• Horticultural Science & Technology
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• v.29 no.2
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• pp.116-122
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• 2011

In this study, we report the generation and analysis of 1,392 expressed sequence tags (ESTs) from Korean Stewartia (Stewartia koreana Nakai). A cDNA library was generated from the young leaf tissue and a total of 1,392 cDNA were partially sequenced. EST and unigene sequence quality were determined by computational filtering, manual review, and BLAST analyses. Finally, 1,301 ESTs were acquired after the removal of the vector sequence and filtering over a minimum length 100 nucleotides. A total of 893 unigene, consisting of 150 contigs and 743 singletons, was identified after assembling. Also, we identified 95 new microsatellite-containing sequences from the unigenes and classified the structure according to their repeat unit. According to homology search with BLASTX against the NCBI database, 65% of ESTs were homologous with known function and 11.6% of ESTs were matched with putative or unknown function. The remaining 23.2% of ESTs showed no significant similarity to any protein sequences found in the public database. Annotation based searches against multiple databases including wine grape and populus sequences helped to identify putative functions of ESTs and unigenes. Gene ontology (GO) classification showed that the most abundant GO terms were transport, nucleotide binding, plastid, in terms biological process, molecular function and cellular component, respectively. The sequence data will be used to characterize potential roles of new genes in Stewartia and provided for the useful tools as a genetic resource.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.6
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• pp.821-826
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• 2007

The aim of the experiment was to detect polymorphisms in the keratin-associated protein 8.2 (KAP8.2) gene to determine associations between the genotype and fibre traits in Chinese Inner Mongolia cashmere goats. The fibre traits data investigated were cashmere fibre diameter, combed cashmere weight, cashmere fibre length and guard hair length. Five hundred and forty-two animals were used to detect polymorphisms in the complete coding sequence of the hircine KAP8.2 gene by means of PCR-SSCP. The results identified six genotypes, AA, BB, DD, AB, AD and BD, coded for by three different alleles A, B and D. Two SNPs in the coding region were confirmed by sequencing, which were A214G and T218C respectively. The relationships between the genotypes and cashmere fibre diameter, combed cashmere weight, cashmere fibre length and guard hair length were analyzed. There were significant differences (p<0.01) between the associations of the different genotypes with cashmere fibre diameter, cashmere weight and hair length. Cashmere length was the only trait that was not associated with the genotypes. The genotype AA (0.73) was found to be predominant in Inner Mongolia cashmere goats and the animals with this genotype had the thinnest cashmere fibre diameter compared with the other genotypes. These results suggested that polymorphisms in the hircine KAP8.2 gene may be a potential molecular marker for cashmere fibre diameter in cashmere goats.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.10
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• pp.1341-1347
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• 2011

The objective of this study was to investigate polymorphisms of keratin-associated protein 8.1 (KAP8.1) gene and its effect on fibre traits of Chinese Inner Mongolian Cashmere goats. The fibre traits data investigated were cashmere fibre diameter, combed cashmere weight, cashmere fibre length and guard hair length. Five hundred and forty animals were used to detect polymorphisms in the complete coding sequence of the hircine KAP8.1 gene by means of PCR-SSCP. The results identified six genotypes, AA, BB, CC, AB, AC and BC, coded for by three different alleles A, B and C. Two SNPs in the coding region were confirmed by sequencing, which were T113G and G116C respectively. The relationships between the genotypes and cashmere fibre diameter, combed cashmere weight, cashmere fibre length and guard hair length were analyzed. There were significant differences between the associations of the different genotypes with cashmere weight (p<0.01), cashmere length (p<0.05) and hair length (p<0.01). Cashmere fibre diameter was the only trait that was not associated with the genotypes. The animals of genotype AB and BB had the higher cashmere weight compared with the genotype AA. By further analysis, it appeared that the KAP8.1 genotype effects on fibre traits may be due to a mutation at the 113 locus. These results suggested that polymorphisms in the hircine KAP8.1 gene might be a potential molecular marker for cashmere weight in Cashmere goats.

• Animal cells and systems
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• v.5 no.4
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• pp.313-317
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• 2001

The sequential assessment model describes a fight between two conspecific as an ongoing statistical sampling process, which makes it possible to predict fight length or repetition number of a behavioral element depending on relative RHP (resource holding potential: e.g. weight or fighting ability). We staged contests between males of common freshwater gobies to test some predictions of this model. Fights proceeded in a consistent sequence of phases. Most contests began with two contestants adopting lateral display, and then escalated to intense physical contacts. The length of contests was negatively correlated with weight difference between the contestants. The duration of complete phases was, however, independent of weight, and the prior information gained during complete phases did not appear to affect subsequent phases of the fight. Our results show that the contests of common freshwater gobies are well predicted by the sequential assessment model.

• The Plant Pathology Journal
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• v.22 no.3
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• pp.235-238
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• 2006

Chrysanthemum stunt viroid (CSVd) induced systemic symptoms on chrysanthemum. We detected small RNAs of approximately 22 nucleotides with sequence specificity to CSVd in chrysanthemum infected with CSVd: an indication of the presence of RNA silencing. Regardless of symptom differences associated with CSVd, the small RNAs distributed similarly in amount. Small RNAs were detected with partial-length or full-length probes, indicating that they are not restricted to specific viroid regions but likely representing most of the viroid molecule.

• Journal of Korean Physical Therapy Science
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• v.5 no.1
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• pp.573-581
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• 1998

Gait is a highly complex activity in which many variables can be observed and measured. Walking is a repetitious sequence of limb to move the body and to maintain stability. Normal gait is rhythmic and characterized by alternating propulsive and retropulsive motions of the lower extremities. Pathological gait patterns have four functional categories (deformity, muscle weakness, impaired control, pain). The purpose of this study was to assess the quantitive gait variables(the width of the base, length of a step, stride length, cadence, velocity) in patients with low back pain. Patients walked more slowly, took shorter steps and did not show the symmetrical gait patterns.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.9 no.4
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• pp.34-47
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• 2000

Since carbon fiber epoxy composite materials have excellent properties for structures due to their high specific strength, high specific modulus, high damping and low thermal expansion, the hollow shafts made of carbon fiber epoxy composites have been widely used for power transmission shafts for motor vehicles , spindles of machine tools, motor base, bearing mount for tool up and manufacturing. The molded composite machine elements are not usually accurate enough for mechanical machine elements, which require turning drilling , cutting and grinding. The experiment are surface grinding wheel GC60 to the carbon fiber epoxy composite specimen with respect to staking angle [0]nT , [45]nT, [90]nT on the CNC grinding machine. In this paper, the surface grinding characteristics of composite plate, which are surveyed experimentally and analytically with respect to the grinding force, surface roughness and wheel loading according to the variable depth of cut, wheel velocity and table feed rate are investigated.

• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1935-1939
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• 2006

The gene encoding the extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA) depolymerase of Pseudomonas luteola Ml3-4, $phaZ_{plu}$, was cloned and analyzed. It was found to be 849 bp, with a deduced protein of 282 amino acids, and was revealed to have a typical leader peptide at its N terminus. The amino acid sequence of $PhaZ_{plu}$ revealed relatively low identity (69 to 72%) with those of other Pseudomonas MCL-PHA depolymerases. In comparison with the amino acid sequences of all available MCL-PHA depolymerases, the depolymerase was found to consist of three domains in sequential order; signal peptide, an N-terminal substrate binding domain, and a catalytic domain, indicating that $PhaZ_{plu}$ belongs to the type IV depolymerases family. The enzyme also contained Asn as an oxyanion hole amino acid.

• Journal of Computing Science and Engineering
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• v.10 no.1
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• pp.9-20
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• 2016

Several task clustering heuristics are proposed for allocating tasks in heterogeneous systems to achieve a good response time in data intensive jobs. However, one of the challenging problems is the process in task scheduling after task allocation by task clustering. We propose a task scheduling method after task clustering, leveraging worst schedule length (WSL) as an upper bound of the schedule length. In our proposed method, a task in a WSL sequence is scheduled preferentially to make the WSL smaller. Experimental results by simulation show that the response time is improved in several task clustering heuristics. In particular, our proposed scheduling method with the task clustering outperforms conventional list-based task scheduling methods.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.21 no.48
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• pp.299-310
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• 1998

Actually mixed assembly line is mixed with open and close type workstation. This workstation is called hybridworkstation. The propose of this paper is to determine the sequencing of model that minimize line length for actual(hybridworkstation) mixed model assembly line. we developed three mathematical formulation of the problem to minimize the overall length of a line with hybrid station. Mathematical formulation classified model by operato schedule. Mixed model assembly line is combination program and NP-hard program. Thus computation time is often a critical factor in choosing a method of determining the sequence. This study suggests a tabu search technique which can provide a near optimal solution in real time and use the hill climbing heuristic method for selecting initial solution. Modified tabu search method is compared with MIP(Mixed Integer Program). Numerical results are reported to demonstrate the efficiency of the method.