• Journal of Photoscience
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• 제11권1호
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• pp.29-33
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• 2004

The mechanism of interaction of cyanocobalamin (CB) with bovine serum albumin (BSA) has been investigated by spectrofluorometric and circular dichroism methods. Association constant for the CB-BSA system showed that the interaction is non-covalent in nature. Binding studies in the presence of an hydrophobic probe, 8-anilino-l-naphthalene sulphonic acid, sodium salt (ANS) showed that there is hydrophobic interaction between CB and ANS and they do not share common sites in BSA. Stern-Volmer analysis of fluorescence quenching data showed that the fraction of fluorophore (protein) accessible to the quencher (CB) was close to unity indicating thereby that both tryptophan residues of BSA are involved in drug-protein interaction. The rate constant for quenching, greater than $10^{10}$ $M^{-1}$ $s^{-1}$, indicated that the drug binding site is in close proximity to tryptophan residue of BSA. Thermodynamic parameters obtained from data at different temperatures showed that the binding of CB to BSA involves hydrophobic bonds predominantly. Significant increase in concentration of free drug was observed for CB in presence of paracetamol. Circular dichroism studies revealed the change in helicity of BSA due to binding of CB to BSA.

• Journal of Nutrition and Health
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• 제42권8호
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• pp.759-769
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• 2009

This study investigated the effects of the nutrition education and exercise program on body composition, dietary intakes and physical fitness in obese women. The subjects were 44 obese women (BMI${\geq}$ 25 kg/$m^2$) who had been participating in nutrition education (1 time/week) and exercise program (3 time/week) for 12 weeks. Nutrition education was focused on low energy, high protein and low carbohydrate diet to reduce the body weight and improve the diet quality. To evaluate the effectiveness of the program, daily nutrient intakes were assessed by 24-hour recall method. Body composition, blood lipid profiles and physical fitness test were assessed before and after the intervention. After the intervention, body weight, BMI, percent body fat, soft lean mass and waist/hip ratio were significantly decreased (p < 0.001). Fasting blood sucrose, total cholesterol, LDL-cholesterol and atherogenic index (AI) were significantly decreased (p < 0.001), while HDLcholesterol was significantly increased (p < 0.001). Energy adjusted protein, fiber, vitamin A, vitamin $B_2$, vitamin $B_6$, vitamin C, vitamin E, niacin and folate were significantly increased. After the intervention, the muscle endurance, muscle strength, agility, balance were significantly increased. The changes in obesity indices (body weight, BMI, percent body fat, waist-hip ratio) were correlated with the changes of the nutrient intakes, serum lipid profiles, physical fitness. These results show that nutrition education and exercise program was effective not only for weight reduction but also for the improvement of physical strength and cardiovascular disease risk factors in obese women.

• 대한수의학회지
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• 제15권2호
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• pp.327-332
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• 1975

Parasitological and biochemical studies for 82 heads (12.2%) showing sterility syndrom out of 672 heads of Korean native cattle were undertaken by analysis of blood values for their contents and examination of their vaginal swabs for Tritrichomonas foetus 1. Tritrichomonas infection of Korean cattle revealed 7 positive cases (8.54%) out of 82 infertile heads. 2. Contents of their serum protean, carotenoid, vitamin A and phosphorus were determined in summer and winter season and the following results were obtained. a) In summer season, average blood value of 30 cattle showing sterility syndrom were determined as protein 7.52g/100ml, carotenoid 165.1g/100ml, vitamin A 173.9 IU/100ml and phosphorus 7.48mg/100ml, respectively. b) In winter. season, average blood value of 52 cattle showing sterility syndrom were determined as protein 7.76g/100ml, carotenoid 413.3g/100ml, vitamin A 174,3 IU/100ml and phosphorus 7.54mg/100ml, respectively.

• 대한지역사회영양학회지
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• 제8권5호
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• pp.667-674
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• 2003

The paper examines the effect of physical exercise on blood lipid with young male students. Our analysis is based on the body measurement, food intake and blood lipid component for 70 male students of Kongju National University who either major in physical exercise (group A) or do not major in physical exercise (group B). The average weight of group A is 71.97 $\pm$ 10.79 kg while that of group B is 67.57 $\pm$ 8.66 kg. The former is significantly higher (p<0.05) than the latter. The SBP of group A (128.20 $\pm$ 11.40 mmHg) is significantly (p<0.001) higher than that of group B (136.27 $\pm$ 13.18 mmHg). In terms of the intake of total kcal, group A marks significantly higher than group B; group A goes with 2190.26 $\pm$ 581.96 kcal and group B goes with 2392.94 $\pm$ 769.03 kcal. The comparative analysis of the three nutrient intake shows that the intake proportions for group A are carbohydrate 55.08 $\pm$ 6.03%, protein 15.58 $\pm$ 2.75%, and lipid 29.34 $\pm$ 5.16% while the intake proportions for group B are carbohydrate 57.29 $\pm$ 8.09%, protein 15.62 $\pm$ 3.26%, and lipid 27.10 $\pm$ 6.90%. For group A, the vitamin B$_1$ intake amounts to 1.50 $\pm$ 0.5 mg which fulfills 100% of RDA, and the niacin intake amounts to 16.57 $\pm$ 5.54 mg N.E which is less than RDA. In contrast, both intakes for group B are more than the RDA. In the case with minerals except calcium, both group A and B mark more intake than RDA. Group A's calcium intake is 517.12 $\pm$ 200.63 mg and group B's is 409.56 mg. The total cholesterol intake for group A is 447.00 $\pm$ 245.08 mg which is significantly (p<0.05) higher than for group B with 352.35 $\pm$ 200.25 mg. The total cholesterol in the lipid of serum is 151.30 $\pm$ 12.92 mg/dl for group A and 182.30 mg/dl for group B, where the dominance of group B over group A is true at the level of significance 0.01. In the case with triglyceride, group A is 107.57 $\pm$ 31.60 mg/dl and group B is 108.07 $\pm$ 33.93 mg/dl so that the group difference is non-significant. The HDL-cholesterol for group A is 54.67 $\pm$ 6.49 mg/dl which is significantly higher (p<0.001) than for group B with 36.13 $\pm$ 4.64 mg/dl. We conclude that regular exercise may have an effect on blood lipid metabolism among young males.

• Asian-Australasian Journal of Animal Sciences
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• 제16권4호
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• pp.519-528
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• 2003

A survey was conducted in certain parts of Haryana to record the prevalence of micro mineral deficiency in buffaloes. The prevalence of soil Cu, Co, Zn and Fe deficiency was 55.26%, 6.9%, 59.12% and 7.89% respectively. While that of fodder Cu, Co, Zn and Fe was 60.64%, 6.7%, 61.22% and 11.37% respectively. The overall prevalence of serum Cu, Co, Zn and Fe deficiency in Haryana was 59.2%, 19.1%, 59.2% and 19.9% respectively. The correlation co-efficient of Cu, Co, Zn and Fe in soil, fodder and serum was significant in most of the cases the values were above 0.8. Blood examination revealed significant decrease in haemoglobin and TEC level. However, no variation in level of TLC were observed in mineral deficient buffaloes. In micro mineral deficiency, thyroid hormone (T3 and T4) levels were decreased in buffaloes. Marginally lower concentration of vitamin A and E were observed in mineral deficient buffaloes in Haryana. The highest deficiency of micro minerals was 61.76% in copper at Ambala followed by 65.86% in zinc at Rhotak. For therapeutic studies a mineral mixture was prepared according to defiency obtained and fed to three groups of animals. Observation was recorded on 0, 15, 30, 45 and 60 days. Group A consist of normal healthy animals and group B mineral deficient animal untreated and group C mineral deficient animal, treated with prepared mineral mixture. 25 gram of mineral mixture was fed daily along with normal ration. There was increase in body weight, milk yield, haemoglobin concentration and total erythrocyte level in group C animals when compared to group B animals. The milk yield in group C animals increased to 6.970${\pm}$0.41 after 60th day of supplement in comparison to 0 day where it was 5.910${\pm}$0.37, similarly the body wt. of group C animals increased from 129.42${\pm}$01.13 (at 0 day) to 159.31${\pm}$03.61 at 60th day of treatment.

• 한국식품과학회지
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• 제35권6호
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• pp.1204-1208
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• 2003

항암제인 cyclophosphamide를 흰쥐에 투여하여 유발된 빈혈증상에 대하여 한국당귀, 중국당귀, 일당귀의 투여가 빈혈회복에 미치는 효능을 비교한 연구로서, Sprague-Dawley계 수컷 흰쥐에게 cyclophosphamide를 30mg/kg BW/day로 3회 복강 투여 후, 참당귀, 중국당귀와 일당귀 추출물을 1 g/kg BW/day로 14일 동안 경구 투여하였다. Cyclophosphamide 투여로 체중감소는 대표적인 전신증상으로 나타났으나 당귀추출물의 투여가 cyclophosphamide에 의한 체중감소 부작용을 저해함으로써 체중증가를 유도한 것으로 볼 수 있다. 참당귀 투여군은 적혈구 용적(hematocrit, Hct) 변화, 적,백혈구수 변화, 혈색소 농도 변화, 혈청 iron, transferrin 포화도 등의 항목에 대하여 유의성 있는 회복 효과를 나타내었고, 중국 및 일당귀 투여군은 백혈구수 감소에 대하여 유의성 있는 회복효과를 나타내었다. Cyclophosphamide 투여 후 비타민 $B_{12}$ 농도는 정상군에 비해 유의적으로 낮았고, 중국당귀, 참당귀, 일당귀 순으로 비타민 $B_{12}$ 농도가 유의적으로 증가하는 것을 관찰하였다. 이는 당귀가 비타민 $B_{12}$의 농도를 증가시키면서 혈액생산작용에 도움을 줄 것으로 여겨진다. 결론적으로 참당귀추출물 투여로 인해 혈액수치가 향상된 결과는 암 환자가 화학요법으로 인하여 생긴 빈혈치료에 도움이 될 것이라 생각된다.

• 대한지역사회영양학회지
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• 제5권2호
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• pp.289-296
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• 2000

The purpose of this study was to investigate the relationship and lipids in healthy adults on self-selected diets. These subjects consisted of 40 female college students residing in Chungnam. Anthropometric measure-ments, diet intake measurements, and blood collection were conducted. Serum concentrations of 5 microminerals(As, Cr, Mn, Se, Ni), lipids(triglyceride, total cholesterol, HDL-cholesterol), and glucose were measured by an ICP spectrometer and biochemical analyzer. The results were as follows. The mean age of the subjects was 22.34 years and the mean weight, height, and BMI were 52.89kg, 161.29cm and 20.34, respectively. The mean serum concentrations appeared to be 14.60ug/dl(As), 1.87ug/dl(Cr), 0.18ug/dl(Mn), 23.50ug/dl(Se), 0.21ug/dl(Ni), 60.73mg/dl(triglyceride), 138.49mg/dl(total cholesterol), 65.95mg/dl(HDL-cholesterol), 60.39mg/dl(LDL-cholesterol) and 88.82mg/dl(glucose). When analyzed by Pearson’s correlation coefficient, the serum concentration of Cr was negatively correlated with Ca and vitamin B12 intake(p〈0.05, p〈0.05) respectively, Mn was negatively correlated with Na intake(p〈0.05), Ni, however, was positively correlated with K intake(p〈0.05). The serum concentration of Se was positively correlated with LDL-cholesterol(p〈0.05), Ni, however, negatively correlated with total cholesterol, HDL-cholesterol, LDL-cholesterol, and glucose, respectively(p〈0.001, p〈0.01, p〈0.05). Further studies are needed to clarify the precise micromineral intakes, nutritional assessment of microminerals, and cause-effect relation of microminerals and serum lipids.

• 대한화장품학회지
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• 제27권1호
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• pp.17-27
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• 2001

A cell-based assay system for monitoring NF-$textsc{k}$B activity was developed to determine the influence of activated NF-$textsc{k}$B in human HaCaT cells. The pNF-$textsc{k}$B-SEAP-NPT plasmid that permits expression of the secreted alkaline phosphatase (SEAP) reported gene in response to the NF-$textsc{k}$B activity and contains neomycin phosphotransferase (NPT) gene for the geneticin resistance in host cells was constructed and transfected into human keratinocyte cell line HaCaT. Human HaCaT transfectant cells secreted the SEAP enzyme into the culture medium in a time-dependent manner until 72h. NF-$textsc{k}$B activities were measured in the SEAP reporter gene assay using a fluorescent detection method. The treatment of HaCaT cell transfectants with known antioxidants [e.g., N-acetyl-L-cysteine and vitamin C] showed inhibition of NF-$textsc{k}$B activity in a time-and concentration-dependent manner. The phorbol 12-myristate 13-acetate (PMA) known as a stimulator of NF-$textsc{k}$B expression demonstrated that it increased NF-$textsc{k}$B activity in a time- and concentration-dependent manner. This assay system could be used to determine the quantitative measurement of NF-$textsc{k}$B activity in the human skin and allow the screening of anti-inflammatory agents from various synthetic chemicals and natural products for dermatological purpose. Abbrevitions used: NF-$textsc{k}$B, nuclear factor kappa B; I-$textsc{k}$B, Inhibitory kappa B; SEAP, secreted alkaline phosphatase; NPT, neomycin phosphotransferease; PCR, polymerase chain reaction: dNTP, deoxynucleoside triphosphates; DMEM, dulbecco’s modified eagle medium; FBS, fetal bovine serum; PBs, phosphate-buffered saline; MUP, 4-methylumbellifery phosphate; NAC, N-acetyl-L-cysteine; DMSO, dimethyl sulfoxide; PMA, phorbol 12-myristate 13-acetate.

• Journal of Nutrition and Health
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• 제36권10호
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• pp.1013-1021
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• 2003

Anemia, the condition of the diminished concentration of hemoglobin per erythrocyte is common in patients with cancer and is a frequent complication of myelosuppressive chemotherapy. Cham-Dang-Gui (Angelicae Gigantis Radix) has been used in traditional Korean medicine to treat hematologic deficiencies. In this study, Cyclophosphamide (CYP), an alkylating agent that has a broad spectrum of anti-cancer activities, was intraperitoneally injected into the experimental animals to suppress the bone marrow thereafter, causing anemia. The hemopoietic effects of Cham-Dang-Gui were examined using anemic rat model. Rats were divided into five groups: CON (control group), ANS (CYP-injected + normal diet), AND (CYP-injected + normal diet + Cham-Dang-Gui), ALS (CYP-injected + low iron diet), and ALD (CYP-injected + low iron diet + Cham- Dang-Gui) groups. CYP (30 mg/kg) was intraperitoneally injected to rats for 3 days to induce anemic condition. Saline or Cham-Dang-Gui was administrated orally during the entire experimental period. CYP injection decreased body weight gain and food consumption in comparison with CON group. Oral administration of Cham-Dang-Gui extract with normal iron diet significantly prevented the lower body weight gain. The blood level of hemoglobin, iron status (serum iron, transferrin, ferritin and TIBC) and blood level of vitamin B-12 in Cham-Dang-Gui treated groups were significantly higher than those of Cham-Dang-Gui untreated groups regardless of amount of iron in the diet. Taken together, it could be concluded that the Cham-Dang-Gui extract could improve anemic condition induced by CYP injection by improving hematological value, iron status and vitamin B12 status in rats.

• Journal of Nutrition and Health
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• 제36권2호
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• pp.191-199
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• 2003

This study was conducted to evaluate nutritional status of Korean women of child-bearing age. A comparison was made between 113 non-pregnant and 200 pregnant women. Pregnant women of 24-28 weeks of gestation were recruited from prenatal clinic in Seoul. Nonpregnant women were included college students, nurses, office employers, and sales women. General characteristics, anthropometric measurements, dietary intakes, and hematological values were assessed. There was no difference in mean BMI for nonpregnant and pregnant (pre-gravid) women, which were 20.2 kg/m$^2$and 20.6 kg/m$^2$respectively. The pregnant women showed a tendency of better health-caring behavior, evidenced by stop smoking and drinking, and in taking nutrient supplements. The mean intakes of energy, vitamin A, vitamin B$_2$, calcium, and iron did not meet Korean RDA for adult women. In particular, calcium and iron intakes of both nonpregnant and pregnant women were under 60% of the Korean RDA. Judging by MDA score, dietary quality was better in pregnant than in nonpregnant women. Mean serum values of albumin, total cholesterol, LDL-cholesterol, HDL-cholesterol, Hb, IgA, IgG were in normal range. However, more than 13% of the nonpregnant subjects showed mild hypercholesterolemia (cholesterol > 200 mg/dl) and anemia (Hb < 12.0 g/dl) . The percentage of anemia in pregnant women were much higher; the subject with Hb < 11.0 g/dl were approximately 30%. The pregnant subjects showed significantly higher serum concentrations of triglycerides and cholesterol, and significantly lower concentrations of albumin, IgA, and IgG compared to nonpregnant women. Correlation analysis showed that Hb concentrations were correlated with the intakes of iron- and protein- containing foods such as meat and vegetables. This study strongly suggest that iron-deficient anemia is a major nutrition problem in Korean child-bearing women and this condition is correlated with dietary intakes.