• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.3
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• pp.317-322
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• 1997

In order to investigate vigor of rice seed pregerminated, some organic and inorganic component content, response of ecotypes, and effect of gibberellin treatment, under different levels of hastening of germination, were examined. Protein content was high in dried seed, the larger size in length of pregerminated seed, the less protein content, but lipid content was a reverse tendency to protein one. Potassium content was low in dried seed, the larger size in length of pregerminated seed, the more potassium content, but increase dramatically at above 10 mm in length of sprout. Magnesium content showed a same tendency to potassium one. The ability of regermination was high in early maturing cultivar, but almost lost in the pregerminated seeds with above 5 mm in length of sprout. The regermination percentage of dried seed was lower than that of pregerminated seed with 0.1∼0.5 mm in length, but was similar to that of pregerminated one with 1∼3 mm in length of sprout. The effect of gibberellin on regermination percentage was highest at 10 ppm and showed high with small sized one of pregerminated seed. The effect of gibberellin on plant height, fresh weight, and dry weight showed a same tendency to regermination percentage.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.528-534
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• 2009

The study of germination and seedling growth characteristics of Neem under different soil and environment conditions was undertaken. The seed germination started 8 days after sowing in commercial bed soil, whereas, delayed germination was observed in sandy-loam (15 days) and sandy (19 days) soil. The highest germination (73.33%) was observed in commercial bed soil in green house, whereas, the lowest germination was observed in sandy soil (16.67%) and sandy-loam soil (8.33%). The seeds in the open field (sandy soil) also showed poor (10%) germination. The mean number of germination seed/day (GD) and seed germination vigor rate (GV) both were highest in the commercial bed soil with 0.733% and 16.67% respectively in the green house, whereas sandy and sandy-loam soil in green house and open field (sandy soil) all showed much lower GD and GV values. The seedling characteristics of nursery revealed that the seedling grown in the growth chamber in commercial bed soil was significantly higher in all the parameters comparing to others grown in green house and open field. The growth was nearly 7 fold in the chamber compared to that of the green house nursery observed in three months old seedlings. Likewise, HPLC analysis revealed that the green house grown seedling contain higher quantity of pigments compare to the chamber grown seedlings. Among the soils used the commercial soil alone or in combination with sandy and sandy-loam soil in the ratio of 2:1:1 respectively with the temperature of $27{\pm}2^{\circ}C$ showed better for Neem nursery preparation.

• Journal of Forest and Environmental Science
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• v.35 no.4
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• pp.223-231
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• 2019

Castanopsis indica (Sil Batna) is an ecologically valuable multipurpose indigenous tree species of Bangladesh. Considering its high value but poor natural regeneration due to seed dormancy, the authors conducted an experiment at the Institute of Forestry and Environmental Sciences Chittagong University (IFESCU) during 2012-2013 to find out effective pre-sowing treatments. Ten pre-sowing seed treatments were applied on mature, even-sized seeds namely-control; sandpaper rubbing; nail clipping; immersion in normal water (at room temperature: 24℃) for 24 hrs., 48 hrs. and 72 hrs.; immersion in hot water (80℃) for 1 minute; soaking in 10% dilute H₂SO₄; soaking in 10% HCl for 5 minutes; and sowing in propagator house. Seeds sown after sandpaper rubbing at the distal end revealed best performances by providing highest germination percent (66.7%), germination energy (30%), germination index (0.17), germination rate (0.0145), germination value (30%) and plant percent (66.7%) within shortest period (38 days). The treatment also produced most vigor seedlings with 20.9 cm shoot height, 15 node number, and largest leaves (11.1 cm×2.9 cm). Hence, it is recommended to adopt sandpaper rubbing method for maximum germination and quality seedlings.

• Korean Journal of Plant Resources
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• v.36 no.6
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• pp.580-587
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• 2023

Androsace septentrionalis is a grass species restricted to North Korea; however, it is at the brink of extinction due to habitat loss and environmental changes caused by natural disasters and anthropogenic activities. This study was conducted to characterize the dormancy conditions of A. septentrionalis in an effort to conserve this North Korean plant resource. For this purpose, the morphological characteristics and vigor of A. septentrionalis seeds were examined, and its germination characteristics under different temperature conditions (15/6℃ and 25/15℃ ), low-temperature stratification, and gibberellin (GA₃) treatment were determined. The results revealed that A. septentrionalis exhibits non-deep type morpho-physiological dormancy, and low-temperature stratification treatment was not effective in breaking the dormancy of A. septentrionalis seeds. Meanwhile, GA₃ treatment significantly increased the mean germination time, rate, and speed of the seeds. The optimal conditions for the germination of A. septentrionalis seeds were 25/15℃ fluctuating temperature and 500 mg·L¹ GA₃ treatment. The results of this study are useful for the mass propagation of A. septentrionalis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.2
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• pp.171-181
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• 2021

The purposes of this study were to determine how changes in temperature affect germination rates and growth of hulled and dehulled barley, malting barley, and naked oat plants, and to measure chlorophyll content, photosynthetic efficiency, and secondary compounds (total phenol, total flavonoid, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity) in plants grown at 13℃ or 25℃). Various types of barley seeds were collected from areas with ideal conditions for barley cultivation, hereinafter referred to as IA, and also from areas where barley cultivation is more difficult due to lower temperatures, hereinafter referred to as LTA. Seeds were tested for seed vigor. While there were significant differences in the electrical conductivity values between seeds collected from certain specific areas, no significant differences were evident between IA and LTA seeds, regardless of the type of barley seed. When plants were grown at 25℃, there were no significant differences in germination rates, plant height, root length and shoot fresh weight between plants originating from IA and LTA. However, there were differences in the measured parameters of some specific seeds. Similarly, under the low temperature condition of 13℃, no differences in the emergence rate, plant height, and shoot fresh weight were evident between plants originating from IA or LTA, regardless of the type of barley. However, there were differences between some specific seeds. One parameter that did vary significantly was the emergence date. Hulled barley and malting barley emerged 5 days after sowing, whereas naked oats emerged 7 days after sowing. There were no differences in the chlorophyll content and photosynthetic efficacy, regardless of the type of barley. There were no significant differences in total phenol, total flavonoid content, and DPPH radical scavenging activity between plants originating from IA and LTA, regardless of the type of barley. However, there were differences between some specific seeds. In particular, for malting barley the total flavonoid content differed in the order of Gangjin > Changwon > Haenam = Jeonju > Naju. The results indicate that crop growth, yield and content of secondary compounds in various types of barley may be affected by climate change.

• Journal of Korean Society of Forest Science
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• v.99 no.6
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• pp.849-854
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• 2010

This study was conducted to investigate the variation of seed and germination characteristics among populations and among individuals within populations of Abies koreana Wilson distributed in Korea. Cones was collected from 4 natural populations and their seven seed qualities as well as three germination behaviors was measured and tested. Statistical analysis showed that there were significant differences among populations as well as among individuals within populations in 8 traits except for percentage of sound seeds and seed vigor. Coefficients of variation in percentage of sound seeds, germination percentage and germination rate is relatively high (43.8~57.2%) compared to other traits (11.0~28.3%). The germination of seed started on the seventeen day after sowing and it was completed within seventy-nine days. The final seed germination capacity was found high except for Mt. Duckyu (8.5%) and varied from 39.7% to 47.7%. Mean germination time ranged between 38.1 and 43.6 days and germination rate varied from 1.98 to 1.39 ea./days. As a result of simple correlation analysis between seed characteristics and germination characteristics, the percentage of sound seeds showed a significant positive correlation with the germination percentage and the germination rate but showed a negative correlation with the mean germination time.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.1
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• pp.10-14
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• 2006

We tested the seed viability of Pinus rigida using accelerated aging to discover optimum times and temperatures far artificially accelerated aging. Seeds were artificially aged at different temperatures and during different tines. The seed viability was affected by the accelerated aging and by temperature with a decline in germination and seed vigor. The aging index of P. rigida seed was 0.31 at $35^{\circ}C$ and seed viability was nearly lost after aging treatment at $40^{\circ}C$ for 15 days. The optimum temperature of P. rigida far the aging test was decided to be approximately $37^{\circ}C$ on the basis of the aging index. Inorganic materials and conductivity of leaching solution from aging seeds increased with the increase of aging period. The accelerated aging test was considered to be a suitable method to evaluate the seed viability of tree species. Because seed characters are much different among tree species, however, more studies need to be done to discover the optimum conditions for aging by tree species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.1
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• pp.103-114
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• 1994

Pearl millet is a $C_4$ plant and summer crop originated from west Africa, and the sixth most important cereal in the world and the most widely cultivated millet in the semi-arid tropics as a major staple food crop. Its grain of higher quality protein is used to make unleavened bread chapatis and prepared as gruel, dumplings, couscous and beer. It is also used as animal feed and forage in both temperate and tropical regions because it has a capability to grow well not only in the fertile soil, but also in the poor and dry soil. Most of the current breeding procedures used in pearl millet are aimed at maximum exploitation of hybrid vigor for both grain and forage yields in Korea. Pearl millet is ideally suited for exploitation of heterosis using cytoplasmic male sterile lines as seed parent, and fertile inbred lines and open-pollinated cultivars as pollen parent. Pearl millet hybrids developed in Korea produced 3 to 7 tons of grain and 100 to 150 tons of green fodder per hectare.

• Journal of Korean Society of Forest Science
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• v.105 no.1
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• pp.63-72
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• 2016

A study was conducted to evaluate the effect of collected region(Bongpyeong in Gangwon, 690 m, Mt. Halla in Jeju, 1,085 m), pre-treatment (stratification, soaked in distilled water or $GA_3$) and shading (control, 50%, 80%) on seed emergence and seedlings growth of Ligularia fischeri. The result showed that seed emergence (%) of L. fischeri were 6.9~75.5% (Gangwon : 6.9~32.8%, Halla : 22.2~75.5%). Maximum seed emergence (%) of 32.8 (Gangwon) and 75.5 (Halla) were achieved for seeds were stratified, soaked in distilled water, and under non-shading for L. fischeri (Gangwon), whereas seeds were stratified, soaked in $GA_3$ 100 ppm, and under 80% shading for L. fischeri (Halla). In this study, the best seedling vigor index (SVI) of L. fischeri (Gangwon) and L. fischeri (Halla) were obtained when seeds were stratified, soaked in distilled water, and under 50% shading. The survival rate of potted seedlings for L. fischeri (Halla) were 100% of all shading conditions, but there were decreased by 77.8%, 61.1%, 55.6%, as an increasing shading rate (con, 50%, 80%) for L. fischeri (Gangwon). Therefore, good seedlings were obtained when seed (Mt. Halla) of L. fischeri were stratified, soaked in distilled water, and under 50% shading, and then seedlings were transferred in pot.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.161-161
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• 2017

Seed endophytes are very remarkable groups of bacteria for their unique abilities of being vertically transmitted and conserved. As plants attain hybrid vigor and heterosis in the process of crossbreeding, this might also lead to the changes in the community structure and diversity of plant endophytes in the hybrid plants ultimately affecting the endophytes of the seeds. It would be interesting to characterize how seed endophyte composition change over time. The objective of this study is to gain insights into the influence of natural crossbreeding and parental lineage in the seed bacterial endophytic communities of two pure inbred lines exploring contributions of the two most important sources of plant endophytes - colonization from external sources and vertical transmission via seeds. Total genomic DNA was isolated from rice seeds and bacterial DNA was selectively amplified by PCR. The diversity of endophytic bacteria was studied through Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis. Diversity between the original parents and the pure inbred line may show significant differences in terms of richness, evenness and diversity indices. Heat maps reveal astonishing contributions of both or either parents (IR29 ${\times}$ Pokkali and AT401 ${\times}$ IR31868) in the shaping of the bacterial seed endophytes of the hybrid, FL478 and IC32, respectively. Most of the T-RFs of the subsequent pure inbred line could be traced to any or both of the parents. Comparison of common and genotype-specific T-RFs of parents and their offspring reveals that majority of the T-RFs are shared suggesting higher transmission of bacterial communities common to both parents. The parents influence the bacterial community of their offspring. Unique T-RFs of the offspring also suggest external sources of colonization particularly as the seeds are cultivated in different ecogeographical locations. This study showed that host parental lines contributed greatly in the shaping of bacterial seed endophytes of their offspring. It also revealed transmission and potential conservation of core seed bacterial endophytes that generally become the dominant microbiota in the succeeding generations of plant hosts.