• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.1
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• pp.73-77
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• 2005

Plant-derived natural products have been and will continue to be valuable sources. Elicitors have been employed to modify cell metabolism in order to enhance the productivity of useful metabolites in plant cell/tissue cultures. In this study, several elicitors were used to improve the productivity of useful metabolites and to reduce culture time for archiving high concentration in P. ginseng hairy root cultures. The addition of chitosan, chitosan oligosaccharide and alginate oligosaccharide to the culture of P. ginseng hairy roots caused growth to be inhibited with the increase in elicitor concentration. The usage of the chitosan elicitor and D-glucosamine caused a slight decrease in hairy root growth, whereas total ginseng saponin accumulated slightly with the increase in elicitor concentration. When gel beads were added to the culture medium at the initial period, hairy root growth was enhanced. The maximum growth was 1.35 times higher than that of the control at $1\%$ (w/v). Total ginseng saponin content decreased due to the addition of alginate beads. This would result in consistent diffusion of lower levels of calcium ions during the culture period that promotes biomass growth.

• Journal of Microbiology and Biotechnology
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• v.25 no.4
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• pp.452-458
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• 2015

The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35℃, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12-ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicum-based biocatalyst.

• KSBB Journal
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• v.9 no.2
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• pp.180-185
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• 1994

The productivity of alkaloid in the airlift fermentor operation was less than that of suspension coltures of Eschscholtzia californica cells in the shake flask. To overcome the productivity reduction, a gas recycle airlift fermentor was developed because the gas-stripping in normal airlift fermentor was believed to play a significant role for productivity reduction. The alkaloid content in the gas recycle system with Eschscholtzia californica suspension cells was 2.7 times higher than that of normal airlift fermentor. The productivity of alkaloids and $CO_2$ concentration were affected by the volume of gas reservoir in the gas recycle airlift fermentor.

• The Korean Journal of Ecology
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• v.19 no.4
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• pp.305-320
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• 1996

It is necessary to obtain information about the productivity of the human-influenced forest and to understand the consumption of biomass resources in secondary forest in order to examine the resource flux by human activity in rural landscape. Thus the aims of this study were to elucidate the biomass and their use of secondary Pinus densiflora forests and to discuss sustainable utilization of secondary forests in rural landscape system. This study was carried out in Yanghwa-ri, Kongjugun, Chungcheongnam-do, central Korea. The changes of growth rate and aboveground biomass of a pine forest for 2 years were analyzed to understand forest management regimes in rural pine forests. Through allometric equations deduced from 25 sample trees, biomass was estimated. The biomass increase of pine forest was approximately 16.36 t/ha/yr in the unexploited stand and 12.24 t/ha/yr in the exploited stand. These were nearly equal to those of natural pine forests in central Korea. This result proved that human-influenced pine forest in rural landscape as well as the natural one has high potentiality to provide forest products. Making graveyard in forest-land was the important disturbance and land-use which currently occurring in rural landscape in the study area. Finally, we presented some forest management for stutainable and positive uses of secondary forests as one of the local energy resources in terms of the holistic landscape-ecological view.

• Plant Biotechnology Reports
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• v.5 no.1
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• pp.1-7
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• 2011

Secondary walls have recently drawn research interest as a primary source of sugars for liquid biofuel production. Secondary walls are composed of a complex mixture of the structural polymers cellulose, hemicellulose, and lignin. A matrix of hemicellulose and lignin surrounds the cellulose component of the plant's cell wall in order to protect the cell from enzymatic attacks. Such resistance, along with the variability seen in the proportions of the major components of the mixture, presents process design and operating challenges to the bioconversion of lignocellulosic biomass to fuel. Expanding bioenergy production to the commercial scale will require a significant improvement in the growth of feedstock as well as in its quality. Plant biotechnology offers an efficient means to create "targeted" changes in the chemical and physical properties of the resulting biomass through pathway-specific manipulation of metabolisms. The successful use of the genetic engineering approach largely depends on the development of two enabling tools: (1) the discovery of regulatory genes involved in key pathways that determine the quantity and quality of the biomass, and (2) utility promoters that can drive the expression of the introduced genes in a highly controlled manner spatially and/or temporally. In this review, we summarize the current understanding of the transcriptional regulatory network that controls secondary wall biosynthesis and discuss experimental approaches to developing-xylem-specific utility promoters.

• Journal of Labour Economics
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• v.19 no.2
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• pp.161-174
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• 1996

Much of the dual labor markets literature is devoted to exploring the reasons as th why the markets are segmented along the lines where the observed wage differentials are not a result of underlying skill differentials . ; and why otherwise comparable workers different the duration of their job tenure and incidence of unemployment. the logic of competitive economics denies the possibility of equally skilled workers being treated differently in labor markets. The model presented in this paper shows that workers could be segregated quite simply due to the structure of information and job-match quality, even though they are the same in terms of productivity. In general, the model predicts that observability of a worker's productivity and the extent of match specificity are key features of labor market segmentation. An important implication is that the negative from the past labor market experienes, sometimes called as hysteresis effect, helps to restrict mobility of workers among different sectors and results in perpetuation of unemployment in the secondary sector. The model also provides an explanation of the efficient wage scheme in the primary sector.

• Journal of Environmental Science International
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• v.8 no.1
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• pp.101-106
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• 1999

The relationship between primary productivity and changes in water quality was investigated at Mulgum station, a site downstream of the Nakdong River, Korea. Phytoplankton production was characterized by blooms of Microcystis aeruginosa during the summer and Stephanodiscus hantzschii during the winter. Primary production and secondary production by bacterioplankton ranged from 1.5~53.5 mg-C/ι day and 0.1~0.3 mg-C/ι day, respectively. Distribution of total organic carbon appeared to be highly correlated with phytoplankton biomass, especially during blooms of M. aeruginosa, when particulate organic carbon was 81% of total organic carbon and the main source of organic materials supplied into the water. The correlation coefficient between chlorophyll-a and BOD was 0.86. Thus it was concluded that autochthonous phytoplankton mostly affected the BOD level. Total bacterial numbers were also highly correlated with chlorophyll-a ($r^2$= 0.84) and the bacterial community appears to be regulated by phytoplankton biomass in this area.

• East Asian Journal of Business Economics (EAJBE)
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• v.5 no.2
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• pp.48-54
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• 2017

Public holidays are periods set aside by nations or states to observe particular events like national days, independence anniversary or religious festivals. These days are different from the usual periods of statutory leave or vacation provided for workers in the collective agreement or rule of engagement at the point of entry. These periods of leave enjoyed by workers include annual vacation, casual leave, sick leave, maternity leave, paternity leave and others as may be recognized within an establishment. Individual worker's period of leave is personal and at the discretion of the worker or the organization as the case may be, but public holidays involve the stoppage of work by the entire workforce within the period it would last. Nigeria observes many public holidays some of which, at times, are unplanned for by the people. This has attracted arguments for and against on the basis that these holidays are too many and detrimental to productivity and the national economy. Using secondary data, this paper examines the issue and opines that there is the need to review these public holidays with a view to halting their negative effects on the nation's economy. Also, measures to manage these holidays in line with constitutional provisions are espoused.

• Applied Biological Chemistry
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• v.48 no.1
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• pp.1-15
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• 2005

Pathogens, insects and weeds have significantly reduced agricultural productivity. Thus, to increase the productivity, synthetic agricultural chemicals have been overused. However, these synthetic compounds that are different from natural products cannot be broken down easily in natural systems, causing the destruction of soil quality and agricultural environments and the gradually difficulty in continuous agriculture. Now agriculture is faced with the various problems of minimizing the damage in agricultural environments, securing the safety of human health, while simultaneously increasing agricultural productivity. Meanwhile, plants produce secondary metabolites to protect themselves from external invaders and to secure their region for survival. Plants infected with pathogens produce antibiotics phytoalexin; monocotyledonous plants produce flavonoids and diterpenoids phytoalexins, and dicotylodoneous plant, despite of infected pathogens, produce family-specific phytoalexin such as flavonoids in Leguminosae, indole derivatives in Cruciferae, sesquitepenoids in Solanaceae, coumarins in Umbelliferae, making the plant resistant to specific pathogen. Growth inhibitor or antifeedant substances to insects are terpenoids pyrethrin, azadirachtin, limonin, cedrelanoid, toosendanin and fraxinellone/dictamnine, and terpenoid-alkaloid mixed compounds sesquiterpene pyridine and norditerpenoids, and azepine-, amide-, loline-, stemofoline-, pyrrolizidine-alkaloids and so on. Also plants produces the substances to inhibit other plant growths to secure the regions for plant itself, which is including terpenoids essential oil and sesquiterpene lactone, and additionally, benzoxazinoids, glucosinolate, quassinoid, cyanogenic glycoside, saponin, sorgolennone, juglone and lots of other different of secondary metabolites. Hence, phytoalexin, an antibiotic compound produced by plants infected with pathogens, can be employed for pathogen control. Terpenoids and alkaloids inhibiting insect growth can be utilized for insect control. Allelochemicals, a compound released from a certain plant to hinder the growth of other plants for their survival, can be also used directly as a herbicides for weed control as well. Therefore, the use of the natural secondary metabolites for pest control might be one of the alternatives for environmentally friendly agriculture. However, the natural substances are destroyed easily causing low the pest-control efficacy, and also there is the limitation to producing the substances using plant cell. In the future, effects should be made to try to find the secondary metabolites with good pest-control effect and no harmful to human health. Also the biosynthetic pathways of secondary metabolites have to be elucidated continuously, and the metabolic engineering should be applied to improve transgenics having the resistance to specific pest.

• 한국연소학회:학술대회논문집
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• 2014.11a
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• pp.339-342
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• 2014

Rotary kiln produces lime from limestone through thermal decomposition. Ring formation in kiln internal wall is known issue that decreases productivity. The cause of ring formation is temperature imbalance as flame leans toward upper wall. Therefore, burner nozzle geometry was changed to improve air-fuel mixing state which leads to prevention of ring formation. CFD simulation and experimental test were performed in this study to investigate the effect of air-fuel mixing on flame structure, temperature and $NO_X$ concentration. It is shown that combustion efficiency has been enhanced and $NO_X$ concentration has been decreased by using swirl flow for secondary combustion air. It's also shown that flame has been straightened by using straight flow for secondary combustion air.