• 한국동물생명공학회지
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• 제35권2호
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• pp.142-148
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• 2020

The research work was undertaken to determine an effective fertilization medium, sperm separation method and sperm capacitating agent for optimum in vitro fertilization (IVF) rates of indigenous zebu cow oocytes. In experiment 1, tissue culture medium (TCM 199), Tyrode's albumin lactate pyruvate (TALP) and Brackett and Oliphant (BO) medium were used as basic medium for IVF of oocytes of indigenous zebu cows. In experiment 2, three sperm separation methods namely centrifugation, swim up and percoll gradient methods were used for separation of motile and viable spermatozoa for IVF. In experiment 3, for capacitation of spermatozoa, IVF medium supplemented with the heparin, mixture of penicillamine, hypotaurine and epinephrine (PHE) or the combination of heparin with PHE were used for fertilization. In vitro culture (IVC) of presumptive zygotes was done in modified synthetic oviduct fluid (mSOF) medium using standard procedure 24 h after sperm-oocytes co-culture. The cleavage rate was determined to evaluate the efficacy of fertilization medium, sperm separation method and sperm capacitating agent 24 h after IVC. The cleavage rate was higher in oocytes fertilized in TALP (63.3%) than in TCM 199 (47.5%) (p < 0.05). The cleavage rate was higher in oocytes fertilized by spermatozoa separated by percoll gradient method (62.3%) than by centrifugation (51.6%) (p < 0.05). The cleavage rate of oocytes was higher when insemination was done with spermatozoa capacitated in TALP supplemented with heparin and PHE (61.3%) compared to control (40.9%) (p < 0.05). In conclusions, TALP based medium and percoll gradient sperm separation followed by capacitation with combination of heparin and PHE are suitable for IVF of indigenous zebu cow oocytes in Bangladesh.

• 한국미생물·생명공학회지
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• 제27권5호
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• pp.378-384
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• 1999

Protopectinase (PPases) are heterologous group of enzymes that degrade pectin from the insoluble protopection which is constituent of the middle lamella and primary cell wall of higher plants by restricted depolymerization. From the previous report[6], enzymatically separated plant cells, which are produced from plant tissues by PPases treatment, showed well-conserved cellular components with their rigid cell wall and this characteristic is applicable to preparation of novel food material. The purpose of this study is to investigate the effect of medium composition of PPase production from Bacillus subtilis EK11 which was selected as a PPase producer. Various carbon sources and concentrations on PPase production were studied and corn starch at 0.7% was the most effective for production of PPase. Among the nitrogen sources, yeast extract was the most effective for PPase production and the effect of (NH4)2SO4 was notable as inotganic nitrogen source. Inorganic compounds such as KH2PO4, K2HPO4, Na3-citrate.2H2O and MgSO4 were optimized for PPase production. PPase activity was inhibited by the adition of Ba2+ or Zn2+. The optimal medium for PPase production was devised: 0.7% corn starch, 0.3% yeast extract, 1.4% KH2PO4, 0.6% K2HPO4, 0.1% Na3-citrate.2H2O and 0.02% MgSO4. PPase production by using the optimum medium was carried out with shaking cultivation at 37$^{\circ}C$. The maximum PPase activity of 256unit/ml could be obtained after the cultivation for 48hrs. The activity was increased about 2.2timesthan the activity, 112 unit/ml, in basal medium.

• 대한기계학회논문집B
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• 제41권9호
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• pp.571-577
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• 2017

본 연구는 수치해석 기법을 활용하여 오일샌드 플랜트에 사용되는 다상유동분리기의 다양한 유입구 형상에 따른 효율 분석에 관한 연구이다. 본 연구에 사용된 유수분리기(FWKO, Free-water knockout)는 유량 $15,89m^3/d$(100 bbl/d), SOR(Steam-to-Oil Ratio) 3.5의 값을 가지며 Stokes 이론을 기반으로 설계되었다. 모듈화를 위하여 두 개의 유수분리기를 병렬 연결하였고 이에 따른 유입구 형상 최적화를 수행하였다. 유입구를 통해 유입되는 비투멘 에멀젼은 $150^{\circ}C$, 50 bar이며, API는 17의 값을 갖는다. 유수분리공정의 평균체류시간은 물과 오일이 95% 분리되는 시간으로 정의하였다. 다상유동의 밀도차에 의한 중력분리과정을 모사하기 위하여 유한체적법(VOF, Volume Of Fluid)과 상차분모델(DPM, Discrete Phase Model)을 조합하여 활용하였으며 준과도(Pseudo-transient) 해석기법을 활용하였다.

• 대한조선학회논문집
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• 제29권2호
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• pp.115-122
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• 1992

Sandwich구조는 복합구조의 특별한 hybrid구조의 형태로써 두 층의 얇은 표면재와 가운데 두꺼운 층인 경량의 심재로 구성되어 있으며, 이는 표면재와 심재가 접착된 일체로써 서로의 단점을 보완하면서 구조 효율을 높인 형상이다. 또한 심재는 표면재에 비해서 상당히 두꺼운 두께를 갖기 때문에 전단 변형이 중요하므로 굽힘 강성도(bending stiffness) 계산에 전단효과가 고려되어야 한다. 구조 설계에서 중요한 목적은 중량 감소에 있기 때문에 본 논문에서는 sandwich panel의 설계시 표면재와 심재의 두께 및 재질을 변화시켜 제한조건에 맞는 최소 중량을 얻는 데 중점을 두었다. 본 해석에서 sandwich panel의 최소중량을 얻기 위하여 표면재와 전단력을 고려한 심재의 변형에 따른 변형에너지를 각각 계산한 후, 최소 potential 에너지 원리를 적용하여 목적함수의 최적치를 구하였다. 설계 제한조건으로는 허용 처짐, 허용 굽힘응력, 허용 전단응력과 국부적인 불안정 상태의 wrinkling 응력이 고려되었으며, 설계 모델은 수직 분포 하중에 의한 여러가지 경계조건에 따른 sandwich panel을 대상으로 하였다. 비선형 최적화 기법은 Nelder and Mead Simplex Search Method와 Hooke and Jeeves Pattern Search method에 External Penalty Function이 적용된 SUMT방법을 결합시킨 SUMTNM와 SUMTHJ를 사용하였으며, 해석 결과는 sandwich panel의 구조 설계에 활용할 수 있도록 표를 작성하였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.631-631
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• 2013

Battery has major drawbacks including its size and life expectancy, and environmental problem. As an alternative, energy harvesting is emerging as a potential solution to replace battery along with more energy-efficient IT devices. The idea of harnessing energy from our living environment is sustainable, semi-permanent, and eco-friendly. Also, unlike battery, energy harvester does not require much space to store energy. Therefore, energy harvesting can provide a better source of power for small, portable, and wireless devices. Among various ways of harvesting energy from our surroundings, triboelectricity is chosen due to its potential to be miniaturized, and efficient. Triboelectric effect occurs as two different materials with different polarity of charge separation come into contact through friction, and then become separated so that electric potential difference is achieved. In this research, such characteristic of triboelectricity is used as a way to convert ambient mechanical energy into electric energy.Series of recent researches have shown promising results that the triboelectric energy harvester can be simple and cost effective. However, sufficient electricity level required to operate mobile devices has not yet been achieved.In this research, our group focuses on the design and optimization of triboelectric energy harvesting device to enhance its output. By using maskless lithography to pattern Kapton film and silicon substrate, which is used as a mold for PDMS thin layer, and sputtering metal electrodes on each side, we fabricate and demonstrate different designs of triboelectric energy harvester that utilizes the contact electrification between a polymer thin film and a metal thin foil. In order to achieve optimized result, the output voltage and current are measured under diverse conditions, which include different surface structure and pattern, material, and the gap between layers.

• 한국식품과학회지
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• 제28권2호
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• pp.378-383
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• 1996

감귤과피로부터 다양한 생리활성 효과와 기능적 특성을 갖는 식이섬유(dietary fibers, DF)를 생산하기 위한 회분식 다단계 한외여과 공정에서 최적 분리 조건은 막횡단압력 7.5 psi,막분리 온도 $35^{\circ}C$, 과피추출즙 pH 3.0으로 결정하였으며, MWCO 단계별 flux 측정에서는 YM10이 가장 높은 값을 나타내었으나 retentate 회수율과 DF 함량면에 있어서는 YM100 단계가 더 효율적인 것으로 나타났다. 각 단계별 총식이섬유 (total dietary fiber, TDF) 함량 비교 결과 본래 과피의 TDF 함량은 33.4%, 170 mesh retentate의 TDF 함량은 18.5%, YM100 retentate에서의 TDF 함량은 8.4%로 나타나 거의 대부분의 DF가 예비여과와 YM100단계에서 회수됨을 알 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제31권10호
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• pp.2813-2818
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• 2010

A novel method was developed for the speciation of chromium in natural water samples based on homogeneous liquid-liquid extraction and determination by flame atomic absorption spectrometry (FAAS). In this method, Cr(III) reacts with a new Schiff's base ligand to form the hydrophobic complex, which is subsequently entrapped in the sediment phase, whereas Cr(VI) remained in aqueous phase. The Cr(VI) assay is based on its reduction to Cr(III) by the addition of sodium sulfite to the sample solution. Thus, separation of Cr(III) and Cr(VI) could be realized. Homogeneous liquid-liquid extraction based on the pH-independent phase-separation process was investigated using a ternary solvent system (water-tetrabutylammonium ion ($TBA^+$)-chloroform) for the preconcentration of chromium. The phase separation phenomenon occurred by an ion-pair formation of TBA and perchlorate ion. Then sedimented phase was separated using a $100\;{\mu}L$ micro-syringe and diluted to 1.0 mL with ethanol. The sample was introduced into the flame by conventional aspiration. After the optimization of complexation and extraction conditions such as pH = 9.5, [ligand] = $1.0{\times}10^{-4}\;M$, [$TBA^+$] = $2.0{\times}10^{-2}\;M$, [$CHCl_3$] = $100.0\;{\mu}L$ and [$ClO_4$] = $2.0{\times}10{-2}\;M$, a preconcentration factor (Va/Vs) of 100 was obtained for only 10 mL of the sample. The relative standard deviation was 2.8% (n = 10). The limit of detection was sufficiently low and lie at ppb level. The proposed method was applied for the extraction and determination of chromium in natural water samples with satisfactory results.

• 대한토목학회논문집
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• 제34권6호
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• pp.1911-1918
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• 2014

건설공사 프로젝트에서 인력 및 장비 등의 투입자원 증가는 공정간의 작업공간 간섭을 유발할 뿐만 아니라 프로젝트의 생산성과 품질에 영향을 미칠 수 있다. 이를 해결하기 위해 작업공간관련 연구가 수행되고 있으나, 단순히 공정들의 기하학적 위치만을 고려하여 작업공간을 검증하거나, 작업공간의 형상을 세부 작업별 분리된 형상이 아닌 일괄적 하나의 객체 형태로 생성하고 있다. 이러한 방식은 공정의 특성과 자원의 투입 시간에 따라 변화하는 작업공간의 크기를 반영하지 못하므로 합리적인 작업공간 충돌의 분석이 어렵게 된다. 이를 위해 본 연구에서는 공정과 자원의 특성에 적합한 작업공간의 형상과 크기를 생성할 수 있도록 작업공간의 3차원 모델 생성방법과 운용 시스템을 개발한다. 또한 이를 적용한 작업공간 간섭최적화 연계방안과 건설장비의 최적 이동경로 탐색 방법론을 개발하여 실무적 활용성을 검증한다.

• 한국실내디자인학회논문집
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• 제21권6호
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• pp.79-91
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• 2012

In the Digital Convergence era, the center of social networking is moving into on-line space. This means also that space for public is moving into the on-line space. Along with this, spaces in commercial area, offered as attraction factors, are taking a role as a public space. So, this paper defines these spaces mentioned above as public space and the like. Liberal and affluent communication of Digital Convergence has caused in new spatial cognitions such like, constant social space, flowing space, temporary space, and multiple space. This means the hybridization of on-line and off-line space and the advent of public convergent space. However, it is on-line-centered convergence and has positive effects and negative effects on relationship. This paper suggests the optimization of public convergent space to solve the problems and make better a public space for relationship. For achieving this, social disclosure is grasped as the common way to start relationship both off-line and on-line, and it is proved that social disclosure has three characters such as self-presentation, corporeality, and subjectivity. Subsequently, the differences of the roles of off-line and on-line space are separated by each individual character. These are self-presentation of performance vs. storytelling, corporeality of embodiment vs. disembodiment, and self-subjectivity vs. inter-subjectivity. By recognizing that there are multilevel spectrums between the formers and the latters, this paper presents the direction of the spatial configuration of public convergent space which offers the right of manipulation of self-disclosure. It will be used for presenting the prototype of public convergent space.

• 한국자원공학회지
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• 제55권6호
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• pp.517-526
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• 2018

본 연구는 가학광산 오염토양 정화를 위해 토양 세척 최적조건을 수립하는데 목적이 있다. 연구지역 내 토양은 다양한 중금속에 의해 오염되어 있는데 특히 구리, 납, 아연의 농도가 높게 나타난다. 염산을 활용한 세척실험 결과 1 M의 농도로 30분 세척 시 중금속의 제거 효과가 가장 좋게 나타났다. 전체 토양의 38%는 1.18 mm 이상의 굵은 입자로 오염도가 상대적으로 낮으나 0.075 mm 이하의 작은 입자들은 오염도가 높아 고농도의 염산용액과 긴 세척시간에도 불구하고 오염도를 기준치 이내로 낮추기 어려웠다. 따라서 오염도가 높고, 중금속 농도 저감이 어려운 작은 토양입자들을 토양세척 이전에 물리적인 방법으로 분리할 필요가 있는 것으로 판단된다. 습식 사이클론을 활용한 연속식 토양세척 실험 결과 20% 이상의 미립자를 제거한 경우 오염토양의 중금속 농도를 기준치 이내로 저감시킬 수 있었다.