• The Journal of Korean Medicine
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• v.29 no.4
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• pp.55-67
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• 2008

Objectives: The purpose of this study was to investigate the effect of Hwangryunhaedok-tang, its major component Coptidis Rhizoma, and the major component of Coptidis Rhizoma, berberine, on the expression of behavioral sensitization induced by the repeated administration of nicotine. Methods: Experimental ratswere divided into 3 groups and each group treated with saline, nicotine, and Hwangryunhaedok-tang. Experiments were performed on 4 further groups, each group treated with saline, nicotine, Coptidis Rhizoma, and berberine. To obtain rats addicted todrugs, nicotine was injected repeatedly. After 3 days of withdrawal phase, on the 11th day, rats were re-exposed to nicotine and thus able to induce the expression of the activity sensitization phenomenon successfully. To examine the change of c-Fos in the brain of experimental rats with the nicotine-induced activity sensitization, their neurological activation rate was assessed by c-Fos staining with Fos-like immunihistochemistry of the dopamine releasing area, the striatum and the nucleus accumbens. Results: The result demonstrate that Hwangryunhaedok-tang, its major component Coptidis Rhizoma and berberine,the major component of Coptidis Rhizoma, significantly reduced the walking activity rate in the drug addict models induced by nicotine administration, and Coptidis Rhizoma and its major component berberine reduce the marker of the neurological activation c-Fos, and thus revealed an effect of suppressing drug addiction. Conclusions: These results suggest the possibility of Hwangryunhaedok-tang, its major component Coptidis Rhizoma and the major component of Coptidis Rhizoma, berberine,as therapeutic for nicotine addiction.

• Toxicological Research
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• v.18 no.2
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• pp.175-181
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• 2002

Allergic contact dermatitis (skin sensitization) may be caused by a wide variety of chemicals. A murine local lymph node assay (LLNA) has been developed as an alternative to guinea pig models for assessing the contact sensitization potential of chemical. This study was carried out to evaluate the skin sensitization potential for chemicals in Balb/c mice by LLNA. Contact allergen, dinitrochlorobenzene (DNCB), respiratory allergen, toluene diisocyanate (TDI) and a weak allergen, $\alpha$-hexlycinnamaldehyde (HCA) were wed as positive chemicals and irritant, sodium lauryl sulfate(SLS) also wed as a reference chemical in this study. The weights of lymph node in the mice treated with DNCB, TDI, and HCA were increased compared to vehicle control. There was a significant increase in lymph node weight of mice treated with high concentration of SLS compared to vehicle control. The stimulation index (SI) of Lymph node cell in the mice treated with DNCB, TDI, and HCA revealed over three-fold increase compared to vehicle control by $3^H$-thymidine uptake. All allergens correctly identified in this LLNA study wing Balb/c mice. These results suggest that LLNA wing Balb/c mice could be a useful method for screening the allergenic potential of chemicals. The expression of IL-2 mRNA was slightly increased in draining auricular lymph node cell of the mice treated with TDI and HCA by RT-PCR. However the IL-2 levels in DNCB and SLS of treated animals were not significantly changed.

• Clinical and Experimental Pediatrics
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• v.57 no.1
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• pp.29-34
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• 2014

Purpose: In this study, we aimed to investigate the prevalence of year-round respiratory viral infection in children with lower respiratory tract infection (LRTI) and the relationship between respiratory viral infection and allergen sensitization in exacerbating asthma. Methods: We investigated the sources for acute LRTIs in children admitted to our hospital from May 2010 to April 2011. The 6 most common respiratory viruses were isolated from nasopharyngeal aspirate using multiplex reverse transcription-polymerase chain reaction in 309 children; respiratory syncytial virus (RSV), adenovirus (AV), parainfluenza virus (PIV), influenza virus (IFV), human metapneumovirus (hMPV), rhinovirus (RV). Atopic sensitization was defined if more than 1 serum specific Immunoglobulin E level measured using UniCAP (Pharmacia) was over 0.35 IU/mL. Results: RSV was the most common pathogen of bronchiolitis in hospitalized children through the year. RV or IFV infection was more prevalent in asthma exacerbations compared to other LRTIs. AV and hMPV were more likely to cause pneumonia. RV and IFV were associated with asthma exacerbations in children with atopic sensitization, but not in nonatopic children. Conclusion: RV and IFV are associated with hospitalization for asthma exacerbation in children with atopic sensitization.

• Biomedical Science Letters
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• v.19 no.4
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• pp.315-329
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• 2013

This study was conducted to evaluate the distribution, frequency, sensitization rate, and types of specific allergen in allergic patients. We analyzed allergens of 12,882 allergic patient's serum referred for Medical Laboratory using MAST Allergy Screen Test (Inhalation/food panel) from April, 2010 to March, 2011. Allergen reactivity were not detected in 833/12,882 (6.5%) allergic patients and the other allergic patients had two or more types of allergens of food and inhalant, respectively. In the distribution of the allergen food 73.4% (9,450/12,882 patients) was much higher than the pollen 26.6% (3,432/12,882 patients). The sensitization rate to each allergen showed garlic 26.7%, egg white 21.5% of food allergens, mugwort 24.9%, ragweed short 19.6% of pollen allergens, Candida albicans 10.5%, Alternaria spp. 7.9% of fungus allergens, and showed high sensitized rate to Dermatophagoides farinae 90.2%, Dermatophagoides pteronyssinus 77.8%, house dust 57% of indoor allergens, respectively. The specific allergens of food allergen, fungus allergen, and indoor allergen were more frequent in young child than adult, but pollen allergens were frequent in adult than young child. In seasonal distribution, the food allergen were frequent in summer and autumn, the pollen allergen in autumn, fungus allergen in spring and in winter, and indoor allergen in autumn and in winter. In conclusion, the mite of Dermatophagoides farinae and Dermatophagoides pteronyssinus, and house dust showed the highest sensitization rate in specific allergen of allergic patients. The allergens showed difference feature according to the age, region, and types of allergen.

• Journal of Welding and Joining
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• v.31 no.5
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• pp.47-53
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• 2013

We have investigated the traits of clad metals in hot-rolled clad steel plates, including the sensitization and mechanical properties of STS 316 steel plate and carbon steel (A516), under various specific circumstances regarding post heat treatment, multilayered welds, and thick or repeated welds for repair. For evaluations, sectioned weldments and external surfaces were investigated to reveal the degree of sensitization by micro vickers hardness, tensile, and etching tests the results were compared with those of EPR tests. The clad steel plates were butt-welded using FCAW and SAW with the time of heat treatment as the variable, a that was conducted at $625^{\circ}C$, for 80, 160, 320, 640, and 1280 min. Then, the change in corrosion resistance was evaluated in these specimens. With carbon steel (A516), as the heat treatment time increased, the annealing effect caused the tensile strength to decrease. The micro-hardness gradually increased and decreased after 640 min. The elongation and contraction of the area also increased gradually. The oxalic acid etch test and EPR test on STS316 and the clad metal showed STEP structure and no sensitization. From the test results on multi-layered and repair welds, it could be concluded that there is no effect on the corrosion resistance of clad metals. The purpose of this study was to suggest some considerations for developing on-site techniques to evaluate the sensitization of stainless steels.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.22 no.2
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• pp.201-210
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• 1996

Predictive tests for the identification of contact sensitizing chemicals have been developed. We measured the sensitization potential with three predictive tests, the in vitro and the in vivo Local Lymph Node Assay(LLNA), ELISA to detect interferon-gamma(IFN-${\gamma}$) from supernatant and flow cytometry to detect change of cell surface proteins, using draining lymph nodes of mice. BALB/c mice were exposed to various chemicals or vehicles on the ears daily for 3 consecutive days in all experiments. With some exceptions of propyl paraben, neomycin sulfate, the in vivo LLNA was able to detect the sensitizing capacity of test chemicals and was more sensitive than the in vitro LLNA for chemicals used in the present study. In another experiment, contact sensitivity was assessed by the ELISA to detect IFN-Υ from the supernatants of the cultured LNCs after sensitization with chemicals. There was a good correlation between the LLNA and the IFN-Υ production for test chemicals. We also examined the change of cell surface proteins on LNCs after sensitization by flow cytometry for some cell adhesion molecules(ICAM-1, E-cadherine, B7 molecule), T cell markers(CD3, CD4, CD8, T$\alpha$$\beta$,T${\gamma}$$\delta$) and B cell markers(LR1, CD45R, I-Ad). The number of ICAM-1 positive cells and B cells in LNCs were increased after sensitization with DNCB, TNCB, isoeugenol and 25%, 50% cinnamic aldehyde compared with that of vehicle as a control. In conclusion, the in vivo LLNA could provide more sensitive screening test for moderate to strong sensitizers and some weak sensitizers including cosmetic raw materials than the in vitro LLNA. The production of IFN-Υ by allergen-activated LNCs might be a values indicators without radioisotopes for the identification of contact allergens. Detection of allergens by testing the increase of ICAM-1 positive cells and B cells in LNCs by flow cytometry might be used as a test method to detect allergens.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.24 no.5
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• pp.492-499
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• 2021

Purpose: Celiac disease (CD) is an autoimmune disorder of the small intestine caused by an abnormal immune response to gluten proteins and is often characterized by gastrointestinal symptoms. Food allergy (FA) is an adverse immune sensitivity to ingested food proteins leading to inflammation in various organs including the gastrointestinal tract. The relationship between CD and FA remains unclear. This study aimed to assess the prevalence and clinical relevance of immunoglobulin E (IgE)-mediated food sensitization in children with CD. Methods: Fifty-nine children diagnosed with CD were reviewed for clinical symptoms and evidence of IgE-sensitization to food and airborne allergens using the PolyCheck method. Results: IgE-mediated sensitization has been diagnosed in 20.3% of children with CD (CD/A). In the CD/A group, 58.3% of children were sensitized to food and 66.7% to airborne allergens. Further, 41.7% of patients with CD and allergy reported gastrointestinal tract symptoms associated with the ingestion of sensitizing foods. Analysis of the clinical status revealed that the incidence of other allergic disorders in the CD/A group was as follows: atopic dermatitis (33.3%), asthma (25.0%), and allergic rhinitis (16.7%). The percentage of eosinophils was significantly higher in the CD/A group than in the CD group (0.33±0.25 vs. 0.11±0.09; p=0.006). Conclusion: The diagnosis of CD does not exclude FA. The gastrointestinal symptoms in children with CD may be the result of both CD and FA; therefore, children with CD should be evaluated for the presence of FA regardless of age.

• Corrosion Science and Technology
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• v.21 no.3
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• pp.230-241
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• 2022

Austenitic stainless steel has been widely used because of its good corrosion resistance and mechanical properties. However, intergranular corrosion can occur if the alloy is welded or aged. The objective of this study was to determine intergranular corrosion behaviors of austenitic medium carbon (0.05 wt%) and low carbon (0.02 wt%) stainless steel aged at several conditions. Alloys were evaluated according to ASTM A262 Practice A, ISO 12732 DL-EPR (double loop-electrochemical potentiokinetic reactivation) test, and ASTM A262 Practice C. The degree of sensitization and intergranular corrosion rate were obtained. The relationship between the degree of sensitization and the intergranular corrosion rate showed a very large fluctuation. Such behavior might be related to whether two-dimension tests or three-dimension tests were performed. On the other hand, regardless of carbon content of alloys, when the intergranular corrosion rate increased, the degree of sensitization also increased. However, the DL-EPR test showed a higher sensitivity than the Huey test for differentiating the intergranular corrosion property at a low intergranular corrosion rate, while the Huey test had a higher sensitivity than the DL-EPR test for distinguishing the intergranular corrosion property at a high intergranular corrosion rate.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.22 no.3
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• pp.372-379
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• 2013

In this study, we investigated the traits of the clad metals used in hot-rolled clad steel plates. We examined the sensitization and mechanical properties of STS 316 steel plate and carbon steel (A516) under the specific circumstances of post heat treatment and whether a weld was multilayered and thick or repeated because of repairs. The test conditions were as follows. The clad steel plates were butt-welded using FCAW/SAW, and the heat treatment was conducted at $625^{\circ}C$, for 80, 160, 320, 640, or 1280 min. The change in the corrosion resistance was evaluated in these specimens. In the case of the carbon steel (A516), as the heat treatment time increased, the annealing effect caused the tensile strength to decrease. The micro- hardness gradually increased and then decreased after 640 min. The elongation and contraction of the area increased gradually. An oxalic acid etch test and EPR test on STS316, a clad metal, showed a STEP structure and no sensitization. From the test results for the multi-layered and repair welds, it could be concluded that there is no effect on the corrosion resistance of clad metals. In summary, the purpose of this study was to suggest some considerations when developing on-site techniques and evaluate the sensitization of stainless steels.

• Journal of radiological science and technology
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• v.6 no.1
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• pp.43-67
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• 1983

In order to increase the sensitivity of the photographic emulsion, one of the chemical sensitizers hydroxymethane sulfonic acid sodium salt(HMS) was applied through the chemical ripening process and following four types of emulsion were prepared. Their grain sizes were determined through the electron microscope. The ranges of average grain sizes were $0.05-0.40{\mu}m$ for silver chlorobromide emulsion of low sensitivity $0.10-0.45{\mu}m$ for the silver chlorobromide emulsion of medium sensitivity, $0.60-1.05{\mu}m$ for the silver iodobromide emulsion of high sensitivity, and $0.90-1.55{\mu}m$ for the ammonical silver iodobromide emulsion of high sensitivity. Through the reduction sensitization with HMS, they showed significant sensitivity enhancement by the increment of HMS concentration above pH 5.5 and pBr 3.0. Required sensitivity was obtained above pH 6.2 and pBr 3.3 when sulfur and gold sensitization were applied. Fog formed along the reduction sensitization was successfully prevented by the addition of 5.5-dimethylhydantion.