• 한국대기환경학회지
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• 제22권5호
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• pp.642-652
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• 2006

In order to understand the characteristics of surface ozone concentration and high $O_3$ concentration days, regional data from seven air quality monitoring stations which were operated by local governments were analyzed Regional characteristics of $O_3$ concentration were analyzed with the data of $O_3$ concentration and the characteristics of $O_3$ generation and weather conditions by the selection of the days in which the concentration was higher than 80 ppb. In the case of daily variation, the lowest $O_3$ concentration was shown in all regions from 7am to 8am and the highest around at 4 pm. The monthly variation of mean $O_3$ concentration and ${\Delta}O_3$ values revealed a reducing pattern in July and August following the peak in June, and again a gradual increasing trend in September and October. The result shows that the amount of ozone is dependent on photochemical reaction. The days of $O_3$ generated more than 80 ppb in the region of Gwangyang-bay were 544 days(1,760 hrs). The frequency of occurrence in the region revealed a strong pattern with the order of Samil-dong, Jinsang, and Gwangmu-dong stations in the Gwangyang region. However, Tein-dong, which is the nearest station to air pollution material generation source, showed the lowest frequency in the study area. Consequently, the meteorological parameters which can easily generate the high concentration of $O_3$ in the region of Gwangyang-bay are characterized as follows; atmospheric temperature which is higher than $19^{\circ}C$, relative humidity with the range of $60{\sim}85%$, the less average wind velocity than 5 m/s, cloud cover which is less than 5/10, and the more duration of sunshine than 8 hours.

• Ocean and Polar Research
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• 제29권4호
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• pp.379-389
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• 2007

This paper presents an attempt to determine natural background levels of heavy metals which could be used for assessing heavy metal contamination. For this study, a large archive dataset of heavy metal concentration (Cu, Cr, Ni, Pb, Zn) for more than 900 surface sediment samples from various Korean coastal environments was newly compiled. These data were normalized for aluminum (grain-size normalizer) concentration to isolate natural factors from anthropogenic ones. The normalization was based on the hypothesis that heavy metal concentrations vary consistently with the concentration of aluminum, unless these metals are of anthropogenic origin. So, the samples (outliers) suspected of receivingany anthropogenic input were removed from regression to ascertain the "background" relationship between the metals and aluminum. Identification of these outliers was tested using a model of predicted limits at 95%. The process of testing for normality (Kolmogorov-Smirnov Test) and selection of outliers was iterated until a normal distribution was achieved. On the basis of the linear regression analysis of the large archive (please check) dataset, background levels, which are applicable to heavy metal assessment of Korean coastal sediments, were successfully developed for Cu, Cr, Ni, Zn. As an example, we tested the applicability of this baseline level for metal pollution assessment of Masan Bay sediments.

• 농촌지도와개발
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• 제19권3호
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• pp.649-672
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• 2012

The government established Special Accounts for Balanced National Development in 2005, and the Metropolitan Council has enacted and executed ordinance relevant to special accounts to relieve regional unbalanced growth and to promote regional balanced growth. This study derived a political implication through the comparison analysis of the background of establishment and its purpose, the selection criteria of support target, finance scale and tax revenue finance, appropriation target and finance distribution, etc. on the basis of Chungcheongnam-do Special Accounts for Balanced Development and Jeollabuk-do Eastern part Special Accounts among the special accounts implemented in metropolitan council. The major conclusions of this study are as follows. First, the transition from the concept of balanced development to specialized development is necessary for the development of distressed region. Second, the pure wastefulness ratio of the local government's tax revenue finance of special accounts designed concentrating upon special accounts for Wide-area Autonomous Communities development should be gradually expanded. Third, Special Accounting budget should be supported to promote specialized development through the selection and concentration centering around comparatively advantageous resource within the region. Fourth, the strategic special accounts budget application system of cities and counties to utilize the projects of the government and the province to achieve the goal of cities and counties should be prepared.

• Plant Biotechnology Reports
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• 제2권1호
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• pp.47-58
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• 2008

To select agronomically useful transgenic plants, a large number of transgenic events are initially produced, gene transfer confirmed, and advanced to obtain homozygous lines for testing in field trials. Direct in planta assays for identifying the transgene carriers in the segregating progeny are based on the activity of selectable marker gene and are easy, simple and inexpensive. For this purpose, expression of bar gene as measured by tolerance to damage by glufosinate ammonium, the active ingredient in the herbicide BASTA, was investigated. Dose damage curves were generated by leaf paint tests with BASTA on four genotypes of sorghum. Transgenic plants were characterized in terms of sensitivity to the concentration of glufosinate ammonium. In transgenics, symptoms of BASTA swab tests at different growth stages and PCR analysis for cry1B were carried out and correlated. Germination tests could not be employed for large scale evaluation of transgenic progeny because of mortality of tolerant seedlings after transplantation to soil. Based on the above findings, a simple, inexpensive, time-saving, two-step scheme for effective evaluation of transgenics and their progeny containing bar gene as selection marker using BASTA swab tests is described.

• 한국지하수토양환경학회지:지하수토양환경
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• 제20권7호
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• pp.13-22
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• 2015

In this study, we have developed the performance evaluation model for the optimal soil remediation technology selection. Performance evaluation model is composed in the evaluation of two steps. In the first stage, the candidate technologies are derived according to the conditions of drilling, type and concentration of pollutants, and the saturated/unsaturated of target site. In the second stage, each individual candidate technology is evaluated by performance evaluation model. The performance evaluation model has 5 groups of evaluation items and 12 evaluation items which have their own evaluation index and their own weights through the AHP approach surveying 40 experts. From the case study of actual design cases, the applicability of the performance evaluation model was confirmed.

• Bulletin of the Korean Chemical Society
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• 제26권12호
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• pp.2033-2037
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• 2005

In vitro selection was used to isolate $Mg^{2+}$-dependent self-cleaving ribozymes with cis-cleavage activity from a pre-tRNA library having 40-mer random sequences attached to 5'-end of E. coli $tRNA^{Phe}$. After 8 rounds of SELEX (Systematic Evolution of Ligands by Exponential Enrichment), RNA molecules which can self-cleave at the high concentration of $Mg^{2+}$ were isolated. The selected ribozymes can carry out the self-cleavage reaction in the presence of 100 mM $Mg^{2+}$ but not in 10 mM $Mg^{2+}$. The cleavage sites of the ribozymes are located at +3 and +4 of $tRNA^{Phe}$, compared with +1 position of 5'-end cleavage site of pre-tRNA by RNase P. New RNA constructs deprived of its D stem-loop, anticodon stem-loop, variable loop and T stem-loop, respectively showed the cleavage specificity identical to a ribozyme having the intact tRNA structure. Also, the new ribozyme fused with both a ribozyme and $tRNA^{Leu}$ showed the cleavage activities at the various sites within its sequences, different from two sites of position +3 and +4 observed in the ribozyme with $tRNA^{Phe}$. Our results suggest that the selected ribozyme is not structural-specific for tRNA.

• 한국연초학회지
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• 제21권1호
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• pp.82-88
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• 1999

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single FI hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC95, was used to develop the 30 anther derived dihaploid (ADH) lines and maternally-derived dihaploid (MDH) lines utilizing Nicotiana africana, respectively. As compared with mid-parent, mean of ADH lines showed increase in number of leaves, delay in days to flower and narrow in width of leaf. However, no significant differences in the other investigated characters were recognized. MDH lines also showed narrow width of leaf, while no significant differences in the other characters were observed. The variations of the investigated characters were generally greater in ADH than MDH lines. MDH lines had higher height of plant and shorter days to flower than ADH lines, while the other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were height of plant, number of leaves, days to flower, and yield, while those showing relatively low value were length of leaf, width of leaf, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(ADH, MDH) and correlated responses associated with selection for yield were estimated. Height of plant, width of leaf, days to flower, and concentration of reduceing sugar would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• 한국약용작물학회지
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• 제11권4호
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• pp.289-297
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• 2003

Using Agrobacterium-me야ated transformation method the auxin-regulated cotton GST (Gh-5) constructs were used to transform Rehmannia glutinosa L. The PCR analysis was conducted to verify transgenicity. Based on the PCR analysis, there was verified that the 988 bp DNA band had showed in transgenic plant genomes in PCR anaJysis using Gh5-1 and Gh5-2 primers. The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of Chinese foxglove (Rehmannia glutinosa L.) were investigated. Factors such as cocultivation period, use of acetosyringone, postcultivation in darkness, and different kanamycin concentrations for selection were assessed. In vitro regeneration, the number of leaves, shoot lengths and numbers on MS medium were superior to on B5 and WPM medium, and the shoot formation rate was highest level of 95% in cultured base part containing leaf stalk. Addition of acetosyringone at concentration of $200{\mu}M$ to cocultivation medium and 3-day of cocultivation improved transformation frequencies. Exposure of explants to darkness for 4 weeks on selection medium resulted in further increased the regeneration frequency of transgenic shoots. In PCR analysis, the amplified fragments of Gh5 gene were detected (988 bp), and GST-expressing transgenic R. glutinosa L. plants had approximately three-fold higher activity in leaf extracts compared with control plant.

• 조명전기설비학회논문지
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• 제19권7호
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• pp.67-72
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• 2005

WPAN(Wireless Personal Area Networks)에서는 현재 동작중인 PNC(Piconet Coordinator)가 피코넷을 떠나거나 종료하고자 할 때, 피코넷 내의 PNC 능력을 가진 디바이스에게 PNC 역할을 양도할 수 있다. 그러나 WPAN의 권고안에서는 PNC 핸드오버를 위한 PNC 후보 선택에 대해서는 디바이스 성능 정보를 비교하여 선택하는 방안만을 기술되어 있으며, 이에 대한 구체적인 선택 방법은 포함하고 있지 않다. 본 논문에서는 피코넷 NC가 피코넷 내의 다른 디바이스에게 PNC 기능을 양도하게 될 때에 피코넷의 데이터 처리성능을 최적화 될 수 있게 PNC 수용 디바이스 개수와 밀집도를 고려하여 PNC를 선택하는 방안에 대해 제안한다. 수치 해석의 결과에서 보면 디바이스들이 집중되어 있을수록 평균 데이터 처리량이 증가되는 것을 나타내었고, 최고 두 배까지 향상되었다. 따라서 PNC 후보 선발시 서비스 디바이스 개수와 밀집도를 같이 고려해야만 피코넷 성능을 높일 수 있다.

• Journal of the Korean Data and Information Science Society
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• 제26권4호
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• pp.915-921
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• 2015

본 연구는 젖소 35,645두의 검정기록, 총 294,834를 이용하여 1회 착유량과 아세톤 및 ${\beta}$-히드록시부틸산 (BHBA) 함량의 (공)유전력을 추정하기 위하여 수행되었다. 아세톤과 BHBA의 평균 함량은 원유 1리터당 각각 $135.54{\pm}96.29{\mu}mol$과 $61.08{\pm}66.76{\mu}mol$였으며, 상위군과 하위군간 아세톤과 BHBA 함량의 차이는 각각 $6.12{\mu}mol/L$과 $4.35{\mu}mol/L$로서 모두 유의적인 차이(p<0.01)를 나타내었다. 1회 착유량의 유전력은 0.18~0.21, 아세톤 함량은 0.11~0.13과 BHBA 함량은 0.01~0.02의 범위에서 추정되었다. 아세톤과 BHBA의 표현형상관과 유전상관은 각각 0.44와 0.48로 추정되었으며, BHBA 함량이 $500{\mu}mol/L$ 이상부터는 아세톤과의 상관관계가 현저히 낮아지는 현상을 나타내었다. 하위군에서 1회 착유량에 대한 아세톤과 $Log_e$아세톤의 공유전력은 각각 0.02와 0.16으로 추정된 반면, BHBA와 $Log_eBHBA$의 공유전력은 각각 0.26과 0.32로 아세톤의 추정치보다 높게 나타났으며, 이러한 결과는 전체자료로 추정된 결과와도 일치하였다. 따라서 아세톤 함량보다는 1회 착유량과 공유전력이 높은 BHBA 함량이 1회 착유량에 대한 선발을 통하여 간접선발 반응을 유도하는데 더 효과적이며, 지시형질로서 바람직하였다.