• Animal Systematics, Evolution and Diversity
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• v.26 no.3
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• pp.197-201
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• 2010

We sequenced the whole mitochondrial genome of Dendronephthya gigantea (Anthozoa: Octocorallia: Nephteidae), the first mitochondrial genome sequence report in the Family Nephtheidae. The mitochondrial genome of D. gigantea was 18,842 bp in length, and contained 14 protein coding genes (atp6 and 8, cox1-3, cytb, nd1-6 and 4L, and msh1), two ribosomal RNAs, and only one transfer RNA. The gene content and gene order is identical to other octocorals sequenced to date. The portion of the noncoding regions is slightly larger than the other octocorals (5.08% compared to average 3.98%). We expect that the information of gene content, gene order, codon usage, noncoding region and protein coding gene sequence could be used in the further analysis of anthozoan phylogeny.

• Journal of Plant Biotechnology
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• v.4 no.2
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• pp.45-52
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• 2002

A lot of SSRs (simple sequence repeats) in peach and pear from enriched genomic libraries and in peach from a cDNA library were developed. These SSRs were applied to other related species, giving phenograms of 52 Prunes and 60 pear accessions. Apple SSRs could also be successfully used in Pyrus spp. Thirteen morphological traits were characterized on the basis of the linkage map obtained from an $F_2$ population of peach. This map was compiled with those morphological markers and 83 DNA markers, including SSR markers used as anchor loci, to compare different peach maps. Molecular markers tightly linked to new root-knot nematode resistance genes were also found. A linkage map including disease related genes, pear scab resistance and black spot susceptibility, in the Japanese pear Kinchaku were constructed using 118 RAPD markers. Another linkage map, of the European pear Bartlett, was also constructed with 226 markers, including 49 SSRs from pear, apple, peach and cherry. Maps of other Japanese pear cultivars, i.e., Kousui and Housui, were also constructed. These maps were the first results of pear species.

• Journal of Life Science
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• v.8 no.4
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• pp.360-365
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• 1998

The genetic of a recently described virus, hepatitis G virus(HGV) was investigated. HGV envelope 1 (E1) nucleotide sequences isolated from six Korean hepatitis b virus-positive patients by using a reverse transcription-poly-merase chain reaction procedure, were analysed and compared to the seven previously reported HGV isolates. Sequence homology among the Korean isolates was 88-97% whereas among the isolates from different geographic areas was 80-92%, indicating geographical divergence of HGV. Nucleotide substitutions spread uniformly throughiut the E1 fragment. Furthermore, compared to the prototype HGV sequence, frameshift mutations were observed in most of the Korean isolating that a different translating initiation site for the polyprotein exists in the Korean type HGV.

• Journal of Communications and Networks
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• v.14 no.2
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• pp.206-212
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• 2012

In this paper, in order to effectively apply unitary space-time modulation to the direct-sequence spread-spectrum multiple-access (DSSS-MA) networks, we propose a low-rate, noncoherent, unitary, and space-time modulated DSSS system supporting any number of transmit antennas based on Walsh matrices. The proposed scheme simultaneously performs bandwidth spreading and space-time coding and outperforms those using high-rate, conventional unitary space-time constellations. Furthermore, the proposed scheme allows for a simple detector structure based on fast Walsh transforms.

• Animal Systematics, Evolution and Diversity
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• v.34 no.4
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• pp.215-219
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• 2018

Protochitonidae Ashby, 1925 is a family of small to medium sized chitons that includes a single fossil genus and two extant genera. Of the two extant genera, Deshayesiella Carpenter in Dall, 1879 contains 5 described species. Although most Deshayesiella species are known to be found in deep sea habitats(over 100 m), D. curvata (Carpenter in Pilsbry, 1892) is found from shallow waters(1-20 m). In this study, we provide details of microstructure of shell and radula characters using scanning electron microscopy and morphological features of D. curvata, and its partial sequence of mitochondrial DNA cox1 gene as DNA barcode sequence. In addition, we compare morphological differences of D. curvata from other congeneric species.

• Animal Systematics, Evolution and Diversity
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• v.35 no.1
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• pp.10-14
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• 2019

Acanthochitonidae Pilsbry, 1893 is one of the chiton families characterized by 9 pairs of sutural tufts on a well-expanded girdle that overgrows plates. It is found in intertidal and subtidal zones worldwide. Of its eight genera, only two have been previously reported in South Korea: Acanthochitona Gray, 1821 and Notoplax H. Adams, 1862. Here we report Leptoplax doederleini (Thiele, 1909) as the first record of the genus Leptoplax in South Korea and describe and compare its morphological features with N. kaasi Hong, Dell'Angelo and Van Belle, 1990. In addition, we provide the partial sequence of the mitochondrial DNA cytochrome c oxidase subunit I gene as a DNA barcode sequence record.

• Journal of the Korean Physical Society
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• v.73 no.12
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• pp.1908-1917
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• 2018

The binding affinity between the T-cell receptors (TCRs) and antigenic peptides mainly determines immunological recognition. It is not a trivial task that T cells identify the digital sequences of peptide amino acids by simply relying on the integrated binding affinity between TCRs and antigenic peptides. To address this problem, we examine whether the affinity-based discrimination of peptide sequences is learnable and generalizable by artificial neural networks (ANNs) that process the digital experimental amino acid sequence information of receptors and peptides. A pair of TCR and peptide sequences correspond to the input for ANNs, while the success or failure of the immunological recognition correspond to the output. The output is obtained by both theoretical model and experimental data. In either case, we confirmed that ANNs could learn the immunological recognition. We also found that a homogenized encoding of amino acid sequence was more effective for the supervised learning task.

• Animal Systematics, Evolution and Diversity
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• v.39 no.3
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• pp.118-122
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• 2023

The genus Hydroporus Clairville is the most diverse group belonging to the subfamily Hydroporinae in the family Dytiscidae. However, members of this group have not been recorded in the Korean peninsula. The genus Hydroporus Clairville is characterized by the following features: body glabrous to distinctly setose; elytra and ventral surface mainly piceous to black; epipleuron lacking a carina on humeral angle; metacoxal process conjointly with hind margin straight or slightly angulate medially. We collected the high mountain wetland and identified the species of Hydroporus uenoi Nakane based on male genitalia for the first time. A habitus photo, a redescription, mitochondrial DNA sequence, illustrations of the aedeagus of species, and habitat information are provided.

• Journal of Ginseng Research
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• v.44 no.4
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• pp.637-643
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• 2020

Background: Ginseng (Panax ginseng Meyer) is one of the world's most valuable medicinal plants with numerous pharmacological effects. Ginseng has been cultivated from wild mountain ginseng collections for a few hundred years. However, the genetic diversity of cultivated and wild ginseng populations is not fully understood. Methods: We developed 92 polymorphic microsatellite markers based on whole-genome sequence data. We selected five markers that represent clear allele diversity for each of their corresponding loci to elucidate genetic diversity. These markers were applied to 147 individual plants, including cultivars, breeding lines, and wild populations in Korea and neighboring countries. Results: Most of the 92 markers displayed multiple-band patterns, resulting from genome duplication, which causes confusion in interpretation of their target locus. The five high-resolution markers revealed 3 to 8 alleles from each single locus. The proportion of heterozygosity (H_e) ranged from 0.027 to 0.190, with an average of 0.132, which is notably lower than that of previous studies. Polymorphism information content of the markers ranged from 0.199 to 0.701, with an average of 0.454. There was no statistically significant difference in genetic diversity between cultivated and wild ginseng groups, and they showed intermingled positioning in the phylogenetic relationship. Conclusion: Ginseng has a relatively high level of genetic diversity, and cultivated and wild groups have similar levels of genetic diversity. Collectively, our data demonstrate that current breeding populations have abundant genetic diversity for breeding of elite ginseng cultivars.

• Environmental Engineering Research
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• v.12 no.5
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• pp.231-237
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• 2007

The microbial eco-physiology has been the vital key of microbial ecological research. Unfortunately, available methods for direct identity of microorganisms and for the investigation of their activity in complicated community dynamics are limited. In this study, metagenomics was considered as a promising functional genomics tool for improving our understanding of microbial eco-physiology. Its potential applications and challenges were also reviewed. Because of tremendous diversity in microbial populations in environment, sequence analysis for whole metagenomic libraries from environmental samples seems to be unrealistic to most of environmental engineering researchers. When a target function is of interest, however, sequence analysis for whole metagenomic libraries would not be necessary. For this case, nucleic acids of active populations of interest can be selectively gained using another cutting-edge functional genomic tool, SIP (stable isotope probing) technique. If functional genomes isolated by SIP can be transferred into metagenomic library, sequence analysis for such selected functional genomes would be feasible because the reduced size of clone library may become adequate for sequencing analysis. Herein, integration of metagenomics with SIP was suggested as a novel functional genomics approach to study microbial eco-physiology in environment.