• Fisheries and Aquatic Sciences
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• v.27 no.5
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• pp.314-328
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• 2024

The mechanism for the elimination of xenobiotics undergoes three different phases of reactions in organisms. Among these, glutathione S-transferases (GSTs) are classified as phase II detoxification enzymes, catalyzing the conjugation of electrophilic substrates to glutathione or reduced hydroperoxides. This study aimed to investigate the molecular characteristics, detoxification functions, and immune responses of GST omega (LhGSTO1) and kappa (LhGSTK1) in redlip mullet. The open reading frames of LhGSTO1 (720 bp) and LhGSTK1 (687 bp) encoded proteins of 239 and 228 amino acids, respectively. Sequence analysis revealed that LhGSTO1 and LhGSTK1 possessed GSH-binding sites in their N-terminal domains. Substrate-binding sites in the C-terminal domain were exclusively identified in LhGSTO1. In the tissue-specific transcription profile analysis, both LhGSTO1 and LhGSTK1 were ubiquitously expressed in all tissues of healthy mullets. Temporal expression analysis of LhGSTO1 and LhGSTK1 in the blood showed that their expression was significantly modulated by polyinosinic:polycytidylic (poly I:C), lipopolysaccharide (LPS), and Lactococcus garvieae. Different chemical and cellular assays were performed to assess the detoxification and cellular protective abilities of the two proteins. A substrate specificity test using the recombinant proteins revealed that both proteins possessed specific activity toward 1-chloro-2,4-dinitrobenzene (CDNB). In the disk diffusion assay, the smallest clearance zones were observed for LhGSTO1 and LGSTK1 against CdCl₂. In the cell protection assay, both LhGSTO1 and LhGSTK1 showed significant Cd detoxification ability compared to the control. Collectively, these results demonstrate that GST omega and kappa are involved in host defense against immune stimulants and xenobiotics in redlip mullet.

• Journal of Microbiology
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• v.45 no.6
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• pp.572-577
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• 2007

To enhance therapeutic potential of murine monoclonal antibody, humanization by CDR grafting is usually used to reduce immunogenic mouse residues. Most humanized antibodies still have mouse residues critical for antigen binding, but the mouse residues may evoke immune responses in humans. Previously, we constructed a new humanized version (AKA) of mouse CC49 antibody specific for tumor-associated glycoprotein, TAG-72. In this study, to select a completely human antibody light chain against TAG-72, guided selection strategy using phage display was used. The heavy chain variable region (VH) of AKA was used to guide the selection of a human TAG-72-specific light chain variable region (VL) from a human VL repertoire constructed from human PBL. Most of the selected VLs were identified to be originated from the members of the human germline VK1 family, whereas the VL of AKA is more homologous to the VK4 family. Competition binding assay of the selected Fabs with mouse CC49 suggested that the epitopes of the Fabs overlap with that of CC49. In addition, they showed better antigen-binding affinity compared to parental AKA. The selected human VLs may be used to guide the selection of human VHs to get completely human anti-TAG72 antibody.

• Korean Journal of Poultry Science
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• v.9 no.1
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• pp.35-42
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• 1982

Data pertaining to the first generation of a Synthetic White Leghorn flock were used to estimate the heritabilities of and genetic correlation between partial egg production(egg number or percentage) or diversely segmented part records and other traits such as age at sexual maturity, residual and annual egg production, and to compare the expected genetic gain from selection on partial egg number or partial percent production with correlated response in other traits. The estimated heritabilites for six measures of egg Production were ranged from 29 to 35, while heritability for age at sexual maturity (SM) was intermediate (48). Genetic correlations between partial egg number (P) and annual egg number. (A), and partial percent production (P') and annual percent production (A') were 51 and 72, respectively. Genetic correlation between P and SM was estimated largely negative (-.64), while correlation bettween P' and SM was positively intermediate(34). In comparing direct response from selection on partial production (P or P') with another response in correlated trait, selection on P would be 25% more efficient than selection of P' for improving A, while selection of P' would be 94% more efficient than selection P for improving A' For shortening SM selection of P would be 98% more efficient than selection on P'.

• Journal of Korean Academy of Nursing
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• v.37 no.2
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• pp.213-220
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• 2007

Purpose. The purpose of this review is to explore cultural influences on the experiences of Korean, Korean American, and Caucasian American family caregivers caring for frail older adults in terms of the selection of a primary caregiver, caregiving motivation, support/help-seeking, and negative emotional responses (depression and burden). Methods. Seven electronic databases were searched to retrieve studies from 1966 to 2005. Thirty-two studies were identified. Results. This review supported cultural influences on the selection of primary caregiver, caregiving motivation, and support/help-seeking among the three caregiver groups. In Korean caregivers, the major primary caregivers were daughters-in-law while among Korean American and Caucasian American caregivers, the major primary caregivers were daughters or spouses. As a major caregiving motivation, Caucasian American care￢givers reported filial affection while Korean caregivers and Korean American caregivers reported filial obligation. Korean caregivers reported higher extended family support, while Caucasian American caregivers reported higher utilization of formal support. Korean caregivers showed the highest levels of depression followed by Korean American caregivers and Caucasian American caregivers. Conclusion. In order to develop culturally appropriate interventions and policies, more research is needed to further explain these differences among the three groups, especially regarding support/help-seeking and negative emotional responses.

• Smart Structures and Systems
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• v.26 no.4
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• pp.419-433
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• 2020

Investigation of structural integrity has been a critical issue in the field of civil engineering for years. Visual inspection is one of the most available methods to explore deteriorative components in structures. Still, this method is not applicable to invisible damage of structures. Alternatively, system identification methods are capable of tracking modal properties of structures over time. The deviation of these dynamic properties can serve as indicators to access structural integrity. In this study, a modal tracking technique using frequency-domain system identification from seismic responses of structures is proposed. The method first segments the measured signals into overlapped sequential portions and then establishes multiple Hankel matrices. Each Hankel matrix is then converted to the frequency domain, and a temporal-average frequency-domain Hankel matrix can be calculated. This study also proposes the frequency band selection that can divide the frequency-domain Hankel matrix into several portions in accordance with referenced natural frequencies. Once these referenced natural frequencies are unavailable, the first few right singular vectors by the singular value decomposition can offer these references. Finally, the frequency-domain stochastic subspace identification tracks the natural frequencies and mode shapes of structures through quick stabilization diagrams. To evaluate performance of the proposed method, a numerical study is carried out. Moreover, the long-term monitoring strong motion records at a specific site are exploited to assess the tracking performance. As seen in results, the proposed method is capable of tracking modal properties through seismic responses of structures.

• Smart Structures and Systems
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• v.21 no.5
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• pp.669-675
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• 2018

Under braking forces of a freight train, there are great longitudinal structural responses of a large freight railway cable-stayed bridge. To alleviate such adverse reactions, viscous dampers are required, whose parametric selection is one of important and arduous researches. Based on the longitudinal dynamics vehicle model, responses of a cable-stayed bridge are investigated under various cases. It shows that there is a notable effect of initial braking speeds and locations of a freight train on the structural responses. Under the most unfavorable braking condition, the parameter sensitivity analyses of viscous dampers are systematically performed. Meanwhile, a mixing method called BPNN-NSGA-II, combining the Back Propagation neural network (BPNN) and Non-Dominated Sorting Genetic Algorithm With Elitist Strategy (NSGA-II), is employed to optimize parameters of viscous dampers. The result shows that: 1. the relationships between the parameters of viscous dampers and the key longitudinal responses of the bridge are high nonlinear, which are completely different from each other; 2. the longitudinal displacement of the bridge main girder significantly decreases by the optimized viscous dampers.

• The Mathematical Education
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• v.46 no.2 s.117
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• pp.227-237
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• 2007

This research analyzes students' response types in the creativity assessment by using pattern block, geoboard, and pantomino. 74 students from third grade to sixth grade participated in this research. 15 minutes were given to pattern block and geoboard questions. 74 students showed 393 answers in pattern block question and 590 answers in geoboard question. In pantomino, 20 minutes were given and 54 students showed 443 types of answers. The results are as follows: First, in the students' responses, tendency of using particular piece or figure, which presents conjoining in a piece selection and positioning, showed strongly. For example, usage of hexagon and trapezoid pieces were higer in pattern block and usage of L, P, and I pieces were higer in pentomino. Second, it is confirmed that creativity's subordinate factors, fluency, flexibility, and originality, are separate from each other. To illustrate, in pattern block, three students', who showed 11 types of responses in fluency, flexibility responses were each 5, 6, and 8 types. Specially, among those studenys, only one could achieve a point in originality. Third, students' response types categorized in this research could be used for a bae-data to mark grades on originality.

• Journal of Korean Dental Hygiene Science
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• v.4 no.1
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• pp.29-37
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• 2021

Background: The selection of an occupation is typically based on individuals' personalities and the characteristics of occupations, which significantly affect occupational consciousness. The present study aimed to enhance the occupational achievement level of and provide fundamental data for student counseling in order to develop competitive professional workers by understanding the occupational consciousness of freshmen and motivating them as dental hygienists with career development plans, as freshmen majoring in dental hygiene eventually play a significant role in the field of dentistry as dental hygienists. Methods: The surveys were distributed to 160 freshmen in the dental hygiene department and were subsequently collected. The data from 142 surveys were used for analysis, as 18 surveys were excluded due to insincere responses. The survey contents included questions related to major selection and satisfaction, including motives for selecting a dental hygiene major, prior knowledge on a dental hygiene major and a career as a dental hygienist, satisfaction level of the major, and reasons for dissatisfaction in cases if applicable, as well as questions related to occupational consciousness, including career prospects for dental hygienists, opinions on the occupation, and conditions of job selection. Results: High employment rate with good salary level ranked highest (43.7%) among motives to apply the dental hygiene major, followed by the desire to be a professional worker (21.1%) and recommendation by acquaintances. Of those who responded, 50.7% indicated a normal level of satisfaction with the major, and 69.9% responded that they had prior knowledge regarding the dental hygiene major and/or field of dental hygiene. These results may be due to youth unemployment and the occurrence of job preparation immediately after students enter university, which is a result of the difficulty in job seeking. In terms of career prospects, 48.6% of students responded with "growing a little bit," followed by "growing a lot" (28.9%), "no difference from now" (21.1%), and "other" (1.4%). Regarding opinions on the occupation, 65.5% responded that occupation was an tool with which to make and income or a living, 23.2% responded that occupation was for dreams and self-realization, and 11.3% responded that occupation was for success in life and maintaining social status. Regarding the conditions of job selection, the responses included that the workplace had good working conditions (39.4%), good interpersonal relationships (21.8%), and a higher salary (18.3%). This may reflect the change in work ethics among university students, according to the trend of the times. Conclusion: Based on the results of the present study, we found that educational guidance to enhance the level of satisfaction with the major, and career guidance to understand and apply the clear vision and long-term job security are necessary.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.41 no.5
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• pp.251-258
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• 2004

This paper proposes a method for estimating the illuminant chromaticity using the distributions of the camera responses obtained by a CCD camera in a real-world scene. Illuminant estimation using a highlight method is based on the geometric relation between a body and its surface reflection. In general, the pixels in a highlight region are affected by an illuminant geometric difference, camera quantization errors, and the non-uniformity of the CCD sensor. As such, this leads to inaccurate results if an illuminant is estimated using the pixels of a CCD camera without any preprocessing. Accordingly, to solve this problem the proposed method analyzes the distribution of the CCD camera responses and selects pixels using the Mahalanobis distance in highlight regions. The use of the Mahalanobis distance based on the camera responses enables the adaptive selection of valid pixels among the pixels distributed in the highlight regions. Lines are then determined based on the selected pixels with r-g chromaticity coordinates using a principal component analysis(PCA). Thereafter, the illuminant chromaticity is estimated based on the intersection points of the lines. Experimental results using the proposed method demonstrated a reduced estimation error compared with the conventional method.

• Journal of Korea Society of Industrial Information Systems
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• v.6 no.1
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• pp.48-55
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• 2001

The survey was carried out for the student sin colleges of SangJi University for analyzing the parameters affecting the selection of ones cultural studies and major subjects. The number of respondents was 204, and the responses were computer-processed utilizing Excel and statistical software SPSS. The attributes of main factors were composed of House of Quality(HOQ) that is basic matrix of Quality Function Development. From the survey analysis, selection criteria for ones cultural studies and major subjects are as follows. In selecting ones cultural studies, teaching methodology is the most important factor than assignment, the percentage of attendance and academic credit. And in case of major subjects, no other factors than the recommendation by seniors were significantly affected ones subject selection.