• Weed & Turfgrass Science
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• v.3 no.1
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• pp.19-28
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• 2014

Research was initiated in greenhouse to investigate effects of polymer, calcium, perlite and chitosan on the growth of Kentucky bluegrass (KB). A total of 24 treatment combinations were used in the study. Treatments were made of water-swelling polymer (WSP), calcium, perlite, and chitosan in soil organic amendment (SOA). Significant differences were observed in germination rate, turfgrass coverage, turfgrass density and top growth among treatments. Germination rate, density and plant height varied with time after seeding. A proper mix of WSP is considered to be lower than 3% for turfgrass coverage and density. Regarding survival capability and top growth, however, it was good under 6%. Overall KB growth was more influenced by calcium and perlite than chitosan. Calcium and perlite were the most effective elements for early survival capacity and turfgrass density, respectively. But no effect was found by chitosan. Top growth increased with three elements, being perlite > calcium > chitosan. The chitosan was effective in early germination, but there was no effect on top growth until 3 weeks, when compared with others. A further study is needed for investigating the effect of these materials on the growth characteristics in mixtures of sand and SOA before a field application.

• Journal of Korea Water Resources Association
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• v.43 no.2
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• pp.153-165
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• 2010

Large-Scale Particle Image Velocimetry (LSPIV) is an extension of particle image velocimetry (PIV) for measurement of flows spanning large areas in laboratory or field conditions. LSPIV is composed of six elements - seeding, illumination, recording, image transformation, image processing, postprocessing - based on PIV. Possible error elements at each step of Mobile LSPIV (MLSPIV), which is a mobile version of LSPIV, in field applications are identified and summarized the effect of the errors which were quantified in the previous studies. The total number of elemental errors is 27, and five error sources were evaluated previously, seven elemental errors are not effective to the current MLSPIV system. Among 15 elemental errors, four errors - sampling time, image resolution, tracer, and wind - are investigated through an experiment at a laboratory to figure out how those errors affect to velocity calculation. The analysis to figure out the effect of the number of images used for image processing on the velocity calculation error shows that if over 50 images or more are used, the error due to it goes below 1 %. The effect of the image resolution on velocity calculation was investigated through various image resolution using digital camera. Low resolution image set made 3 % of velocity calculation error comparing with high resolution image set as a reference. For the effect of tracers and wind, the wind effect on tracer is decreasing remarkably with increasing the flume bulk velocity. To minimize the velocity evaluation error due to wind, tracers with high specific gravity is favorable.

• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.215-220
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• 2007

This study was conducted to examine the establishment of bovine ES-like cells having pluripotency. The hatched blastocysts derived from culture of in vitro fertilized embryos for 10 to 12 days dissociated mechanically into ICM-and trophectoderm-rich clumps using needle, and cultured onto mitotically-inactivated MEF feeder layer. The primary colonies originated from ICM cells were detached mechanically 7 days after seeding and subsequent subculture was conducted at intervals of every 5 to 7 days. Two ES -like cell lines were established and maintained over 40 passages. Self-renewal of the established lines was confirmed by examining the alkaline phosphatase activity, stem cell-specific marker profiles including SSEA isotopes, Oct-4 and STAT3. Moreover, the established cell lines could produce anchorage-independent embryoid bodies (EBs) with gradual decrease of Oct-4 transcript level in time-dependent manner.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.1
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• pp.16-21
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• 2005

This study was conducted to establish the elaborate nitrogen fertilization method to enhance N use efficiency in direct-seeded rice on flooded paddy. The nitrogen uptake by rice plants was insignificant until 25 days after seeding, and increased gradually thereafter. During this early growth stage, rice plants absorbed only the $4\%$ of basal applied N, while the $45\%$ of N fertilizer remained in the paddy soil. The absorption of basal N by rice plants was almost completed at 46 days after application. Nitrogen top-dressed at 5-leaf stage was well matched to crop nutrient demand, so it could be absorbed so actively in 8days after application. As a result, we could cut down the amount of N fertilizer to $36\%$ of the basal N level without significant difference in yield. Plant recoveries of fertilizer $^{15}N$ applied with different application timings were $7.8\%$ for basal, $9.4\%$ for 5-leaf stage, $17.1\%$ for tillering stage, and $23.4\%$ for panicle initiation stage, respectively. When urea was applied with nitrogen fertilization practice based on basal incorporation (BN), plant recovery of $^{15}N$ at harvest was $31.0\%$, which was originated from $13.7\%$ for grain, and $21.3\%$ of the fertilizer $^{15}N$ remained in the soil, and the rest could be uncounted. Plant recovery of fertilizer $^{15}N$ applied with nitrogen fertilization practice based on topdressing at 5-leaf stage (TN), where N rate was reduced by $18\%$ compared with BN, was $35.1\%$ (grain $15.6\%$), and $19.9\%$ of the fertilizer $^{15}N$ remained in the soil, and the rest could be uncounted. TN showed a higher $^{15}N$ recovery than BN because it was to apply N fertilizer at a time to well meet the demand of rice plant direct-seeded on flooded paddy. We concluded that TN would be the nitrogen fertilization method to enhance N use efficiency in direct-seeded rice on flooded paddy.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.15
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• pp.69-75
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• 1974

This experiment was conducted to investigate the influence of different amount of nitrogen and deficiency of soil moisture on yield components of soybean. Soybean were seeded on 1/2000a wagner pot. Deficiency of soil moisture was treated at each growth atage of soybean. 1.In case of deficiencyt of soil moisture at the flowering time in the plot of non-nitrogen(NO D3), the growth duration of soybean was shortened about three to four days. 2. The leaf area was greatly affected by the influence of both treatments till 49days after germinating. 3.The increase of stem height, stem doameter,number of branches and lengeh of the branches came to an end about 70 days after seeding. These growing condition of tje soybean were lowest the plot of No D$_1$,in which the frowth of the soybeans were poor at the early stage. 4.The number of pods was not increased by the increase of fertilizing nitrogenous fertilizer. The number of pods was much decreased by the influence of soil mousture deiciency, and under this condition, the proportion of main stem pods and two or three grain pods was high. 5.The 3rd and 4h nodes and the 10th to 12th nodes from bottom had more pods than the other nodes had, but of the plants had grown well, they had more pods on the 3rd and 4th nodes, but if the plants had grown poorly, they had more pods on the 10th to 12th nodes. 6.The content of protein in the soybean was low at the plit of N。D$_4$which had not heavy weight of 100 grains, and the content of oil in the soybean was low in the plot in which each plant had a small number of grains.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.1
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• pp.53-61
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• 2020

Cell migration is a central process for recovering from wounds triggered by physical distress besides embryogenesis and cancer metastasis. Wound healing assay is widely used as a fundamental research technique for investigation of two-dimensional cell migration in vitro. The most common approach for imitating physical wound in vitro is mechanical scratching on the surface of the confluent monolayer by using sharp materials. The iron metal pin with a suspension spring for fine adjustment of the orthogonal contact surface between the scratching point and the individual bottom of multi-well plate with planar curvatures were adopted for the creative invention of a 96-well plate wound maker. While classic tips drew diverse and zigzag scratching patterns on the confluent monolayer, our wound maker displayed synchronized linear wounds in the middle of each well of a 96-well plate that was seeded with several cell lines. Given that several types of multi-well plates commercially available are compatible with our lab-made wound maker for creating uniform scratches on the confluent monolayer for the collective cell migration in wound healing assay, it is certain that the application of this wound maker to the real-time wound healing assay in high content screening (HCS) is superior than utilization of typical polypropylene pipette tips.

• Journal of the Economic Geographical Society of Korea
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• v.11 no.2
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• pp.216-232
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• 2008

This paper aim to review the extent of Park Je-Ga's geographical interest and knowledge and where he intended to make good use of them through Bukhakui(北學議). In particular, it was classified and interpreted as the contents related to agricultural, industrial and commercial geography focusing on the contents of the economic geography. As for the contents related to agricultural geography, it suggests minutely and precisely the necessity and concrete methodology for farmland, seeding, fertilization, breeding of cattle, sericulture and fruits. As for the contents related to industrial geography, it lays emphasis on the pursuit of the modernization of ours by means of accepting high-tech, industrial engineering and knowledge from Western scholars who were staying in Beijing. As for the contents related to commercial geography, it puts emphasis on the conversion of idle labor capacity of the nobility to practical production capacity, the pursuit of economic vitalization by opening land transportation through wheels and the trade by sea with neighboring countries such as Qing, Japan, Ryukyu, Vietnam and so on. It can be known through this study that Park Je-Ga was a realist who made an effort to raise the economic power of the region and the country with using his endeavor of economic geographical interest and knowledge. His economic geographical interest and knowledge were connected directly with practical use. If his economic geographical knowledge and way of thinking had been accepted successfully at that time, the economic power in the latter half of the Joseon dynasty could have leaped to a considerable degree.

• Journal of the Korean Society for Marine Environment & Energy
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• v.6 no.2
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• pp.3-15
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• 2003

Recently more than 450 incidents of oil spill a year have occurred in nearshore of Korea, which caused unmeasurelable losses in fisheries and severe damage in marine ecosystem. Two approaches remain paramount in any response to marine oil spill : the enhancement of natural dispersion of the oil by using dispersants, and mechanical recovery using booms and skimmers. A technique currently receiving fresh attention is the enhancement of the natural bioremediation of oil through the application of micro-organisms and/or nutrient. Oil, like many natural substances, will biodegrade over a period of time into simple compounds such as carbon dioxide, water and biomass. Bioremediation is the term used to describe a range of processes which can be used to accelerate natural biodegradation. More specifically biostimulation is the application of nutrients, and bioaugremetation or seeding is the addition of microbes specially selected to degrade oil. Bioremediation is an economically attractive method for the clean-up of oil-contaminated area. Bioremediation has been demonstrated to be an effective oil spill countermeasure for use in cobble, sand beach, salt marsh, and mud flat environment.

• Korean Journal of Organic Agriculture
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• v.7 no.1
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• pp.35-48
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• 1998

The total amount of dry matter for the green manure crops was great wheat> rye> barley> Italian ryegrass in that order. The green manure crope were verified to have the reducing effect of injury of successive croppingin peper, mainly reducing the occurance of the most important pepper disease, Phytophthora capsisi, and enhancing the pepper quality in the fruit length and diameter. The direct seeding using current commercial pepper variety was proved as not economical one. In the first year of compost application, the growth and yield of red pepper were rather somewhat decreasing compared with those of check plot applied with organic fertilizers. compst application increased the content of organic matter in soil, which suggested compost could be applied for the sustainable purposes. In preference analysis about taking the new technique, the smaller farmer's cultivation area the more they wanted to accept the LISA farming, compared with the conventional one, could be possible to save 12% in the inorganic fertilizer expenses but wasted 412% ant 163% in both organic fertilizer and operator labor expenses, respectively. At the same time, the LISA decreased 15% in production cost but increased 225% and 139% in organic fertilizer quantity and operator labor hours. Since there was a great deal of difference in technological and economic factors from two farming methods, LISA multi-goal decision modeling is further required.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.3
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• pp.147-154
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• 2021

Cutting management has been identified as a critical factor in the alfalfa production systems because it has a significant impact on maximizing yield and maintaining the forage quality. The objective of this experiment was to determine the proper cutting height according to harvesting time for optimizing nutrient yield and forage nutritive quality of alfalfa grown in alpine regions of Korea. Alfalfa was sown at a seeding rate of 30 kg/ha in August 2018 and harvested at four cuttings in 2019 (3 May, 2 July, 11 September, and 13 October). Cutting heights were adjusted at 5, 15, and 25 cm above the soil surface. Alfalfa plant was tallest at the third cutting (109 cm), which was on average 35 cm taller than the first or second cutting. Relative feed value (RFV) remained unaffected by cutting height at the first harvest, but increased consistently in subsequent harvests as cutting height increased. Alfalfa collected at the first and fourth cuttings had the highest RFV (mean 152), which was on average 8 and 67 units higher than the second and third harvests, respectively. At each harvest, in vitro dry matter digestibility was highest in alfalfa cut at a 25-cm height. Dry matter (DM) production at each cutting height was highest in the first cutting, accounting for on average 36-37% of total annual DM production, and lowest in the fourth harvest, accounting for about 11-13% of the total DM yield. The total dry matter production (in four harvests) was 4,218 kg/ha higher when alfalfa was subjected to a cutting height of 5 cm rather than 25 cm. Cutting height had no effect on total crude protein yield, but from the first to fourth cutting, the protein yield followed a decreasing trend. Finally, there were visible declines in forage nutritive quality when alfalfa was cut at a shorter height. However, the magnitude of difference in total forage yield may outweigh the slight decline in forage quality when alfalfa is cut at a lower height. The findings of this study could help the alfalfa growers make better harvest management decisions.