The objectives of this research were to investigate the drought resistance as well as the growth of Wisteria floribunda seedlings with the soil water conditions. The seedlings for the research were grown in pots with strict water content control on a frame located outdoors. During the experiments, the soil water contents were adjusted to 5, 10, 20, 30, 40, 50, 60, and 70%. In addition, the effects of Rhizobium inoculation on the growth of seedlings were investigated. The experimental results are summarized as follows: 1. The seedlings in the pots with 5% soil water content withered to death due to the water stress. Withering or any distinct growth was not observed from the seedlings in the 10% soil water content. It can be inferred from these results that about 5% of soil water content is the wilting point of W. floribunda seedlings and about 10% is the critical soil water content of its growth in this experiment soils. Therefore, it seems that W. floribunda possesses a good drought resistance. 2. From the analyses of the main growth parameters such as stem elongation, diameter growth, leaf area growth and total dry weight, it was found that the seedling growth can be improved with increasing soil water contents. The relation between each growth parameter(Y) and the soil water contents(W) was well described by a quadratic equation, $Y=a+bW+cW^2$. 3. In soil water contents higher than 20%, the seedling growth(Y) was remarkable along with-its extended growing period, and related to the growing period(D) by a quadratic equation, $Y=a+bD+cD^2$. 4. The artificial inoculation of Rhizobiun promoted the growth of Wisteria floribunda seedlings. 5. Rhizobium was found to be more readily inoculated and to form more root nodules compared to seedlings grown in higher soil water contents.
This experiment was conducted to know the effects of foliar applicated bio-enzyme on the early growth of cucumber and red pepper seedlings. Bio-enzyme was manufactured by the culture and proliferation of Bacillus genus and foliar applicated by the concentration of 0.075, 0.15, 0.3 g.$\ell$-1. Foliar application of bio-enzyme had great influenced to the early growth both cucumber and red pepper seedlings. Optimum concentrations of bio-enzyme applicated for the growth of plant height were determined as of 0.075 g.$\ell$-1 in cucumber but in red pepper seedlings 0.15g.$\ell$-1 was more favored. However, foliar application of 0.15g.$\ell$-1 of bio-enzyme was recommended for both cucumber and red pepper seedlings. Especially, leaf area and total dry weight which are main indices of good seedling were highest in the plot of standard concentrations(0.15g.$\ell$-1)of bio-enzyme.
A series of experiments were carried out to know the effect of growth retardants on the growth of rice seedlings and ethylene evolution from the rice seedlings. The results suggested that the reduction rate in order of plant height was S-327 > BAS-106 > NTN-821 > PP-333 > CGR-811. The heigher concentration levels of growth retardants applied. the more effective reductions of plant height were found. Ethylene evolution from the rice seedlings was significantly negative correlated with plant hight of of seedlings in all treatments.
The inhibitory effects of mercury ions on the growth of barley seedlings were studied and the distribution of metal elements in the organs of treated plants was investigated by using synchrotron radiation induced X-ray emission (SRIXE). Although the treatment of mercury ions caused growth inhibition, the mercury-specific increase in variable fluorescence and the abolishment of energy-dependent quenching in broken barley chloroplasts as shown by Moon et at. (1992) were not observed in the leaves of growth-inhibited seedlings. Instead the treatment of mercury decreased Fmax and Fo values. However, Fmax/Fo ratio and photochemical and nonphotochemical quenching coefficients were not affected significantly. By SRIXE analysis of $10\mu\textrm{m}$ mercury chloride treated seedlings, accumulation of mercury in roots was observed after 1 hour of treatment and similar concentration was sustained for 48 hours. Relative contents of mercury was high in roots and underground nodes where seeds were attachedl but was very low in leaves. Iron and zinc were also distributed mainly in the lower parts of the seedlings. However after 72 hours of treatment the contents of these metals in roots decreased and their distribution became more uniform, which may lead to death of the plants. These results suggest that the observed inhibitory effects on barley seedlings upto 48 hours after the treatment is not due to direct damages in the photosynthetic apparatus, but due to its accumulation in roots and the consequent retardation of the growth of barley seedlings. The decrease in Fmax and Fo is probably due to the decrease in chlorophyll and protein contents caused by the retardation of growth. The observed slow expansion of primary leaves could be also explained by the retardation of growth, but the fluorescence induction pattern from the leaves did not show characteristic symptoms of leaves under water stress.
Proceedings of the Korean Society for Bio-Environment Control Conference
/
2004.11a
/
pp.139-143
/
2004
To evaluate the biological and physiological response of Picea koraiensis Nakai to elevated $CO_2$ and nitrogen.3-year old seedlings were planted in an ambient and 700 ppm $CO_2$ at low (2mM $NH_4NO_3$) or high nitrogen (16mM $NH_4NO_3$) supplying treatments for 3 months. Photosynthetic parameters were measured monthly. Seedlings were harvested at monthly intervals and growth parameters of root system, stem and needle fractions were evaluated. The result showed that height of the seedlings grown at both of elevated $CO_2Xhigh$ nitrogen and elevated CO2×low nitrogen supplying treatments increased significantly more than that of at ambient CO2 treatments. Seedlings grown at elevated $CO_2Xhigh$ nitrogen produced more root biomass than at elevated $CO_2Xlow$ nitrogen and ambient $CO_2Xhigh$ nitrogen treatments. This result suggested that the root growth response of Picea koraiensis seedlings was greater in elevated $CO_2{\times}high$ nitrogen regime, which is very important for carbon sequestration in soil. $A_{max}$ of the seedlings grown at elevated $CO_2Xhigh$ nitrogen increased during the three months significantly, and $A_{max}$ of the seedlings grown at the other three treatments decreased significantly, suggesting that the interaction between elevated $CO_2$ and high nitrogen supplying stimulates the $A_{max}$ of Picea koraiensis. $A_{max}$ of the seedlings grown at elevated $CO_2Xlow$ nitrogen showed higher than other three treatments in the first month of the experiment, but decreased in succedent two months, suggesting that elevated $CO_2$ promotes the photosynthesis of the seedlings. However long term growth in elevated $CO_2Xlow$ nitrogen supplying condition resulted in an acclimatory decreased in leaf photosynthesis.
Cinnamomum migao plants often face different degrees of drought in karst habitats, which can lead to plants' death, especially in the seedling stage. Widespread of arbuscular mycorrhizal (AM) fungi in karst soils have the potential to address this drought, which is a threat to C. migao seedlings. We inoculated C. migao seedlings with spores from Glomus lamellosum and Glomus etunicatum, two AM fungi widely distributed in karst soils, to observe seedling growth response after simulated drought. Our results showed that 40 g of G. lamellosum and G. etunicatum significantly promoted the growth of C. migao seedlings, 120 days after inoculation. Following a 15-day drought treatment, root colonization of the seedlings with G. lamellosum or G. etunicatum had lower the accumulation of malondialdehyde (MDA) and increased the accumulation of enzymes and osmotic substances in the seedlings. The relative water content in different organs (roots, stems, and leaves) of the drought-stressed seedlings was higher in plants with G. lamellosum or G. etunicatum than in plants without AM fungi colonization. Our results showed that inoculation with AM fungi was an effective means to improve the drought resistance of C. migao seedlings.
Proceedings of the National Institute of Ecology of the Republic of Korea
/
v.2
no.2
/
pp.76-81
/
2021
With continuous decline of Philippine forest cover, sustainable forest management and restoration are essential to restore destroyed forest ecosystems. Unfortunately, of ten most planted trees in reforestation projects in the Philippines, eight are exotic species, with large leaf mahogany (Swietenia macrophylla) being the most dominant. In this study, effect of Swietenia macrophylla in reforestation projects on native tree species was evaluated. Effects of S. macrophylla leaf litter, frequency, and canopy closure on the growth of the Philippine native species Pterocarpus indicus were investigated. Results showed that S. macrophylla leaf litter significantly inhibited the growth of P. indicus seedlings based on root collar-to-shoot height. The standardized growth rate of seedlings in plots without S. macrophylla leaf litter was significantly higher than the growth rate of seedlings in plots with leaf litter. Furthermore, there was no significant difference in the standardized growth rate of seedlings between plots without leaf litter and a control plot. On the contrary, S. macrophylla tree frequency and canopy closure showed no significant effect. These results attest to the negative effect of widely planted S. macrophylla to a valuable Philippine native tree P. indicus. With accumulating scientific evidence about negative effects of S. macrophylla on native trees, discontinued use in tree planting and reforestation efforts with active management of restoration sites previously planted with large leaf mahogany are needed.
Park, Hong Woo;Song, Jeong Ho;Kwon, Ki Bum;Lee, Ueong Ho;Son, Ho Jun
Korean Journal of Medicinal Crop Science
/
v.25
no.4
/
pp.238-243
/
2017
Background: The production method of ginseng seedlings for ginseng cultivation is very important to ensure healthy rooting system as well as high quality, and yield of the resultant plants. This study was carried out to compare the growth characteristics of 2-year-old ginseng plants that were produced from seedlings grown in self soil nursery (SSN), nursery soil (NS) or hydroponic culture (HC). Methods and Results: The shading prop used was composed of four-layered 4 polyethylene (blue 3 + black 1) shade screen. The management of main field was done by inserting oil cake (1,200 kg/10 a) and then allowing Sudan grass to grow for a year. Seedling transplantation was carried out on April 6. Root growth was measured on October 25. Root weight was observed to be excellent at 6.0 g, following SSN transplantation. Root length was 21.2 cm for HC seedlings, but these plants had a physiological disorder (i.e., rusty root), in 83.5% plants of this treatment. The ratio of PD/PT (protopanaxadiol saponins / protopanaxatriol saponins) was higher in NS seedlings. Plant analysis revealed that Fe content was lower in HC seedlings with high rustiness. The growth of 2-years-old ginseng was different following these varying seedling cultivation methods, but seedlings from NS were not different from those grown in SSN. Conclusions: For the propagation of 2-year-old ginseng plants, NS seedlings may be a good substitute for SSN seedlings.
Twin-bud seedlings and four-leaflet seedlings of ginseng were found and transplanted to field and their growth characteristics were investigated. 1. Appearance frequencies of twin-bud and four-leaflet seedlings were 0.3 and 0.4 percent, respectively, in common nursery bed: and were 6.6 and 28.4 percent, respectively, in polystem line. 2. Generally, the growth of twin.bud and four-leaflet seedlings were better than those of common seedlings both in aerial part and in root. Root weights of both type seedlings exceeded the common ones by 66 and 38 percent, respectively. 3. When they became two-year-old plants, leafiet number of common plant was 11.6, and those of twin-bud and four-leaflet-seedling plants were 18.1 and 13.8, respectively. There were no inflorescence in twin-bud-seedling plant, but the ratios of in florescent Plant in four-leaflet.seedling and common plant were 44.0 and 12.5 percent, respectively. 4. In two-year-old plant, root weights of twin-bud and four-leaflet-seedling plants were heavier than those of common ones by 27 and 20 percent, respectively.
This study was carried out to investigate the growth responses of Pinus densiflora seedlings to enhanced UV-B environment for 16 weeks in the field condition. The seedlings were treated with one of three levels of UV-B dosages - ambient UV-B, ambient + 3.2, and ambient + 5.2 KJ m$^{-2}$day$^{-1}$ and the irradiation was performed at the stage before the germination, the fully expanded cotyledon, and the primary needles grown more than 0.8cm in length of the seedlings, respectively. Enhanced UV-B irradiation reduced the height and the root collar diameter growth, and dry mass production of the seedling, and T/R ratio was increased by the UV-B treatment. Difference in seedling growth was observed by difference in time of the UV-B treatment. Among the seedlings which were treated with ambient - 3.2 KJ m$^{-2}$day$^{-1}$, height and root collar diameter growth was relatively high in the seedling received the UV-B treatment at the stage before the germination. The lowest dry mass production was observed in the seedlings received the UV-B at stage of cotyledon both in two levels of enhanced UV-B treatment. Chlorophyll concentration was reduced by enhanced UV-B irradiation, and chlorophyll a/b ratio was increased by the UV-B treatment.
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