• Title/Summary/Keyword: seed filter

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Preparation of PMSQ/TiO2 Composite Fine Powder by Sol-Gel Process (Sol-Gel Process를 이용한 PMSQ/TiO2 복합 미립자의 합성)

  • Lee, Dong Hyun;Koo, Sangman
    • Applied Chemistry for Engineering
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    • v.9 no.5
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    • pp.634-638
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    • 1998
  • Monodisperse, spherical $PMSQ/TiO_2$ composite fine powders were prepared by modified sol-gel process where 300 nm $TiO_2$ fine powders were used as seed particles for hetero-condensation with hydrolyzed MTMS (Methyltrimethoxysilane). The reaction was carried out under $N_2$ atmosphere at ambient temperature using $NH_3$ as a catalyst. Methanol was used as a solvent. Powder was obtained by the filtration of the solution with a glass filter and washing with acetone. The stirring rate, reaction temperature, $[H_2O]/[MTMS]$ and $[MTMS]/[TiO_2]$ ratio were varied to investigate shapes and sizes of particles. Monodisperse particles of $1-2{\mu}m$ diameter were obtained with [MTMS]=0.2 M, $[NH_3]=0.6M$, $[H_2O]/[MTMS]=100$, $[MTMS]/[TiO_2]=10-50$ at ambient temperature with mild stirring condition. These composite particles had a contact angle of almost 180 degree contact angle with water, which proves their excellent hydrophobicity. The study of UV absorption spectra showed that they have UV protecting effect.

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A two-stage cascaded foreground seeds generation for parametric min-cuts

  • Li, Shao-Mei;Zhu, Jun-Guang;Gao, Chao;Li, Chun-Wei
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.10 no.11
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    • pp.5563-5582
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    • 2016
  • Parametric min-cuts is an object proposal algorithm, which can be used for accurate image segmentation. In parametric min-cuts, foreground seeds generation plays an important role since the number and quality of foreground seeds have great effect on its efficiency and accuracy. To improve the performance of parametric min-cuts, this paper proposes a new framework for foreground seeds generation. First, to increase the odds of finding objects, saliency detection at multiple scales is used to generate a large set of diverse candidate seeds. Second, to further select good-quality seeds, a two-stage cascaded ranking classifier is used to filter and rank the candidates based on their appearance features. Experimental results show that parametric min-cuts using our seeding strategy can obtain a relative small pool of proposals with high accuracy.

Effect of Seed Priming and Pellet Coating Materials on Seedling Emergence of Aster koraiensis (프라이밍과 펠렛코팅 소재가 벌개미취 종자의 유묘 출현율에 미치는 영향)

  • Kang, Won Sik;Kim, Min Geun;Kim, Soo Young;Han, Sim Hee;Kim, Du Hyun
    • Journal of Korean Society of Forest Science
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    • v.109 no.1
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    • pp.41-49
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    • 2020
  • In this study, the effect of seed pre-treatments and pellet coating materials to enhance the efficiency of large-scale propagation of Aster koraiensis seeds were investigated. Seeds were immersed in water for one day, and only those that sank were used for pre-treatment to use filled seeds. Pre-treatments were divided into hormone treatments, with gibberellic acid (GA3; 200 and 500 ppm) and 24-epibrassinolide (10-6, 10-7, and 10-8M), and priming with potassium nitrate (100 mM of KNO3). To produce pellet-coated seeds, pellet materials (DTCS or DTK) were applied to control (unprimed) and primed seeds with binders (PVA or CMC). The maximum germination percent (GP) of seeds before pellet coating was 65% (with the priming treatment), and there was no difference in the GP of seeds among hormone treatments. For seeds sown in a growth chamber on filter paper, GP was 41% for control (unprimed/uncoated) seeds, 65% for uncoated primed seeds, 71% for DTCS/PVA-pellet-coated seeds, and 42% for DTK/CMC-pellet-coated seeds. Seeds that were primed first and then pellet-coated showed greatly improved GP, mean germination time (MGT), and germination rate than seeds that were only pellet-coated. For seeds sown in commercial soil in a greenhouse, control seeds had a GP of 27%, whereas primed seeds had the highest GP (58%), and their MGT and GT were 9.4 days and 7.0%·day, respectively. In addition, DTK/PVA-pellet-coated seeds (40%) also had a GP higher than the control (27%), and their MGT was 15-27 days. For seeds sown in sandy-loam soil in a greenhouse, unprimed-pellet-coated seeds and primed-pellet-coated seeds both had GPs ranged of 39%, which were lower than that of control seeds. In general, the seeds that were pellet-coated with DTK had a higher GP than those pellet-coated with DTCS. Furthermore, the MGT of unprimed-pellet-coated seeds was 15.0-19.8 days, which was longer than the MGT of primed-pellet-coated seeds. These results suggest that priming enhances seedling emergence of Aster koraiensis seeds. Moreover, when priming is combined with pellet coating, DTK is a more suitable pellet material than DTCS, and PVA and CMC are equally suitable adhesives.

Estimation of Cardinal Temperatures for Germination of Seeds from the Common Ice Plant Using Bilinear, Parabolic, and Beta Distribution Models

  • Cha, Mi-Kyung;Park, Kyoung Sub;Cho, Young-Yeol
    • Horticultural Science & Technology
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    • v.34 no.2
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    • pp.236-241
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    • 2016
  • The common ice plant (Mesembryanthemum crystallinum L.) has some medicinal uses and recommended plant in closed-type plant factory. The objective of this study was to estimate the cardinal temperatures for seed germination of the common ice plant using bilinear, parabolic, and beta distribution models. Seeds of the common ice plant were germinated in the dark in a growth chamber at four constant temperatures: 16, 20, 24, and $28^{\circ}C$. For this, four replicates of 100 seeds were placed on two layers of filter paper in a 9-cm petri dish and radicle emergence of 0.1 mm was scored as germination. The times to 50% germination were 4.3, 2.5, 2.0, and 1.8 days at 16, 20, 24, and $28^{\circ}C$, respectively, indicating that the germination of this warm-weather crop increased with temperature. Next, the time course of germination was modeled using a logistic function. For the selection of an accurate model, seeds were germinated in the dark at constant temperatures of 6, 12, 32, and $36^{\circ}C$. Germination started earlier and increased rapidly at temperatures above $20^{\circ}C$. The minimum, optimal, and maximum temperatures were estimated by regression of the inverse of time to 50% germination rate, as a function of the temperature gradient. The different functions estimated differing minimum, optimal and maximum temperatures, with 5.7, 27.7, and $36.5^{\circ}C$, respectively for the bilinear function, 13.4, 25.0, and $36.6^{\circ}C$, respectively, for the parabolic function and 7.8, 25.9, and $36.0^{\circ}C$, respectively, for the beta distribution function. The models estimated that the inverse of time to 50% germination rate was 0 at 6 and $36^{\circ}C$. The observed final germination rates at 12 and $32^{\circ}C$ were 62 and 97%, respectively. Our data show that a beta distribution function provides a useful model for estimating the cardinal temperatures for germination of seed from the common ice plant.

Key factors causing the Euryale ferox endangered hydrophyte in Korea and management strategies for conservation (멸종위기 부엽식물 가시연꽃 개체군의 분포, 멸종 원인 key factor에 대한 실험과 보전을 위한 관리전략)

  • You, Young-Han;Kim, Hae-Ran
    • Journal of Wetlands Research
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    • v.12 no.3
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    • pp.49-56
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    • 2010
  • Euryale ferox is only annual hydrophyte among endangered plants in Korea and its seed is high valuable medicinal material. This studies were carried to find out key factors that made E. ferox endanger in Korea and to apply the results for effective management of the natural wetlands inhabited by E. ferox. We investigated the distributed sites reported by previous studies and classified the distribution-affected factors into three categories, eutrophication(hypothesis 1), soil accumulation(hypothesis 2) and increasing of water level(hypothesis 3) in the habitat. These three hypotheses were tested in the field and explained the results with the morphological characteristics of seed germination stages of E. ferox. The geographical distribution ranges of E. ferox diminished at a rate of $1^{\circ}$ latitude with about 10 natural wetlands for last 30 years. Water eutrophication in the wetland had a positive effect on the establishment of E. ferox. But higher in soil accumulation and water depth, then lower in the establishment rate of E. ferox. These results indicate that water depth in the wetland must be below 50cm during the germination season, especially in May and emergent hydrophytes filter zone is need to protect soil accumulation from soil erosion within its watershed for the effective management of wetland with E. ferox.

Prevention of UV-induced Skin Damage by Activation of Tumor Suppressor Genes p53 and $p14^{ARF}$

  • Petersen, R.;John, S.;Lueder, M.;Borchert, S.
    • Proceedings of the SCSK Conference
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    • 2003.09a
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    • pp.338-351
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    • 2003
  • UV radiation is the most dangerous stress factor among permanent environmental impacts on human skin. Consequences of UV exposure are aberrant tissue architecture, alterations in skin cells including functional changes. Nowadays new kinds of outdoor leisure-time activities and changing environmental conditions make the question of sun protection more important than ever. It is necessary to recognize that self-confident consumers do not consider to change their way of life, they demand modern solutions on the basis of new scientific developments. In the past one fundamental principle of cosmetics was the use of physical and organic filter systems against damaging UV-rays. Today new research results demonstrate that natural protecting cell mechanisms can be activated. Suitable biological actives strongly support the protection function not from the surface but from the inside of the cell. A soy seed preparation (SSP) was proven to stimulate natural skin protective functions. The major functions are an increased energy level and the prevention of DNA damage. These functions can I be defined as biological UV protection. The tumor suppressor protein p53 plays a key role in the regulation of DNA repair. p53 must be transferred into the phosphorylated form to work as transcription factor for genes which are regulating the cell cycle or organizing DNA repair. A pretreatment with SSP increases the phosphorylation rate of p53 of chronically UV-irradiated human keratinocytes significantly. According to the same test procedure SSP induces a dramatic increase in the expression of the tumor suppressor protein p14$^{ARF}$ that is supporting the p53 activity by blocking the antagonist of p53, the oncoprotein Mdm2. Mdm2, a ubiquitin E3-ligase, downregulates p53 and at the same time it prevents phosphorylation of p53. The positive influence of the tumor suppressor proteins explains the stimulation of DNA repair and prevention of sunburn cell formation by SSP, which was proven in cell culture experiments. In vivo the increased skin tolerance against UV irradiation by SSP could be confirmed too. We have assumed, that an increased repair potential provides full cell functionality.y.

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A Study on the Effects of Taxus Extracts in Cosmetic Indurstry (화장품산업에서 주목추출물의 효능에 관한 연구)

  • 김인영;이계종;정성원;이주동;유희창;조춘구
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.28 no.1
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    • pp.80-98
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    • 2002
  • Taxus cuspidata Sieb selected cultivation as drug, food and decorative plant in Kyong-gi province in Korea. As a manufacturing method, there were extracted from 250g of dried-leaf and 300g of dried-stem with each 200g of BG, PG and water (to 100) mixing for 72 hour at 50$\pm$5$\^{C}$ and then they were filtered by 400-mesh filter. Appearance of extract of leaves was slight brown, pH=5.3$\pm$0.5, gravity was 1.012$\pm$0.05, and a reflective index was 1.375$\pm$0.05. And appearance of extract of stems was slightly dark brown, pH=5.4$\pm$0.5, gravity was 1.016$\pm$0.05, and a reflective index was 1.358$\pm$0.05. It was extracted oil from Taxus seed. Gravity was 0.922$\pm$0.05 and it should be obtained the 27.0$\pm$0.5% of yield. The molecular weight of polysaccharide was about 50,000 to 300,000 dalton and contained 5.0$\pm$1.2% of yield from Taxus fruit. The determinations of total polyphenols in measuring spectropotometer got 0.563% in leaves, and 0.325% in stems, whereas the quantitives of total tannins got 0.054% and 0.037%, respectively. As the effects in Cosmetics by DPPH-method, the antioxidative activities were very strong that the inhibitory ratio showed 75% in leaves and 64% in stems compared with 52% in greentea extract. These are more effective than other plant extracts. The increasing ratio of collagen synthesis rate on the activating fibroblast for extracts of Taxus cuspidata Sieb showed 54.16% (stems) and 33.18% (leaves), To improve the skin elasticity, PPE(porcine pancreatic elastase)-inhibitory activities were strongly effective as 13,7% (stems), 23.5% (leaves) and 66%(seed). Anti-inflammatory acitvity of seed oil was very the above 41% stronger than SG was 24% of anti-Inflammatory as a control sample.

Effect of Growth Medium and Extract Sterilization on a Seedling Bioassay of Alfalfa Autotoxicity (생장배지 및 추출액의 멸균이 알팔파 Autotoxicity 의 유묘 생물검정에 미치는 영향)

  • Chon, Sang-Uk;Nelson, C.Jerry;Han, Sung-Uk;Guh, Ja-Ock
    • Korean Journal of Environmental Agriculture
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    • v.18 no.2
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    • pp.185-190
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    • 1999
  • Autotoxic substance(s) from alfalfa(Medicago sativa L.) plants reduces germination and growth of adjacent new alfalfa after alfalfa. The autotoxic chemical(s) in alfalfa are clearly unknown. Our objective was to improve the sensitivity of an alfalfa seedling bioassay for evaluating autotoxic leaf extracts. We determined critical extract concentrations that inhibit seed germination and seedling growth, compared two different culture media, and evaluated the effects of extract sterilization on the sensitivity of the assay, by using streptomycin and autoclaving method. An agar medium in petri plate gave better responses of germination and seedling growth to the extracts than using filter paper in the plate. On agar medium, the concentration of extract required to reach 50% inhibition of root length was 2.7 g $kg^{-1}$, and of germination and hypocotyl length were 3.8 and 9.9 g $kg^{-1}$, respectively. Leaf extracts with 100 ppm streptomycin stimulated germination significantly compared to Leaf extract alone but reduced root length of control by 43%. Root length was more sensitive to the autotoxin(s) than was germination or hypocotyl length. These results suggest that agar medium mixed with extract and sterilization by autoclaving could be improved the consistency and precision of bioassay, and that root length was the best parameter of autotoxic effect of alfalfa leaf extract.

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The Acceleration of Germination in Welsh Onion Seed Irradiated with the Low Dose ${\gamma}-ray$ Radiation (저선량 감마선 조사가 파종자의 발아에 비치는 영향)

  • Lee, Eun-Kyung;Kim, Jae-Sung;Lee, Young-Keun;Lee, Young-Bok
    • Korean Journal of Environmental Agriculture
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    • v.17 no.4
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    • pp.346-351
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    • 1998
  • To investigate the hormetic effects of the low dose ${\gamma}-ray$ radiation on the germination rate, Welsh onion (Allium fistulosum L. cv. Eunchun and cv. Sukchangwoidae) seeds were irradiated at the dose of $0.5\;{\sim}24.0$ Gy with the ${\gamma}-ray$ radiation (Co-60). The germination rate of 'Eunchun' cultivar increased about 10% in the low dose ${\gamma}-ray$ irradiation group compared with that of the control. In the 'Sukchangwoidae' cultivar, the germination rate of the 4 Gy irradiation group increased 40% more than that of the control. Broadly, it seemed that the hormetic effects of the low-dose ${\gamma}-ray$ radiation were taken more promisingly in the uncultivated soil than in the fertile soil. The germination rate from the paper towel and filter paper based cultivation increased 10% and 16% more, respectively, in the 1 Gy irradiation group than that in the control group. And the electric conductivities of the above groups supposed to be taken hormetic effects of the ${\gamma}-ray$ radiation were lower than those of the control group. From the above results, it is suggested that the low dose ${\gamma}-ray$ radiation ranged from 1 Gy to 10 Gy could have the hormetic effects on the germination rate related characters in Welsh onion seeds.

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Hybrid MBE Growth of Crack-Free GaN Layers on Si (110) Substrates

  • Park, Cheol-Hyeon;O, Jae-Eung;No, Yeong-Gyun;Lee, Sang-Tae;Kim, Mun-Deok
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.183-184
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    • 2013
  • Two main MBE growth techniques have been used: plasma-assisted MBE (PA-MBE), which utilizes a rf plasma to supply active nitrogen, and ammonia MBE, in which nitrogen is supplied by pyrolysis of NH3 on the sample surface during growth. PA-MBE is typically performed under metal-rich growth conditions, which results in the formation of gallium droplets on the sample surface and a narrow range of conditions for optimal growth. In contrast, high-quality GaN films can be grown by ammonia MBE under an excess nitrogen flux, which in principle should result in improved device uniformity due to the elimination of droplets and wider range of stable growth conditions. A drawback of ammonia MBE, on the other hand, is a serious memory effect of NH3 condensed on the cryo-panels and the vicinity of heaters, which ruins the control of critical growth stages, i.e. the native oxide desorption and the surface reconstruction, and the accurate control of V/III ratio, especially in the initial stage of seed layer growth. In this paper, we demonstrate that the reliable and reproducible growth of GaN on Si (110) substrates is successfully achieved by combining two MBE growth technologies using rf plasma and ammonia and setting a proper growth protocol. Samples were grown in a MBE system equipped with both a nitrogen rf plasma source (SVT) and an ammonia source. The ammonia gas purity was >99.9999% and further purified by using a getter filter. The custom-made injector designed to focus the ammonia flux onto the substrate was used for the gas delivery, while aluminum and gallium were provided via conventional effusion cells. The growth sequence to minimize the residual ammonia and subsequent memory effects is the following: (1) Native oxides are desorbed at $750^{\circ}C$ (Fig. (a) for [$1^-10$] and [001] azimuth) (2) 40 nm thick AlN is first grown using nitrogen rf plasma source at $900^{\circ}C$ nder the optimized condition to maintain the layer by layer growth of AlN buffer layer and slightly Al-rich condition. (Fig. (b)) (3) After switching to ammonia source, GaN growth is initiated with different V/III ratio and temperature conditions. A streaky RHEED pattern with an appearance of a weak ($2{\times}2$) reconstruction characteristic of Ga-polarity is observed all along the growth of subsequent GaN layer under optimized conditions. (Fig. (c)) The structural properties as well as dislocation densities as a function of growth conditions have been investigated using symmetrical and asymmetrical x-ray rocking curves. The electrical characteristics as a function of buffer and GaN layer growth conditions as well as the growth sequence will be also discussed. Figure: (a) RHEED pattern after oxide desorption (b) after 40 nm thick AlN growth using nitrogen rf plasma source and (c) after 600 nm thick GaN growth using ammonia source for (upper) [110] and (lower) [001] azimuth.

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