• Macromolecular Research
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• 제13권1호
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• pp.39-44
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• 2005

Ion-exchange polymeric stationary phases presenting amino acid and amino groups were prepared by the surface grafting of glycidyl methacrylate onto a silica gel surface and subsequent amination. Three kinds of amino acids-L-arginine (Arg), D-lysine (Lys), and D-histine (His)-were used in this study. An ion-exchange polymeric stationary phase presenting ethylene diamine (EDA) was also prepared by surface graft polymerization. Separation of the model proteins bovine serum albumin (BSA), chick egg albumin (CEA), and hemoglobin (Hb) was performed using the amino acid- and amine-derived columns. In separating the CEA/BSA mixture, the resolution time of BSA was longer than that of CEA when using the EDA column, whereas the resolution time of BSA was shorter than that of CEA when using the Arg, Lys, and His columns. In the separation of the Hb/BSA mixture, the resolution time of BSA was longer than that of Hb in the EDA column, whereas the resolution time of BSA was shorter than that of Hb in the amino acid columns (D-Lys, L-Arg, and D-His).

• 한국수정란이식학회지
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• 제20권3호
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• pp.239-253
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• 2005

한우의 혈액성분과 도체형질간의 상관관계를 구명하여 도체 품질을 예측하고 예견되는 능력에 따른 사양관리로 도체품질을 향상시키고자 한우에서 도축 2개월 전과 수송전 그리고 도살시 혈청성분 농도와 도체 형질간의 표현형 상관을 분석한 결과는 아래와 같다. 1. 거세우의 도살 2개월 전 혈청 성분 농도와 도체 형질간의 상관에서 육량지수는 total protein (0.23), albumin(0.26), calcium(0.31)과 정의상관이나 BUN (-0.30)과는 음의 상관관계가 있었으며, 배최장근단면적은 BUN(0.17), globulin(0.16)과 정의 상관이 있었다. 등지방두께는 BUN(0.42) 및 inorganig phosphorus (0.20)와 정의상관관계이나 total protein(-0.23), albumin(-0.33) 및 calcium(-0.33)과 음의 상관관계가 있었다. 그리고 근내지방도는 BUN (0.28)과는 정의 상관관계가 그리고 IGF-I 및 calcium과는 음의 상관관계가 있었다. 2 거세우의 수송전 혈청성분 농도와 도체형질간의 상관관계를 살펴보면, 배최장근단면적은 TP(-0.16) 및 BUN(-0.15)과 음의 상관이었고, 등지방두께는 IGF-I(-0.21), glucose (-0.21), creatinine(-0.19) 및 inorganic phosphorus (-0.18)와 음의 상관을 나타내었으며, 근내지 방도는 creatinine (-0.16)과 음의 상관관계가 있었다. 3, 거세우의 도살시 혈청 성분 농도와 도체형질간의 상관에서 육량지수는 연령을 보정한 calcium과 정의 상관(0.31)이었고, 생체중은 BUN(0.17)과 정의 상관이었으며, 배최장근단 면적은 albumin(-0.19)과 음의 상관을 나타내었고, 등지방두께는 연령으로 보정되어 calcium(-0.38)과 음의 상관이었고, 근내지방도는 연령으로 보정 한 calcium(-0.17)과 음의 상관을 나타내었다. 4. 도살 2개월 전 혈청 성분 농도와 도체 형질들간의 상관에서 육량지수는 testosterone 및 caicium과 0.71 및 0.67로 높은 상관이었고 inorganic phosphate와 -0.71의 높은 음의 상관을 보였다. 생체중은 calcium과 0.67의 높은 정의 상관이었으나, 도체중은 glucose와 -0.69의 음의 상관이었다. 등지방두께는 cortisol 및 inorganic phosphate(IP)와 0.29 및 0.69)로 정의 상관이었으며, 근내지방도는 creatinine과 -0.81로 높은 음의 상관이었으나 BUN과는 0.87로 높은 정의 상관관계를 나타내었다. 수송 감량은 albumin 및 inorganic phosphate와 0.77, 0.83로 높은 수준의 정의 상관관계를 나타내었다. 5. 비거세우의 수송전 혈청 성분 농도와 도체 형질들 간의 상관에서 육량지수는 testosterone과 0.66으로 정의 상관이었고, 도체율은 globulin과 0.73으로 높은 상관을 나타내었다. 등지방두께는 testosterone과 -0.60의 음의 상관이었고. 배최장근단면적은 testosterone과 0.40의 정의 상관이었고 근내지방도와 creatinine 농도간에는 -0.55의 비교적 높은 음의 상관계수가 추정되었으나 유의성은 인정되지 않았다. 6. 한우 비거세우의 도살시 혈청 성분 농도와 도체형질과의 상관에서 육량지수는 연령에 대해 보정한 HDLC 농도와 -0.71의 음의 상관이었으며, 도체율은 globulin과 0.70의 높은 정의 상관이었고, 등지방두께는 연령으로 보정된 HDLC와 0.69로 정의 상관관계를 보였다. 도체중은 triglyceride와 0.51의 정의 상관, 배최장근단면적은 testosterone과 -0.91, 근내지방도는 creatinine 및 glucose와 -0.58의 비교적 높은 상관을 보였으나 유의성이 인정되지 않았다.

• Childhood Kidney Diseases
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• 제11권1호
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• pp.16-23
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• 2007

목적 : 저감마글로불린혈증은 모든 신증후군에서 관찰되고 있으나 그 병태생리는 알려져 있지 않다. 미세 변화 신증후군 환아들의 혈청 IgG와 알부민 값 간의 상관관계를 분석하고자 하였다. 방법 : 신증후군으로 진단된 46명의 환아들(단백뇨 $>40mg/m^2/h$, 혈청 알부민 값 <2.5g/dL의 초발 입원 시의 IgG, 알부민 및 층 콜레스테롤 값을 취하고, 각 지표간의 상관관계를 통계학적으로 분석하였다. 결과 : 신증후군 환아들의 평균 알부민, IgG 및 총 콜레스테롤 값은 각각 $1.7{\pm}0.3g/dL,\;368{\pm}143mg/dL,\;431{\pm}78mg/dL$이었다. 혈청 알부민과 총 콜레스테롤 값의 관계에서, 두 지표는 서로 반비례를 보였다(r=0.58, P=0.0001), 한편 알부민과 IgG 값들 간의 관계에서 정비례 관계를 보였다. (r=0.37, P=0.01). 결론 : 신증후군에서 IgG 값은 알부민 값과 연관이 있으며, 소변 내 단백 손실의 정도를 반영한다. 이러한 현상을 밝히기 위한 추가 연구가 필요 할 것으로 보인다.

• 한국수정란이식학회지
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• 제18권3호
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• pp.215-225
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• 2003

본 시험은 한우에서 연령에 따라 혈청성분들의 변화를 알아보기 위하여 한우 866두(거세 638, 비거세 228)에 대하여 혈청 농도를 분석하여 채혈시 일령을 독립 변량으로 하고 혈청 성분들을 종속변수로 하는 다항 회귀방정식으로 추정한 결과는 다음과 같다. 거세우나 비거세우 모두 같은 차수의 회귀방정식이 접합한 혈청 성분은 IGF- I (3차식) calium(1차식) 및 IP(1차식)이었고 거세우에서는 1차식이 적합하고 비거세우에서는 3차식이 적합한 혈청 성분은 testosterone와 creatinine었다. 반면에 HDLC는 거세우에서는 3차식이 적합하나 비거세우에서는 1차식이 적합한 것으로 나타났다. 그리고 거세우에서는 2차식이 적합한데 비거세우에서는 3차식이 적합한 혈청성분은 triglyceride 농도와 globulin농도 그리고 A/G비율 등이었고, 거세우에서는 3차식이 적합하고 비거세우에서는 2차식이 적합한 혈청성분은 BUN이었으며, 거세우에서는 2차식이 적합한데 비거세우에서는 1차식이 적합한 혈청성분은 TP와 albumin이었다. 한편 cortisol은 거세우나 비거세우에서 모두 3차식까지의 회귀방정식으로는 연령에 따른 변화를 설명하기가 적합하지 않았으며 glucose는 비거세우에서는 3차식 변화를 보이고 있으나 거세우에서는 3차식까지의 회귀방정식만으로는 연령에 따른 변화를 설명하기가 어려웠다. 가장 적합한 것으로 판단되는 혈청성분들의 회귀모형 중에서 비교적 R-SQUARE 값이 높은 것(R-SQUARE value>0.1)들은 거세우에서 ICF-I, albumin, creatinine, IP, HDLC 등이었으며, 비거세우에서 testosterone, IGF-I, TP, albumin, glucose, creatinine, IP, HDLC 등으로 나타났다. 따라서 IGF-I, albumin, creatinine, IP, HDLC 등은 거세우나 비거세우 모두에서 연령에 따라 비교적 큰 변화를 보이는 혈청 성분이라고 생각된다.

• 대한핵의학회지
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• 제27권1호
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• pp.88-97
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• 1993

Anterior abdominal scintigraphy after intravenous injection of $^{99m}Tc$-human serum albumin ($^{99m}Tc$-HSA 20 mCi) was done in 16 patients with connective tissue diseases and 15 healthy control patients. Patients with proteinuria or hepatopathy were excluded. 1) 7 (44%) patients among 16 connective tissue disease patients without the apparent evidence of external protein loss showed abnormal intestinal accumulation of albumin. 6 patients with positive albumin scintigraphy showed bypoalbuminemia. 2) There was no false positive scintigraphic finding in control group. 3) The serum albumin level in connective tissue disease patients ($3.1{\pm}0.6 g/dl$, n=16) was lower than control patients ($3.9{\pm}0.3 g/dl$, n=15) (p<0.0001). 4) The serum albumin level was lower in connective tissue disease patients with positive $^{99m}Tc$-HSA scan ($2.8{\pm}0.6 g/dl$, n=7) than the connective tissue disease patients with negative scan ($3.3{\pm}0.3 g/dl$, n=9) (p<0.05). 5) The hemoglobin level in connective tissue disease patients with positive nan ($10.6{\pm}2.91 g/dl$) was lower than that of the control group ($13.6{\pm}1.5 g/dl$) (p<0.05). Mypoalbuminemia is frequently involved in chronic connective tissue diseases. Protein losing enteropathy (PLE) is also responsible for the majority of the bypoalbuminemia in these patients. But it has been ignored because the conventional method for the diagnosis of PLE was difficult to perform. $^{99m}Tc$-HSA scan also must be validated by more extended study and comparison with the quantitative study such as stool ${\alpha}-1$ antitrypsin measurement. There must be a reevaluation of PLE in various diseases especially in connective tissue diseases with easy, fast, economical, and non-invasive method.

• Clinical and Experimental Reproductive Medicine
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• 제22권2호
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• pp.171-181
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• 1995

Ovarian hyperstimulation syndrome(OHSS) is one of the well-known complication of controlled ovarian hyperstimulation(COH). Though there have been numerous measures to prevent the occurrence of OHS, it has not been completely preventable until now. The fluid shift from the intravascular space to the third space is due to decreased oncotic pressure of the serum. The objective of this study was to evaluate if IV administration of 20% albumin in those patients with OHSS risk can make prevention of severe OHSS. We retrospectively analysed 70 patients undergoing IVF-ET who had serum peak estradiol($E_2$) level of >2,500 pg/ml and/or the number of oocytes retrieved over 20. The treatment group(n=39) received albumin while the control group(n=31) did not. After 40 grams of human albumin diluted in 1,000 ml of 0.9% sodium chloride solution, the treatment group received half of the fluid during oocyte retrieval, the remainder in the recovery suite. The results were as follows; There were significant differences in the levels of serum peak $E_2$ and number of oocytes retrieved between the two groups(p<0.05). However, there were no significant differences in the incidence of OHSS and pregnancy rate or multifetal pregnancy rate. In conclusion, administration of albumin to OHSS risk patients did not reduce the rate of OHSS in IVF-ET. However, if we consider the fact that there were differences in the level of peak serum $E_2$ and oocyte numbers, further prospective study may be needed.

• 분석과학
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• 제36권1호
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• pp.12-21
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• 2023

Protein glycation is vital to aging and disease. However, glycated proteins are low-abundant in plasma, rendering them difficult to identify using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Many studies have analyzed glycated peptides with high reproducibility. Here, glycated peptides in human serum were analyzed by LC-MS/MS using data-dependent acquisition (DDA) and parallel reaction monitoring (PRM). Boronic acid (BA) enrichment of in vitro glycated human serum peptides was performed. BA enrichment identified the most glycated peptides, and the glycated peptides of the more diversified proteins, excluding albumin, were analyzed. In PRM, glycated albumin PSMs were the most common, and this method exhibited the best reproducibility. The results of this study could help compare methods for identifying glycation-related biomarkers.

• Korean Journal of Acupuncture
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• 제24권4호
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• pp.163-180
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• 2007

Objective : Oriental medicines have been applied to Membranous nephropathy(MN) for the purpose on increasing renal blood flow and modulating immune activity in according to Oriental medicine theories. Magsungsinyeom-bang (MSSYB) is one of the prescription which is known to show positive results in clinic with lack of laboratorial evidence. Thus, this study was aimed to evaluate the effects of MSSYB and partially investigate the mechanisms of it. Methods : The effect of MSSYB was evaluated by the morphology for the GBM thickening, protein excretion in urine and biochemical parameters in serum using cBSA-induced MN mice model. Mice were administered with MSSYB(250 or 500 mg/kg) or PBS for control group from experimental week 3 for 4 weeks. Results : 24 hrs proteinuria and the concentrations BUN was significantly decreased in the MS groups compared to the control group while the concentrations of serum albumin was higher in the MS groups than control group. MSSYB didn't affect the ratio of CD3e+/CD or 19CD4+/CD8 in the spleen and kidney, but inhibit the expression of IL-1${\beta}$, TNF-${\alpha}$, IL-6, and production of IgG and IgM. In histological analysis of kidney tissue, thickening of GBM was significantly decreased in the MS group compared to control group. Conclusions : MSSYB showed the positive results on the cBSA-induced membranous nephropathy in mice, thus, it could be a useful candidate for oriental drug for treating the membranous nephropathy in clinic.

• Journal of Community Nutrition
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• 제7권1호
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• pp.3-7
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• 2005

This study was conducted to evaluate nutritional status of school children by biochemical assessment method. Subjects were 308 school children from 4th to 6th grade living in Bucheon city. Their blood glucose, total protein, albumin, total cholesterol, and triglyceride (TG) concentrations were determined. Mean blood glucose concentration was 77.56 mg/dl and $81.2\%$ of children had normal blood glucose concentration. The average proportion of hypoglycemia was $18.8\%$ among the three grades and prevalence of hypoglycemia in 4th grade school children was highest ($21.8\%$). Mean serum total protein and albumin levels were 7.14g/dl and 4.35g/dl, respectively, and all the subjects except only one were in normal range of total protein and albumin. Mean serum TG and total cholesterol concentrations were 145.82mg/dl and 94.50mg/dl. Serum TG and total cholesterol levels showed same pattern that the concentrations decreased in proportion to grade. Especially, there was significant difference between grades in serum total cholesterol. Furthermore, $11.7\%$ of 4th, $6.8\%$ of 5th, and $2.8\%$ of 6th grade school children have borderline hypertriglycemia or hypertriglycemia, risk factors of obesity or cardiovascular disease later in life. Taken together, there was conflict phenomenon that undernutrition of hypertriglycemia and overnutrition of hypertriglycemia existed together. Therefore, it is needed to develop discriminating and personalized nutritional feeding, counseling, and education program to cover the children of under- or over- nutritional status.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권6호
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• pp.402-409
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• 2001

Different bioligands carrying synthetic adsorbents have been reported in the literature for protein separation, We have developed a novel and new approach to obtain high protein ad-sorption capacity utilizing 2-methacrylamidohistidine(MAH) as a bioligand. MAH was synthe-sized by reacting methacrylocholride and histidine, Spherical beads with an average size of 150-200㎛ were obtained by the radical suspension polymerization of MAH and 2-hydrosyethyl-methacrylate(HEMA) conducted in an aqueous dispersion medium. p(HEMA-co-MAH) beads had a specific surface area of 17.6㎡/g . Synthesized MAH monomer was characterized by NMR. p(HEMA-co-MAH) beads were characterized by swelling test, FTIR and elemental analysis. Then Cu(II) ions were incorporated onto the beads and Cu(II) loading was found to be 0.96 mmol/g.These affinity beads with a swelling ration of 65% and containing, 1.6 mmol MAH/g were used in the adsorption/desorption of human serum albumin(HSA) from both aqueous solutions and hu-man serum. The adsorption of HSA onto p(HEM-co-MAH) was low(8.8 mg/g). Cu(II) chelation onto the beads significantly increased the HSA adsorption (56.3 mg/g). The maximum HSA ad-sorption ws observed at pH 8.0 Higher HSA adsorption was observed from human plasma(94.6 mgHSA/g) Adsorption of other serum proteins were obtained as 3.7 mg/g for fibrinogen and 8.5mg/g for γ-globulin. The total protein adsorption was determined as 107.1mg/g. Desorption of HSA was obtained using 0.1 M Tris/HCl buffer containing 0.5 M NaSCN, High desorption rations(up to 98% of the adsorbed HSA) were observed. It was possible to reuse Cu(II) chelated-p(HEMA-co-MAH) beads without significant decreases in the adsorption capacities.