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http://dx.doi.org/10.7847/jfp.2020.33.2.111

Genetic Identification and Biochemical Characteristics of Edwardsiella Strains Isolated from Freshwater Fishes Cultured in Korea  

Jang, Mun Hee (Inland Aquaculture Research Center, National Institute of Fisheries Science)
Kim, Keun-Yong (Department of Genetic Analysis, AquaGenTech Co., Ltd)
Lee, Yu Hee (Inland Aquaculture Research Center, National Institute of Fisheries Science)
Oh, Yun Kyung (Inland Aquaculture Research Center, National Institute of Fisheries Science)
Lee, Jeong-Ho (Inland Aquaculture Research Center, National Institute of Fisheries Science)
Song, Jun-Young (Inland Aquaculture Research Center, National Institute of Fisheries Science)
Publication Information
Journal of fish pathology / v.33, no.2, 2020 , pp. 111-118 More about this Journal
Abstract
The genus Edwardsiella belonging to the family Enterobacteriaceae is a member of Gram-negative rod-shaped bacteria that cause disease in diverse aquatic organisms such as fish, amphibians and reptiles as well as avians and mammals including human throughout the world. This genus had been composed of three species, E. hoshinae, E. ictaluri and E. tarda, but recent researches erected two novel species, E. anguillarum and E. piscicida that were conventionally identified as E. tarda. In this study, we isolated seven strains belonging to the genus Edwardsiella from freshwater fishes that had been reared at inland fish farms in South Korea and investigated their biochemical characteristics and molecular phylogenetic relationships. The seven strains showed typical characteristics of four Edwardsiella species, E. anguillarum, E. ictaluri, E. piscicida and E. tarda, by biochemical analyses of Gram staining, indole and hydrogen sulfide (H2S) production, and API (Analytic Profile Index) 20E test. Molecular phylogenetic analyses inferred from DNA sequence data of both 16S ribosomal RNA (rRNA) and DNA gyrase subunit B (gyrB) genes were congruent with the biochemical characteristics. As a result, both biochemical and molecular phylogenetic analyses identified four strains isolated from three Anguilla species as E. anguillarum, E. piscicida and E. tarda, two strains from Pelteobagrus fulvidraco and Silurus asotus as E. ictaluri, and one strain from Moroco oxycephalus as E. piscicida. In this study, we isolated and successfully identified recently newly erected species, E. anguillarum and E. piscicida in addition to historically notorious pathogenic species, E. ictaluri and E. tarda. In the future study, systematic and comprehensive monitoring of the four Edwardsiella species are required for studying differences in pathogenicity among freshwater fishes.
Keywords
Edwardsiella; E. tarda; E. piscicida; E. anguillarum; E. ictaluri; freshwater fish;
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