• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 Grop Science and Biotechnology in the 21st Century
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• pp.31.1-31.1
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• 2006

• 원예과학기술지
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• 제28권6호
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• pp.1039-1050
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• 2010

당근은 서양뿐만 아니라 중국 및 한국과 같은 아시아 전역에서 요리로 많이 이용되는 유용한 작물 중 하나이다. 그러나 당근 종자 표면에는 모(毛)가 존재하고 이 종모는 발아율을 증가시키기 위해 제거해야 한다. 더욱이 종모 처리는 시간과 인력 및 자본의 소비와 같은 추가적인 손실을 동반하였다. 이러한 문제점을 방지하기 위해 단모종자를 이용하여 모형성과 관련된 유전자의 연구가 필요하다. 당근 종모의 발달은 2차 세포벽의 합성단계 동안 cellulose의 합성 과정과 연관되어 있음을 바탕으로, EST profiling을 통해 종모와 관련된 유전자를 탐색하고자 하였다. 당근 종모 형성에 관련된 유전자 발현을 조사하기 위해, 성숙 초기 단계의 단모종자 659-1개체와 유모종자 677-14개체를 이용하여 cDNA library를 구축하였다. 단모종자 659-1개체와 유모종자 677-14개체에서 확보된 EST 염기서열의 NCBI database BLASTX 분석을 통한 EST profiling 결과, 172개와 224개의 unigene은 이미 알려진 단백질 염기서열과 상동성을 보였으며 나머지 233개와 192개의 unigene은 확인되지 않는 유전자들이었다. EST는 추정되는 기능에 따라 16개의 category로 그룹화되었다. 전체 EST 중 29개의 unigene이 2차 세포벽 합성 단계 동안 cellulose의 합성 pathway상의 종모 형성을 조절하는 유전자로 추정되며, 실제로 종모 발달과 관련된 14개의 unigene이 유모종자 계통에서만 발견되었다.

• 농업과학연구
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• 제46권4호
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• pp.971-980
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• 2019

We profiled the health-promoting bioactive components in nine types of soybean seeds with different seed coat colors (yellow, green, brown, and black) and investigated the effects of different extraction solvents (methanol, ethanol, and water) on their antioxidant activities. The carotenoid and anthocyanin compositions varied greatly by seed color, and the phenolic acids, total phenol, and total flavonoid contents differed by genotype. The carotenoid content was relatively higher in soybean seeds with green and black seed coats than in those with a yellow seed coat while lutein was the most plentiful. The anthocyanin content was considerably higher in the soybean seed with the black seed coat. The results of the DPPH assay showed strong antioxidative activities in the methanol- and water-extracts compared to the ethanol-extract, irrespective of the seed coat colors. Moreover, the soybean seeds with the black seed coat exhibited the highest antioxidant activity among the samples, regardless of the extraction solvent used. Eighteen bioactive compounds were subjected to data-mining processes including principal component analysis and hierarchical clustering analysis. Multivariate analyses showed that brown and black seeds were distinct from the yellow and green seeds in terms of the levels of carotenoids and anthocyanins, respectively. These results help our understanding of the compositional differences in the bioactive components among soybean seeds of various colors, providing valuable information for future breeding programs that seek to enhance the levels of compounds with health benefits.

• 한국작물학회지
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• 제54권4호
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• pp.346-350
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• 2009

The cultivated radish (Raphanus sativus L.) is a major vegetable crop in the world wide and fast-growing species that grows inhabitats of six continents. It is very important to determine hybrid seed purity in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in $F_2$-hybrid radish cultivars demonstrated. One hundred eighty seeds from the F1 male and female harvest were subsequently screened for seed purity using 13 primers. The 13 primers result in 17 cultivar-specific bands and 23 variable RAPD bands scored for cultivar. RAPD analysis of hybrid seeds from the harvest revealed 128 seeds tested except underdevelopment and decayed seeds were sibs. Especially, $F_2$ hybrids of radish, OPC13, OPD20 were presented clear hybrid bands. It maintains higher than average level of genetic diversity compared with their correspondent parents. RAPD amplification of DNA extracted from germinated individuals from the female harvest reveal that 10 of 208 seeds tested were self-inbred (4.8%). RAPD analysis of hybrid seeds from the male harvest revealed 7 of the 208 seeds tested were sibs (3.4%). The RAPD may lead to a better insight in to the hybrid seed purity.

• KIEAE Journal
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• 제16권6호
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• pp.29-38
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• 2016

Purpose: This study is conducted to contribute to the improvement of Green Standard for Energy and Environmental Design(G-SEED) from the existing energy physical efficiency-centric system to the well-being-centric green building certification system. Method: In order to modify existing G-SEED Certification, three phases of research have been conducted; 1) identification of needs of well-being-centric environment-friendly construction certification system, 2) comparison of domestic certification system with WELL Building standard(WELL) which focuses on human health and promotion of wellness and 3) AHP analysis to sort out the supplement items from WELL. Result: We proposed four alternatives which should be preferentially introduced to the existing domestic certification system; addition of independent 'well-being' section(field) in G-SEED, insertion of 'well-being' indicators in each relevant section of G-SEED, addition of 'well-being' indicators in 'Innovative Design(ID)' of G-SEED and independent score systems for 25 items in a high priority.

• 한국작물학회지
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• 제45권4호
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• pp.227-231
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• 2000

Small seed size is one of the major traits of soybean cultivars for sprouts with regard to high sprout yield. This study was conducted to identify quantitative trait loci (QTL) for seed size and weight in a set of F 6 seeds of 89 lines derived from a cross between 'Pureunkong', a soybean cultivar developed for sprouts and 'Jinpumkong 2', a soybean cultivar with no beany taste in seed due to the lack of lipoxygenases. The genetic map of 25 linkage groups with a total of 98 markers including RFLP, RAPD, SSR and classical markers was constructed from this F/sbu 5/-derived population and was used for QTL analysis. 'Pureunkong' was significantly smaller (P＜0.01) than 'Jinpumkong 2' in seed size and seed weight. Genetic variation was detected and transgressive segregation was common in the population for these traits. Seven DNA markers including opT14-1600 in LG A2, opF02-400 in LG B2, Satt100, opC09-700, opG04-730 and opQll-650 in LG C2, and opY07-1100 ＆ 1000 in LG(unknown) were significantly associated and accounted for 4.7 to 10.9% and 5.1 to 10.1 % of the phenotypic variation in seed size and seed weight, respectively. 'Pureunkong' alleles increased seed size and seed weight at the all four significant marker loci on the LG C2. These marker loci in LG C2 were closely linked and were presumed to be a single QTL. Overall, at least three independent QTLs from 3 linkage groups (A2, B2, and C2) were putatively involved in the control of seed size and seed weight.

• 한국정보보호학회:학술대회논문집
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• 한국정보보호학회 2003년도 동계학술대회
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• pp.39-44
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• 2003

오류분석 공격은 암호시스템에 오류를 주입한 후 그 출력 결과를 분석하여 비밀키를 찾아내는 물리적 공격 방법으로서 RSA, ECC를 포함한 공개 키 시스템을 비롯하여 DES, AES와 같은 대칭 키 암호시스템에도 공격이 시도되고 있다. 본 논문에서는 기존 DES 공격에 사용된 오류 주입의 가정만 있으면 국내 표준 블록 암호 알고리듬인 SEED 역시 오류 주입 공격이 가능함을 증명한다. 또한, 오류 주입 공격에 의해 SEED의 라운드 키 두개만 공격되면 원 암호 키가 모두 노출될 수 있음을 검증한다.

• Journal of Plant Biotechnology
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• 제39권4호
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• pp.242-252
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• 2012

Seed storage oils are essential resources for not only human and animal diets but also industrial applications. The primary goal of this study was to increase seed oil content through comparative analysis of two seed-specific promoters, AtFAE1 from Arabidopsis Fatty Acid Elongase 1 gene and BnNapin from Brassica napus seed storage protein gene. AtWRI1 and AtDGAT1 genes encoding an AP2-type transcription factor and a Diacylglycerol Acyltransferase 1 enzyme, respectively, were expressed under the control of AtFAE1 and BnNapin promoters in Arabidopsis. The total seed oil content in all transgenic plants was increased by 8-11% compared with wild-type seeds. The increased level of oil content in AtWRI1 and AtDGAT1 transgenic lines under the control of both promoters was similar, although the activity of the BnNapin promoter is much stronger than that of AtFAE1 promoter in the mature stage of developing seeds where storage oil biosynthesis occurs at a maximum rate. This result demonstrates that the AtFAE1 promoter as well as the BnNapin promoter can be used to increase the seed oil content in transgenic plants.

• 한국작물학회지
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• 제47권
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• pp.200-215
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• 2002

There are some international standards and organization controling the quality characteristics of varieties and seeds such as UPOV, OECD, ISTA, AOSA. The National List system to check variety performance such as quality characteristics of plant varieties and seeds was established by each countries. The UPOV (International Union for the Protection of New varieties of Plant) regulates the requirement of plant variety protection that is distinctness (D), uniformity (U), stability (S) (here in after called DUS), Novelty and Denomination, and some quality characteristics are used to examine the requirement of plant variety protection by each crops. OECD (Organization for Economic Cooperation Development) seed scheme regulates the seed certification for seed trade between countries. ISTA (International Seed Testing Association) and AOSA(Association for Official Seed Analyst) regulates the seed analysis standard and methods. Most of the countries in Europe has the National List system, which check the value for cultivation and use(VCU), that is to say, variety performance such as yield, quality and stress to environment. All the seeds should be enlisted in the National List before sell the seed in their country. All the quality characteristics checked variety performance are for instance, amylose and amylopectin content in rice, molting quality, $\beta$-glucan, protein in barley, protein and sugar content in soybean, sugar and amylopectin content in corn etc. Conditions for the protection of new variety of plant are DUS. Quality characteristics may be the important characteristics and used to check DUS in crop by crop. It is very important to develop a new characteristics and establish standard method fur examine the VCU and DUS test for each crop.

• 대한건축학회논문집:구조계
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• 제34권5호
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• pp.59-67
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• 2018

G-SEED's evaluation of existing buildings is an important point because the energy consumption and CO2 emissions of existing buildings are continuously increasing due to the aging of buildings over time. However, as G-SEED certification is concentrated on new buildings and the standards are not yet available, we will make improvements to evaluate the existing buildings of G-SEED. In order to improve G-SEED for EB, two research procedures were carried out. First, LEED EBOM and LEED for NC compare and analyze evaluation items and methods, and LEED EBOM focuses on evaluation of existing buildings. Second, we analyzed the certification evaluation contents of eight domestic LEED EBOM accredited buildings and obtained items that can be applied or need to be applied when revising G-SEED for EB. LEED EBOM is focused on improving the performance and utilization of data that track and manage the energy and environmental effects of building operations. As a result of the case analysis, the evaluation of G-SEED for EB requires quantitative evaluation items such as environmental management of buildings, energy performance status of building, improvement plan and improvement of performance according to tracking value. Although G-SEED for EB is not structured yet and there are few cases of certification, it is expected that quantitative evaluation of existing buildings will be made through continuous research.