• Journal of fish pathology
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• v.24 no.3
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• pp.297-305
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• 2011

A trap identification system for isolating functional sequences to allow the constitutive expression of foreign protein from Edwardsiella tarda was developed. Using the green fluorescent protein (GFP) reporter-based trap system, various functional sequences to drive heterologous expression of the GFP were selectable in Escherichia coli host. However from the bioinformatic sequence analysis, all the segments predicted as regulatory regions were not native promoters actually existing upstream of endogenous E. tarda genes. Instead, a number of non-authentic sequences, possibly resulted from the random shuffling and/or intermolecular ligation were also proven to be able to display a potent GFP expression in the recombinant E. coli. Further analysis with selected clones showed that both authentic and non-authentic sequences could function in as a constitutive promoter, leading quite a consistent and stable GFP expression after repetitive subcultures. Microscopic examination also confirmed the uniform pattern of GFP expression in every host bacterium. Semi-quantitative assay of GFP showed that there was no clear relationship between expression levels and organizational features of the promoters trapped. Functional promoter-like elements achieved in the present study could be a good starting material for multivalent genetic engineering of E. tarda in order to produce recombinant vaccines in a cost-effective fashion.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.4
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• pp.306-308
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• 2001

A recombinant human growth hormone(hGH) was expressed as a secretory product in the yeast Saccharomyuces cerevisiae. There different leader sequences derived from the mating fac-tor $\alpha$1(MF$\alpha$1) inulinase and invertase were used to direct the secretion of hGH into the extracel-lular medium. Among three leader sequences tested, the inulinase leader sequence was found to be the most efficient in the secretory expression of hGH. In contrast, no hGH was detected in the ex-tracellular medium with the invertase leader sequence. After 48 h shake-flask culture, the yields of hGH secreted into th emedium by the invertase. MF$\alpha$1 inulinase and invertase leader sequences were approximately 0, 0.3 and 0.9 mg/L, respectively. The secretion efficiencies were also found to be 0, 3.8 and 13% for the invertase , MG$\alpha$1 and inulinase leader sequences, respectively.

• The Journal of the Korea institute of electronic communication sciences
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• v.11 no.9
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• pp.885-892
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• 2016

The nonlinear sequence generator called the shrinking generator was designed as nonlinear keystream generator composed by two maximum-length LFSRs. The shrunken sequences generated by the shrinking generator are included in the class of interleaved sequences and can be modelled as one of the output sequences of cellular automata (CA). In this paper, we propose a method for synthesizing a 90/150 CA-based sequence generator to generate a family of sequences with the same characteristic polynomial as the shrunken sequences.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.3 no.4
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• pp.805-817
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• 1999

In this paper, the needs for introduction and adoption of MㆍHF DSC/NBDP system and for developments of its circuits and call sequences for use in the maritime mobile services for small-ships, leisure-ships and fishing ships are analyzed, discussed. Also design and implement for MㆍHF(1.6-4MHz) DSC/NBDP system is discussed. Most of casualties have been arisen from small-ships and fishing ships during last 5 years. So, the SAR schematic plans should been prepared to prevent casualties and facilitate the activities of SAR for those ships. DSC/NBDP for MㆍHF system is able to fulfill the roles of efficient SAR communication functions, and to advance the SAR system to small ships and fishing ships. This study is focused on the techniques of processing the DSC call sequences and the ARQ sequences of NBDP system. Especially ARQ sequences are expanded into processing of Korean letters, designed the call sequences and code conversion algorithm for Korean-code. It will be evaluated the availability of Korean-NBDP system. In designing the Transmitting circuits and Receiving circuits, for the carrier generation, DDS(Direct Digital Synthesizer) is used in stead of the Phase Locked Loop and frequency conversion by the mixer, BPF. And PSK modulation signals are directly generated by the controls of DDS, which show the characteristics of Spurious Free Dynamic Range are below -62dBc. Also, the monolithic U subsystem IC which provides various functional components, AD608 is used for designing the receiving circuitsㆍAnd the algorithm of Phasing methode for FSK demodulation are devised to process IF frequency 455kHz in the IF circuits.

• Interdisciplinary Bio Central
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• v.4 no.4
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• pp.14.1-14.6
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• 2012

Splice site prediction in DNA sequence is a basic search problem for finding exon/intron and intron/exon boundaries. Removing introns and then joining the exons together forms the mRNA sequence. These sequences are the input of the translation process. It is a necessary step in the central dogma of molecular biology. The main task of splice site prediction is to find out the exact GT and AG ended sequences. Then it identifies the true and false GT and AG ended sequences among those candidate sequences. In this paper, we survey research works on splice site prediction based on support vector machine (SVM). The basic difference between these research works is nucleotide encoding technique and SVM kernel selection. Some methods encode the DNA sequence in a sparse way whereas others encode in a probabilistic manner. The encoded sequences serve as input of SVM. The task of SVM is to classify them using its learning model. The accuracy of classification largely depends on the proper kernel selection for sequence data as well as a selection of kernel parameter. We observe each encoding technique and classify them according to their similarity. Then we discuss about kernel and their parameter selection. Our survey paper provides a basic understanding of encoding approaches and proper kernel selection of SVM for splice site prediction.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.29 no.2
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• pp.36-45
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• 1997

Newly bleaching sequence using oxygen, ozone and hydrogen peroxide has introduced to avoid pollution hazards from chlorinated organic compounds, because chlorine dioxide substitution bleaching was produced a little chlorinated organic substance. Oxygen-type chemicals replaced for chlorine has attracted much research attention. Bleachability of ozone was improved at low temperature and high pulp consistency. In third bleaching followed OZ bleaching, addition of O2 and NaBH4 in alkali extraction was effective than only alkali extraction. Bleachability of pulps in ozone bleaching(Z) was improved at higher consistency and lower temperature The addition O2 and NaBH4 in alkali extraction after OZ bleaching sequence improved brightness, when compared to those obtained by only alkaline extraction. Pulps bleached by ECF bleaching sequences such as OZEoD and OZEopD was obtained by 90% ISO brightness. The brightness of pulp bleached by TCF sequences with the ozone dosage of 1.6% was approached to target brightness (88~90%ISO). Pulps bleached Z stage combined bleaching sequence was reduced the viscosity to a little greater extent. However, physical properties of pulps was not great different compared to those bleached by conventional bleaching sequences. A pollution index of bleaching effluente by ozone combined bleaching sequences was lower than by conventional bleaching sequence, but somewhat higher than multistage bleaching sequences combined C/D stage.

• Journal of Microbiology and Biotechnology
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• v.24 no.10
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• pp.1301-1307
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• 2014

White-rot fungi of the genus Bjerkandera are cosmopolitan and have shown potential for industrial application and bioremediation. When distinguishing morphological characters are no longer present (e.g., cultures or dried specimen fragments), characterizing true sequences of Bjerkandera is crucial for accurate identification and application of the species. To build a framework for molecular identification of Bjerkandera, we carefully identified specimens of B. adusta and B. fumosa from Korea based on morphological characters, followed by sequencing the internal transcribed spacer region and 28S nuclear ribosomal large subunit. The phylogenetic analysis of Korean Bjerkandera specimens showed clear genetic differentiation between the two species. Using this phylogeny as a framework, we examined the identification accuracy of sequences available in GenBank. Analyses revealed that many Bjerkandera sequences in the database are either misidentified or unidentified. This study provides robust reference sequences for sequence-based identification of Bjerkandera, and further demonstrates the presence and dangers of incorrect sequences in GenBank.

• Korean Journal of Veterinary Research
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• v.38 no.2
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• pp.319-327
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• 1998

The nucleotide sequences of spike (S) glycoprotein containing antigenic sites A and D of TGEV isolated in Korea were determined and compared with published sequences for TGEVs. The TGEV 133 and DAE5 strains had 97.40% nucleotide sequence similarity. The overall nucleotide sequence similarity of the 133 and DAE5 strains compared with other TGEV strains was between 96.86% and 99.15%. The similarity of the predicted amino acid sequence of the 133 and DAE5 strains was 94.93%. The TGEV 133 and DAE5 strains had 94.93-98.61% amino acid similarity with published sequences of other TGEV strains. The sequences of amino acid codons in the antigenic sites A and D were identical among all the viruses although there were several nucleotide changes in region containing antigenic sites A and D of Korean TGEV isolates. By phylogenetic analysis of the sequences, two Korean isolates 133 and DAE5 seemed to be derived from different lineages. These studies showed that a distinct difference in genome exists among TGEV field strains isolated in Korea.

• Korean Journal of Veterinary Research
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• v.48 no.3
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• pp.287-293
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• 2008

The cDNA of the aquabirnavirus, GC-1 isolated from rockfish Sebastes schlegeli in Korea, was synthesized using the reverse transcriptase-polymerase chain reaction. The nucleotide and deduced amino acid sequences were determined from cDNA of the VP2-NS-VP3 coding region of genome segment A. The nucleotide sequences of the segment A were 3,086 base pairs (bp) in length and contained large open reading frame (ORF) and terminal sequences. The large ORF was comprised of 2,916 bp nucleotides and composed of 972 deduced amino acid sequences. Pairwise comparisons were made with other aquabirnavirus sequences published previously. The study of genetic relationships between GC-1 and aquabirnaviruses in the large ORF and VP2 coding regions demonstrated that the GC-1 has the nearest genetic relationship with the marine birnaviruses (MABV strains), and the GC-1 and MABV strains can be clustered as the same genogroup. GC-1 can be included in MABV, which is the 7th genogroup of family Aquabirnaviridae.

• 제어로봇시스템학회:학술대회논문집
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• 1993.10a
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• pp.233-238
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• 1993

In this paper, two diagnosis algorithms using the simple fuzzy, cognitive map (FCM) that is an useful qualitative model are proposed. The first basic algorithm is considered as a simple transition of Shiozaki's signed directed graph approach to FCM framework. And the second one is an extended version of the basic algorithm. In the extension, three important concepts, modified temporal associative memory (TAM) recall, temporal pattern matching algorithm and hierarchical decomposition are adopted. As the resultant diagnosis scheme takes short computation time, it can be used for on-line fault diagnosis of large scale and complex processes that conventional diagnosis methods cannot be applied. The diagnosis system can be trained by the basic algorithm and generates FCM model for every experienced process fault. In on-line application, the self-generated fault model FCM generates predicted pattern sequences, which are compared with observed pattern sequences to declare the origin of fault. In practical case, observed pattern sequences depend on transport time. So if predicted pattern sequences are different from observed ones, the time weighted FCM with transport delay can be used to generate predicted ones. The fault diagnosis procedure can be completed during the actual propagation since pattern sequences of tvo different faults do not coincide in general.