• 한국산림과학회지
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• 제84권2호
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• pp.151-165
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• 1995

한국산(韓國産) 옻나무과(科) 6식종(植種)과 외래(外來) 2식종(植種)을 대상(對象)으로 잎의 형태학적(形態學的) 형질조사(形質調査), 엽맥상(葉脈相)의 특징(特徵) 조사(調査), 주사전자현미경(走査電子顯微鏡)을 이용하여 기공(氣孔)과 잎의 epidermal 세포(細胞)의 구조특성(構造特性)을 조사(調査)하였다. 연구결과(硏究結果)에 대한 분류군간(分類群間)의 각 형질(形質)을 비교(比較)하였으며, 결과(結果)를 요약(要約)하면 다음과 같다. 1. 잎의 형태적(形態的) 형질(形質)에 의(依)한 유집분석(類集分析) 결과(結果) 산검양옻나무, 미국털옻나무, 검양옻나무 유집군(類集群)과 개옻나무, 붉나무, 옻나무 유집군(類集群) 및 덩굴옻나무, 미국덩굴옻나무 유집군(類集群)으로 분류(分類)되었다. 엽형질(葉形質)에 대(對)한 주성분(主成分) 분석(分析)에서는 Eigenvalue 1.0이상(以上)인 제(第)3 주성분(主成分)까지의 누적기여율(累積寄與率)은 82.6%로서 설명력(說明力)이 높았다. 2. 엽맥(葉脈)의 형태(形態)와 2차맥(次脈)의 분지각(分枝角)에 따라 망상(網狀), 곡주맥(曲走脈)과 방사상(放射狀) 곡주맥(曲走脈)으로 구분(區分)되었으며, 2차맥(次脈)의 발달(發達) 정도(程度)에 따라 종(種)의 식별(識別)이 가능(可能)하였으며, 종검색표(種檢索表)를 작성(作成)하였다. 3. 기공(氣孔) 형태(形態)는 paracytic과 anomocytic형(形)으로 구별(區別)되었으며, 기공지수(氣孔指數)에 따라 종간(種間)의 분류(分類)가 가능(可能)하였다. 또한 잎 표면세포(表面細胞)의 구조(構造)와 형태(形態)도 synclinal과 anticlinal cell wall pattern으로 구분(區分)되어 종간(種間)의 차이(差異)가 인정(認定)되었다.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2005년도 제25회 추계학술대회논문집
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• pp.46-55
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• 2005

For the sea-level performance test of rocket motor designed to operate in the upper atmosphere, ejectors with no induced secondary flow are generally used, which serves dual purposes of evacuating the test cell and performing as a supersonic exhaust diffuser (SED). The main concern of this research is to simulate starting transients in order to visualize evolution of internal shock structures in SED with different initial cell (vacuum chamber) pressures. RANS code with low Reynolds $k-\varepsilon$ turbulence model was employed for these computations. Numerical results were compared with the pressure measurements previously performed [Proceedings of 2004 Annual Conference, KIMST], and showed good agreements with pressure-time history of measured data. In the case of low vacuum chamber pressure, abrupt impingement of the under-expanded supersonic jet from the nozzle onto the diffuser wall was observed, whereas initial impingement point was located downstream and moved slowly upstream in the case of non-vacuum chamber pressure. In spite of initially dissimilar evolution of shock structures, iso-mach contour revealed that the steady shock structures had little difference except the location of flow separation and normal shock.

• Journal of the Korean Wood Science and Technology
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• 제24권1호
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• pp.68-74
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• 1996

Present work was undertaken to investigate the origin of milled wood lignin(MWL) in the wood cell wall using immunocytochemical techniques, which can provide the information on the localization of specific antigens(MWL in the present study) to be examined. Spruce MWL dissolved in DMSO and emulsified with Freund adjuvant was injected directly into the mouse spleen. The animals were boostered at two-week intervals after the initial immunization. Blood samples were purified in standard procedures. The characteristics of antibodies against MWL were tested by indirect ELISA. Visualization of MWL was carried out using conventional indirect immunogold-labelling methods on the ultrathin sections of spruce wood. Immuno-TEM observations showed that the immunogold probes were selectively attached to secondary cell walls of spruce wood. The most intense labelling was frequently observed in the S2 layer. In contrast, gold labelling in the lignin-rich regions, such as middle lamella and cell corner was not found. The immuno-TEM provides an indication that spruce MWL originates from the S2 layer.

• 한국추진공학회지
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• 제6권1호
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• pp.71-82
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• 2002

본 연구에서는 2차원의 압축성 Navier-Stokes 코드를 개발하여 mixer-ejector 노즐의 유동장 해석을 다양한 덕트와 노즐 면적비 및 노즐 압력비에 대하여 계산을 수행하였다. 덕트와 노즐 면적비 계산에서는 먼저 효율적인 2차 유동의 유입을 위한 최적의 면적비가 있음을 볼 수 있었다. 높은 면적비에서는 입구 자유유동의 적절한 혼합없이 mixing duct를 그대로 통과하는 것을 볼 수 있었고, 낮은 면적비에서는 제트의 경계가 유입 유동에 장애물로 작용하는 것을 볼 수 있었다. 노즐 압력비의 계산에 있어서는 shroud 벽면과 shock cell structure 간에 상호작용이 작다면 유입유량은 압력비에 따라 증가하는 것을 볼 수 있었다.

• 대한기계학회논문집B
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• 제23권8호
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• pp.1063-1071
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• 1999

Fine grid calculations are reported for the developing turbulent flow in a curved duct of square cross-section with a radius of curvature to hydraulic diameter ratio ${\delta}=Rc/D_H=3.357 $ and a bend angle of 720 deg. A sequence of modeling refinements is introduced; the replacement of wall function by a fine mesh across the sublayer and a low Reynolds number algebraic second moment closure up to the near wall sublayer in which the non-linear return to isotropy model and the cubic-quasi-isotropy model for the pressure strain are adopted; and the introduction of a multiple source model for the exact dissipation rate equation. Each refinement is shown to lead to an appreciable improvement in the agreement between measurement and computation.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 춘계학술대회논문집A
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• pp.900-907
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• 2000

The three-dimensional turbulent flow in curved pipes susceptible to flow-accelerated corrosion has been analyzed numerically to predict the pressure and shear stress distributions on the inner surface of the pipes. The analysis employs the body-fitted non-orthogonal curvilinear coordinate system and a standard $ {\kappa}-{\varepsilon}$ turbulence model with wall function method. The finite volume method is used to discretize the governing equations. The convection term is approximated by a high-resolution and bounded discretization scheme. The cell-centered, non-staggered grid arrangement is adopted and the resulting checkerboard pressure oscillation is prevented by the application of a modified version of momentum interpolation scheme. The SIMPLE algorithm is employed for the pressure and velocity coupling. The numerical calculations have been performed for two curved pipes with different bend angles and curvature radii, and discussions have been made on the distributions of the primary and secondary flow velocities, pressure and shear stress on the inner surface of the pipe to examine applicability of the present analysis method. As the result it is seen that the method is effective to predict the susceptible systems or their local areas where the fluid velocity or local turbulence is so high that the structural integrity can be threatened by wall thinning degradation due to flow-accelerated corrosion.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집E
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• pp.213-218
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• 2001

The characteristics of the fluid flow and mass transfer in a vertical atmospheric pressure chemical vapor deposition (APCVD) are numerically studied. In order to get the optimal process parameters for the uniformity of deposition on a substrate, Navier-Stokes and energy equations have been solved for the pressure, mass-flow rate and temperature distribution in a CVD reactor. Results show that the thermal boundary condition at the reactor wall has an important effect in the formation of buoyancy-driven secondary cell when radiation effect is considered. Results also show that reduction of the buoyancy effect on the heated reactor improves the uniformity of deposition.

• Applied Microscopy
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• 제44권2호
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• pp.41-46
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• 2014

In this study, the vegetative and reproductive morphology and anatomy of two Hawaiian endemic Portulaca species were examined. Specifically, P. molokiniensis and P. sclerocarpa were compared to closely related species in the genus. The comparisons were both qualitative and quantitative, using characteristics of leaves, stems, roots, and fruits. Tissue organizations of vegetative and reproductive parts of the plants were assessed using microtechnique procedures, statistical analysis, and scanning electron microscopy. The most notable features of these two species were (1) the size and frequency of stomata in P. molokiniensis, and (2) the large number of sclerenchymatous cell layers in the thickest fruit walls of P. sclerocarpa. These findings may imply that stomata development in P. molokiniensis and thick fruit wall development in P. sclerocarpa are evolved features of survival. In particular, the development of thickened walls in indehiscent fruits likely has evolutionary implications of ecological tolerance for better adaptation.

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 2001년도 유체기계 연구개발 발표회 논문집
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• pp.334-338
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• 2001

In order to investigate the effects of various injection hole shapes on the film cooling of turbine blade, three test models having cylindrical and shaped holes were used. A three-dimensional Navier-Stokes code with standard k-$\epsilon$ model was used to compute the film cooling coefficient on the film cooled turbine blade. Over 330,000 grids were used to compute the flow over the blade. Mainstream Reynolds number based on the cylinder diameter was $7.1{\times}10^4$. The turbulence intensity kept at $5.0\%$ for all inlets. The effect of coolant blowing ratio was studied for various blowing ratios. For each blowing ratios, wall temperatures around the surface of test model were calculated. Temperature was visualized using cartesian cut-cell method to obtain traces of the injected secondary air on the test surface, so we could interpret the film effectiveness as temperature distributions.

• Molecules and Cells
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• 제42권4호
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• pp.363-375
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• 2019

Fungal sectorization is a complex trait that is still not fully understood. The unique phenotypic changes in sporadic sectorization in mutants of CpBck1, a mitogen-activated protein kinase kinase kinase (MAPKKK) gene, and CpSlt2, a mitogen-activated protein kinase (MAPK) gene, in the cell wall integrity pathway of the chestnut blight fungus Cryphonectria parasitica have been previously studied. Although several environmental and physiological factors cause this sectoring phenotype, genetic variants can also impact this complex morphogenesis. Therefore, RNA sequencing analysis was employed to identify candidate genes associated with sectorization traits and understand the genetic mechanism of this phenotype. Transcriptomic analysis of CpBck1 and CpSlt2 mutants and their sectored progeny strains revealed a number of differentially expressed genes (DEGs) related to various cellular processes. Approximately 70% of DEGs were common between the wild-type and each of CpBck1 and CpSlt2 mutants, indicating that CpBck1 and CpSlt2 are components of the same MAPK pathway, but each component governs specific sets of genes. Functional description of the DEGs between the parental mutants and their sectored progenies revealed several key pathways, including the biosynthesis of secondary metabolites, translation, amino acid metabolism, and carbohydrate metabolism; among these, pathways for secondary metabolism and translation appeared to be the most common pathway. The results of this comparative study provide a better understanding of the genetic regulation of sector formation and suggest that complex several regulatory pathways result in interplays between secondary metabolites and morphogenesis.