• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.197-210
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• 2009

To improve the forage quality and reduce nitrogen input, trial was conducted on the effect of seeding method, combination, and nitrogen fertilizer with WCB (whole crop barley) and leguminous plant. Present experiment was carried out by split-split design having seeding methods for main plot, combinations for sub-plot, and nitrogen fertilizers for sub-sub plot with three replications. When WCB and leguminous plant were mixed-sown, WCB showed earlier heading and maturing than those of inter-sown, and the more nitrogen delayed growth stage a little. Occurrence of BaYMV (Barley Yellow Mosaic Virus), a serious disease caused by soil fungi and decrease barley yield, was deterred by mixed-seeding as compared to inter-sown barley a little. Inter-sown WCB increased the number of spike per $m^2$ as compared to mixed-seeding showing more spikes with nitrogen increase. WCB produced much fresh and dry matter yield at mixed-seeding than inter-seeding, and had advantage with hairy vetch (HV). Increased nitrogen showed much forage yield, however, half application of it is considerable for environmental-friendly farming. Electric conductivity (EC) decreased in inter-cropping or mixed-sowing soil with WCB and leguminous crop after harvest. But, organic matter (OM) content of soil after harvesting was vice versa. Acid detergent fiber (ADF) and Neutral detergent fiber (NDF) of WCB plant were higher at mixed-seeding than those of inter-sown ones. It showed increased tendency with time progress.

• Journal of Korean Society of Forest Science
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• v.81 no.1
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• pp.53-65
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• 1992

For elucidating effective methods of restoration of forest recreational sites where management goals are maintaining naturalness and conserving natural ecosystem, seeding, fertilization and soil surface treatment were used for four years at the devastated forest-floor. For restoration of forest-floor vegetation, factorial experiment was used with a split plot design(main plot : fertilization, subplot : soil surface${\times}$seeding) and a randomized complete block design (fertilization${\times}$seeding) at the Kwanaksan Aboretum, Anyang, Kyonggido. Results were summarized as follows : Soil surface softening with tipping and ripping and straw-mat mulching (70% coverage) treatment was effective on germination, survival and growth of seeded vegetation at devastated forest-floor. Especially, straw-mat mulching treatment was effective on soil surface stabilization and seedling's survival at eroded soil surface, while complete soil surface softening treatment was effective on germination, survival and early growth of tree species of late-successional series. Introducing seeds of native species of pioneer or early-successional series, with good growth capability in barren soil was effective on rapid restoration in devastated forest-floor with its soil surface previously compacted and its surviving seeds washed away. When the seeding and straw-mat mulching after partial soil surface softening with tipping and ripping treatment were employed, it took about three years to restore the devastated forest-floor where surface erosion had been undertaken for an extended period of time and where naturally surviving seeds of native species had been washed away. Softening treatment of soil surface was effective for about two years, and seeding and soil surface treatment increased number of seedlings and improved soil surface environment through fixing of movement of the fallen leaves. Fertilizing effect was not oberserved, mainly due to seeding exposure and poor physical condition including soil surface erosion, low soil water potential and drought, etc, at the field experimental site. However, application of nitrogen and phosphate fertilizers was effective on seedling survival of the species in late-successional series, while lime application adversely affected the seedling survival.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.2
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• pp.151-159
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• 2012

Direct seeding of rice is a time- and laborsaving method, compared to machine transplanting. However, the area planted to direct-seeded rice is decreasing because of instability of seedling establishment, lodging, occurrence of weedy rice, and deterioration of grain quality. A newly developed wet-hill-seeding of rice, a type of direct seeding method, has shown features of growth similar to machine transplanted rice. This study was carried out to compare tillering pattern, heading characteristics and grain filling traits between transplanting and wet-hill-seeding in rice. Tillering of wet-hill-seeded rice was more vigorous than that of transplanted rice showing maximum tiller number 30~100% higher than transplanted rice. Wet-hill seeded rice showed later heading and shorter heading duration than transplanted rice. To find out the relationship between heading characteristics and grain filling traits, every panicle was tagged for flowering date, and the panicles on each heading date were inspected for grain filling traits. Wet-hill seeded rice and transplanted rice exhibited no significant difference in culm length, panicle length, the percentage of grain filling and perfect brown rice on each heading date during total heading period. Therefore, we conclude that wet-hill-seeding method is not inferior to machine transplanting in terms of seedling establishment, growth, grain filling and head rice yield.

• Korean Journal of Agricultural Science
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• v.45 no.4
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• pp.615-622
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• 2018

This study was done to determine the optimum sowing time for spring sowing wheat in the southern region of the entire Korean peninsula. Jokyoung and Keumkangmil were sown four times at one-week intervals starting from Feb. 14, 2013. The thousand grain weights of the two wheat varieties were the highest on February 14 with the seed weights ranging from 36.6 to 40 g and significantly decreasing as the seeding time was delayed. The unmatured grain percentage increased sharply when both cultivars were sown starting on March 7. The grain yields were the highest for Keumkangmil with 3.07 MT/ha when sowed on February 21 and 3.37 MT/ha for Jokyoung when sowed on February 14. In both cultivars, the grain yield decreased drastically when they were sown on March 7. Ash content did increase when the sowing date was delayed. The flour gluten index was the highest at 96.2 in Keumkangmil with a February 28 sowing, and the sodium dodecyl sulphate (SDS) segmentation was the lowest ranging from 63.8 to 65.3 mL with the February 28 sowing. The falling number tended to increase with the delay of the sowing period. The flour milling rate was gradually decreased with the delay of sowing, and the bran gradually decreased. When both cultivars were sown after February 28, the grain and flour yields sharply decreased. In the southern region, the optimum time for the spring sowing of wheat is from February 14 to February 21.

• Korean Journal of Agricultural Science
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• v.2 no.2
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• pp.331-339
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• 1975

This experiment was carried out to obtain some imformations about the ecological variations of the heading time of the newly breeded Indica x Japonica varieties/pedigrees according to the seasonal changes of the seeding date. day length and temperature. The results obtained are summarized as follows; 1) At all varieties. days to heading showed a straight reduction according as the seeding time was delayed. 2) At the group of Japonica varieties. the days to heading of early maturing varieties were much shortened and that of medium or late maturing varieties were little or not shortened under high temperature condition but at the group of Indica ${\times}$ Japonica va rieties/pedigrees. days to heading were significantly elongated under high temerature condition. 3) At the group of Japonica varieties. the effect of the short day condition on the days to heading was significantly high at the medium and the late maturing varieties than at the early maturing varieties. however. the short day effect on the reduction of days to heading was not related with the earlyness of each varieties at the group of Indica ${\times}$ Japonica varieties/pedigrees. 4) Days to heading of all varieties under the high temperature condition were longer than that under the ordinary culture. On the other hand, the days to heading of Japonica varieties under the short day treatment were shorter than the days to heading of ordinary culture but at the group of Indica x Japonica varieties/pedigrees. the days to heading under the short day treatment were longer than ordinary culture. 5) At both varieties groups. the days to heading at each seeding time were significantly correlated with days to heading at ordinary cultivation. fl) At the group of Japonica varieties. relatively high correlation(p=0.1) was recognized between the days to heading by delay of seeding time, but at the group of Indica ${\times}$ Japonicava rieties/pedigrees, correlation between the ordinary heading date and the shortening rate of days to heading was very low.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.1
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• pp.9-16
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• 2019

This study was carried out to investigate the effects of seeding dates and cultivated period on the growth characteristics, dry matter yield and feed value of Teosinte new variety "Geukdong 6"[Zea mays L. subsp. mexicana (Schrad.) H. H. Iltis]for feed. The experimental design was arranged in a randomized block design with three replications. Treatments consisted of five time seeding dates, 10 May(T1), 25 May(T2), 10 June(T3), 25 June(T4) and 10 July(T5), and same time harvesting, 22 October. Therefore, growing period were 164 days(T1), 149 days(T2), 134 days(T3), 119 days(T4) and 103 days(T5), respectively. In growth stage at harvest time, T1, T2, T3, T4 and T5 were early flowering, end heading, middle heading, early heading and early heading stage, respectively. Plant length and dead leaf were highest in T1, but leaf width and number of leaf were highest in T2 than others (p<0.05). Leaf length, stem diameter and number of tiller were not significantly different among the treatments (p<0.05). Stem hardness was higher in order of T1(2.0)> T2(1.9) > T3=T4(1.7) > T5($1.2kg/cm^2$). Fresh yield and dry matter yield showed significantly higher as the sowing time was faster and the cultivation period was longer (T1 > T2 > T3 > T4 > T5, p<0.05). Crude protein, crude fat and TDN content were highest in T5, but ADF and NDF content were highest in T1 than others (p<0.05). T1, T2 and T3 showed significantly higher crude protein yield compared to T4 and T5 (p<0.05). Total digestible nutrients yield were higher in order of T1 > T2 > T3 > T4 > T5 (p<0.05), and relative feed value were higher in order of T5 > T4 > T3 > T2 > T1 (p<0.05). Based on the above results, seeding dates could be recommended from the early May to the late May, and it is efficient that the cultivation period is over 150 days for dry matter yield, crude protein yield and total digestible nutrients yield.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.2
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• pp.90-97
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• 2005

This study aimed to address problems and suggest solutions in applying seed mixture design criterior of slope revegetation works according to the "Slope revegetation design and guidelines(proposed)" proposed by the Ministry of Construction and Transportation. To do this, a proper thickness of attaching soil media was identified in April experiment where the thickness and slopes varied. The results were as follows. In the test, the coverage rate of plot where exotic grass was not sowed increased with time and was 79.3% after three months of seeding, which indicated less risk of soil erosion. When applying the provisional standards of the plant mix proposed by the Ministry of Construction and Transportation, tree seeds and native plants were found ill in the early stage in all test lands due to exotic grass. This was because exotic grass which grew well in the early stage, could grow densely, so it might hamper the growth of other plants. When slope revegetation were planted and goals were set for herb, shrub and tree dominant mixture types, it was required to shift focus toward nursing up native plants first rather than early plantation of exotic grass.

• Journal of Embryo Transfer
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• v.26 no.2
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• pp.111-115
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• 2011

In general, zona pellucida (ZP) of the blastocyst has to be removed first, then either isolated the inner cell mass (ICM) or ZP-removed whole blastocyst, which is then cultured on the feeder layer to induce ICM outgrowth for the generation of embryonic stem cells (ESC). However, it is unclear whether ICM isolation before seeding on feeder layer is beneficial or not because the interaction between ICM and trophoblasts may affect cellular growth and/or pluripotency during the culture on the feeder. In the present study, two ZP removal methods (mechanically by splitting with a 28-gauge needle versus chemically by the treatment of acid-Tyrode's solution) and two ICM isolation methods (ZP-free whole blastocyst seeding versus mechanical isolation of ICM) were evaluated for the efficient isolation and culture of putative parthenogenetic bovine ESC. The number of maintained outgrown colonies was counted in each experimental group. As the result, mechanical removal of ZP with a needle and followed by whole ZP-free blastocyst seeding on feeder cells tended to attach more on the feeder layer and resulted in more outgrown colonies with its simple and less time-costing benefits. Currently we are generating ESC lines in HanWoo cattle by using this method for initial outgrowth of the parthenogenetic bovine blastocysts.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.132-132
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• 2022

The application of direct seeding cultivation reduces time, labor, and cost. However, this application often has poor seedling establishment and leads to lower yield as compared to transplanting system. The tolerance to anaerobic and low temperature germination is important to improve seedling establishment and the wide-spread application of direct seeding method. This study was carried out to evaluate the responses of three japonica cultivars to different temperatures (15℃, 18℃, 21℃, 24℃, and 27℃) and different flooding levels (1 cm, 4 cm, 7 cm, 10 cm) during germination and seedling development. The mean survival percentage significantly increased (P<0.05) when the flooding level decreased and when temperature increased. There were significant effects of the interaction between temperature and water depth on survival percentage and seedling height. When temperature decreased from 27℃ to 15℃, the germination duration significantly increased from 6.4 days to 16.3 days while the germination speed, survival percentage, and seedling height decreased from 5.3 seeds day^-1, 61.9% and 190.6 cm to 2.2 seeds day^-1, 33.2%, and 47 cm, respectively. The increase in temperature under submergence condition was associated with the increased expression of Amy3D and ALDH2a but the decreased expression of ADH1 and PDC1. The results of this research would be used for further studies and breeding programs to improve rice seedling establishment and the application of direct seeding cultivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.282-292
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• 2015

Seeds of colored rice generally have high anthocyanin and flavonoid content but some cultivars have low seed germination rate. This defect poses unstable seedling establishment in nursery bed. The seed gravity variations of 10 colored rice varieties and its effect on germination, seed soaking duration and their optimum seeding rates for seedling raising in machine transplanting were investigated in the experiment. Based on seed gravity distribution of the 7 black- and 3 red-colored rice varieties at three levels of seed gravity (1.0<, 1.0~1.06, 1.06>), the black colored seeds such as Josaengheugchal and Sinmyungheugchal had high amount of specific gravity of below 1.0 ranging from 86~ 96%, while they had only 3~13% of seeds in specific gravity above 1.06. Sintoheugmi, Heugjinju, Heugnam, Heugkwang had various mass of seeds, showing 29~44% for specific gravity of below 1.0, 24~39% for 1.0~1.06 and 25~45% for above 1.06. On the other hand, the red colored rice such as Hongjinju, Jeogjinju and Geonkanghongmi had high percentage of specific gravity of above 1.06 with 84~86% while they had only 9~12% of specific gravity below 1.0 similar to Ilmibyeo of noncolored rice. The black colored seed generally showed low germination percentage, slow germination speed and long mean germination time, and low water absorption rate as compared with seed of the red colored rice which was similar to those of Ilmibyeo used as a control cultivar. The black colored seeds took 2~4 days longer seed soaking duration than the red colored rice before germination. This was related to high seed amount of specific gravity below 1.0 in black colored rices. The high amount of seed gravity above 1.06 in the colored rice seeds was positively correlated with percentage of germination, germination speed and mean germination time, ripened grain ratio and water absorption. Seed gravity distribution effect were less pronounced between back- and red- colored seeds in seedling emergence. The black colored rice has slightly lower normal seedling emergence rate than the red colored rice and Ilmibyeo in the seedbed soil due to high percentage of ungerminated seed. Normal seedling emergence rate of the black colored rice in the seedbed was 75.2~82.2% for 10-day old seedling and 85.3~ 90.9% for 30-day old seedling which was lower by 4.5~ 8.0% and 0~3.3%, respectively, than the red colored rice. Based on the normal seedling number per tray of Ilmibyeo for seeding rate of 10-day and 30-day old seedlings, the recommended seeding rate of black colored rice is 200~ 220g seeds for the 10-day old seedling and 110~130g for the 30-day old seedling in transplanting rice while the seeding rate of the red colored rice was 220g seeds for the 10-day old seedling and 130g for the 30-day old seedling.