• International Journal of High-Rise Buildings
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• v.5 no.4
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• pp.263-270
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• 2016

In this paper, the stability requirements for diagrid and mega braced structures are examined. The role of the secondary bracing system for the stability of a diagrid structure is discussed. A simple procedure is proposed for the design of the secondary bracing system when it is required. As a case study, the design of the Hearst Tower diagrid and its secondary bracing system are presented.

• Journal of Internet of Things and Convergence
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• v.7 no.4
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• pp.15-21
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• 2021

This study aims to derive the problems of firefighting and safety measures for architectural heritage with a wooden structure in rural areas and present their improvement measures. To identify those problems, this study grasped the features of the cultural heritage through the building structure and environment of a wooden Catholic secondary station in Dangjin, and analyzed fires that may occur and safety factors. As a result, although the mission station is an important cultural property in terms of its history, place and local identity, it had problems with disaster prevention systems such as vulnerable safety including fire and difficulties in fire recognition and initial firefighting. Therefore, this study concluded through its review and analysis that a disaster prevention system such as stronger firefighting is needed; that fire fighting facilities suitable for the characteristics of the secondary station with a wooden structure should be installed and a main player should be arranged in fire prevention activities to improve the fire prevention system of the cultural property; and that as most mission stations are located in rural areas, it is necessary to more thoroughly protect wooden-structure secondary stations from natural disasters such as forest fire and to improve fire response measures.

• Journal of the Korean Magnetic Resonance Society
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• v.20 no.2
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• pp.50-55
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• 2016

Backbone $^1H$, $^{13}C$ and $^{15}N$ resonance assignments are presented for the anticodon binding domain (residues 557-674) of human glycyl-tRNA synthetase (GRS). Role of the anticodon binding domain (ABD) of GRS as an anticancer ligand has recently been reported and its role in other diseases like Charcot-Marie-Tooth (CMT) and polymyositis have increased its interest. NMR assignments were completed using the isotope [$^{13}C/^{15}N$]-enriched protein and chemical shifts based secondary structure analysis with TALOS+ demonstrate similar secondary structure as reported in X-ray structure PDB 2ZT8, except some C-terminal residues. NMR signals from the N-terminal residues 557 to 571 and 590 to 614 showed very weak or no signals exhibiting dynamics or conformational exchange in NMR timescale.

• BMB Reports
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• v.40 no.2
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• pp.232-238
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• 2007

The p13 gene is uniquely present in Group II nucleopolyhedroviruses (NPVs) and some granuloviruses, but not in Group I NPVs. p13 gene was first described by our laboratory in Leucania separatamultiple nuclear polyhedrosis virus (Ls-p13) in 1995. However, the functions of Ls-P13 and of its homologues are unknown. When Ls-p13 was inserted into Autographa californica nucleopolyhedrovirus, a Group I NPV, polyhedra yield was inhibited. However, this inhibition was prevented when the leucine zipper-like domain of Ls-p13 was mutated. To determine the cause of this marked difference between Ls-P13 and leucine zipper mutated Ls-P13 (Ls-P13mL), oligomerization and secondary structure analyses were performed. High performance liquid chromatography and yeast two-hybrid assays indicated that neither Ls-P13 nor Ls-P13mL could form oligomers. Informatics and circular dichroism spectropolarimetry results further indicated marked secondary structural differences between Ls-P13 and Ls-P13mL. The LZLD of Ls-P13 has two extended heptad repeat units which form a hydrophobic surface, but it is short of a third hydrophobic heptad repeat unit for oligomerization. However, the mutated LZLD of Ls-P13mL lacks the above hydrophobic surface, and its secondary structure is markedly different. This difference in its secondary structure may explain why Ls-P13mL is unable to inhibit polyhedra yield.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1991.10a
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• pp.63-68
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• 1991

The frequency window method has been extended to include strong coupling and multiple connections between the primary structure and the secondary structures. The rational polynomial expansion of the eigenvalue problem and the analytical methods for its solution are novel and distinguish this work from other eigenvalue analysis methods. The key results are the identification of parameters which quantify the resonance and coupling characteristics; the derivation of analytical dosed-form expressions describing the fundamental modal properties of the frequency windows; and the development of an iterative procedure which yields accurate convergent results for strongly-coupled primary-secondary structures.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.2
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• pp.73-76
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• 2007

A large number of natural products were worldwidely discovered from maine organisms. There are many secondary metabolites produced by maine organisms which showed very strong bio-activities and distinct bio-active mechanisms. In korea, about 350 secondary metabolites including more than 280 novel compounds have been isolated and structually defined. Many researches for developing products such as cosmetics, functional food materials, anti-fouling substances are being performed using distinct activities of marine organisms. Although the research on marine natural products has been remained at its initial or developing stage of drug development, significant progress has been made for isolation, structure determination and bioassay of secondary metabolites.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.65-65
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• 2003

After many genome projects, algorithms and software to process explosively growing biological information have been developed. To process huge amount of biological information, high performance computing equipments are essential. If we use the remote resources such as computing power, storages etc., through a Grid to share the resources in the Internet environment, we will be able to obtain great efficiency to process data at a low cost. Here we present the performance improvement of the protein secondary structure prediction (PSIPred) by using the Grid platform, distributing protein sequence data on the Grid where each computer node analyzes its own part of protein sequence data to speed up the structure prediction. On the Grid, genome scale secondary structure prediction for Mycoplasma genitalium, Escherichia coli, Helicobacter pylori, Saccharomyces cerevisiae and Caenorhabditis slogans were performed and analyzed by a statistical way to show the protein structural deviation and comparison between the genomes. Experimental results show that the Grid is a viable platform to speed up the protein structure prediction and from the predicted structures.

• Journal of KSNVE
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• v.7 no.4
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• pp.655-661
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• 1997

Base-isolation of a primary structure generally decrete the seismic response of its own and the secondary structure. It may cause an adverse effect on the seismic response of secondary system when the system is submerged and subject to a considerable hydrodynamic effect. In this paper, it is shown how, and how much, the base isolation of the primary structure can affect the secondary system response in extreme cases through dynamic analysis of a simplified coupled model for a submerged secondary system and a base-isolated primary structure. As an aseismatic design approach to reduce the response of the submerged system, optimization of the fluid gap, which controls the hydrodynamic mass effect, is performed. As an alternative approach in case where the control of fluid gap is unrealistic, application of base isolation to the submerged system is suggested. Effectiveness of various combinations of the primary base and secondary base isolations are compared.

• Genomics & Informatics
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• v.20 no.4
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• pp.41.1-41.9
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• 2022

The pathogen Gallibacterium anatis has caused heavy economic losses for commercial poultry farms around the world. However, despite its importance, the functions of its hypothetical proteins (HPs) have been poorly characterized. The present study analyzed the functions and structures of HPs obtained from Gallibacterium anatis (NCTC11413) using various bioinformatics tools. Initially, all the functions of HPs were predicted using the VICMpred tool, and the physicochemical properties of the identified virulence proteins were then analyzed using Expasy's ProtParam server. A virulence protein (WP_013745346.1) that can act as a potential drug target was further analyzed for its secondary structure, followed by homology modeling and three-dimensional (3D) structure determination using the Swiss-Model and Phyre2 servers. The quality assessment and validation of the 3D model were conducted using ERRAT, Verify3D, and PROCHECK programs. The functional and phylogenetic analysis was conducted using ProFunc, STRING, KEGG servers, and MEGA software. The bioinformatics analysis revealed 201 HPs related to cellular processes (n = 119), metabolism (n = 61), virulence (n = 11), and information/storage molecules (n = 10). Among the virulence proteins, three were detected as drug targets and six as vaccine targets. The characterized virulence protein WP_013745346.1 is proven to be stable, a drug target, and an enzyme related to the citrate cycle in the present pathogen. This enzyme was also found to facilitate other metabolic pathways, the biosynthesis of secondary metabolites, and the biosynthesis of amino acids.

• Journal of Power Electronics
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• v.7 no.4
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• pp.301-309
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• 2007

The flyback converter is one of the most attractive isolated converters in small power applications because of its simple structure. However, it suffers from high device stress, large transformer size, and high voltage stress across its switch and diode. To solve these problems a new cost-effective PWM single-switch isolated converter is proposed. The proposed converter has no output filter inductor, reduced voltage stress on the secondary devices, and reduced transformer size. Moreover, the switch turn-off loss is reduced and no dissipative snubber across the secondary diode is required. Therefore, it features a simple structure, a low cost, and high efficiency. The operational principle and characteristics of the proposed converter are presented and compared with the flyback converter and then verified experimentally.