• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.387-395
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• 2018

This study was conducted to identify DNA markers related to resistance to herbicide containing mesotrione in Tongil type rice. Two Tongil type elite lines; Milyang154 and Suweon382, showed resistance to mesotrione, whereas the others were susceptible at 20 days after mesotrione application, and severe growth inhibition was observed in the remaining 13 lines. As a result of analysis of mesotrione resistance using 190 $F_2$ populations derived from a cross of Hanareum2 (susceptible) and Milyang154 (resistant), the mesotrione resistance locus was shown to be a single dominant gene with a 3:1 segregation ratio ($X^2=1.19$, P=0.31). To identify a DNA marker closely linked to the mesotrione resistance gene, bulked segregant analysis (BSA) was adopted. The DNA marker RM3501 was identified on chromosome 2 with a recombinant value of 0.53 to the mesotrione resistance gene. Mst1(t) was located between SSR (simple sequence repeat) markers RM3501 and RM324 with a physical map distance of 10.2 Mb-11.4 Mb on chromosome 2. The band pattern of agarose gel electrophoresis of the SSR marker RM3501 showed the same segregation pattern with respect to mesotrione treatment in 20 Tongil type varieties and a $BC_2F_2$ segregation population derived from a cross between Unkwang (resistant) and Hanareum2 (susceptible). Thus, the RM3501 DNA marker could be used in breeding programs for Marker Assisted Selection in mesotrione resistant rice breeding.

• Journal of fish pathology
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• v.33 no.2
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• pp.111-118
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• 2020

The genus Edwardsiella belonging to the family Enterobacteriaceae is a member of Gram-negative rod-shaped bacteria that cause disease in diverse aquatic organisms such as fish, amphibians and reptiles as well as avians and mammals including human throughout the world. This genus had been composed of three species, E. hoshinae, E. ictaluri and E. tarda, but recent researches erected two novel species, E. anguillarum and E. piscicida that were conventionally identified as E. tarda. In this study, we isolated seven strains belonging to the genus Edwardsiella from freshwater fishes that had been reared at inland fish farms in South Korea and investigated their biochemical characteristics and molecular phylogenetic relationships. The seven strains showed typical characteristics of four Edwardsiella species, E. anguillarum, E. ictaluri, E. piscicida and E. tarda, by biochemical analyses of Gram staining, indole and hydrogen sulfide (H2S) production, and API (Analytic Profile Index) 20E test. Molecular phylogenetic analyses inferred from DNA sequence data of both 16S ribosomal RNA (rRNA) and DNA gyrase subunit B (gyrB) genes were congruent with the biochemical characteristics. As a result, both biochemical and molecular phylogenetic analyses identified four strains isolated from three Anguilla species as E. anguillarum, E. piscicida and E. tarda, two strains from Pelteobagrus fulvidraco and Silurus asotus as E. ictaluri, and one strain from Moroco oxycephalus as E. piscicida. In this study, we isolated and successfully identified recently newly erected species, E. anguillarum and E. piscicida in addition to historically notorious pathogenic species, E. ictaluri and E. tarda. In the future study, systematic and comprehensive monitoring of the four Edwardsiella species are required for studying differences in pathogenicity among freshwater fishes.

• Research in Plant Disease
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• v.27 no.4
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• pp.172-179
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• 2021

This study aimed to assess the disease incidence and distribution of toxigenic in Korean triticale. The pathogen of triticale that cause Fusarium head blight were isolated from five different triticale cultivars that cultivated in Suwon Korea at 2021 year. The 72 candidate were classified as a Fusarium asiaticum by morphology analysis and by ITS1, TEF-1α gene sequence analysis. And the results of pathogenicity with 72 isolates on seedling triticale, 71 isolates were showed disease symptom. Also, seven out of 71 Fusarium isolates were inoculated on the wheat, to test the pathogenicity on the different host. The results showed more low pathogenicity on the wheat than triticale. The results of analysis of toxin type with 72 isolates, 64.6% isolates were produced nivalenol type toxin and other 4.6% and 30.8% isolates were produce 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol, respectively. To select fungicide for control, the 72 Fusarium isolates were cultivated on the media that containing four kinds fungicide. The captan, hexaconazole, and difenoconazole·propiconazole treated Fusarium isolates were not showed resistance response against each fungicide. However, six isolates out of 72 isolates, showed resistance response to fludioxonil. This study is first report that F. asiaticum causes Fusarium head blight disease of triticale in Korea.

• Korean Journal of Weed Science
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• v.31 no.2
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• pp.119-133
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• 2011

Sulfonylurea (SU)-resistant weeds include seven annual weeds such as Monochoria vaginalis, Scirpus juncoides and Cyperus difformis, etc., and three perennial weeds of Scirpus planiculmis, Sagittaria pigmaea and Eleocharis acicularis as of 2010 since identification Monochoria korsakowii in the reclaimed rice field in 1998. The Echinochloa oryzoides resistant to acetyl CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors has been confirmed in wet-direct seeding rice field of the southern province, Korea in 2009. In the beginning of occurrence of SU-resistant weeds the M. vaginalis, S. juncoides and C. difformis were rapidly and individually spreaded in different fields, however, theses resistant weeds have been occurring simultaneously in the same filed as time goes by. The resistant biotype by weed species demonstrated about 10- to 1,000-fold resistance, base on $GR_{50}$ (50% growth reduction) values of the SU herbicides tested. And the resistant biotype of E. oryzoides to cyhalofop-butyl, pyriminobac-methyl, and penoxsulam was about 14, 8, and 11 times more resistant than the susceptible biotype base on $GR_{50}$ values. In history of paddy herbicides in Korea, the introduction of SU herbicides including besulfuron-metyl and pyrazosulfuron-ethyl that control many troublesome weeds at low use rates and provide excellent crop safety gave farmers and many workers for herbicide business refreshing jolt. The products and applied area of SU-included herbicides have been rapidly increased, and have accounted for about 69% and 96%, respectively, in Korea. The top ten herbicides by applied area were composed of all SU-included herbicides by 2003. The concentrated and successive treatment of ACCase and ALS inhibitors for control of barnyardgrass in direct-seeded rice led up to the resistance of E. oryzoides. Also, SU-herbicides like pyrazosulfuron-ethyl and imazosulfuron which are effective to barnyardgrass can be bound up with the resistance of E. oryzoides. The ALS activity isolated from the resistant biotype of M. korsakowii to SU-herbicides tested was less sensitive than that of susceptible biotype. The concentration of herbicide required for 50% inhibition of ALS activity ($I_{50}$) of the SU-resistant M. korsakowii was 14- to 76-fold higher as compared to the susceptible biotype. No differences were observed in the rates of [$^{14}C$]bensulfuron uptake and translocation. ALS genes from M. vaginalis resistant and susceptible biotypes against SU-herbicides revealed a single amino acid substitution of proline (CCT), at 197th position based on the M. korsakowii ALS sequence numbering, to serin (TCT) in conserved domain A of the gene. Carfentrazone-ethyl and pyrazolate were used mainly to control SU-resistant M. vaginalis by 2006, the early period, in Korea. However, the alternative herbicides such as benzobicyclone, to be possible to control simultaneously the several resistant weeds, have been developing and using broadly because the several resistant weeds have been occurring simultaneously in the same filed. The top ten herbicides by applied area in Korea have been occupied by products of 3-way mixture type including herbicides with alternative mode of action for the herbicide resistant weeds. Mefenacet, fentrazamide and cafenstrole had excellent controlling effects on the ACCase and ALS inhibitors resistant when they were applied within 2 leaf stage.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.3
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• pp.202-212
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• 1992

This study reports on the genesis and mineralogical characteristics of the clay minerals in the soils derived from the five major parent rocks of granite, granite-gneiss, limestone, shale, and basalt in Korea. The investigation on the mineralogical aspects of primary and secondary minerals of the rocks and coarse fractions in the soils have been already reported. In this report, the identification of clay minerals in the soil clay fractions was done through the analyses of chemical, X-ray diffraction, and thermal methods. The studies showed clearly that much of the clay minerals was evolved by the weathering of primary minerals and some were further developed by the transformation of secondary minerals. Cation exchange capacity(CEC) of the clay fractions increased with higher amotunts of vermiculite, chlorite, and illite, however, decreased with higher hydroxy octahedral sheet within the interlayer spaces of vermiculite even if dominant clay with vermiculite. Feldspars in the granite and granite-gneiss might be completely transformed to kaolin mineral, Illite, chlolrite, and vermiculite formed by the alteration of micas, amphibole, augite, and primary chlorile seem to be subsequently transformed to the mixed layer minerals such as illite/vermiculite, illite/chlorite, and chlorite/vermiculite. These weathering products may be ultimately transformed into kaolin minerals. The smectite minerals in the clay fractions of the soils developed on the limestone are considerably present and they seem to be formed directly by the precipitation from high Mg solution and/or by the transformation of vermiculite from micas and chlorite in the parent materials. Abundant presence of illite in the soil clays developed on the shale is considered to have inherited from the fine particles and more resistant hydrous muscovite. The weathering sequences of the hydrous muscovite were as follows according to the degree of soil development ; hydrous muscovite ${\rightarrow}$ illite/vermiculite mixed layer(Inceptisols, Daegu series) and hydrous muscovite ${\rightarrow}$ illite/vermiculite mixed layer ${\rightarrow}$ vermiculite ${\rightarrow}$ kaolin mineral(Alfisols, Buyeo series). The plagioclase in the basalt might be mostly weathered to kaolin minerais. The augite in the basalt is likely to be transformed through progressive stage of weathering, augite ${\rightarrow}$ chlorite ${\rightarrow}$ chlorote/vermiculite mixed layer ${\rightarrow}$ vermiculite ${\rightarrow}$ kaolin. Another weathering sequence of augite could be expected, augite ${\rightarrow}$ chlorite ${\rightarrow}$ illite by the presence of illite and illite/vermiculite mixed layer in the clay fractions. Vermiculite and gibbsite were quantified from thermogravimetry(TG) and kaolin minerals, from both TG and differerential thermal analysis (DTA). Vermiculite in Jangseong series from the limestone was the dominant clay mineral of 21.7 percent and had a range in the order of 9.2 percent in Buyeo series to 5.4 percent in Daegu series from the shale. The rest soils ranged from 8.8 to 28.3 percent. Kaolin minerals were the dominant clay mineral of 32.7 percent in Asan series from the granite-gneiss and Gueom series of 32.0 percent from the basalt. The soils from the limestone ranged from 9.4 to 14.9 percent. The rest soils ranged from 8.9 to 28.6 percent. Gibbsite were 3.9 and 2.3 percent for Weoljeong and Chahang series from the granite, respectively. In Asan and Cheongsan series from the giranite-gneiss were 1.4 and 4.5 percent, respectively, and 3.6 percent in Jangpa series from the basalt.