• KSBB Journal
• /
• v.23 no.6
• /
• pp.557-560
• /
• 2008

The micelle process was developed for pre-purifying paclitaxel from plant cell cultures of Taxus chinensis, giving a high purity and yield. The approach in this work was to transfer paclitaxel in the crude extract to an aqueous surfactant solution as a micelle, allowing organic solvents to be used for removal of lipids and non-polar impurities. In this work, the effects of various surfactants such as CPC, CTMAC, LTMAC, SDS, AOT, Tween, PEG, and Triton were examined on the yield, purity, and phase separation time in micelle process. Among these surfactants, CTMAC (5%, w/v) gave the best result in terms of paclitaxel yield (${\sim}99%$), purity (${\sim}21%$), and phase separation time (30 min). The use of micelles in the pre-purification process allows for rapid and efficient separation of paclitaxel from interfering compounds and dramatically increases the yield and purity of crude paclitaxel for subsequent purification steps.

• Journal of the Korean GEO-environmental Society
• /
• v.13 no.12
• /
• pp.59-66
• /
• 2012

In this study, it was sampling from heavy metal-contaminated soil with the waste in railroad workshop. And, the pollution concentration and analysis of particle-size distribution were conducted to design efficient purification process that it was aimed at high contaminated area, low contaminated area and samples containing waste foundry sand. But, it was the other signs of general soil contamination, as construction waste of waste concrete and waste wood, waste foundry sand, incinerator ash, etc is overall buried on the grounds. Thus, the common heavy metal purification technology has not decreased the pollution. However, heavy-metal contamination was reduced by magnetic separation utilizing the magnetic component of the mixed waste.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2010.06a
• /
• pp.44-44
• /
• 2010

Conventional water treatment facilities like precipitation process need large-scale equipment and wide space to purify the wastewater of paper factory. In case of massive waste water, high gradient magnetic separation (HGMS) parts are more effective to purify it rapidly and to occupy relatively small space, since large voids at filter with HGMS are adopted. Cryo-cooled Nb-Ti superconducting magnet with room temperature bore in diameter of 100 mm and 600 mm in height was used for magnetic separator. Magnetic filters were designed by the analysis of magnetic field distribution at superconducting magnets.

• Journal of Ginseng Research
• /
• v.12 no.2
• /
• pp.164-172
• /
• 1988

The solvent separation step in the conventional method for quantitative analysis of ginsenosides was substituted by purification through a small reverse-phase $C_18$-column resulting in the decrease of analysis time by one fourth. New method showed high recovery of total ginsenosides but low recovery in protopanaxatriol-ginsenosides. Sugars did not affect the recovery by the amount in usual root sample. Coefficient of variation in recovery of ginsenosides was lower in the rapid minicolumn method. Optimum load of ginsenosides to minicolumn was 10 to 15 mg. The rapid minicolumn method showed highly significant correlation with the solvent separation method for dried root and red ginseng. For the rapid minicolumn method a small acryl device was used for the simultaneous extraction of 8 samples. This method appeared to be beneficial in cost and for the health of analyst.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.2 no.2
• /
• pp.113-116
• /
• 1997

The hydrogel process is a different form of ultrafiltration and has been used to separate biological molecules. In this study, the gel pore size was predicted by pulse NMR technique and neural network using a database obtained from gel filtration chromatography and diffusion experiment. Recombinant alkaline phosphatase expressed in insect cells was concentratred 1.5 times by hydrogel ultrafiltration by swelling at 2$0^{\circ}C$ and collapsing at 35$^{\circ}C$ at 53-65% separation efficiency and 78-83% enzyme recovery. Wild and recombinant Autographa californica unclear polyhedrosis viruses (AcNPV) were also concentrated 1.4 and 1.6 times of the feed solution at 48.5 and 60.0% separation efficiency, respectively Hydrogel ultrafiltration appears to be an attractive alternative for the concentration of AcNPV and recombinant proteins from insect cells.

• Journal of the Korean Society of Industry Convergence
• /
• v.27 no.3
• /
• pp.601-607
• /
• 2024

The discharge of oily wastewater into water bodies and soil poses a serious hazard to the environment and public health. Various conventional techniques have been employed to treat oil-water mixtures and emulsions; Unfortunately, these approaches are frequently expensive, time-consuming, and unsatisfactory outcomes. Porous materials and adsorbents are commonly used for purification, but their use is limited by low separation efficiencies and the risk of secondary contamination. Recent advancements in nanotechnology have driven the development of innovative materials and technologies for oil-contaminated wastewater treatment. Nanomaterials can offer enhanced oil-water separation properties due to their high surface area and tunable surface chemistry. The fabrication of nanofiber membranes with precise pore sizes and surface properties can further improve separation efficiency. Notably, novel technologies have emerged utilizing nanomaterials with special surface wetting properties, such as superhydrophobicity, to selectively separate oil from oil-water mixtures or emulsions. These special wetting surfaces are promising for high-efficiency oil separation in emulsions and allow the use of materials with relatively large pores, enhancing throughput and separation efficiency. In this study, we introduce a facile and scalable method for fabrication of superhydrophobic-superoleophilic felt fabrics for oil/water mixture and emulsion separation. AlN nanopowders are hydrolyzed to create the desired microstructures, which firmly adhere to the fabric surface without the need for a binder resin, enabling specialized wetting properties. This approach is applicable regardless of the material's size and shape, enabling efficient separation of oil and water from oil-water mixtures and emulsions. The oil-water separation materials proposed in this study exhibit low cost, high scalability, and efficiency, demonstrating their potential for broad industrial applications.

• Journal of Radiopharmaceuticals and Molecular Probes
• /
• v.5 no.2
• /
• pp.71-78
• /
• 2019

Nuclear imaging is one of the most powerful measures for non-invasive diagnosis of myocardial vascular disease. Radionuclide such as ¹³N, ¹⁵O, ²⁰¹Tl and ⁸²Rb is used for the measurement of cardiac blood flow. ¹³N, ¹⁵O and ²⁰¹Tl are produced in cyclotrons while ⁸²Rb is obtained from generator. Rubidium (Rb), an alkali ion, behaves biologically like potassium, and accumulates in myocardial tissue. Rb has rapid blood clearance profile which allows the use of ⁸²Rb with a short physical half-life of 75 s for non-invasive evaluation of regional myocardial perfusion. There are several advantages of ⁸²Rb over other radioisotopes. An ultra-short half-life significantly reduces the exposure of patients to radiation and allows to repeat injections for studying the effects of medical intervention. As a positron emitter, ⁸²Rb allows positron emission tomography (PET) imaging which have shown superior diagnostic performances. ⁸²Rb can be produced from generator by decay of its parent ⁸²Sr. However, the preparation of ⁸²Sr is difficult, because appropriate purity is required to meet the specification of the product. Recently reported procedure from ARRONAX research institute showed that a Chelex-100 resin is sufficient for this purpose and additional column is not necessary. Whereas Brookhaven National Laboratory (BNL) procedure contains three ion exchange resin separation, including Chelex-100 resin. Currently, since ⁸²Sr production site is non-existent in Korea, Korea Atomic Energy Research Institute (KAERI) has plan to produce ⁸²Sr within specifications. We compared ⁸²Sr purification procedures reported from ARRONAX and BNL to investigate the most suitable procedure for our conditions.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.1
• /
• pp.1-8
• /
• 2016

The new movement towards green chemistry and renewable feedstocks makes microbial production of chemicals more competitive. Among the numerous chemicals, organic acids are more attractive targets for process development efforts in the renewable-based biorefinery industry. However, most of the production costs in microbial processes are higher than that in chemical processes, among which over 60% are generated by separation processes. Therefore, the research of separation and purification processes is important for a promising biorefinery industry. This review highlights the progress of recovery processes in the separation and purification of organic acids, including their advantages and disadvantages, current situation, and future prospects in terms of recovery yields and industrial application.

• Membrane Journal
• /
• v.15 no.2
• /
• pp.121-131
• /
• 2005

Separation and purification of lysozyme from chicken egg white was studied using ultrafiltration. We have obtained experimental data through the cellulose membranes with the molecular weight cut off (MWCO) of 10 kDa, 30 kDa and 100 kDa in a stirred ultrafiltration device. Certain amounts of egg white were dissolved into 20 mM phosphate buffers of pH 6, 7 and 8 to make protein solutions of $1\%,\;2\%,\;3\%\;and\;10\%$ concentration. Permeation flux increased with increasing MWCO of the membrane. Permeation flux increased with increasing transmembrane pressure (TMP) and decreasing the protein concentration. As the MWCO of membrane decreased, the selectivity increased. The selectivity increased with increasing TMP and protein concentration of the solution.

• Clean Technology
• /
• v.20 no.4
• /
• pp.406-414
• /
• 2014

Biogas is a gaseous mixture produced from microbial digestion of organic materials in the absence of oxygen. Raw biogas, depending upon organic materials, digestion time and process conditions, contains about 45-75% methane, 30-50% carbon dioxide, 0.3% of hydrogen sulfide gas and fraction of water vapor. To achieve the standard composition of the biogas the treatment techniques like absorption or membrane separation was performed for the resourcing of biogas. In this paper the experimental results of the methane purification in simulated biogas mixture consisted of methane, carbon dioxide and hydrogen sulfide were presented. The composite membrane is manufactured within polysulfone in order to increase the separation performances for the gaseous mixtures of $CO_2$ and $CH_4$ which are main components of the biogas. The effects of feed pressures and mixed gas on the separation of $CO_2-CH_4$ by membrane are investigated. Chelate chemical was utilized to treat the purification of methane from the $H_2S$ concentration of 0.3%.