• Journal of Life Science
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• v.28 no.4
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• pp.398-407
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• 2018

The Purple pericarp (Prp) trait is a trait often bred for in black rice. Generally, the Prp trait is displayed in the color variations of seeds following the 9:3:4 purple, brown, and white ratio, respectively. The Prp trait is a recessive epistasis of two gene interactions; however, it is caused by the two complementation genes Pb and Pp. Here we present a study of the genetic characteristics of the Prp trait using an $F_1$ hybrid with a Pbpb Pppp genotype. This hybrid generated four seed colors with the following numbers: 3 dark purple, 6 medium purple, 3 brown, and 4 white (or 9 purple, 3 brown, and 4 white). However, further biochemical analysis of the all progenies divided them into two groups. One group had the Pb_ Pp_ allelic constitutions and contained cyanidin 3-O-glucoside (C3G) in both the dark purple or medium purple seeds. The other group, however, was absent of C3G in both the brown and white seeds, resulting in a ratio of 9:7, respectively. This segregation revealed the extended Mendelian 9:7 ratios of the complementary gene interactions with a good fitness in ${\chi}^2$ analysis. Further analysis revealed that brown seeds with the Pb_ pppp genotype corresponded with a null C3G, indicating that the Brown pericarp trait in rice is caused by a dominant allele of the Pb gene. Therefore, we conclude that the production of C3G is a main phenotype of the black and purple colored rice in the Prp trait, and it is governed by the complementary gene interactions between Pb and Pp genes.

• Journal of Aquaculture
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• v.4 no.2
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• pp.73-84
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• 1991

Underground sea water is widely utilized for land based aquaculture in Cheju province, Korea nowadays. Salinity of this water($20{\~}30$ ppt) is slightly lower than natural sea water but because of its good water quality and temperature which is maintained at $16{\~}18^{\circ}C$ all year-round, many fish farmers are using this water for finfish culture. To evaluate the possibility of utilizing underground sea water for the seed production of the flounder, Paralichthys olivaeceus, hatching rate of fertilized eggs, survival rate of hatched larvae, and growth rate of the juveniles were tested with various salinity levels ranged from 14.1 ppt to 40.2 ppt. The salinity ranges which showed over $70{\%}$ survival rate for fertilized eggs to hatchout were 33.7 ppt${\~}$40.2 ppt, for hatched larvae to 2-day-old were 20.6 ppt${\~}$40.2 ppt, from 10-day-old to 12-day-old were 27.2 ppt${\~}$40.2 ppt, and from 45-day-old to 47-day-old were 20.6 ppt${\~}$40.2 ppt. Two hundred day old fingerlings showed no mortality when exposured to 7.6 ppt${\~}$40.2 ppt for 2 days. All fish died within 3 days when 45-day-old juveniles were transferred directly from normal sea water to 14.1 ppt sea water. However, they were all survived when transferred first to 20.6 ppt for 3 days then to 14.1 ppt sea water. The highest growth rates of fingerlings reared at 4 different salinities (33.7, 27.0, 31.0, or 15 ppt) were obtained at the 27 ppt group followed by 33.7, 21.0, 15 ppt respectively. The relationship between the days of rearing (X) and the total length of the fingerlings were as follows. $33.7\%$ group : Y=8.8109 + 0.1104X(r=0.999) $27.0{\%}$ group : Y=8.6797 + 0.1208X(r=0.997) $21.0{\%}$ group : Y=8.4081 + 0.1052X(r=0.990) $15.0{\%}$ group : Y=8.4309+ 0.0995X(r=0.990)

• Journal of Aquaculture
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• v.9 no.1
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• pp.43-55
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• 1996

The experiment was performed to evaluate the possibility of utilizing underground sea water for the seed production of tiger puffer, Takifugu rubripes. For this purpose, the effects of 6 different salinities (3.5, 7.0, 14.0, 20.0, 27.0, $33.0\%_{\circ}$) were determined based on the hatching rate of fertilized eggs, survival rate and the amount of food consumed by hatched larvae, where as the effects of 3 different salinities (20.0, 27.0, $33.0\%_{\circ}$) were also examined with rearing tiger puffer juvenile ($4.29{\pm}0.50$ cm in total length) for 50 days in the closed recirculating water system. As a results, either the hatching or the survival rate of more than $70.0\%$ were obtained from the fertilized eggs reared at the salinity of 27.0 to $33.0\%_{\circ}$, the early hatched larvae at 27.0 to $33.0\%_{\circ}$, and the 10-day-old larvae at 20 to $33.0\%_{\circ}$. At three different salinities, the survival rate of 20-day- and 30-day-old larvae turned out to be $89.0\%$ and $92.5\%$, respectively. The salinity for maximum food intake thus appeared to be from 27.0 to $33.0\%_{\circ}$. In this condition, 20-day-old hatched larvae consumed $323\~342$ Artemia nauplii and 30-day-old hatched larvae ate $1,559\~1,5791$ A. nauplii. The highest growth rate of fingerlings were observed at the salinity $27\%$ and followed by 33.0 and $20.0\%_{\circ}$, respectively. The relationship between the days of rearing (X) and the total length (Y) of the fingerlings were as follows : $33.0\%_{\circ}$ group : Y=0.107X-2.532 (r=0.982) $27.0\%_{\circ}$ group : Y=0.116X-3.195 (r=0.975) $20.0\%_{\circ}$ group : Y=0.116X-2.693 (r=0.987) The slopes of regression line estimated from $27.0\%_{\circ}$ and $33.0\%_{\circ}$ groups were significantly different from that of $20.0\%_{\circ}$ group.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.21 no.4
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• pp.206-216
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• 1988

In order to set up a predictive model for effective spat collection of pen shell, Atrina (Servatrina) pectinata, the survival rate and time required at each developmental stage of drifting larvae were surveyed during the period from June 8 to October 16 in 1986 at the Yongku inlet Chilchon Is., Chinhae Bay, the southern part of Korea. And also the experiments of spat collection were carried out In Yongku inlet during the period from July 6 to November 23 in 1987 and In Yoja Bay during the period from July 9 in 1987 to February 15 in 1588. The advent of D-shape larvae ca. $135\times144um $ long had three peaks in that area: August 1, 12 and 25. Umbo shape larvae ca. $300\times317um$ and full grown larvae ca. $455\times450um$ long also sowed three peaks: August 9, August 22 and September 4 for the former, and August 23, September 3 and September 16 for the latter. Nine to ten days was required for D-shape larvae to develop to umbo shape larvae. The instantaneous survival rate was 0.94 with a total survival rate if $54\%$ at this intermorphological stage. The required time of umbo to full grown larvae varied from twelve to fourteen days with a instantaneous survival rate of 0.88 and total survival rate of $19\%$. Twenty-two to twenty-three days was required for each group of the D-shape larvae to reach a fullgrown stage, and their total survival rate was $10\%$ during this developmental period. The number of the spat attached to the spat collector is 0.16 inds. per $m^2$ vertical spat collector in Yongku inlet and 0.48 inds. per $m^2$ horizontal spat collector in Yoja Bay. The average shell length of spat attached was $0.51({\pm}0.15)\;mm$ on September $27,\;38.52({\pm}6.98)\;mm$ on November 21 in 1987 and $49.00({\pm}10.77)mm$ on February 15 in 1988.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.61 no.4
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• pp.277-282
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• 2016

The consumption of waxy corn is steadily increasing in Korea. Waxy corn is harvested before it reaches full maturity and consumed immediately or follwing cold storage. Glutinous and sweet kernels are preferred due to their high palatability. The kernel properties can change rapidly following harvest, and, therefore, optimal conditions to maintain the kernel quality of corn should be identified. In addition, the timing of harvest of waxy corn ear should be also determined for optimal marketable corn production. From 10 days after silking (DAS) to25 DAS. fresh ear weight and single kernel weight rapidly increased from 78.3 g and 1.13 g, respectively, to 224.9 and 3.61 g, respectively. However, by 30 DAS both fresh and single kernel weight decreased by 10.6% and 6.1%, respectively. Kernel hardness significantly increased up to 25 DAS, and a further slight increase in kernel hardness was observed at 30 DAS. Total sugar content in kernel decreased from 12.5% at 10 DAS to 3.5% at 35 DAS, which was the result of the conversion of sugars to starch during ear development. Crude protein content in kernel did not vary significantly in comparison to kernel hardness. During storage of ear, kernel hardness increased from $726g\;cm^{-2}$ at harvest to $1894g\;cm^{-2}$ following 28 days of storage at a low temperature ($0^{\circ}C$). Kernel hardness increased 2.5 fold from 15 DAS to 30 DAS. Soluble protein level in kernel increased until 10 DAS, following which a slight decrease was observed. The soluble protein content decreased from 1.85% at 5 DAS to 1.45% at 35 DAS. Total sugar content in kernel decreased regardless of storage temperature; however, the rate of reduction was lower at $0^{\circ}C$ than that observed following storage at $4^{\circ}C$ and $10^{\circ}C$. The rate of reduction in kernel moisture content was also lower at $0^{\circ}C$ than that observed at $4^{\circ}C$ and $10^{\circ}C$.

• Korean Journal of Weed Science
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• v.8 no.3
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• pp.309-316
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• 1988

Several experiments were conducted to investigate the achene viability and growth characteristics of Eclipta prostrata (L.) L. No dormancy and no after-ripening requirement were found for E. prostrata achenes. When achenes were stored at room temperature, germination did not decrease with up to 5 months storage. Large differences in loss of viability of E. prostrata achenes occurred when different dehydration methods were used. Immediate dehydration resulted in high viability, but slow dehydration resulted in severe loss of viability. Achene viability at shallow burial depths (5 and 10 cm deep) was lower under upland soil conditions than under lowland soil conditions. Seedling growth was greatly reduced when flooding to a depth of 10 cm occurred at or before the 4-leaf stage. Flooding after the 4-leaf stage stimulated stem elongation. Branching started from the second week and usually terminated at the tenth week. Leaf size was determined by the branch which are related to the assimilate supply. Flowering of E. prostrata started during the fifth week after emergence, and mature achenes were produced from the sixth week. Ten to 14 days were needed for the achenes to mature. About 14,000 achenes were produced on each plant. Achene production per week increased from the sixth week to the tenth week and thereafter it declined. The average number of achenes per inflorescence decreased with delay in flowering.

• Horticultural Science & Technology
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• v.18 no.3
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• pp.334-341
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• 2000

To develop a nutrient solution for a closed hydroponic system in potato (Solanum tuberosum L.) 'Atlantic' and 'Superior' potatoes were grown with the nutrient solutions whose strengths were 0.25, 0.5, 1.0, and 1.5 of the concentration of the nutrient solution developed by the National Horticultural Experiment Station in Japan. The best results in potato growth and yield were obtained with 0.5 and 1.0 strength nutrient solutions, and nutrient compositions for potato were determined based on the 1.0 strength nutrient solution; $14.4me{\cdot}L^{-1}\;N,\;4.2me{\cdot}L^{-1}\;P,\;7.5me{\cdot}L^{-1}\;K,\;5.5me{\cdot}L^{-1}\;Ca$, and $3.5me{\cdot}L^{-1}\;Mg$ for stolon growth stage and $14.8me{\cdot}L^{-1}\;N,\;4.0me{\cdot}L^{-1}\;P,\;8.5me{\cdot}L^{-1}\;K,\;6.5me{\cdot}L^{-1}\;Ca$, and $3.0me{\cdot}L^{-1}\;Mg$ for tuber growth stage. To examine the suitability of the nutrient solutions developed for potato, the strengths of 1.0 (PS 1.0S), 0.75 (PS 0.75S), or 0.5 (PS 0.5S) were compared with half-strength of Japanese Horticultural Experiment Station' solution (JH 0.5S). Changes in pH, EC, and mineral concentrations in nutrient solutions depended more on solution strength and growth stage than on the type of nutrient solution. However, most elements in solution remained constant with plant age in PS 0.75S solution during stolon growth stage, and in PS 0.5S solution during tuber growth stage. The greatest growth and tuber yield was obtained in the standard strength solution (PS 1.0S), and potato solution developed in this experiment was recommended for hydroponic culture of potato in a closed system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.3
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• pp.233-240
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• 2017

This experiment was conducted to establish a cultivation system for sorghum in reclaimed soils. Pot experiments were used to test the effects on seedling establishment of sowing depth, soil water content, and soil salinity using seeds of Nampungchal-susu and Hwanggeumchal-susu in reclaimed soil. Field experiments were also conducted to examine differences in growth characteristics and yield production, by sowing time, and planting distance. The result of the pot experiment, examining seedling establishment at various sowing depths revealed that, it was the highest 76.7% when the seeds were sown at a depth of 3 cm. Seedling establishment did not differ with soil water content between 10~30 kpa and at 51~70 kpa. No effects of seed moisture absorption before sowing were observed. Seedling establishment showed no differences with soil salinity below $3.2\;dS\;m^{-1}$, but decreased with Salinity above $4.8\;dS\;m^{-1}$. In field experiments to assess the effects on seedling establishment ratio of sowing time, Nampungchal-susu was revealed to have a high seedling establishment ratio following sowing on June 15. Hwanggeumchal-susu did not exhibits effects of sowing time, on seedling establishment ratio but exhibited higher seedling establishment when in low soil salinity conditions than when sown in high soil salinity conditions. With respect to yield, the yield of the seeds sown on June 15 was higher by 13% for Nampungchal-susu and by 29% for the Hwanggeumchal-susu than that those sown on June 25. With respect to soil salinity, the yield at a soil salinity of $3.2dS\;m^{-1}$ was lower by 23% than that at $1.6dS\;m^{-1}$ or lower for Nampungchal-susu, and was lower by 30% Hwanggeumchal-susu. With respect to planting density, both breeds showed the highest yield at $60{\times}10cm$. These results suggest that a sowing time of June 15 and a seeding distance of $60{\times}10cm$ are appropriate for sorghum in reclaimed land.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.284-288
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• 2005

This study was conducted to clarify the effects of supplying methods of selenium on the growth and Se uptake of hydroponically grown tomato plants. Tomato seeds (Lycopersicum esculentum Mill. cv. Momotaro T-93, Daki Seed Co.) were sown in plug tray with fifty holes, and raised for sixty days. Tomato seedlings transplanted to coco fiber slabs were supplied with the nutrient solutions adjusted to EC $2.3dS{\cdot}m^{-1}$ and pH $5.8\~6.2$ recommended by the Japanese Horticultural Experiment Station. Selenium forms used were inorganic $SeO_2$ (here in after referred to Se) and organic selenium chlenium with sugar fatty acid ester (here in after referred to chelated-Se). 10 ppm selenium solutions were treated to tomato plants with foliar applications, drenching, and foliar application plus drenching. Growth characteristics in terms of plant height, number of leaves, leaf area and chlorophyll content were significantly increased in the plot of foliar application ot Se, and in the plot of foliar application plus drenching of chelated-Se than other plots, respectively. Transported contents of selenium into the tomato fruits were highest as 0.302 ppm in the plot of foliar application plus drenching of chelated-Se. Also, it had tended to be higher in the plot of foliar application plus drenching than in the plots of foliar application or drenching in both of Se and chelated-Se. Foliar application and drenching of organic chelated-Se were effective to produce the functional tomato fruits.

• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.441-449
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• 2013

This study was conducted to select some useful plants as functional material candidates. A total of 38 plants were preliminarily screened for the anti-inflammatory and antioxidant activities. The preliminarily selected 8 plants were further investigated to verify the in vitro inhibitory effect on inflammation and oxidative stress. Boehmeria platanifolia (root), Carpinus coreana (branch), and Eupatorium japonicum (leaf) inhibited the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-treated RAW 264.7 cells. Eupatorium japonicum (leaf) suppressed the expression of cyclooxygenase-2 (COX-2), whereas Boehmeria platanifolia (root) and Prunus yedoensis (branch) inhibited the transcription of nuclear factor-kappa B (NF-${\kappa}B$). Treatment with the extracts ($2.5{\sim}20{\mu}g/ml$) of Abutilon theophrasti (leaf, flower/seed) and Hemistepta lyrata (stem) did not show toxicity on RAW 264.7 cell proliferation, but treatment with $2.5{\mu}g/ml$ of Boehmeria platanifolia (root) exhibited cell toxicity. Carpinus coreana (branch) and Prunus yedoensis (branch) showed potent scavenging activities on peroxynitrite. Akebia quinata (flower), Carpinus coreana (branch), and Prunus yedoensis (branch) effectively inhibited reactive oxygen species (ROS). Abutilon theophrasti (leaf), Boehmeria platanifolia (root), Carpinus coreana (branch), and Eupatorium japonicum (leaf) exhibited strong inhibitory capacity with regard to nitric oxide (NO) production. The results suggested that Abutilon theophrasti (leaf) has in vitro anti-inflammatory and antioxidant activities, and that is a useful functional material candidate.