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Cloning and Analysis of Medium-Chain-Length Poly(3-Hydroxyalkanoate) Depolymerase Gene of Pseudomonas luteola M13-4  

Park, In-Jae (Department of Microbiology, Daejeon University)
Rhee, Young-Ha (Department of Microbiology, Chungnam National University)
Cho, Nam-Young (Department of Microbiology, Daejeon University)
Shin, Kwang-Soo (Department of Microbiology, Daejeon University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.12, 2006 , pp. 1935-1939 More about this Journal
Abstract
The gene encoding the extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA) depolymerase of Pseudomonas luteola Ml3-4, $phaZ_{plu}$, was cloned and analyzed. It was found to be 849 bp, with a deduced protein of 282 amino acids, and was revealed to have a typical leader peptide at its N terminus. The amino acid sequence of $PhaZ_{plu}$ revealed relatively low identity (69 to 72%) with those of other Pseudomonas MCL-PHA depolymerases. In comparison with the amino acid sequences of all available MCL-PHA depolymerases, the depolymerase was found to consist of three domains in sequential order; signal peptide, an N-terminal substrate binding domain, and a catalytic domain, indicating that $PhaZ_{plu}$ belongs to the type IV depolymerases family. The enzyme also contained Asn as an oxyanion hole amino acid.
Keywords
Molecular analysis; MCL-PHA depolymerase gene; Pseudomonas luteola M13-4;
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Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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