• BMB Reports
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• v.39 no.2
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• pp.150-157
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• 2006

Native grass carp (Ctenopharygodon idellus) growth hormone, has 5 cysteine amino acid residues, forms two disulphide bridges in its mature form. Recombinant grass carp growth hormone, when over-expressed in E. coli, forms inclusion bodies. In vitro oxidative renaturation of guanidine-hydrochloride dissolved recombinant grass carp growth hormone was achieved by sequential dilution and stepwise dialysis at pH 8.5. The redox potential of the refolding cocktail was maintained by glutathione disulphide/glutathione couple. The oxidative refolded protein is heterogeneous, and contains multimers, oligomers and monomers. The presence of non-disulphide-bond-forming cysteine in recombinant grass carp growth hormone enhances intermolecular disulphide bond formation and also non-native intramolecular disulphide bond formation during protein folding. The non-disulphide-bond-forming cysteine was converted to serine by PCR-mediated site-directed mutagenesis. The resulting 4-cysteine grass carp growth hormone has improved in vitro oxidative refolding properties when studied by gel filtration and reverse phase chromatography. The refolded 4-cysteine form has less hydrophobic aggregate and has only one monomeric isoform. Both refolded 4-cysteine and 5-cystiene forms are active in radioreceptor binding assay.

• BMB Reports
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• v.28 no.5
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• pp.451-457
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• 1995

Four carboxypeptidases, CP1, CP2, CP3, and CP4 were isolated from the cotyledons of germinating seedlings of Canavalia lineata by sequential chromatography on the following four columns: 1) CM-cellulose, 2) Sephacryl 5-300, 3) Procion red dye, and 4) Sephacryl S-200. A number of properties of the enzymes, such as substrate specificity, molecular weight, optimum pH, thermal stability, have been determined. Enzyme activities were measured using the Cbz(carbobenzoxy)-dipeptides containing phenylalanine at the penultimate position. The $K_m$ values of four carboxypeptidases for Cbz-Phe-Ala were 0.50, 0.65, 1.30, and 1.35 mM, respectively. The inhibition studies indicated that the four carboxypeptidases were all serine type. Each of the carboxypeptidases with molecular weights of 145, 114, 105, and 104 kDa, respectively, had the optimum enzyme activity at pH 5.0~6.0. And they were sensitive to high temperature.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.30 no.3
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• pp.185-191
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• 2017

Cadmium compounds with one dimension (1D) nanostructures have attracted attention for their excellent electrical and optical properties. In this study, vertically aligned CdTe-Si nanostructures with high density were synthesized by several simple chemical reactions. First, l D Te nanostructures were synthesized by silver assisted chemical Si wafer etching followed by a galvanic displacement reaction of the etched Si nanowires. Nanowire length was controlled from 1 to $25{\mu}m$ by adjusting etching time. The Si nanowire galvanic displacement reaction in $HTeO_2{^+}$ electrolyte created hybrid 1D Te-branched Si nanostructures. The sequential topochemical reaction resulted in $Ag_2Te-Si$ nanostructures, and the cation exchange reaction with the hybrid 1D Te-branched Si nanostructures resulted in CdTe-Si nanostructures. Wet chemical processes including metal assisted etching, galvanic displacement, topochemical and cation exchange reactions are proposed as simple routes to fabricate large scale, vertically aligned CdTe-Si hybrid nanostructures with high density.

• Environmental Engineering Research
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• v.17 no.3
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• pp.145-150
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• 2012

Pb-contaminated soil at a clay shooting range was analyzed by the sequential extraction method to identify metal binding properties in terms of detrital and non-detrital forms of the soil. Most of the metals in the soils existed as non-detrital forms, exchangeable and carbonate-bound forms, which could be easily released from the soil by a washing method. Therefore, the characteristics of Pb desorption for remediation of the Pb-contaminated soil were evaluated using hydrochloric acid (HCl) by a washing method. Batch experiments were performed to identify the factors influencing extraction efficiency. The effects of the solid to liquid (S/L) ratio (1:2, 1:3, and 1:4), soil particle size, and extraction time on the removal capacity of Pb by HCl were evaluated. Soil samples were collected from two different areas: a slope area (SA) and a land area (LA) at the field. As results, the optimal conditions at 2.8 to 0.075 mm of particle size were 1:3 of the S/L ratio and 10 min of extraction time for SA, and 1:4 of the S/L ratio and 5 min of extraction time for LA. The characteristics of Pb desorption were adequately described by two-reaction kinetic models.

• Proceedings of the KOSOMBE Conference
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• v.1995 no.11
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• pp.253-256
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• 1995

Polymers have been one of the emerging biomedical materials in the area of biomedical research which are applicable to the human body. For human applications, noninvasive characterization of the biomedical polymers has been one of the important topics, and is valuable information. Among others, the swelling rate is one of the important measurements needed for the hydrophilic polymers. NMR imaging has been a suitable method for the noninvasive study of such a material since it is sensitive to many physical and biochemical changes of the specimens. In addition, NMR techniques possess many useful intrinsic properties such as the relaxation and diffusion effects. The present study has provided a noble and noninvasive method of measuring the process of swelling as well as volumetric changes occurred in polymers and drug delivery processes in a drug delivery system (DDS) together with changes of released drug. This gives information, relating with both water ingress process, volumetric changes of polymer specimens and the visualization of sequential drug delivery process. Also, this study provides more reliable method to ascertain the time dependent swelling process compared to the conventional method. The important aspects is that the proposed method is truly noninvasive and is able to ascertain time dependent processes.

• Journal of Information Technology and Architecture
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• v.11 no.1
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• pp.25-32
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• 2014

Web mining techniques are often used to discover useful patterns from data log generated by Web servers for the purpose of web usage analysis. Yet traditional Web mining techniques do not reflect sufficiently sequential properties of Web log data. To address such weakness, we introduce a framework for analyzing Web access log data by using process mining techniques. To illustrate the proposed framework, we show the analysis of Web access log in a campus information system based on the framework and discuss the implication of the analysis result.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1995.11a
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• pp.109-112
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• 1995

CuInS$_2$thin film was prepared by heat treatment at vacuum 10$\^$-3/ Torr of S/In/Cu stacked layer which was deposited by sequential. And so, the polycrystalline CuInS$_2$with chalcopyrite structure was well made at heat treatment temperature of 250$^{\circ}C$ and heat treatment time of 60 min. Single phase of CuInS$_2$was formed from Cu/In composition ratio of 0.84 to 1.3. p conduction type of CuInS$_2$thin film was appeared from Cu/In competition ratio of 0.99. The highest resistivity of CuInS$_2$with p type was 1.608${\times}$10$^2$$\Omega$cm at Cu/In composition ratio of 0.99 and The lowest resistivity was 5.587${\times}$10$\^$-2/$\Omega$cm at Cu/In composition ratio of 1.3.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2002.07b
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• pp.939-944
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• 2002

PD phenomena can be regarded as a deterministic dynamical process where PD should be occurred if the local electric field be reached to be sufficiently high. And thus, its mathematical model can be described by either difference equations or differential equations using several state variables obtained from the time sequential measured data of PD signals. These variables can provide rich and complex behavior of detectable time series, for which Chaos theory can be employed. In this respect, a new PD pattern recognition method is proposed and named as 'Chaotic Analysis of Partial Discharges (CAPD)' for this work. For this purpose, six types of specimen are designed and made as the models of the possible defects that may cause sudden failures of the underground power transmission cables under service, and partial discharge signals, generated from those samples, are detected and then analyzed by means of CAPD. Throughout the work, qualitative and quantitative properties related to the PD signals from different defects are analyzed by use of attractor in phase space, information dimensions ($D_0$ and D2), Lyapunov exponents and K-S entropy as well. Based on these results, it could be pointed out that the nature of defect seems to be identified more distinctively when the CAPD is combined with traditional statistical method such as PRPDA. Furthermore, the relationship between PD magnitude and the occurrence timing is investigated with a view to simulating PD phenomena.

• Computers and Concrete
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• v.6 no.2
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• pp.133-153
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• 2009

Discrete element modeling (DEM) in concrete technology is concerned with design and use of models that constitute a schematization of reality with operational potentials. This paper discusses the material science principles governing the design of DEM systems and evaluates the consequences for their operational potentials. It surveys the two families in physical discrete element modeling in concrete technology, only touching upon probabilistic DEM concepts as alternatives. Many common DEM systems are based on random sequential addition (RSA) procedures; their operational potentials are limited to low configuration-sensitivity features of material structure, underlying material performance characteristics of low structure-sensitivity. The second family of DEM systems employs concurrent algorithms, involving particle interaction mechanisms. Static and dynamic solutions are realized to solve particle overlap. This second family offers a far more realistic schematization of reality as to particle configuration. The operational potentials of this family involve valid approaches to structure-sensitive mechanical or durability properties. Illustrative 2D examples of fresh cement particle packing and pore formation during maturation are elaborated to demonstrate this. Mainstream fields of present day and expected application of DEM are sketched. Violation of the scientific knowledge of to day underlying these operational potentials will give rise to unreliable solutions.

• JSTS:Journal of Semiconductor Technology and Science
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• v.17 no.1
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• pp.65-78
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• 2017

Single Event Transient has a critical impact on highly integrated logic circuits which are currently common in various commercial and consumer electronic devices. Reliability against the soft and intermittent faults will become a key metric to evaluate such complex system on chip designs. Our previous work analyzing soft errors was focused on parallelizing and optimizing error propagation procedures for individual transient faults on logic and sequential cells. In this paper, we present a new propagation technique where a fault binary decision diagram (BDD) continues to merge every new fault generated from the subsequent logic gate traversal. BDD-based transient fault analysis has been known to provide the most accurate results that consider both electrical and logical properties for the given design. However, it suffers from a limitation in storing and handling BDDs that can be increased in size and operations by the exponential order. On the other hand, the proposed method requires only a visit to each logic gate traversal and unnecessary BDDs can be removed or reduced. This results in an approximately 20-200 fold speed increase while the existing parallelized procedure is only 3-4 times faster than the baseline algorithm.