• Title/Summary/Keyword: sequence of sets

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Evaluation of Germplasm and Development of SSR Markers for Marker-assisted Backcross in Tomato (분자마커 이용 여교잡 육종을 위한 토마토 유전자원 평가 및 SSR 마커 개발)

  • Hwang, Ji-Hyun;Kim, Hyuk-Jun;Chae, Young;Choi, Hak-Soon;Kim, Myung-Kwon;Park, Young-Hoon
    • Horticultural Science & Technology
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    • v.30 no.5
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    • pp.557-567
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    • 2012
  • This study was conducted to achieve basal information for the development of tomato cultivars with disease resistances through marker-assisted backcross (MAB). Ten inbred lines with TYLCV, late blight, bacterial wilt, or powdery mildew resistance and four adapted inbred lines with superior horticultural traits were collected, which can be useful as the donor parents and recurrent parents in MAB, respectively. Inbred lines collected were evaluated by molecular markers and bioassay for confirming their disease resistances. To develop DNA markers for selecting recurrent parent genome (background selection) in MAB, a total of 108 simple sequence repeat (SSR) primer sets (nine per chromosome at average) were selected from the tomato reference genetic maps posted on SOL Genomics Network. Genetic similarity and relationships among the inbred lines were assessed using a total of 303 polymorphic SSR markers. Similarity coefficient ranged from 0.33 to 0.80; the highest similarity coefficient (0.80) was found between bacterial wilt-resistant donor lines '10BA333' and '10BA424', and the lowest (0.33) between a late blight resistant-wild species L3708 (S. pimpinelliforium L.) and '10BA424'. UPGMA analysis grouped the inbred lines into three clusters based on the similarity coefficient 0.58. Most of the donor lines of the same resistance were closely related, indicating the possibility that these lines were developed using a common resistance source. Parent combinations (donor parent ${\times}$ recurrent parent) showing appropriate levels of genetic distance and SSR marker polymorphism for MAB were selected based on the dendrogram. These combinations included 'TYR1' ${\times}$ 'RPL1' for TYLCV, '10BA333' or '10BA424' ${\times}$ 'RPL2' for bacterial wilt, and 'KNU12' ${\times}$ 'AV107-4' or 'RPL2' for powdery mildew. For late blight, the wild species resistant line 'L3708' was distantly related to all recurrent parental lines, and a suitable parent combination for MAB was 'L3708' ${\times}$ 'AV107-4', which showed a similarity coefficient of 0.41 and 45 polymorphic SSR markers.

Formation and Evolution of the Paleo-Seomjin River Incised-Valley System, Southern Coast of Korea: 1. Sequence Stratigraphy of Late Quaternary Sediments in Yosu Strait (한반도 남해안 고섬진강 절개곡 시스템의 형성과 진화: 1. 여수해협의 후기 제 4기층에 대한 순차층서)

  • Chun, Seung-Soo;Chang, Jin-Ho
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.6 no.3
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    • pp.142-151
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    • 2001
  • Detailed interpretation of some high-resolution seismic profiles in Yosu Strait reveals that Late Quaternary deposits consist of three allostratigraphic units (UH, LH, PL) formed by fluvial and tidal controls. The top mud unit, UH, thins onshore, and overlies the backstepping modem Seomjin delta deposits, which is interpreted as a transgressive systems tract (757) related to Holocene relative sea-level rise. The unit LH below the unit UH is composed of delta, valley- and basin-fill facies. The delta facies (Unit $LH_1$) occurs only in Gwangyang Bay and shows two prograding sets retrogradationaly stacked, thus it is also interpreted as a transgressive systems tract(757). On the contrary, the valley- and basin-fill facies (Unit $LH_2$), interpreted as 757, occur between the units UH and PL (Pleistocene deposits) in Yosu Strait. The bounding surface between UH and $LH_2$ can be interpreted as a tidal ravinement surface on the basis of trends thinning toward inner bay and becoming young landward. Furthermore its geomorphological pattern is similar to that of recent tidal channels. This allostratigraphy in'ffsu Strait suggests that two 757 deposits (UH and $LH_2$), divided by tidal ravinement surface, have been formed in Yosu Strait, whereas in Gwangyang Bay backstepping delta deposits ($LH_1$) without tidal ravinement surface have been formed during Holocene sea-level rise. These characteristics indicate that different stacking patterns could be formed in these two areas according to different increasing rate of accommodation space caused by different geomorphology, sediment supply and tidal-current patterns even in the same period of Holocene sea-level rise.

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