulated cells. The mES with 38 ${\mu}A$ significantly increased osteogenesis of DPSCs compared with ones without mES. Conclusions: Our findings indicate that mES may induce DPSC proliferation and differentiation, resulting in applying to DPSCs-based human or animal tissue regeneration.

• Restorative Dentistry and Endodontics
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• 제24권1호
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• pp.1-12
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• 1999

Bacterial infection of the pulp results in the development of a periapical lesion with the concomitant resorption of periapical bone. The cytokines are believed to play an important role in this matter. The purpose of this study was to find the relationship among the presence of black pigmented bacteria, the levels of cytokines(TNF-${\alpha}$, -${\beta}$, IL-$1{\beta}$, and TGF-${\beta}1$), and the amount of bone resorption in periapical and pulpal diseases. For the purpose, the patients were grouped into chronic apical pathosis, acute apical pathosis, acute pulpitis, and a healthy control group. Root canal samples were taken from periapical tissue exudates during routine endodontic treatment, and the venous blood was taken from each patients. The samples were processed to measure local and systemic levels of the cytokines using enzyme linked immunosorbent assay(ELISA). Bacterial content of Porphyromonas endodontalis, Porphyromonas gingivalis, and Prevotella nigrescens were measured by indirect immunofluorescence method and the size of the periapical lesions were measured from the radiographs. The following results were obtained: 1. The levels of bone resorptive cytokines(TNF-${\alpha}$, TNF-${\beta}$, and IL-$1{\beta}$) in exudates from acute and chronic apical pathoses were significantly higher than those from acute pulpitis and the normal pulps(p<0.05). 2. IL-$1{\beta}$ were the highest among the bone resorptive cytokines in apical pathoses. However, no statistical difference between acute and chronic lesions were found(p>0.05). 3. The levels of TGF-${\beta}1$ in exudates from acute pulpitis and chronic apical pathoses were significantly higher than those from acute apical pathoses and the normal pulps(p<0.05). However, there were no significant correlations among the levels of bone resorptive cytokines. 4. The levels of TNF-${\beta}$ in serum were significantly higher than those from the exudates while serum TGF-${\beta}1$ concentrations were significantly lower(p<0.05). 5. Exudates from the canals in which the P. nigrescens were detected showed significantly higher levels of IL-$1{\beta}$ than those from the canals without the microorganism(p<0.05). 6. There were no significant correlations among the levels of the cytokines, the amount of bone destruction, and the presence of acute and chronic symptoms(p>0.05).