[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.1501.01001

Whole Cell Bioconversion of Ricinoleic Acid to 12-Ketooleic Acid by Recombinant Corynebacterium glutamicum-Based Biocatalyst

Lee, Byeonghun (Department of Chemical and Biomolecular Engineering, Sogang University)
Lee, Saebom (Department of Chemical and Biomolecular Engineering, Sogang University)
Kim, Hyeonsoo (Department of Chemical and Biomolecular Engineering, Sogang University)
Jeong, Kijun (Department of Chemical and Biomolecular Engineering, KAIST)
Park, Jinbyung (Department of Food Science and Engineering, Ewha Woman's University)
Park, Kyungmoon (Department of Biological and Chemical Engineering, Hongik University)
Lee, Jinwon (Department of Chemical and Biomolecular Engineering, Sogang University)

Publication Information

Journal of Microbiology and Biotechnology / v.25, no.4, 2015 , pp. 452-458 More about this Journal

Abstract

The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35℃, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12-ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicum-based biocatalyst.

Keywords

12-Ketooleic acid; ricinoleic acid; secondary alcohol dehydrogenase; whole cell bioconversion; Corynebacterium glutamicum;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)

Reference
Cited By KSCI

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