• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.41 no.3
• /
• pp.340-347
• /
• 1996

Soybean [Glycjne max Merr. L.] variety 'Danyeobkong' was inoculated with soybean mosaic virus SMV-G5H strain in field and green house at Crop Experiment Station in 1995. The effects of this virus strain on plant characteristics and seed chemical composition of the soybean variety Danyeobkong were evaluated. Yield, the number of pod and 100 seed weight were significantly reduced by SMV-G5H strain infection. Inoculation of SMV-G5H strain of soybean mosaic virus caused the increment of seed protein content and the decrement of seed oil content in the soybean variety Danyeobkong. Changing pattern of fatty acid composition by SMV-G5H strain infection was varied according to the experimental place. In field trial, the percentage of linoleic and linolenic acids decreased and stearic and oleic acids increased in the seed oil from virus infected plants. In green house trial, however, linolenic and oleic acids were increased, whereas palmitic and linoleic acids were reduced.

• BMB Reports
• /
• v.48 no.12
• /
• pp.643-644
• /
• 2015

In eukaryotes, small RNAs play important roles in both gene regulation and resistance to viral infection. Argonaute proteins have been identified as a key component of the effector complexes of various RNA-silencing pathways, but the mechanistic roles of Argonaute proteins in these pathways are not clearly understood. To address this question, we performed single-molecule fluorescence experiments using an RNA-induced silencing complex (core-RISC) composed of a small RNA and human Argonaute 2. We found that target binding of core-RISC starts at the seed region of the guide RNA. After target binding, four distinct reactions followed: target cleavage, transient binding, stable binding, and Argonaute unloading. Target cleavage required extensive sequence complementarity and accelerated core-RISC dissociation for recycling. In contrast, the stable binding of core-RISC to target RNAs required seed-match only, suggesting a potential explanation for the seed-match rule of microRNA (miRNA) target selection.

• Natural Product Sciences
• /
• v.9 no.2
• /
• pp.60-63
• /
• 2003

Effect of grape seed extract (GSE) against Candida albicans was examined under in-vitro and in-vivo conditions. The GSE was extracted in ethanol. In-vitro results from an agar diffusion susceptibility assay showed the GSE inhibited C. albicans growth. This anticandidal effect was at dose-dependency. In experiments with animals, mice that received the GSE (0.5 mg per mice), intravenously (i.v.), before i.v.-infection wish viable C. albicans yeast cells survived longer than diluent (buffer)-received control mice. In contrast, when GSE was given to mice after the mice were infected with the yeast cells, these mice showed a similar survival rate as compared to control mice that received no treatment with the GSE. Taken together, these data indicate that GSE has prophylactic effect but not therapeutic effect against disseminated candidiasis.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.35 no.1
• /
• pp.1-11
• /
• 2015

In this study, an online seed potato sorting system using a visible and near infrared (40 1100 nm) transmittance spectral technique and statistical model was evaluated for the nondestructive determination of infected and sound seed potatoes. Seed potatoes that had been artificially infected with Pectobacterium atrosepticum, which is known to cause a soil borne disease infection, were prepared for the experiments. After acquiring transmittance spectra from sound and infected seed potatoes, a determination algorithm for detecting infected seed potatoes was developed using the partial least square discriminant analysis method. The coefficient of determination($R^2_p$) of the prediction model was 0.943, and the classification accuracy was above 99% (n = 80) for discriminating diseased seed potatoes from sound ones. This online sorting system has good potential for developing a technique to detect agricultural products that are infected and contaminated by pathogens.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2002.11a
• /
• pp.71-71
• /
• 2002

• The Plant Pathology Journal
• /
• v.36 no.1
• /
• pp.29-42
• /
• 2020

Acidovorax citrulli causes bacterial fruit blotch in Cucurbitaceae, including watermelon. Although A. citrulli is a seed-borne pathogen, it can cause diverse symptoms in other plant organs like leaves, stems and fruits. To determine the infection routes of A. citrulli, we examined the virulence of six isolates (Ac0, Ac1, Ac2, Ac4, Ac8, and Ac11) on watermelon using several inoculation methods. Among six isolates, DNA polymorphism reveals that three isolates Ac0, Ac1, and Ac4 belong to Clonal Complex (CC) group II and the others do CC group I. Ac0, Ac4, and Ac8 isolates efficiently infected seeds during germination in soil, and Ac0 and Ac4 also infected the roots of watermelon seedlings wounded prior to inoculation. Infection through leaves was successful only by three isolates belonging to CC group II, and two of these also infected the mature watermelon fruits. Ac2 did not cause the disease in all assays. Interestingly, three putative type III effectors (Aave_2166, Aave_2708, and Aave_3062) with intact forms were only found in CC group II. Overall, our results indicate that A. citrulli can infect watermelons through diverse routes, and the CC grouping of A. citrulli was only correlated with virulence in leaf infection assays.

• The Plant Pathology Journal
• /
• v.21 no.3
• /
• pp.297-300
• /
• 2005

Racodium therryanum from Korea is described here for the first time. This fungus is characterized by that it is isolated from conifer seeds and seedlings, including Abies, colonies are dark green to dark gray and intricate, and it forms chlamydospores in cultures. This pathogenic fungus was isolated from seeds of Abies koreana growing on Mt. Halla, Jeju island. The fungus stopped growth more than at $30^{\circ}C$ but grew even at $O^{\circ}C$. This fungus infected seeds of A. koreana under the snow during winter season. There is a high probability that this fungus affects the natural regeneration of A. koreana on Mt. Halla.

• Korean Journal Plant Pathology
• /
• v.14 no.4
• /
• pp.287-293
• /
• 1998

Bipolaris coicis, B. cynodontis, B. maydis and Curvularia lunata were leaf blight fungi detected from 45 seed samples of Job's tears and B. coicis was the predominant species in seed samples followed by C. lunata, B. cynodontis and B. maydis. When the seed components were plated on test tube agar, B. coicis and C. lunata were highly detected from invelucre, glume, endosperm and stamen, but not detected from plumule. Seed infection with B. coicis casued seed rot, coleoptile blight and seedling blight of Job's tears. Conidial characteristics of leaf blight fungi were as follows; B. cynodontis was fusiform, brown, slightly curved, 0~5 distoseptate, and 16.8-48$\times$7.2-16.8${\mu}{\textrm}{m}$ in size; B. cynodontis was fusiform, brown, slightly curved, 0~8 distoseptate, and 16.8-72$\times$9.6-19.2 ${\mu}{\textrm}{m}$; B. maydis was fusiform, brown, distintly curved, 0~10 distoseptate, and 28.8-110.4$\times$12-21.6 ${\mu}{\textrm}{m}$. C. Lunata was fusiform, brown, typically curved, 0~3 distoseptate, and 7.2-24$\times$4.8-12 ${\mu}{\textrm}{m}$. B. coicis was highly pathogenic to Job's tears and corn, weakly pathogenic to rice, but not pathogenic to wheat and barley. c. lunata was highly pathogenic to Job's tears (No. 2), corn, wheat and barley, weakly pathogenic to Job's tears (No. 1), but not pathogenic to rice. All treatments were effective to inhibition of leaf blight fungi when carboram, benoram, fludioxonil, prochloraz, thioram, and tap water treated to infected seeds.

• Korean journal of applied entomology
• /
• v.21 no.1 s.50
• /
• pp.23-26
• /
• 1982

The present study was carried out to investigate the change of peroxidase activity of soybean seed infected with Cerrospora kikurhii. The protein content, polyphenol oxidase activity and peroxidase isozyme pattern in health and infected soybean seed were also compared. 1. The peroxidase activity was substantially higher in the infected soybean seeds than that in the healthy seeds either cracked or not. No significant differences in protein content were recognized among the seeds tested. 2. No significant differences in peroxidase activities and protein contents were notified between healthy and infected seeds from the measurements on each parts of dissected seeds, cotyledon and seedcoat, however the peroxidase activity in the seed coat of the stained seed was 2.5 times to healthy seed. 3. The activities of polyphenel oxidase were undectable in both healthy and diseased seeds. 4. The electrophoretic Patterns of the Peroridase isozyme were the same between healthy and in footed seed. 5. Therefore, the increase of peroxidase activity in infected soybean seedcoat was mainly due to the biochemical reaction against the pathogen.

• Korean Journal of Agricultural Science
• /
• v.42 no.4
• /
• pp.305-311
• /
• 2015

In this research, we surveyed the yield change of garlic cultivation with seed bulbs according to annual field culture after induced meristem culture. From 2007 to 2013, yearly produced seed bulbs from $2^{nd}$ to $8^{th}$ generation were cultivated in the Muan field. Over the generations, the general growth characteristics such as the plant height, leaf number, leaf length and stem diameter were all decreased. When we compared the conventional seed bulbs and TCR ones, the plant height and stem diameter showed bigger difference. And the TCR seed bulbs showed a week late harvest time against the conventional ones. The yield of $2^{nd}$ generation was 72.3 g and 57.1 g for the $8^{th}$ generation. However every generation showed higher yield than conventional seed garlic's ones as 43.9 g. The yield index showed the same trend as $2^{nd}-8^{th}$ to 63% to 21%. After the tissue culture of garlics, the viral infections (OYDV and GSV) of TCR was far less than that of conventional ones. In case of LYSV, the infection ratio was 6.7% at $2^{nd}$ generation and 26% at $8^{th}$. However, the ratio was far less than that (80%) of the conventional ones.