• Title/Summary/Keyword: seed analysis

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A Study on the Domestic Appllication of the Concept of Seed Transfer Zone in the U.S (미국 잠정종자이동구역(Seed transfer zone) 개념의 국내 적용 방안)

  • Kim, Chae-Young;Kim, Whee-Moon;Song, Won-Kyong;Choi, Jae-Yong
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.24 no.2
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    • pp.39-56
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    • 2021
  • The seed zone is a map that describes the areas where plant material can be transferred with little risk for properly adapting to a new location. The seed zone study is largely divided into studies based on genetic data and studies based on climatic data. Can be. This study was conducted to establish a temporary domestic seed zone applicable to the entire Korean Peninsula and evaluate its possibility based on the US climate-based seed zone establishment methodology. The temporary seed zone was constructed in the same way as the US case by superimposing the data obtained by dividing the winter minimum temperature into 12 grades and the data obtained by dividing the annual heat: moisture index into 6 grades. As a result of the analysis, 65 temporary seed zones were formed throughout the Korean Peninsula, and the areas of the seed zones representing the smallest and largest areas were 3.0km2 and 29,423.0km2, respectively, and it was confirmed that they had an average size of about 5,064.9km2. Temporary seed zones applied in Korea show a pattern of changes in temperature according to the relatively horizontal forest zone, and it was confirmed that the area where the Baekdu-daegan ecological axis is located has a tendency to show lower dryness than other areas. This study applied the US climate-based seed zone methodology in Korea as a pilot, and confirmed the climatic similarity across the Korean Peninsula. Furthermore, it is expected to provide an optimal seed map that improves the success rate of restoration in the future by revising the seed zone grade suitable for the domestic environment in consideration of the results of this study and the possibility of seed adaptation to the field survey and environmental space.

The Variation of Cone, Seed and Germination Characteristics of Picea jezoensis (Siebold & Zuccarini) Carriere Populations in Korea (한국산(韓國産) 가문비나무 자생집단(自生集團)의 구과(毬果), 종자(種子) 및 발아특성(發芽特性) 변이(變異))

  • Song, Jeong-Ho;Jang, Kyung-Hwan;Kim, Du-Hyun;Lim, Hyo-In
    • Korean Journal of Plant Resources
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    • v.24 no.1
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    • pp.69-75
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    • 2011
  • This study was conducted to investigate the variation of cone, seed and germination characteristics among populations and among individuals within populations of Picea jezoensis(Siebold & Zuccarini) Carriere distributed in Korea. Cone collected from 25 trees in two natural populations(Mt. Jiri and Mt. Dokyu) and their thirteen cone and seed characteristics as well as three germination behaviors were analyzed. Statistical analysis showed significant differences among populations and among individuals within populations in 9 traits except for seed breadth, seed weight, index of seed wing and mean germination time. Generally, morphological variation of cone and seed wings from Mt. Jiri population has smaller and longer than those of Mt. Dokyu population. Percentage of sound seeds and Tetrazolium test from Mt. Jiri population showed 1.79 and 1.87 higher values than Mt. Dokyu population, respectively. The maximum germination percentage was obtained at the optimum temperature of $20^{\circ}C$ and in this case, mean germination time and germination rate showed 7.5 days and 2.9 ea./day, respectively. In seed germination behaviors, percentage(40.7%) and rate(0.90 ea./day) of germination in Mt. Jiri population were more higher and faster than those of Mt. Dokyu population(17.7%, 0.37 ea./day). According to correlation analysis, P. jezoensis populations with small restricted distribution may have been reduced because seed qualities were correlated with increased levels of inbreeding and disproportion flowering.

The Effect of Cone Protective Net and the Morphological Variation of Cone and Seed of Korea Rare Endemic Pinus pumila Regel (희귀수종 눈잣나무의 구과보호망 효과 및 구과와 종자의 형태적 변이)

  • Song, Jeong-Ho;Lim, Hyo-In;Jang, Kyung-Hwan;Kim, Du-Hyun;Son, Jang-Ick
    • Korean Journal of Plant Resources
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    • v.25 no.4
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    • pp.401-406
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    • 2012
  • This study was conducted to investigate the effect of cone protective net and the variation of cone and seed characteristics of a unique and rare dwarf stone pine (Pinus pumila Regel) population in Mt. Seorak which is in the southern peripheral of South Korea. Iron protective net (50 mesh, 25 ${\times}$ 25 cm) was effective in reducing birds and rodents damage to conelet, thereby it was possible to safely collect seed genetic resources. Early July was observed to be the most appropriate season to cover conelet with Iron protective net. P. pumila have ovoid-triangle shaped, wingless seeds and indehiscent cones, seeds dissemination is mainly by the hoarding behavior of nutcrackers and rodents. Statistical analysis showed that there were significant differences among individuals within population, and the mean characteristics of the species were 35.3 mm, 25.6 mm, 1.38, 6.6 g and 39.3 ea in the cone length, cone width, cone index, cone weight and in number of seeds per cone, respectively. Coefficients of variations in seed weight and number of seeds per cone were relatively high (21.7%, 21.5%, respectively) compared to other traits. Based on the correlation analysis between cone characteristics and seed characteristics, the length of cone and seed showed a significant positive correlation with the seed size and the seed weight, but the width and thickness of seed showed a negative correlation with the number of seeds per cone.

Identification of the quantitative trait loci (QTL) for seed protein and oil content in soybean.

  • Jeong, Namhee;Park, Soo-Kwon;Ok, Hyun-Choong;Kim, Dool-Yi;Kim, Jae-Hyun;Choi, Man-Soo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.148-148
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    • 2017
  • Soybean is an important economical resource of protein and oil for human and animals. The genetic basis of seed protein and oil content has been separately characterized in soybean. However, the genetic relationship between seed protein and oil content remains to be elucidated. In this study, we used a combined analysis of phenotypic correlation and linkage mapping to dissect the relationship between seed protein and oil content. A $F_{10:11}$ RIL population containing 222 lines, derived from the cross between two Korean soybean cultivars Seadanbaek as female and Neulchan as male parent, were used in this experiment. Soybean seed analyzed were harvested in three different experimental environments. A genetic linkage map was constructed with 180K SoyaSNP Chip and QTLs of both traits were analyzed using the software QTL IciMapping. QTL analyses for seed protein and oil content were conducted by composite interval mapping across a genome wide genetic map. This study detected four major QTL for oil content located in chromosome 10, 13, 15 and 16 that explained 13.2-19.8% of the phenotypic variation. In addition, 3 major QTL for protein content were detected in chromosome 10, 11 and 16 that explained 40.8~53.2% of the phenotypic variation. A major QTLs was found to be associated with both seed protein and oil content. A major QTL were mapped to soybean chromosomes 16, which were designated qHPO16. These loci have not been previously reported. Our results reveal a signi cant genetic relationship between seed protein and oil fi content traits. The markers linked closely to these major QTLs may be used for selection of soybean varieties with improved seed protein and oil content.

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Contrasting rice sub-populations to tocols ratio associated with seed longevity

  • Lee, Jae-Sung;Kwak, Jieun;Yoon, Mi-Ra;Lee, Jeom-Sig;Hay, Fiona R.
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.31-31
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    • 2017
  • Understanding the mechanism(s) to overcome or prevent seed ageing deterioration during storage is of fundamental interest to seed physiologists. Vitamin E (tocols) is known as a key metabolite to efficiently scavenge lipid peroxy radicals which cause membrane breakdown resulting in seed ageing. However, in rice research this hypothesis has been tested for very few lines only without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing aus, indica, temperate japonica and tropical japonica subpopulations, were used for tocols analysis (quantification of ${\alpha}$-, ${\beta}$-, ${\gamma}$-, ${\delta}$-tocopherol/tocotrienol by ultra performance liquid chromatography) and storage experiments at $45^{\circ}C$ and 10.9% seed moisture content (sample taken for germination testing every 3 days up to 60 days). To examine interactions between DNA sequences and phenotype, the 700k high-density single-nucleotide polymorphism marker data-set was utilized. Both seed longevity (time for viability to fall to 50%; $p_{50}$) and tocols content varied across subpopulations due to heterogeneity in the genetic architecture. Among eight types of tocol homologues, ${\alpha}$-tocopherol and ${\gamma}$-tocotrienol were significantly correlated with $p_{50}$ (negatively and positively, respectively). While temperate japonica varieties were most abundant in ${\alpha}$-tocopherol, indica varieties recorded 1.3 to 1.7-fold higher ${\gamma}$-tocotrienol than those of other subpopulations. It was highlighted that specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism.

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A Study on the Design of Key Scheduler Block Cryptosystem using PRN (PRN을 이용한 키 스케줄러 블록암호시스템 설계에 관한 연구)

  • 김종협;김환용
    • Journal of the Korea Society of Computer and Information
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    • v.8 no.2
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    • pp.112-121
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    • 2003
  • Information Protection and cryptography technology is developed with if but solved problem of real time processing and secret maintain. Therefore this paper is Proposed new PRN-SEED(Pseudo-Random Number-SEED) for the increasing secret rate and processing rate perform performance analysis with existed other cryptography algorithms. Proposed new PRN-SEED crypto-algorithm increase in the processing rate than existed algorithms use bit and byte mixed operation with RNG(Random Number Generator). PRN-SEED that performs simultaneous operations have higher 1.03 in the processing rate and 2 in the cryptosystem performance than existed cryptosystems. Implementation for PRN-SEED use Synopsys Design Analyser Ver. 1999.10, samsung KG75 library and Synopsys VHDL Debegger. As a simulation result, symmetric cryptosystem DES operate 416Mbps at the 40MHz and Rijndael operate 612Mbps at the 50MHz. PRN-SEED cryptosystem have gate counting 10K and operate 430Mbps at the 40MHz and 630Mbps at the 50MHz.

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Morphological Characterization of Fagopyrum esculentum Germplasm for Rutin and Quercetin Contents

  • Rauf, Muhammad;Choi, Yu Mi;Lee, Sukyeung;Hyun, Do Yoon;Lee, Myung-Chul;Oh, Sejong;Yoon, Hyemyeong
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.52-52
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    • 2019
  • Buckwheat is well-known crop for containing a high contents of flavonoids that are effective in vascular disease. The current study was performed to estimate the influence of morphological characterization of Fagopyrum esculentum (ES) germplasm for seed's two major flavonoids contents: rutin and quercetin. We found that the red stem color, pale green leaf color, arrowhead leaf shape, white flower color, pale brown seed coat color, and egg-shaped seed were significantly associated with 77%, 56.7%, 83.7%, 98.7%, 70.8% and 74.5% germplasm, respectively. Overall, the rutin contents of ES germplasm ranged from 0.30 to 47.86 mg/100g dry weight (DW) and the quercetin contents ranged from 0 to 1.22 mg/100g DW. The rutin contents of germplasm possessing red stem color, pale green leaf color, arrowhead leaves, white flower color, pale brown seed coat color and egg-shaped seed ranged from 7.22 to 47.86 mg/100g DW. However, the quercetin contents of germplasm with red stem color and pale brown seed coat color ranged from 0 to 1.15 mg/100g DW, with pale green leaves ranged from 0 to 0.96 mg/100g, with arrowhead leaves and white flower ranged from 0 to 1.22 mg/100g and with egg-shaped seed ranged from 0.32 to 1.22 mg/100g DW. In PCA analysis, the first three principal components (PCs) showed Eigen value more than 1 and accounted for 51.70% of variation. For both higher contents of rutin and quercetin, the morphological evaluation in ES shows a tendency of red stem color, arrowhead leaves, pale green leaf color, white flower color, pale brown seed coat color and egg-shaped seed. From this information, we can assume the rutin and quercetin contents by the morphological characteristics of the germplasm. And It could be useful in improving the rutin and quercetin contents and selecting proper resources for cultivation in existing buckwheat cultivars.

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Genetic Analysis of Seed Size in Watermelon (수박 종자크기에 대한 유전분석)

  • Kim, Yong-Jae;Yang, Tae-Jin;Park, Young-Hoon;Lee, Yong-Jik;Kang, Sun-Cheol;Kim, Yong-Kwon;Cho, Jeoung-Lai
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.412-419
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    • 2009
  • In order to study the inheritance of watermelon seed size, we used six watermelon lines of different seed sizes as parental lines. Six lines include three accessions, 'PI525088' with giant seed (GS), 'Charleston Gray' with big seed (BS), and 'NT' with normal medium size seed (NS), and three near isogenic lines, 'NTss' with small seed (SS), 'NTms' with micro seed (MS) and 'NTts' with tomato seed size (TS) bred by crosses between accession 'NT' of normal seed size and accession 'TDR' of the smallest seed size,. We inspected $F_1$, $F_2$, $BC_1F_1$ (P1), $BC_1F_1$ (P2) populations from the crosses between the adjacent seed size materials like $GS{\times}BS$, $BS{\times}NS$, $NS{\times}SS$, and $MS{\times}TS$, and two crosses between parental lines showing relatively big difference in seed size such as $GS{\times}TS$ and $NS{\times}TS$. Partial single dominant inheritance patterns were observed between $GS{\times}BS$, $NS{\times}SS$, and $MS{\times}TS$ and inheritance patterns based on two genes or more than two genes were speculated between $BS{\times}NS$. A very wide segregation range was observed from the population of $GS{\times}TS$ indicating many quantitative genes involved in the seed sizes. Overall, we speculated that more than six genes are involved in between the biggest and smallest seed size watermelon and three major genes between the normal seed size and the smallest seed size watermelon.

The Effects of Grape Seed Flour on the Quality of Turkish Dry Fermented Sausage (Sucuk) during Ripening and Refrigerated Storage

  • Kurt, Sukru
    • Food Science of Animal Resources
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    • v.36 no.3
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    • pp.300-308
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    • 2016
  • In this study, the effects of grape seed flour on the physical-chemical properties, microbiological and sensory properties of Turkish dry fermented sausage, sucuk, was investigated. After the sausages produced with beef, beef fat, sheep tail fat and spices, they were ripened for 14 d. Then they were vacuum-packaged and stored for 80 d at 4℃. The effects of grape seed flour (GSF; 0%, 0.75%, 1.5%, 3%) on the physical-chemical properties (pH, moisture, fat, protein, free fatty acids, thiobarbituric acids, diameter reduction, ripening yield, instrumental colour), microbiological properties (total aerobic mesophilic and lactic acid bacteria, Enterobacteriaceae, mould and yeast) and sensory properties of the sausages were investigated. Grape seed flour decreased moisture, TBA, diameter reduction, instrumental colour (a, b) values and sensory analysis scores during the ripening period; it also decreased TBA, instrumental colour (L, a, b) values, total aerobic mesophilic and lactic acid bacteria counts during the storage period. It was concluded that grape seed flour has a potential application as an additive in dry fermented sausages.

The Analysis of Soil Seed Bank at Major Wetlands in Nakdong River Basin and Central Korea (낙동강 일대와 중부 지역 주요 습지의 토양종자은행 분석)

  • Ju, Eun Jeong;Kim, Jae Geun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.12 no.5
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    • pp.77-91
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    • 2009
  • The purpose of this study is to define the species composition of the soil seed banks at major wetlands in Nakdong river basin and central korea and to investigate how soil seed banks relate to aboveground vegetation and soil texture. In 2005, we sampled seed banks at Baksil reservoir, Jillal marsh, Deapyeng marsh, Hwapo marsh, Upo marsh and Junam reservoir in Nakdong river basin and Osan stream and Amsa-dong in Seoul. The soil seeds were estimated with the emergence method from April to October. Total numbers of species at the seed banks were 33 at Baksil, 18 at Daepyeong, 35 at Jillal, 56 at Upo, 32 at Hwapo, 47 at Osan stream, 54 at Amsa waterside, 31 at Amsa meadow. The species diversity in the soil seed banks of Upo marsh was the highest as 0.95. The community overlap index that compares aboveground with underground vegetation is high in the Upo marsh (0.34), Jilla marsh (0.36), and Osan stream (0.27). Soil texture affected distribution of 8 species, Lindernia procumbens, L. attenuata, Arenaria serpyllifolia, Juncus effusus, Persicaria thunbergii, Eragrostis multicaulis, Cyperus nipponicus, Scirpus fluviatilis. Considering the social and cultural values, soils at Amsa meadow, Hwapo marsh and Osan stream have worth to use for wetland restoration.