• Title/Summary/Keyword: seedling method

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A Simple Method for the Assessment of Fusarium Head Blight Resistance in Korean Wheat Seedlings Inoculated with Fusarium graminearum

  • Shin, Sanghyun;Kim, Kyeong-Hoon;Kang, Chon-Sik;Cho, Kwang-Min;Park, Chul Soo;Okagaki, Ron;Park, Jong-Chul
    • The Plant Pathology Journal
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    • v.30 no.1
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    • pp.25-32
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    • 2014
  • Fusarium head blight (FHB; scab) caused mainly by Fusarium graminearum is a devastating disease of wheat and barley around the world. FHB causes yield reductions and contamination of grain with trichothecene mycotoxins such as deoxynivalenol (DON) which are a major health concern for humans and animals. The objective of this research was to develop an easy seed or seedling inoculation assay, and to compare these assays with whole plant resistance of twenty-nine Korean winter wheat cultivars to FHB. The clip-dipping assay consists of cutting off the coleoptiles apex, dipping the coleoptiles apex in conidial suspension, covering in plastic bag for 3 days, and measuring the lengths of lesions 7 days after inoculation. There were significant cultivar differences after inoculation with F. graminearum in seedling relative to the controls. Correlation coefficients between the lesion lengths of clip-dipping inoculation and FHB Type II resistance from adult plants were significant (r=0.45; P<0.05). Results from two other seedling inoculation methods, spraying and pin-point inoculation, were not correlated with adult FHB resistance. Single linear correlation was not significant between seed germination assays (soaking and soak-dry) and FHB resistance (Type I and Type II), respectively. These results showed that clip-dipping inoculation method using F. graminearum may offer a real possibility of simple, rapid, and reliable for the early screening of FHB resistance in wheat.

Effect of the Hopper of the Plug Transplanter and Moisture Content, Compaction Method, and Initial Irrigation of the Soil on the Seedling Survival rate at Transplant of Plug Seedling (플러그묘 정식시 정식기의 호퍼 크기, 토양수분, 초기관수량 및 진압방법이 작물에 미치는 영향)

  • 문성동;민영봉;박중춘
    • Journal of Bio-Environment Control
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    • v.6 no.4
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    • pp.258-263
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    • 1997
  • The result was summarized of basic test and field test to find what quantity of irrigation, what state of compaction and what size of transplanting hopper could induce the optimum taking root in the transplantation of plug seedling by transplanters, and thus acquired the basic data for the development of the related transplanters and the optimum growth and development control. Livability of vegetables after transplanting had no significant difference with respect to for the size of hopper, but was greatly affected by the length of seedlings. The longest possible length of seedling for transplanting and optimal length were found to be 30cm and 28cm, respectively. For irrigation when transplanting red pepper or Chinese cabbage it was thought that large-sized hopper was appropriate. The livability of plug seedling 10 days after transplantation was mainly affected by soil moisture content. Consequently it was thought no irrigation would be needed when transplanting at the soil moisture content of more than 18% ; irrigation of more than 50cc would be needed at the soil moisture content of 13% : initial irrigation of more than 100cc and subsequent irrigation would be needed at the soil moisture content of less than 3.8%. The improvement of soil compaction method (left and right side compaction) with conventional semi-automatic transplanter was not necessary, since there was no difference in livability depending on the compaction methods, left-right side compaction or back-forth-left-right side compaction.

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Vegetation Structure of Lower Stratum and Pinus densiflora Natural Regeneration Features from Micro-topography Classification in Pinus densiflora Forest of Anmyeon-do Island (안면도 소나무림 내 미세지형구분을 통한 하층식생구조와 소나무 천연갱신 양상)

  • Byeon, Seong Yeob;Kim, Hyun Seop;Yun, Chung Weon
    • Journal of Korean Society of Forest Science
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    • v.108 no.2
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    • pp.189-199
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    • 2019
  • The forest management paradigm has recently shifted from focusing on commercial production to focusing on ecosystem management. Accordingly, a natural seedling regeneration method that has a naturally high affinity has attracted much attention in recent years. The aim of this study was to determine the relationship among various environmental factors, lower stratum vegetation, and seedling regeneration in Pinus densiflora forests. The survey site comprised 50 sectors divided using the line transect method, and the survey data were divided into those from wet habitat (19 sites) and dry habitat (31 sites), depending on the soil humidity, and were analyzed separately to show the close relationship between soil humidity and natural seedling regeneration. As a result, the dry habitat exhibited high seedling density (157,419 trees/ha), with the main species being Quercus serrata, Zanthoxylum piperitum, Smilax china, and Pueraria lobata, while wet habitat exhibited low seedling density (57,895 trees/ha), with the main species being Stephanandra incisa, Castanea crenata, Lespedeza maximowiczii, Lysimachia barystachys, Aralia elata, and Styrax japonicus. The height and root-collar diameter under wet conditions exhibited faster growth than those under dry conditions. Height growth by the root-collar diameter in dry habitat increased faster than that in wet habitat. It was also confirmed that seedling regeneration in wet habitat exhibited a rapid growth pattern 5 years after germination. These results suggest that the seedlings begin to grow more rapidly after a period of suppression by competition with surrounding plants. Considering an ecosystem or ecological management approach, specific practices, such as bush control and vine clearing in wet habitats, should be more intensively conducted, especially at the beginning of the management operations.

Effect of Silicate on Bokto Direct Seeding Technology in Rice (규산질비료 이용 벼 복토직파기술개선 연구)

  • Park, Kwang-Ho;Kang, Yun-Kyu;Ahn, Duck-Hyun
    • Journal of Practical Agriculture & Fisheries Research
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    • v.7 no.1
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    • pp.35-46
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    • 2005
  • This research was conducted to improve and establish Bokto direct seeding technology use of silicate in rice. Bokto direct seeding technology has recently released to alternative new method for transplanting method of the rice plant. In recent rice production has serious problem due to higher cost and labor shortage in Korea. In particular rice has been faced on open market to internationally due to WTO, FTA agreement etc. Thus rice growers in Korea have been more concentrated on cost reduction using by direct seeding technology such as dry seeding and water seeding while those methods have severe constraints like poor seedling, more weed occurrence, lodging and poor grain quality. In recent a new technology for improvement of these problem has released to farmer's field and proven to alternative rice growing technology of transplanting. In this technology there was additional application of silicate which has known as soil improvement and this research was obtained in the following results. At the application of silicate, sand, and soil there was high in seedling establishment by 90~100% in pot experiment but there was 55.0% seedling stand of the untreated control. Under dry condition seedling stand was 0% at the untreated control while 65~100% high in application of silicate, sand, and soil. Meanwhile, under rainy condition untreated control there was 85% of seedling stand but soil application was 20% of seedling establishment due to blocking of oxygen and physical soil blockage.

Rice Seedling Establishment for Machine Transplanting VI. Effect of Mulching Materials on Raising Rice Seedling at Tray for Machine Transplanting (수도 기계이앙 육묘에 관한 연구 Ⅵ.제6보 상자육묘시 피복자재이용 효과)

  • Yun, Yong-Dae;Yang, Won-Ha;Kwang, Yong-Ho;Park, Seok-Hong;Park, Rae-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.1
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    • pp.9-15
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    • 1986
  • To establish an efficient light control method using three covering materials on tunnel shaped rice seed-bed at greening stage after seedling emergence, four rice cultivars, Nampungbyeo, Taebaegbyeo, Seonambyeo, and Seomjinbyeo were sown on 15 April and 10 May in 1983 and 1984 respectively. After seedling emergence by a simplified emerging methods the seedling boxes were moved onto tunnel shaped seed-bed which was covered with combined matrials of PE film, silverpoly sheet, and spunbonded polyester fabric. For machine transplanting of rice seedlings in cases of early season and optimum season seeding in central part of Korea, PE film tunnel with silverpoly mulched, and PE film tunnel methods with spunbonded polyester fabric mulched reduced injuries of non-parasitic seedling damping-off and a albinism as affected by it, protected rice seedlings from injuries by extremely low temperature in the night, and reduced less differences in diurnal temperature than those in the other covering methods. At late season seeding for double cropping system of paddy field in southern part of Korea, a single silver-poly or a single spunbonded polyester fabric-covered tunnel method showed good green seedlings, and prevent-ed extreme rising of diurnal temperature by light interception in the tunnel.

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Estimation of Seedling Stands and Influence of Water Foxtail in Barley Broadcasted before Rice Harvest (벼 입모중(立毛中) 보리 산파재배시(散播栽培時) 적정 입모수(立毛數) 설정 및 둑새풀 발생의 영향)

  • Ju, Jung-Il;Lee, Hee-Bong;Shin, Chul-Woo
    • Korean Journal of Agricultural Science
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    • v.26 no.1
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    • pp.6-11
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    • 1999
  • This experiment was carried out to estimate the appropriate seedling stand and to find out the factors of good establishment and the reason of the reduction of yield by water foxtail. The seedling stands was improved by the ice straw mulching and treading by the tracks of combine. The effect of mulching was higher than the treading. The appropriate seedling stands for example yield were estimated about 507 plants per $m^2$ and its yield was 24kg/10a. The higher density of water foxtail, the less the grain yield; 21.8% by 17 to 22 water foxtail stands per $m^2$ and 11.0% by 6 to 11 plants.

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Effects of Plant Growth Regulators on Seed Germination and Seedling Growth of Mountain Mulberry Seeds (Morus bombycis Koidz) (식물생장조절물질이 산뽕나무 종자의 발아 및 유묘생장에 미치는 영향)

  • Song, Min-Jeong;Kim, Kyung-Hoon;Hur, Young-Jin
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.19 no.1
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    • pp.101-109
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    • 2016
  • This is the fundamental research on restoring damaged vegetation areas in the vicinity of DMZ using local native plant species. This research is aimed at identifying effective plant growth regulators (PGR) for seed germination and seedling growth of mountain mulberry, and developing effective methods for managing its germination and growth. Mountain mulberry seeds were collected from the regions in the DMZ vicinity, and tests with seven treatments using four PGRs including $GA_3$, kinetin, ABA and NAA, were conducted. The germination rate was calculated in two different ways of both in a growth chamber and in a greenhouse after seven days observation, and the growth characters such as leaf width/length, seedling width/length and fresh/dry weight, have been surveyed in a greenhouse for three months. Although in the growth chamber the highest germination rate was shown at a group with the kinetin treatment, it was not significantly different to that of the control group. Groups with ABA or NAA presented relatively low germination rates. As for the greenhouse test, the germination rates of all groups ranged 20~30% without significant difference each other, the reason of which might be due to low absorption by the effect of a soil drench method used in this study. The entire growth characters with the treatments of $2.15mg{\cdot}L^{-1}$ of kinetin and $10mg{\cdot}L^{-1}$ of $GA_3$ were significantly different to the control. NAA treatment only showed better growth of seedling width compared to the control. Consequently, the most effective PGRs for the germination and growth of mountain mulberry near the DMZ was kinetin. Further research on examining the most effective concentration of them was needed.

A Study on the Cultivation of Enteromorpha prolifera(Muller) J.Agardh, Chlorophyta in Korea (녹조식물 가시파래, Enteromorpha prolifera의 양식연구)

  • 윤장택;조용철;공용근
    • Journal of Aquaculture
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    • v.16 no.1
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    • pp.44-50
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    • 2003
  • This study aimed to develop the methods of growing Enteromorpha prolifera natural seedlings in its natural habitat and artificial indoor seedlings by inducing spore release. Likewise, the study examined the possibility of mass production by developing cultivation techniques with cultivating examination. The natural seedling of E.prolifera thrived in a sea area composed of sand and mud, which Is its natural habitat. Growing of this alga on the seedling frame 20 cm high from the bottom at the intertidal zone in summer and 40 cm high in fall was found to be very effective. However, enabling the best attachment rate for artificial indoor seedling requires inducing spore release after drying the mature thalli in a dark place fur about 12∼24 hours and setting seedling nets in a dark water tank (spore solution) for 24 hours. Breeding E.prolifera in a pole-system farm is best done in shallow sea areas with mud or mud and sand geological feature. However, floating-system lam is better for deep-sea areas with fast current. Ideal farming places are sea areas with plenty of nutritional salt and safe places that protect the lam facilities against billows. Furthermore, an exposure method on seawater surface to produce larger output should be used.

Effects of Kind of Pot for Raising of Seedling and Planting Method on Growth and Fruit Characteristics in Cultivation of Watermelon under Plastic Film House (플라스틱필름 하우스 수박 재배에서 육묘 포트 종류 및 정식 방법이 생육 및 과실 특성에 미치는 영향)

  • Bae, Jong-Hyang;Lee, Sang-Uk;Choi, Jun-Hyuk;Kang, Nam-Hee;Kim, Ho-Cheol;Eun, Jong-Seon
    • Journal of Bio-Environment Control
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    • v.19 no.2
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    • pp.82-87
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    • 2010
  • To investigate effects of kind of pot for raising of seedling (single plastic pot, double plastic pot, circular peat pot, square peat pot) and planting method of the pot (3/3, 2/3, and 1/3 by the buried degree of pot height) on watermelon (Citrullus lanatus) growth, this research was conducted under plastic film house. Since planting of seedlings, number, area and photosynthesis rate of leaves, plant height and weight were superior in seedling grown in double plastic pot than those grown in the others. In fruit characteristics harvested at 12 weeks after planting, fruit height, diameter and weight were longer in double plastic pot than in the others, also fruit peel thickness and hilum diameter were thicker and shorter. Initial growth of the plant after planting with seedling grown in double plastic pot was superior in method buried the 2/3 degree of the pot height than the other methods.

Effect of Different Bottom Seats in Seed Tray on Seedling Growth of Rice (벼 육묘상자 깔판종류가 묘생육에 미치는 영향)

  • Jung, Byung Won;Sohn, Jae Keun
    • Current Research on Agriculture and Life Sciences
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    • v.19
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    • pp.23-29
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    • 2001
  • This study was carried out to develop a seedling raising method without nursery soils in automatic raising facility of rice seedlings for machine transplanting. Pre-germinated seeds were sowed on the seed trays with three different seats such as paper seat, corrugated board, and polyethylene film instead of nursery soils. The growth characteristics of the seedlings for 20 days after sowing were compared with that of a conventional standard raising method with nursery soils. There was no difference in plant height of 10-day old seedlings among the different nursery methods, but the plant height of seedlings grown for 15 days after sowing in the trays with fivefold newspaper and nursery soils was significantly taller than those on other nursery conditions. Dry weight of seedlings grown in the tray with fivefold newspaper bottom was heavier than those in nursery trays with a corrugated board and a piece of polyethylene film. The seedlings grown in a nursery tray with fivefold newspaper showed better seedling quality and root-mat formation compared with the other nursery trays. This result suggests that the healthy seedlings for machine transplanting can be raised in the paper-sheet tray without nursery soils. The labour hours and cost required for the soil-free method using paper was reduced by about 49.3% and 26.6%, respectively, compared with the semi-adult seedling on nursery soils.

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