Twenty-five species of fungi were deleted and identified from 27 kinds of the intercepted seeds from 5 countries. Eight species of fungi including Fusariun moniliforme were detected from the imported calabash seed (Lagenaria siceraria). These seeds were the most contaminated among the intercepted reeds tested. Six kinds of seeds including larch(Larix kaemferi) were disinfected with Vitathiram, Benlate-T and Captan. Rhynchosporium was detected from seeds treated with Vitathiram whereas Alternaria spp. were detected from seeds treated with Benlate T or Captan. Six genera of fungi were detected from the untreated seeds. In the case of germination, it seem that seed disinfectants do not have any damage on germination of seed.
Ginseng seeds were treated with different autoclaving temperatures and autoclaving times, and extracted with 80% methanol to measure changes in antioxidant activity. The antioxidant activity of ginseng seeds treated by autoclaving was measured by 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, 2,2'-aziono-bis(3-ethylbenzthiazoline)-6-sulfonic acid radical scavenging activity, superoxide dismutase SOD-like activity, ferric reducing antioxidant power (FRAP), and total phenolic compound content. As autoclaving temperature and time were increased, the L lightness value decreased and the redness value tended to increase. Total phenolic compound content was about three times higher in ginseng seeds treated with autoclaving at 130°C than in ginseng seeds that were not treated. DPPH radical scavenging activity and ABTS radical scavenging activity increased as autoclaving temperature and time were increased. In particular, when the concentration was 100 ppm, the ABTS radical scavenging activity was 91.80% in ginseng seeds treated by autoclaving at $130^{\circ}C$, which was the highest antioxidant activity. FRAP and SOD-like antioxidant activity tended to increase significantly as autoclaving temperature and time were increased.
Low field NMR was employed to discriminate the geographical origin of sesame seeds from Sudan, China, and Korea. Sudan sesame seeds had the lowest contents of moisture and crude fat. Chemical components of Korean and Chinese sesame seeds were similar, whereas relaxation times $(T_1-IR,\;T_1-SR)$ measeured through spin-lattice relaxation pluse techniques using 20 MHz NMR showed significant difference (p0.05). Canonical discriminant analysis could be used to identify the habitat of sesame seeds with over 90% accuracy of NMR results. Non-destructive and fast NMR techniques can be applied to classify Korean sesame seeds from those of other origins.
Proceedings of the Plant Resources Society of Korea Conference
/
2019.10a
/
pp.76-76
/
2019
The purpose of this study was to evaluate the antioxidant and whitening action of Fagopyrum esculentum and F. tataricum buckwheat seeds depending on their germination time. In a previous study, we reported significant changes in sprout yield and phytochemical content in ethanol extracts from F. esculentum and F. tataricum seeds with increase in germination times. DPPH radical scavenging activities of F. tataricum increased with increasing germination time, whereas that of F. esculentum decreased. Next, we investigated anti-melanogenic activities of these species by estimation of melanin content and tyrosinase activity. Inhibition of melanin production in ${\alpha}-MSH$ (${\alpha}$-melanocyte stimulating hormone)-induced B16F10 cells by extracts from these seeds were analyzed. Among the two, F. tataricum extracts were characterized by higher inhibitory activity against melanin production. In addition, when B16F10 cells were incubated with L-DOPA for detection of in situ tyrosinase activity, F. tataricum and F. esculentum extracts were observed to reduce melanin production in cells. Taken together, these results indicate that extracts from buckwheat seeds could influence cellular processes via modulation of tyrosinase activity. Hence, buckwheat seeds could be utilized as whitening agents in the cosmetic industries and as therapeutics for hyperpigmentation disorders in a clinical setting.
An evaluation was carried out on variability in morphology of fruits and seeds (number and weight) of Tetrapleura tetraptera (Schumach. and Thonn.) Taub. from different populations across its distribution range in Nigeria. Bulk fruit samples were collected and examined for variations in morphological characters. Differences in morphological character of fruits and seeds among the populations were determined using analysis of variance at 5% level of probability. The relationships among morphological characters were determined using Pearson correlation coefficient (r). Significant variations (p<0.05) existed among T. tetraptera populations for all the evaluated characters: fruit length, fruit width, number of seeds per fruit and seed weight. A positive significant strong correlation (r=0.96) was found between seed weight and number of seeds per fruit, while no correlation existed between fruit length, width and number of seeds. Seed weight was positively correlated with minimum altitude (r=0.97) and maximum altitude (r=0.99) of seed populations. Number of seeds was also significantly correlated with maximum altitude (r=0.965). There was no significant correlation between geo-climatic variables and fruit dimensions (length and width). Observed variations in morphological traits within and across populations of T. tetraptera may be used as proxy to estimate genetic diversity and selection of superior trees for improved productivity.
Kim, Sun-Lim;Lee, Yeong-Ho;Chi, Hee-Youn;Lee, Sun-Joo;Kim, Si-Ju
KOREAN JOURNAL OF CROP SCIENCE
/
v.52
no.3
/
pp.348-357
/
2007
The 117 soybeans seeds were collected from the nine provinces of Korea, and protein and lipid contents, and fatty acid composition levels were evaluated to investigate their relationship. The 100-seed weights of the black soybeans were varied $27.7{\sim}33.1g$, while the 100-seed weight of yellow soybeans were varied $24.6{\sim}36.6g$. Protein and lipid content of the 117 soybean seeds was 38.3% and 17.8%, respectively Protein contents of the 59 black soybean seeds (38.6%) were significantly higher than those of the 58 yellow soybean seeds (37.9%). However, lipid contents of the black soybean seeds (17.6%) were lower than those of the yellow soybean seeds (18.1%). Linoleic and oleic acid composition levels of the 117 soybean seeds were 53.75% and 22.08%. Unsaturated fatty acid levels of soybean seeds showed a statistically significant variability among the nine provinces of Korea, however, the differences were not found in the linoleic (18:2) and oleic acid (18:1) levels. Therefore, it was considered that the significant variability of unsaturated fatty acid were mainly due to the variations of linolenic acid (18:3) level. The composition levels of linoleic, oleic, palmitic (16:0), and linolenic acid in the yellow soybean seeds were 53.43%, 22.73%, 12.23%, and 8.24%, while those of the black soybean seeds were 54.13%, 21.48%, 12.47%, and 8.31%. Obtained results suggested that fatty acid composition levels were varied and possibly influence by the phenotype of seed coat colors. Oleic acid, mono-saturated fatty acid, showed the most remarkable variability between yellow and black soybean seeds, and the composition levels were higher in the yellow soybean seeds. Relationship between unsaturated fatty acid levels and 100-seed weights in the yellow soybean seeds showed a negative correlation (r=-0.513, P<0.01), but no relationship $(r=0.154^{ns})$ was observed in the black soybean seeds.
1) The purpcse of the present study is to investigate the effects of culture filtrates of Fuarsium oxysporum f. vasinfectum which is known to produce wilt toxin (fusaric acid) on the germination of host plants (sesame, cotton) and non-host plants (wheat, rice). 2) The experiment on the germination of sesame, cotton, wheat and rice seeds in the seed beds separately added with culture filtr ates of 10 differential strains of Fusarium oxysporom f. vasinfectum demonstrated that culture filtrates of most strains of the fungus inhibit or retard the germination of seeds of 4 plants used in this study while those of a few strains do not give notable influence on the germination of seeds of those plants. a) Culture filtrates of strain 201 of the fungus strongly inhibited the germination of seeds of those plants in nearly same degree, but culture filtrates of the other strains, 281, 321, etc., showed remarkable differences in the toxicity inhibiting or retarding the germination of the seeds of those plants. b) In general, sesame seeds are greatly susceptible, wheat and cotton seeds are moderately susceptible and rice seeds are resistant to the toxicity of culture filtrates of the fungus. 3) In the soil containing a number of differential strains of Fusarium oxysporum f. vasinfectum, the germination of seeds and also the growth of seedlings of non-host plants are possibly checked by the toxic substance, fusaric acid produced by the fungus.
This research was performed to develop mass propagation method of round-leaved sundew (Drosera rotundifolia L.) for improving horticultural usefulness. Seeds were collected around Hwaseong-si, Gyeonggi-do in October of 2011 and experimented upon while being dry-stored at 4 ± 1.0℃ (darkness). Seed length of a major and minor axis were 1.58 ± 0.060 and 0.21 ± 0.016 ㎜, respecrively, and weight of 1,000 seeds was 6.24 ± 0.172 ㎎. Seeds were thus classified as ‘dwarf seeds’. Regarding the dormancy type, since round-leaved sundew seeds were dormancy broken and germinated at 20~30℃ under the light condition after wet-chilling treatment for 12 weeks, seeds were estimated to have physiological dormancy. Germination conditions of dormancy broken seeds were found to be 20℃ and light condition (54.7%), but germination decreased at higher temperature. Percent germination (PG), germination energy (GE), mean germination time (MGT) and T50 were effectively improved by chemical treatment such as GA3 200 ㎎/L + kinetin 20 ㎎/L and wet-chilling treatment for 14 weeks. In conclusion these optimal conditions were thought to be a useful method for raising seedling uniformly.
Proceedings of the Korean Society of Crop Science Conference
/
2017.06a
/
pp.197-197
/
2017
In order to reduce the loss of perilla seeds at harvesting stage, different seeding methods and harvesting time had been applied to upland soil in NICS (National Institute of Crop Science), RDA, Milyang, Korea. The seeding methods were divided into hill and drill seeding, and harvesting time were 40%, 60%, 80%, and 100% color-changing of top cluster perilla (cv Deulsaem) seeds. Higher plant height and lowest flower cluster, thinner culm thickness was observed in drill seeding treated plot than hill seeding treated plot. However, field lodging occurred lower at drill seeding treatment (lodging degree 3) than hill seeding plot (lodging degree 5). Harvesting time affected the loss of perilla seeds and the loss rate of perilla seeds were investigated at different color-changing of top flower cluster. When seeds were harvested at 40%, 60%, 80%, and 100% color-changing of top cluster perilla seeds, the loss rate of hill and drill seeding showed no significant differences however the loss rated of 40, 60, 80, and 100 % color-changing of top flower cluster were 1.9% to 3.0%, 3.8% to 3.9%, 8.0% to 10.2%, and 16.1% to 22.7%, respectively. The harvesting time had no significant effects on the yield of perilla seeds. These results suggest that optimum harvesting time of perilla could be recommend at the 60% color-changing of top cluster to reduce the loss of perilla seeds.
Proceedings of the Korean Society of Crop Science Conference
/
2017.06a
/
pp.186-186
/
2017
Relay intercropping system of direct seeded rice and winter cereal is labor saving cultivation and has high land use efficiency in Japan. In this system, rice seeds are direct seeded into inter-row space of winter cereals (wheat or barley) in March or April. If the direct seeding of rice is conducted before stem elongation phase or using suitable seeder, these are little effect to yield of winter cereals. Though the seeds are generally thiuram treated, it's a matter that seedling establishment rate (SER) of direct seeded rice is low and unstable. The cause of low SER has not been revealed. In present study, with the aim to reveal causes of low SER, we conducted experiments and investigated the SER, and analyzed some factors that might affect SER. Experiment1: In 2015, 2016, we buried rice seeds underground, and investigated the transition of the seed survival rates (SSR). Seeds were thiuram-treated or non-treated. In 2 periods, SSR of thiuram-treated seeds were significantly higher than non-treated seeds. In 2016, thiuram-treated seeds were high in SSR (almost 75%) at April 30th, but low SER (10~27%) after harvest of winter cereals. Therefore, almost all of seed death might have been happen after germination. Analysis 1: We investigated the SER and cultivation conditions in Ibaraki pref. for several years. Meteorological factors were referred from the nearest point of AMeDAS. From mean temperature (MT) among 5days after and before the day, we divided the period of seeding ~June 20 to phase1~4. We defined each phase as below; Phase1: $MT{\leq}10^{\circ}C$, Phase2: $10^{\circ}C<MT{\leq}15^{\circ}C$, Phase3:$15^{\circ}C<MT{\leq}20^{\circ}C$, Phase4: $MT>20^{\circ}C$. We analyzed the correlation of SER and meteorological factors by each phase. Total number of days in phase 1~4 was significantly negative correlated with SER. In phase1, total rain fall and number of soil wetting days were significantly negative correlated with SER. In phase2~4, only MT was significantly positive correlated with SER. This result suggested that rainfalls in phase1 declined seed vigor to emergence from soil surface, by repeated water absorption and re-dry. From these present studies, it was suggested that one of factors of low SER of direct seeded rice in relay intercropping system is changing of water condition by rainfalls in phase1 ($MT{\leq}10^{\circ}C$). To improve SER, it's necessary to consider something seed treatments such as prevent water absorption during phase1. However, 58~60% of seeds seemed to die during May. It's suggested that, if seeds are thiuram treated, almost all of seeds can germinate underground, but the seed vigor to emergence from soil surface are insufficient. Further studies are needed to reveal the rest causes that is happening during emergence from soil surface.
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